Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,256,722	A→C	E29A (GAA→GCA)	dgkA →	diacylglycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,256,722	0	A	C	100.0%	304.2 / NA	81	E29A (GAA→GCA)	dgkA	diacylglycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (43/38);  total (43/38)

CCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGA  >  NC_000913/4256575‑4256866                                                                                                                                                    |                                                                                                                                                 ccGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAg                                                                                                                                                >  1:299924/1‑150 (MQ=255) ccGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGc                                                                                                                                               <  2:91351/151‑1 (MQ=255) ccGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGGTTACGCGCTGCATGGATCAACGCAg                                                                                                                                                >  2:63228/1‑150 (MQ=255)   gAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCgg                                                                                                                                             <  2:491167/151‑1 (MQ=255)   gAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCgg                                                                                                                                             >  1:144033/1‑151 (MQ=255)     atcatcGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCa                                                                                                                                           <  2:1085383/151‑1 (MQ=255)        atcGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTc                                                                                                                                        >  1:210674/1‑151 (MQ=255)          cGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTcc                                                                                                                                       <  2:501935/150‑1 (MQ=255)          cGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCg                                                                                                                                      >  1:124941/1‑151 (MQ=255)          cGGATTTAACGGTACAATGATATTGACGCTCATAATGTAAAAAGGGTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCCACGCAGCGGCATTCCg                                                                                                                                      >  2:765227/1‑151 (MQ=255)             aTTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTc                                                                                                                                    >  1:834999/1‑150 (MQ=255)                             atatTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGt                                                                                                                    >  1:1065031/1‑150 (MQ=255)                              tatTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAt                                                                                                                  >  2:323911/1‑151 (MQ=255)                              tatTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAt                                                                                                                  >  1:471235/1‑151 (MQ=255)                                         aTAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtg                                                                                                       >  1:1087141/1‑151 (MQ=255)                                         aTAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggt                                                                                                        >  2:702536/1‑150 (MQ=255)                                          tAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtgg                                                                                                      >  1:403156/1‑151 (MQ=255)                                           aaTGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtggt                                                                                                     >  2:679441/1‑151 (MQ=255)                                           aaTGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtgg                                                                                                      >  1:392985/1‑150 (MQ=255)                                              gTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCAt                                                                                                  <  2:1827/151‑1 (MQ=255)                                               tAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATc                                                                                                 >  2:1067935/1‑151 (MQ=255)                                                   aaGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCct                                                                                             >  2:878657/1‑151 (MQ=255)                                                        tCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTgctgg                                                                                        >  1:934324/1‑151 (MQ=255)                                                          tttCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTgctggct                                                                                      >  1:738970/1‑151 (MQ=255)                                                          tttCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTgctggc                                                                                       >  1:1192948/1‑150 (MQ=255)                                                             cAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGa                                                                                   <  2:77222/151‑1 (MQ=255)                                                              aaTGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGctggctgg                                                                                    >  2:670526/1‑149 (MQ=255)                                                                tGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAtgt                                                                                >  1:809987/1‑151 (MQ=255)                                                                 ggCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAtgtg                                                                               >  1:633813/1‑151 (MQ=255)                                                                  gCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTgg                                                                              >  1:92002/1‑151 (MQ=255)                                                                   ccAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGa                                                                             <  1:218096/151‑1 (MQ=255)                                                                   ccAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGa                                                                             <  1:980722/151‑1 (MQ=255)                                                                    cAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAc                                                                            <  1:785849/151‑1 (MQ=255)                                                                    cAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAc                                                                            >  1:528526/1‑151 (MQ=255)                                                                      ataataCCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAcgc                                                                          <  1:922082/151‑1 (MQ=255)                                                                      ataataCCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAcgc                                                                          >  1:91317/1‑151 (MQ=255)                                                                        aataCCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAcgcg                                                                         <  1:638728/150‑1 (MQ=255)                                                                        aataCCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAcgcg                                                                         <  2:342825/150‑1 (MQ=255)                                                                        aataCCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGAcgcg                                                                         >  2:318171/1‑150 (MQ=255)                                                                                 ggATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCcgcg                                                               <  1:33910/151‑1 (MQ=255)                                                                                 ggATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCcg                                                                 <  1:558449/149‑1 (MQ=255)                                                                                  gATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGt                                                              <  1:1122742/151‑1 (MQ=255)                                                                                     tCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGtgct                                                           >  1:403168/1‑151 (MQ=255)                                                                                      cACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGtgct                                                           <  2:809338/150‑1 (MQ=255)                                                                                       aCCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGtgctgc                                                         >  1:227287/1‑151 (MQ=255)                                                                                        cccGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTgctgct                                                        <  1:827198/151‑1 (MQ=255)                                                                                        cccGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTgctgct                                                        >  1:960082/1‑151 (MQ=255)                                                                                         ccGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCtt                                                       <  1:646445/151‑1 (MQ=255)                                                                                         ccGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCtt                                                       <  2:550601/151‑1 (MQ=255)                                                                                               tATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAg                                                  <  2:299912/150‑1 (MQ=255)                                                                                                 tCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTc                                               >  2:68655/1‑151 (MQ=255)                                                                                                  cAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTcc                                              >  1:507997/1‑151 (MQ=255)                                                                                                  cAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTcc                                              >  1:114004/1‑151 (MQ=255)                                                                                                   aaaGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTcc                                              <  1:738134/150‑1 (MQ=255)                                                                                                   aaaGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCg                                             >  2:466832/1‑151 (MQ=255)                                                                                                    aaGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGt                                            <  2:646944/151‑1 (MQ=255)                                                                                                         gctgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGAt                                         <  2:419648/149‑1 (MQ=255)                                                                                                           tgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGAATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCCTGATGCTg                                     >  2:921757/1‑151 (MQ=255)                                                                                                            ggCTATTCCTGGAAAGGTTTACGCTCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCGTCACCTGCTGGCTGGATGTGGACGCGATTGCCCGCGTGCTTCGTATGAGCTCGGTGATGCTgg                                    <  2:674861/151‑1 (MQ=255)                                                                                                            ggCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCt                                      <  1:748081/149‑1 (MQ=255)                                                                                                            ggCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTg                                     <  2:315634/150‑1 (MQ=255)                                                                                                              cTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtg                                  >  1:732827/1‑151 (MQ=255)                                                                                                                aTTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtgat                                <  1:702570/151‑1 (MQ=255)                                                                                                                aTTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtga                                 <  2:1131302/150‑1 (MQ=255)                                                                                                                 ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtgatg                               >  2:520116/1‑151 (MQ=255)                                                                                                                 ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtga                                 >  1:976403/1‑149 (MQ=255)                                                                                                                   ccTGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTgatgat                             <  2:130742/151‑1 (MQ=255)                                                                                                                     tGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtgat                                <  2:1069372/146‑1 (MQ=255)                                                                                                                     tGGAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATtg                           >  1:391012/1‑151 (MQ=255)                                                                                                                      ggAAAGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATtgt                          <  2:641667/151‑1 (MQ=255)                                                                                                                          aGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGaa                      <  2:463512/151‑1 (MQ=255)                                                                                                                          aGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGa                       >  1:88876/1‑150 (MQ=255)                                                                                                                          aGGTTTACGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGGCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGa                       >  2:947509/1‑150 (MQ=255)                                                                                                                                aCGCGCTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCt                 <  2:35176/150‑1 (MQ=255)                                                                                                                                   cgcTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAAt             <  1:231956/151‑1 (MQ=255)                                                                                                                                    gcTGCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATa            <  1:71327/151‑1 (MQ=255)                                                                                                                                       gCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAgc          <  1:215352/150‑1 (MQ=255)                                                                                                                                       gCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAg           <  2:385576/149‑1 (MQ=255)                                                                                                                                       gCATGGATCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAg           <  2:966886/149‑1 (MQ=255)                                                                                                                                             aTCAACGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCg   >  1:465560/1‑151 (MQ=255)                                                                                                                                                aaCGCAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGa  >  2:524149/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGA  >  NC_000913/4256575‑4256866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑