Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,891,019 T→G S262A (TCC→GCC)  tnaB → tryptophan transporter of low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,891,0190TG98.7% 296.2 / ‑3.6 79S262A (TCC→GCC) tnaBtryptophan transporter of low affinity
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (42/36);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

CCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTGG  >  NC_000913/3890873‑3891166
                                                                                                                                                  |                                                                                                                                                   
ccAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATcgcctc                                                                                                                                                 >  1:1134352/1‑151 (MQ=255)
 cAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCa                                                                                                                                                <  1:917079/151‑1 (MQ=255)
  aGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATcgcctc                                                                                                                                                 >  1:962868/1‑149 (MQ=255)
  aGCCTGATTACTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATcgcctc                                                                                                                                                 >  1:1115522/1‑149 (MQ=255)
     cTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAggcg                                                                                                                                            >  2:998347/1‑151 (MQ=255)
     cTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAggcg                                                                                                                                            >  2:452267/1‑151 (MQ=255)
        attattTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCa                                                                                                                                         <  2:1072280/151‑1 (MQ=255)
         ttattTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCa                                                                                                                                         <  2:619418/150‑1 (MQ=255)
            tttGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGt                                                                                                                                     >  2:432188/1‑151 (MQ=255)
             ttGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGtt                                                                                                                                    >  1:31856/1‑151 (MQ=255)
             ttGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGt                                                                                                                                     <  1:547697/150‑1 (MQ=255)
               gCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTg                                                                                                                                   >  1:760788/1‑150 (MQ=255)
                 tATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGAtt                                                                                                                                >  1:468003/1‑151 (MQ=255)
                  aTGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGACTATTCCGCGAGAAAGCATTAAGGCGATTATCGCCTCAGGCGGCAACGTTGAtt                                                                                                                                >  2:906021/1‑150 (MQ=255)
                     gAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCt                                                                                                                            >  1:1210608/1‑151 (MQ=255)
                     gAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCt                                                                                                                            <  2:944030/151‑1 (MQ=255)
                      aaaaCGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTg                                                                                                                           <  1:189931/151‑1 (MQ=255)
                       aaaCGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTgg                                                                                                                          <  1:841190/151‑1 (MQ=255)
                           gCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGa                                                                                                                       >  2:430102/1‑150 (MQ=255)
                            cAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGaa                                                                                                                      >  2:332486/1‑150 (MQ=255)
                             aaaGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAAt                                                                                                                    <  1:109277/151‑1 (MQ=255)
                               aGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCg                                                                                                                  >  1:576650/1‑151 (MQ=255)
                                gATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGt                                                                                                                 >  2:975446/1‑151 (MQ=255)
                                     gTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTc                                                                                                            <  2:1210530/151‑1 (MQ=255)
                                       taataaAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCgg                                                                                                          >  2:374462/1‑151 (MQ=255)
                                       tAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCg                                                                                                           <  1:1097166/150‑1 (MQ=255)
                                         aTCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCg                                                                                                           <  1:442697/148‑1 (MQ=255)
                                           cAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAc                                                                                                       <  1:894805/150‑1 (MQ=255)
                                            aaaaGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAcc                                                                                                      >  2:578453/1‑150 (MQ=255)
                                                gCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACa                                                                                                 <  1:761069/151‑1 (MQ=255)
                                                 cGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAg                                                                                                <  1:1134766/151‑1 (MQ=255)
                                                  gTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGc                                                                                               >  1:12061/1‑151 (MQ=255)
                                                  gTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGc                                                                                               >  2:348670/1‑151 (MQ=255)
                                                    ggTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCa                                                                                              <  1:756894/150‑1 (MQ=255)
                                                       aTTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACgg                                                                                           <  2:209678/150‑1 (MQ=255)
                                                          tGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCAt                                                                                        <  1:109191/150‑1 (MQ=255)
                                                           ggTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCATCGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCCCGGCATTa                                                                                      <  2:934987/151‑1 (MQ=255)
                                                           ggTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTa                                                                                      <  1:1109245/151‑1 (MQ=255)
                                                                gctgctGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCg                                                                                   >  1:192643/1‑149 (MQ=255)
                                                                     tgGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTg                                                                            >  1:396565/1‑151 (MQ=255)
                                                                      ggCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTg                                                                            <  1:749431/150‑1 (MQ=255)
                                                                      ggCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGc                                                                           <  1:918231/151‑1 (MQ=255)
                                                                        cgcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCct                                                                         <  2:480242/151‑1 (MQ=255)
                                                                        cgcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGCGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGcc                                                                          >  1:849915/1‑150 (MQ=255)
                                                                         gcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCct                                                                         >  1:150889/1‑150 (MQ=255)
                                                                               gATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTgg                                                                    >  1:722263/1‑149 (MQ=255)
                                                                               gATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGgtg                                                                  <  2:463530/151‑1 (MQ=255)
                                                                               gATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGgt                                                                   <  1:735357/150‑1 (MQ=255)
                                                                                   tATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTtct                                                              >  1:992710/1‑151 (MQ=255)
                                                                                   tATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTtct                                                              >  1:384304/1‑151 (MQ=255)
                                                                                        cttctGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTctct                                                            <  2:757971/148‑1 (MQ=255)
                                                                                          tctGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACtt                                                       >  2:133494/1‑151 (MQ=255)
                                                                                          tctGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACtt                                                       >  2:35745/1‑151 (MQ=255)
                                                                                             ggCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGc                                                    <  1:293780/151‑1 (MQ=255)
                                                                                              gCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCt                                                   >  1:781344/1‑151 (MQ=255)
                                                                                                tctATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGt                                                 >  2:548056/1‑151 (MQ=255)
                                                                                                 ctATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTg                                                  <  2:746231/149‑1 (MQ=255)
                                                                                                 ctATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTg                                                  <  2:8376/149‑1 (MQ=255)
                                                                                                   aTTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGc                                              >  2:400993/1‑151 (MQ=255)
                                                                                                         ccATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttc                                        >  1:192846/1‑151 (MQ=255)
                                                                                                          cATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcg                                       <  2:19759/151‑1 (MQ=255)
                                                                                                             ggggAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAgttcgttc                                    >  2:175818/1‑151 (MQ=255)
                                                                                                                gAATATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCttt                                 <  2:621432/151‑1 (MQ=255)
                                                                                                                 aaTATTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTg                                <  2:171180/151‑1 (MQ=255)
                                                                                                                   tatTCCGCGAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGAAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGgt                              >  2:756040/1‑151 (MQ=255)
                                                                                                                        cgcgAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCAc                         >  1:60359/1‑151 (MQ=255)
                                                                                                                         gcgAGAAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACg                        >  2:1177944/1‑151 (MQ=255)
                                                                                                                            agaAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCGCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCt                      <  1:934493/150‑1 (MQ=255)
                                                                                                                             gaAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTgg                    >  1:77826/1‑151 (MQ=255)
                                                                                                                             gaAAGCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTgg                    >  1:1203986/1‑151 (MQ=255)
                                                                                                                                 gCTTTAAGGCGATTTTTGCCGCTTTCGTCACCGCTCGTTCGCCCGTGAAATCGTTCCTCGGCACCAAACCGCACGTCATTATCGAGTTTTCCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCACTTCGTTCTTTGGTGTCACGCTGTGgtt                >  2:505461/1‑151 (MQ=255)
                                                                                                                                 gCTTTAAGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGgtt                <  2:156727/151‑1 (MQ=255)
                                                                                                                                      aaGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCg           <  2:203883/151‑1 (MQ=255)
                                                                                                                                      aaGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCg           >  1:798135/1‑151 (MQ=255)
                                                                                                                                       aGGCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCg           >  2:184185/1‑150 (MQ=255)
                                                                                                                                         gCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGAtt        >  1:203526/1‑151 (MQ=255)
                                                                                                                                         gCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGAtt        >  1:547797/1‑151 (MQ=255)
                                                                                                                                         gCGATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGAtt        >  1:449314/1‑151 (MQ=255)
                                                                                                                                           gATTATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTAt      <  2:1052785/151‑1 (MQ=255)
                                                                                                                                              tATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCt    <  2:1007280/150‑1 (MQ=255)
                                                                                                                                              tATCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTg   >  1:951354/1‑151 (MQ=255)
                                                                                                                                               aTCGCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTgg  >  2:1111718/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAGCTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTGG  >  NC_000913/3890873‑3891166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: