Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,141	T→G	N93K (AAT→AAG)	yhhZ →	putative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,141	0	T	G	100.0%	392.9 / NA	97	N93K (AAT→AAG)	yhhZ	putative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (49/48);  total (49/48)

TAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAATAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGC  >  NC_000913/3581996‑3582287                                                                                                                                                  |                                                                                                                                                   tAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAagag                                                                                                                                                  <  1:194792/148‑1 (MQ=255) tAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAagag                                                                                                                                                  <  1:143142/148‑1 (MQ=255)  aaaTAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGAttt                                                                                                                                              >  1:948589/1‑151 (MQ=255)  aaaTAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGAttt                                                                                                                                              <  1:910994/151‑1 (MQ=255)  aaaTAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGAttt                                                                                                                                              >  1:197474/1‑151 (MQ=255)   aataatATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTg                                                                                                                                             >  2:280681/1‑151 (MQ=255)   aataatATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTg                                                                                                                                             <  2:212356/151‑1 (MQ=255)    ataataTTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTgg                                                                                                                                            >  2:997134/1‑151 (MQ=255)      aataTTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGt                                                                                                                                           >  2:33782/1‑150 (MQ=255)      aataTTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGt                                                                                                                                           >  1:132889/1‑150 (MQ=255)       atatTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAg                                                                                                                                         <  1:1177672/151‑1 (MQ=255)        tatTAATAATACGGGGCTTCGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAg                                                                                                                                         <  2:97050/150‑1 (MQ=255)            aataatACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTgg                                                                                                                                    >  2:502101/1‑151 (MQ=255)            aataatACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTgg                                                                                                                                    <  1:1008887/151‑1 (MQ=255)            aataatACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTg                                                                                                                                     <  1:1059722/150‑1 (MQ=255)              taataCGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGa                                                                                                                                   >  1:815701/1‑150 (MQ=255)               aataCGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGaaa                                                                                                                                 <  2:480280/151‑1 (MQ=255)               aataCGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGaa                                                                                                                                  <  2:529556/150‑1 (MQ=255)                ataCGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGaaaa                                                                                                                                <  2:906397/151‑1 (MQ=255)                  aCGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAg                                                                                                                               <  1:944081/150‑1 (MQ=255)                   cGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGta                                                                                                                             <  1:867410/151‑1 (MQ=255)                     gggCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGtatt                                                                                                                           <  2:1149575/151‑1 (MQ=255)                       gCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGtattat                                                                                                                         >  1:223919/1‑151 (MQ=255)                          tGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGtattattat                                                                                                                      >  1:651647/1‑151 (MQ=255)                          tGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGtattattat                                                                                                                      >  1:330173/1‑151 (MQ=255)                          tGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGtattatt                                                                                                                        <  2:645281/149‑1 (MQ=255)                          tGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGtattatt                                                                                                                        <  2:595096/149‑1 (MQ=255)                            gTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATtatat                                                                                                                    <  1:481990/151‑1 (MQ=255)                               cacaGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATAc                                                                                                                  <  1:510241/150‑1 (MQ=255)                               cacaGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATAc                                                                                                                  >  2:657664/1‑150 (MQ=255)                               cacaGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACa                                                                                                                 <  1:277778/151‑1 (MQ=255)                                   gTTCCATTGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTa                                                                                                             <  2:937733/151‑1 (MQ=255)                                     tCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTa                                                                                                             >  1:730448/1‑149 (MQ=255)                                       cATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAagag                                                                                                         <  1:1020086/151‑1 (MQ=255)                                          ggTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGgcg                                                                                                      >  1:602835/1‑151 (MQ=255)                                            tataACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCt                                                                                                    >  1:59049/1‑151 (MQ=255)                                             ataACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCtt                                                                                                   <  2:976627/151‑1 (MQ=255)                                             ataACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCtt                                                                                                   >  1:830720/1‑151 (MQ=255)                                             ataACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCt                                                                                                    <  2:574195/150‑1 (MQ=255)                                              taACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCttt                                                                                                  >  1:1174064/1‑151 (MQ=255)                                                  aTTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCtttttt                                                                                               >  2:84800/1‑150 (MQ=255)                                                  aTTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCtttttt                                                                                               >  1:1051289/1‑150 (MQ=255)                                                  aTTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTa                                                                                              <  1:428363/151‑1 (MQ=255)                                                  aTTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTa                                                                                              <  2:158447/151‑1 (MQ=255)                                                    tttGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGCATTATTATATCCAACTAAGAGTCGCTTTTTTacc                                                                                            >  2:657364/1‑149 (MQ=255)                                                      tGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGAATTATTATATACAACTAAGAGGCGCTTTTTTATCgg                                                                                          >  1:1082051/1‑151 (MQ=255)                                                          aattaattGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTAt                                                                                      >  1:590090/1‑151 (MQ=255)                                                          aattaattGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTAt                                                                                      <  1:359462/151‑1 (MQ=255)                                                              aattGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATtc                                                                                    <  1:709238/149‑1 (MQ=255)                                                               attGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATtcatc                                                                                 >  1:1077380/1‑151 (MQ=255)                                                               attGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATtcatc                                                                                 <  1:1053636/151‑1 (MQ=255)                                                               aataaTAAAAGCCCCCCATTATTTTTTAATTCCATTAACAATAATGAACCATTATGTATTGGAATTGAAGTCTTACGAAAAAAGAAGATTGGTAAATTTGAAAAGTATTGATTTATACAACTAAGAGGCGCTTTTTTATCGGCTATtcatc                                                                                 <  2:1162080/147‑1 (MQ=255)                                                                   aTAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCAcc                                                                              >  2:1176692/1‑150 (MQ=255)                                                                   aTAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCa                                                                             <  1:441159/151‑1 (MQ=255)                                                                     aaaaGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGACGCGCTTTTTTATCGGCTATTCATCAcc                                                                              >  1:661488/1‑148 (MQ=255)                                                                         gCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGTTTTTTTATCGGCTATTCATCACCAGATCAt                                                                       >  1:682748/1‑151 (MQ=255)                                                                          cACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCAtt                                                                      <  1:792888/151‑1 (MQ=255)                                                                          cACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTAATCACCAGATCAtt                                                                      >  2:58237/1‑151 (MQ=255)                                                                           aCTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTg                                                                     <  2:489393/151‑1 (MQ=255)                                                                            cTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTg                                                                     >  1:793164/1‑150 (MQ=255)                                                                                   atttattAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCa                                                              >  1:1080481/1‑150 (MQ=255)                                                                                     ttattaATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACt                                                           >  1:748571/1‑151 (MQ=255)                                                                                       attaATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTgg                                                         <  1:34742/151‑1 (MQ=255)                                                                                         taATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATATAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAACACCAACTGGAt                                                       >  2:137931/1‑151 (MQ=255)                                                                                         taATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGa                                                        >  1:150640/1‑150 (MQ=255)                                                                                          aaTTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGAt                                                       >  1:1176368/1‑150 (MQ=255)                                                                                          aaTTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGAt                                                       <  1:1055129/150‑1 (MQ=255)                                                                                               cATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGaa                                                 <  1:273046/151‑1 (MQ=255)                                                                                               cATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGaa                                                 <  1:1106831/151‑1 (MQ=255)                                                                                                   aaCAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACa                                              >  2:107178/1‑150 (MQ=255)                                                                                                     cAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAAt                                            >  1:69254/1‑150 (MQ=255)                                                                                                     cAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAAt                                            >  2:982594/1‑150 (MQ=255)                                                                                                     cAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATa                                           <  2:1124764/151‑1 (MQ=255)                                                                                                      aataatGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATa                                           >  1:452890/1‑150 (MQ=255)                                                                                                      aataatGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATa                                           >  2:1013900/1‑150 (MQ=255)                                                                                                       ataatGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAAc                                         <  2:604546/151‑1 (MQ=255)                                                                                                         aatGAACAATTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACt                                        >  1:719758/1‑150 (MQ=255)                                                                                                                aaTTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTa                                <  2:651616/151‑1 (MQ=255)                                                                                                                aaTTATTTATGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTa                                <  1:880374/151‑1 (MQ=255)                                                                                                                   tatttatGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATg                              <  1:881787/150‑1 (MQ=255)                                                                                                                   tatttatGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATg                              >  1:1148843/1‑150 (MQ=255)                                                                                                                       tatGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAAttt                         >  2:685602/1‑151 (MQ=255)                                                                                                                       tatGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAAttt                         >  2:212686/1‑151 (MQ=255)                                                                                                                        atGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTa                        <  2:850863/151‑1 (MQ=255)                                                                                                                        atGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTa                        <  2:800036/151‑1 (MQ=255)                                                                                                                         tGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTAt                       >  2:11177/1‑151 (MQ=255)                                                                                                                         tGGGATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTAt                       >  1:227695/1‑151 (MQ=255)                                                                                                                            gATTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCct                    <  1:464671/151‑1 (MQ=255)                                                                                                                             aTTTGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCctc                   <  1:621151/151‑1 (MQ=255)                                                                                                                               ttGACTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCAGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCctct                  <  2:693102/150‑1 (MQ=255)                                                                                                                                  aCTTCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTc               >  1:1156851/1‑150 (MQ=255)                                                                                                                                    ttCTATCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAAc            >  2:64588/1‑151 (MQ=255)                                                                                                                                        aTCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACa           <  2:332157/148‑1 (MQ=255)                                                                                                                                        aTCGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCt        >  1:788429/1‑151 (MQ=255)                                                                                                                                          cGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCtt       >  1:1147429/1‑150 (MQ=255)                                                                                                                                          cGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCtt       <  1:802698/150‑1 (MQ=255)                                                                                                                                          cGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCtt       >  1:935669/1‑150 (MQ=255)                                                                                                                                          cGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTa      <  2:549799/151‑1 (MQ=255)                                                                                                                                          cGAATAAAGAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTa      <  2:452869/151‑1 (MQ=255)                                                                                                                                              taaagaGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGc  >  2:443506/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATTATTTATTAATTCCATTAACAATAATGAACAATTATTTATGGGATTTGACTTCTATCGAATAAATAGATTTGGTAGATTGGAAAAGTATTATTATATACAACTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGC  >  NC_000913/3581996‑3582287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑