Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,691,406 T→G D401A (GAT→GCT)  bcsB ← regulator of cellulose synthase, cyclic di‑GMP binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,691,4060TG95.5% 228.5 / 1.7 66D401A (GAT→GCT) bcsBregulator of cellulose synthase, cyclic di‑GMP binding
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (39/24);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCCTCCTGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGATCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTACGGCTTACGCGGT  >  NC_000913/3691262‑3691549
                                                                                                                                                |                                                                                                                                               
gCCTCCTGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCcggcg                                                                                                                                           >  1:1164985/1‑151 (MQ=255)
 cctcctGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCcggcgg                                                                                                                                          >  2:950259/1‑151 (MQ=255)
 cctcctGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCcggcgg                                                                                                                                          <  1:1195761/151‑1 (MQ=255)
  ctcctGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCggcggc                                                                                                                                         >  1:110184/1‑151 (MQ=255)
    cctGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAg                                                                                                                                       <  2:1136734/151‑1 (MQ=255)
          tgctgcTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGttt                                                                                                                                   >  1:624728/1‑149 (MQ=255)
           gctgctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAg                                                                                                                                 <  2:676591/150‑1 (MQ=255)
           gctgctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGc                                                                                                                                >  1:618536/1‑151 (MQ=255)
           gcggctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGATCCGGCGGCAGGTTTAg                                                                                                                                 <  2:433558/147‑1 (MQ=255)
             tgctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCg                                                                                                                               >  1:338509/1‑150 (MQ=255)
              gctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCg                                                                                                                               <  1:403654/149‑1 (MQ=255)
              gctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGa                                                                                                                              >  1:384813/1‑150 (MQ=255)
              gctCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGa                                                                                                                              >  2:588829/1‑150 (MQ=255)
                tCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAAc                                                                                                                           >  2:1125450/1‑151 (MQ=255)
                 cAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAAc                                                                                                                           <  1:43510/150‑1 (MQ=255)
                   ggTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGtt                                                                                                                        <  1:235703/151‑1 (MQ=255)
                          ggATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCgct                                                                                                                 <  1:393968/151‑1 (MQ=255)
                                    aaCTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGGATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCa                                                                                                        <  2:125601/150‑1 (MQ=255)
                                    aaCTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCa                                                                                                        >  2:899778/1‑150 (MQ=255)
                                    aaCTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCa                                                                                                        >  2:637723/1‑150 (MQ=255)
                                       tGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCTGGGAGAGATCCGGCCGCAGGCTTAGCGAAACGTTCATCGCTGCTGGCTCa                                                                                                        >  2:718708/1‑147 (MQ=255)
                                       tGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGAc                                                                                                    >  1:1203467/1‑151 (MQ=255)
                                                aGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGAtt                                                                                           >  1:351389/1‑151 (MQ=255)
                                                aGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGAtt                                                                                           >  1:1118921/1‑151 (MQ=255)
                                                      atatCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACt                                                                                     >  1:34391/1‑151 (MQ=255)
                                                       tatCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACt                                                                                     >  2:504743/1‑150 (MQ=255)
                                                          ccatccGCGAACTGTCTTTCACCGGCGGCAAGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGttc                                                                                 <  1:678125/151‑1 (MQ=255)
                                                            atccGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGttctt                                                                               >  2:758972/1‑151 (MQ=255)
                                                             tccGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGttcttc                                                                              >  1:580226/1‑151 (MQ=255)
                                                                gcgAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCAt                                                                            >  1:850620/1‑150 (MQ=255)
                                                                   aaCTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGt                                                                        >  1:215807/1‑151 (MQ=255)
                                                                   aaCTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGt                                                                        >  1:204799/1‑151 (MQ=255)
                                                                    aCTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGtt                                                                       <  1:512371/151‑1 (MQ=255)
                                                                       gTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGttt                                                                      <  1:672523/149‑1 (MQ=255)
                                                                       gTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTc                                                                    >  2:330699/1‑151 (MQ=255)
                                                                       gTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTc                                                                    <  1:371540/151‑1 (MQ=255)
                                                                             cACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCg                                                              >  2:127773/1‑151 (MQ=255)
                                                                             cACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCg                                                              >  1:738648/1‑151 (MQ=255)
                                                                               ccGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGc                                                             <  2:730028/150‑1 (MQ=255)
                                                                                    ggcATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAAtg                                                       >  1:30536/1‑151 (MQ=255)
                                                                                     gcATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAAtgt                                                      <  1:945481/151‑1 (MQ=255)
                                                                                      cATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAAtgtg                                                     >  1:897442/1‑151 (MQ=255)
                                                                                      cATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAAtgtg                                                     >  1:11851/1‑151 (MQ=255)
                                                                                       aTGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAAtgtg                                                     >  1:1113614/1‑150 (MQ=255)
                                                                                         ggTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGAcc                                                  >  2:987566/1‑151 (MQ=255)
                                                                                         ggTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGAcc                                                  >  1:1205376/1‑151 (MQ=255)
                                                                                           tgtAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCgg                                                >  1:934303/1‑151 (MQ=255)
                                                                                            gtAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCgg                                                <  1:987360/150‑1 (MQ=255)
                                                                                            gtAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGa                                               <  2:1207615/151‑1 (MQ=255)
                                                                                               gCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGa                                            <  2:536045/151‑1 (MQ=255)
                                                                                                cGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGTGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGAt                                           <  1:144456/151‑1 (MQ=255)
                                                                                                cGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGAt                                           >  1:339630/1‑151 (MQ=255)
                                                                                                 ggTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATc                                          <  1:153565/151‑1 (MQ=255)
                                                                                                 ggTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATc                                          <  2:709547/151‑1 (MQ=255)
                                                                                                    aattaatATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGgt                                       <  2:1119151/151‑1 (MQ=255)
                                                                                                       taatATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGgtacg                                    >  2:426623/1‑151 (MQ=255)
                                                                                                             ccATATCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTAccc                               >  2:137834/1‑150 (MQ=255)
                                                                                                                  tCAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCg                         >  2:559763/1‑151 (MQ=255)
                                                                                                                   cAATGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCgg                        >  1:87811/1‑151 (MQ=255)
                                                                                                                     aTGCCGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGgcg                      >  1:103483/1‑151 (MQ=255)
                                                                                                                         cGGTACTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATc                  >  2:277718/1‑151 (MQ=255)
                                                                                                                             aCTGCGCATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTa               <  1:2821/150‑1 (MQ=255)
                                                                                                                                gcgcATCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTACGGc           <  1:492523/151‑1 (MQ=255)
                                                                                                                                    aTCAGGTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTACGGCTTAc       >  1:761614/1‑151 (MQ=255)
                                                                                                                                         gTAGAGATCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTACGGCTTACGCGGt  <  1:253486/151‑1 (MQ=255)
                                                                                                                                         gTAGAGAGCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTACGGCTTACGCGGt  <  1:936975/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCCTCCTGTTTGCTGCTCAGGTTGAAGGATTGCAGGAACTGGTTATTCAGGCTGATATCCATCCGCGAACTGTCTTTCACCGGCGGCATGGTGTAGCGGTAATTAATATCCATATCAATGCCGGTACTGCGCATCAGGTAGAGATCCGGCGGCAGGTTTAGCGAAACGTTAATCGCTGCTGGCTCAAGACCGCTGGATTGTAACTGTTCTTCATAGGTTTTCAGTTCGCCAAATGTGACCGGACGATCGGTACGTACCCAGTTCGGCGCATCGTACGGCTTACGCGGT  >  NC_000913/3691262‑3691549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: