Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,400,966 A→C I41S (ATT→AGT)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,400,9660AC98.8% 297.9 / ‑3.9 82I41S (ATT→AGT) mreBcell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (32/49);  total (32/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

AGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGG  >  NC_000913/3400827‑3401113
                                                                                                                                           |                                                                                                                                                   
aGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGTGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACg                                                                                                                                          >  2:560023/1‑151 (MQ=255)
aGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCcaccac                                                                                                                                           <  2:55041/150‑1 (MQ=255)
 gTCACGAAGAAGTCGGCGATAACGCCGCCTTTCATTGGGCGAATGGCAGCAATATTGACCGGCGTTCGGCCCAGCATCTGCTTCGCGTCATGCCCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGccacca                                                                                                                                            <  2:1095518/148‑1 (MQ=255)
  tCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACgg                                                                                                                                         >  2:1069085/1‑150 (MQ=255)
     cGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAgg                                                                                                                                     <  1:218364/151‑1 (MQ=255)
     cGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAgg                                                                                                                                     >  1:416124/1‑151 (MQ=255)
      gaagaaGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGc                                                                                                                                    <  2:536456/151‑1 (MQ=255)
        agaagTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCt                                                                                                                                   <  2:955668/150‑1 (MQ=255)
         gaagTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGTATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTc                                                                                                                                  >  2:733820/1‑150 (MQ=255)
           agTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCAtt                                                                                                                               <  2:710126/151‑1 (MQ=255)
              cGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCa                                                                                                                             <  2:1051740/150‑1 (MQ=255)
               ggCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGAATCTGCTGCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCaa                                                                                                                            <  2:581750/150‑1 (MQ=255)
                gCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATa                                                                                                                          <  1:708086/151‑1 (MQ=255)
                      aCGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGc                                                                                                                    <  1:1064099/151‑1 (MQ=255)
                       cGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGcc                                                                                                                   <  1:252044/151‑1 (MQ=255)
                        gCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCt                                                                                                                  >  2:1189315/1‑151 (MQ=255)
                         ccGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCtt                                                                                                                 <  2:1128610/151‑1 (MQ=255)
                           gTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGt                                                                                                               >  2:534738/1‑151 (MQ=255)
                             cTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTcc                                                                                                             >  2:1057955/1‑151 (MQ=255)
                              tttCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCt                                                                                                            >  2:268415/1‑151 (MQ=255)
                                     gggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACAt                                                                                                     >  1:591298/1‑151 (MQ=255)
                                      ggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATa                                                                                                    <  1:97386/151‑1 (MQ=255)
                                          aaTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATg                                                                                                >  1:40868/1‑151 (MQ=255)
                                           aTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATg                                                                                                >  2:637128/1‑150 (MQ=255)
                                              gcagcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAggg                                                                                            <  2:217143/151‑1 (MQ=255)
                                                agcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTa                                                                                          <  1:772564/151‑1 (MQ=255)
                                                     tatTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGc                                                                                     >  1:59033/1‑151 (MQ=255)
                                                        tGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGt                                                                                  >  1:123144/1‑151 (MQ=255)
                                                         gCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGt                                                                                  <  1:129343/150‑1 (MQ=255)
                                                         gCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGt                                                                                  <  1:471100/150‑1 (MQ=255)
                                                         gCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTa                                                                                 <  1:49667/151‑1 (MQ=255)
                                                          cccGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAc                                                                                <  2:659290/151‑1 (MQ=255)
                                                           ccagCGTACGACCCAGCATCTGCTTCGCGTCATGACCTACAGCAGCTACGCTTTTCGGTAAACCGGCACGATCCTGACGACTGGCCCCCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAc                                                                                <  2:47684/147‑1 (MQ=255)
                                                             gccGTAAGACCCAGCATCTGCTTCGCGTCATGACCTACTGCCGCTACGCTTTTCGGCGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAACACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAccc                                                                              >  2:316055/3‑150 (MQ=255)
                                                                 tACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                                                          <  2:909657/150‑1 (MQ=255)
                                                                 tACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                                                          <  2:1114154/150‑1 (MQ=255)
                                                                  aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTc                                                                         >  1:864448/1‑150 (MQ=255)
                                                                   cGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCaa                                                                       <  1:784865/151‑1 (MQ=255)
                                                                    ggCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAAt                                                                      >  1:329814/1‑151 (MQ=255)
                                                                     gCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATg                                                                     <  1:683157/151‑1 (MQ=255)
                                                                       ccAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGa                                                                   <  1:260088/151‑1 (MQ=255)
                                                                        cAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAc                                                                  >  1:500995/1‑151 (MQ=255)
                                                                        cAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAc                                                                  <  1:1141507/151‑1 (MQ=255)
                                                                          gCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACa                                                                 <  1:675173/150‑1 (MQ=255)
                                                                               tGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAt                                                           >  2:154609/1‑151 (MQ=255)
                                                                                gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTc                                                             <  2:300433/148‑1 (MQ=255)
                                                                                gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAtt                                                          >  2:541029/1‑151 (MQ=255)
                                                                                         cATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAt                                                 >  1:1120758/1‑151 (MQ=255)
                                                                                            gACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGcc                                              >  1:522750/1‑151 (MQ=255)
                                                                                            gACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGc                                               <  1:509666/150‑1 (MQ=255)
                                                                                             aCCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCa                                             <  1:627966/151‑1 (MQ=255)
                                                                                             aCCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCa                                             <  2:416106/151‑1 (MQ=255)
                                                                                              ccTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCGGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCAc                                            >  2:938396/1‑151 (MQ=255)
                                                                                               cTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCAc                                            >  1:638612/1‑150 (MQ=255)
                                                                                                 aCTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg                                           <  1:788379/149‑1 (MQ=255)
                                                                                                  cTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGaa                                         <  2:446657/150‑1 (MQ=255)
                                                                                                    gCAGCTGCGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTGCCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAtt                                      <  1:179357/151‑1 (MQ=255)
                                                                                                     cggCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAt                                       <  1:560049/147‑1 (MQ=255)
                                                                                                       gCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAttttt                                   <  2:570677/151‑1 (MQ=255)
                                                                                                         tACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTc                                 >  1:746157/1‑151 (MQ=255)
                                                                                                          aCGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAtttttt                                  <  2:361114/149‑1 (MQ=255)
                                                                                                          aCGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAtttttt                                  <  1:897533/149‑1 (MQ=255)
                                                                                                          aCGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTc                                 >  2:553806/1‑150 (MQ=255)
                                                                                                           cGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCa                                >  2:541992/1‑150 (MQ=255)
                                                                                                            gCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAAc                              <  2:202850/151‑1 (MQ=255)
                                                                                                             cTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAAc                              >  1:282974/1‑150 (MQ=255)
                                                                                                               ttttGGTGAACCGGCACGATCGTGACGCCTGGCCGCCACGGAAGGTACATTCAATACGATGCCTTGTCCTTTTACAGAGATGAGGGACTTCGCAGTCCCCAGGCCAATGAACAATCCATTGGAAAACATGCCACGAAATTTTTTCAACa                             <  2:247793/149‑1 (MQ=255)
                                                                                                               tttCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACa                             <  2:1009795/149‑1 (MQ=255)
                                                                                                               tttCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACAt                            <  1:1187623/150‑1 (MQ=255)
                                                                                                                  cGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACt                         >  2:1164025/1‑150 (MQ=255)
                                                                                                                  cGGTGAACCGGCACGAGCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACt                         <  2:1115422/150‑1 (MQ=255)
                                                                                                                    gTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAAGCCACGAAATTTTTTCAACATACTAAg                      <  2:591161/151‑1 (MQ=255)
                                                                                                                       aaCCGGCACGATCCTGGCGACTGGCCACCACGGAAGGCTCATTCAATACGATTCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAAAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGa                   <  2:1025662/151‑1 (MQ=255)
                                                                                                                            gCACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATc              >  2:586740/1‑151 (MQ=255)
                                                                                                                             cACGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATcc             <  2:591269/151‑1 (MQ=255)
                                                                                                                               cGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg           >  1:420704/1‑151 (MQ=255)
                                                                                                                               cGATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg           >  2:824885/1‑151 (MQ=255)
                                                                                                                                gATCCTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGa          <  1:837522/151‑1 (MQ=255)
                                                                                                                                 agccTTACGACTGGCCACCACGGAAGGCTCATTCAATACGATGGCTTGTCCTTTTACATAAATGAGGATATTCGCATTACCCAGGTCAATGGACAAGTCATTGAAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaa         <  2:297461/149‑1 (MQ=255)
                                                                                                                                   ccTGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAg       <  1:906865/151‑1 (MQ=255)
                                                                                                                                     tGACGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCt     >  1:817149/1‑151 (MQ=255)
                                                                                                                                      gacgacTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCt     <  2:615780/150‑1 (MQ=255)
                                                                                                                                      gacgacTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCt     <  1:302942/150‑1 (MQ=255)
                                                                                                                                      gacgacTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCt     <  1:1136978/150‑1 (MQ=255)
                                                                                                                                        cGACTGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTggg  <  1:945745/151‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
AGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGG  >  NC_000913/3400827‑3401113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: