Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,860	A→C	F107V (TTT→GTT)	rarD ←	putative chloramphenical resistance permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,860	0	A	C	100.0%	261.2 / NA	69	F107V (TTT→GTT)	rarD	putative chloramphenical resistance permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/30);  total (39/30)

CAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGC  >  NC_000913/4003714‑4004006                                                                                                                                                   |                                                                                                                                                   cAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTaa                                                                                                                                                <  1:710515/151‑1 (MQ=255) cAGCGCGATAATAGGCAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTa                                                                                                                                                 >  2:272511/1‑150 (MQ=255)   gcgcGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAAc                                                                                                                                               <  2:1092248/150‑1 (MQ=255)   gcgcGATAATAGGAAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGGAAcc                                                                                                                                              >  2:868069/1‑151 (MQ=255)     gcgATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCa                                                                                                                                             >  1:1003757/1‑150 (MQ=255)         taataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGc                                                                                                                                         >  2:374912/1‑150 (MQ=255)          aataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTc                                                                                                                                       >  1:279657/1‑151 (MQ=255)             aGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGctctct                                                                                                                                    <  2:547875/151‑1 (MQ=255)             aGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCt                                                                                                                                    >  2:1059563/1‑151 (MQ=255)                tAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTc                                                                                                                                  >  2:189566/1‑150 (MQ=255)                  gCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAg                                                                                                                               <  1:996880/151‑1 (MQ=255)                     aaCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCat                                                                                                                            >  1:607064/1‑151 (MQ=255)                      aCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCat                                                                                                                            >  1:966081/1‑150 (MQ=255)                         aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATgg                                                                                                                        >  1:1093920/1‑151 (MQ=255)                         aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATg                                                                                                                         <  1:335426/150‑1 (MQ=255)                         aaaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATg                                                                                                                         <  2:338103/150‑1 (MQ=255)                           aaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTg                                                                                                                      <  2:999773/151‑1 (MQ=255)                            aGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGa                                                                                                                     <  2:31824/151‑1 (MQ=255)                                   aGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTACTAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt                                                                                                                <  2:986505/149‑1 (MQ=255)                                    gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCAc                                                                                                             <  1:53464/151‑1 (MQ=255)                                     cTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCAcc                                                                                                            >  1:193462/1‑151 (MQ=255)                                      tGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCg                                                                                                           >  1:232542/1‑151 (MQ=255)                                       ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCTCAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCg                                                                                                           <  2:202740/150‑1 (MQ=255)                                       ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGc                                                                                                          <  2:1100368/151‑1 (MQ=255)                                           tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                                                                        >  1:1169386/1‑149 (MQ=255)                                            aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGccc                                                                                                        >  2:1205060/1‑148 (MQ=255)                                            aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCAGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGcc                                                                                                         >  1:617623/1‑147 (MQ=255)                                             acacGCCACATATCGCAAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGAt                                                                                                    <  2:806912/151‑1 (MQ=255)                                                cGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATaaa                                                                                                 <  1:824280/151‑1 (MQ=255)                                                cGCCACATAGCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATa                                                                                                   <  2:1066926/149‑1 (MQ=255)                                                     caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGGATCGCTCGCCGAGGAACATCATCCCCAGCACAATGTTCACCAGCGGGGTAATAACGTAACCAAGGCGCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAg                                                                                              >  2:251295/1‑149 (MQ=255)                                                      atatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAg                                                                                              >  1:773692/1‑148 (MQ=255)                                                      atatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAg                                                                                           <  1:803732/151‑1 (MQ=255)                                                       tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTa                                                                                            <  1:192134/149‑1 (MQ=255)                                                           gCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAg                                                                                       >  2:225285/1‑150 (MQ=255)                                                              aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                                                                    >  2:825328/1‑150 (MQ=255)                                                              aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                                                                    <  2:973700/150‑1 (MQ=255)                                                               gAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTg                                                                                    <  2:902202/149‑1 (MQ=255)                                                                   cACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGccacca                                                                              <  1:880497/151‑1 (MQ=255)                                                                          agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                                                                        >  1:45578/1‑150 (MQ=255)                                                                          agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGc                                                                        >  2:546907/1‑150 (MQ=255)                                                                             caTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACgg                                                                    >  2:457544/1‑151 (MQ=255)                                                                                tGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGc                                                                   >  2:896284/1‑149 (MQ=255)                                                                                tGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCa                                                                  <  1:1056377/150‑1 (MQ=255)                                                                                   aTCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCag                                                                 >  2:124654/1‑148 (MQ=255)                                                                                          ggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAg                                                       <  2:561560/151‑1 (MQ=255)                                                                                               cgctcgcCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATa                                                   >  1:769977/1‑150 (MQ=255)                                                                                                    gcCGAGGAAAATCATCCCCAGCACAATGTGCACCAGCGGGTTAATAACGTAGCCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATaaaaa                                               <  2:929098/149‑1 (MQ=255)                                                                                                     ccGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAAAAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGACAGCATAAAAAttt                                            >  2:1169123/1‑151 (MQ=255)                                                                                                          gAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCtgt                                       >  1:1126323/1‑151 (MQ=255)                                                                                                           ataaTCATCCCCAGCACAATGATCACCACCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGAAAAACAGTAGCCAGTTGCCACCAATTAGCATGGAAGAGACTGTCAGCATAAAAATTTTCtgtg                                      >  2:660954/3‑151 (MQ=255)                                                                                                           aaaaTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCtgtg                                      >  2:611556/1‑151 (MQ=255)                                                                                                               tcatcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGt                                  >  2:90945/1‑151 (MQ=255)                                                                                                               tcatcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGt                                  >  1:85731/1‑151 (MQ=255)                                                                                                                catcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGt                                  <  2:11092/150‑1 (MQ=255)                                                                                                                 atcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCt                                <  2:40989/151‑1 (MQ=255)                                                                                                                 atcCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCt                                >  2:229207/1‑151 (MQ=255)                                                                                                                   ccccAGCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTg                               >  1:915843/1‑150 (MQ=255)                                                                                                                        gCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAg                         <  2:1126248/151‑1 (MQ=255)                                                                                                                        gCACAATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAg                         <  2:183673/151‑1 (MQ=255)                                                                                                                          acaATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCg                       >  1:814075/1‑151 (MQ=255)                                                                                                                          acaATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCg                       <  1:1045915/151‑1 (MQ=255)                                                                                                                           caATGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGt                      <  1:1053904/151‑1 (MQ=255)                                                                                                                              tGTTCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGtttt                   >  2:849515/1‑151 (MQ=255)                                                                                                                                ttCACCAGCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGttttt                  >  2:498012/1‑150 (MQ=255)                                                                                                                                  cACCAGCGGGCTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCTGCTGAATCAGCGTTTTTaa                >  2:10452/1‑150 (MQ=255)                                                                                                                                       gCGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGa          <  1:480661/151‑1 (MQ=255)                                                                                                                                        cGGGTTAATAACGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGAc         >  2:993285/1‑151 (MQ=255)                                                                                                                                               ataaCGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTggc  >  2:973269/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAGCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGC  >  NC_000913/4003714‑4004006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑