Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,579,268 | A→C | I126M (ATT→ATG) | yhhW ← | quercetinase activity in vitro |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,579,268 | 0 | A | C | 98.3% | 218.6 / ‑4.5 | 60 | I126M (ATT→ATG) | yhhW | quercetinase activity in vitro |
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (34/25); total (34/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 |
CACCGACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCTTTATTGCCCAT > NC_000913/3579124‑3579411 | cACCGACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCg > 1:972536/1‑151 (MQ=255) cGACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGttt > 2:289589/1‑151 (MQ=255) gACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGtttt > 1:1094081/1‑151 (MQ=255) gACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGtttt > 1:381768/1‑151 (MQ=255) cTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTttc < 1:131152/150‑1 (MQ=255) ttCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCAt < 1:421717/149‑1 (MQ=255) cAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAg < 2:986699/151‑1 (MQ=255) aaCGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATcc > 1:645599/1‑148 (MQ=255) gCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGAATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCTTCGAAGCGGCGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCt > 1:1068953/1‑151 (MQ=255) cccAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTg < 2:1005982/151‑1 (MQ=255) cccAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTg > 2:745646/1‑151 (MQ=255) cccAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTg > 1:1015456/1‑151 (MQ=255) cGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATAc > 1:1174086/1‑150 (MQ=255) cGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATAc > 1:766300/1‑150 (MQ=255) cGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACa < 1:437186/151‑1 (MQ=255) ggTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAg < 2:475512/151‑1 (MQ=255) ggTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGATGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAg < 1:44168/151‑1 (MQ=255) gTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATAc > 1:1017809/1‑148 (MQ=255) gTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAg < 1:187740/151‑1 (MQ=255) ttCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGc > 2:909522/1‑148 (MQ=255) tCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGa < 1:4458/150‑1 (MQ=255) atatCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGc > 2:572191/1‑150 (MQ=255) ccTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGt < 2:1189183/151‑1 (MQ=255) gATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGtgct > 2:832433/1‑151 (MQ=255) gCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGtgctgc < 1:22225/150‑1 (MQ=255) cACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACTGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTgctgct > 2:146864/1‑150 (MQ=255) cACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCtt > 2:603568/1‑151 (MQ=255) ttCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTggg < 1:1022494/150‑1 (MQ=255) tCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGGTTGCCCTGTACGGCATCGAAGCGAAGCTGTTCATAACGCGGCGTCATAACGTTTCGTTCAGGCATGATACAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGtt > 2:333647/1‑151 (MQ=255) tGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTAc > 1:518221/1‑151 (MQ=255) aaCCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTActc > 2:397506/1‑151 (MQ=255) aaCCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTAct > 2:92672/1‑150 (MQ=255) cATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTg > 2:1148807/1‑150 (MQ=255) aTCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTg > 1:833285/1‑149 (MQ=255) tctcGCGCATCCGACGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAg > 2:973735/1‑150 (MQ=255) cgcgCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAg < 1:485754/147‑1 (MQ=255) cgcgCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGt < 1:463290/148‑1 (MQ=255) cgcgCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGAc > 1:19796/1‑151 (MQ=255) gcgcATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACg > 2:153084/1‑151 (MQ=255) ggCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCg < 2:328752/151‑1 (MQ=255) gCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGt < 1:461913/151‑1 (MQ=255) cGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGt > 2:1068041/1‑150 (MQ=255) cGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGt > 2:734028/1‑150 (MQ=255) agagCGCCAGCTGTTTGCCCTGTACGGCATCGAAGTGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTGCAGGCATGATCCAGATGTGAGACAGATGCAGACGCGCGGTGCTGCTTGGGTTGTACGCTGAGTGACGAATACCCGTa < 2:950021/149‑1 (MQ=255) gagCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCa < 1:530939/151‑1 (MQ=255) gCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGc > 1:51937/1‑151 (MQ=255) gCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTg < 2:660496/150‑1 (MQ=255) aCGGCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCATACCAGCACTCATAATCTGGAACTCAc < 1:52426/151‑1 (MQ=255) ggCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCAccc < 1:67741/151‑1 (MQ=255) ggCATCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCAccc < 2:766260/151‑1 (MQ=255) aTCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACccgcc > 1:143934/1‑151 (MQ=255) aTCGAAGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACccgcc > 2:109319/1‑151 (MQ=255) cgcaGCGACGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCGGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCgg < 2:1187378/148‑1 (MQ=255) aCGCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGc > 2:957786/1‑151 (MQ=255) gCTGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGctc > 2:598353/1‑151 (MQ=255) tGTTCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCtt > 2:27582/1‑151 (MQ=255) tCATAACGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCTTTAt > 2:1151700/1‑151 (MQ=255) cATAACGCGGCGTCATCCCGTTTCCTCCAGGCATGATCCAAATCTGATACAGATGCAGACGCTCGGTGCTGTTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCTTTa < 2:58747/149‑1 (MQ=255) aaCGCGGCGTCATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCTTTATTGcc < 1:777328/151‑1 (MQ=255) cggcgTCTTACCTTTTTCTTCAGGCATGATCCAGATCTGATACATATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGATTGACGAATACCCGTCCCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCTTTATTGCCCAt < 1:855043/150‑1 (MQ=255) | CACCGACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCATGATCCAGATCTGATACAGATGCAGACGCTCGGTGCTGCTTGGGTTGTACTCTGAGTGACGAATACCCGTACCAGCACTCATAATCTGGAACTCACCCGCCGGAACCTGCTCTTTATTGCCCAT > NC_000913/3579124‑3579411 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |