Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,304,847 T→G N584T (AAC→ACC)  yjcS ← metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,304,8470TG98.7% 284.4 / ‑3.5 79N584T (AAC→ACC) yjcSmetallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (39/38);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

TTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTG  >  NC_000913/4304699‑4304992
                                                                                                                                                    |                                                                                                                                                 
tttGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTg                                                                                                                                                 >  1:570901/1‑151 (MQ=255)
    gCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAgg                                                                                                                                              <  2:110328/150‑1 (MQ=255)
     cTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCCTCGGCCTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCCGGGGGAgg                                                                                                                                              >  2:841899/1‑149 (MQ=255)
      tttCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGCCGTGCAGATCTTCACGGCTGATGAAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGGTCAGCGTCAGGGTGAGGt                                                                                                                                             >  1:548020/1‑149 (MQ=255)
       ttcttcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTa                                                                                                                                           <  2:956003/150‑1 (MQ=255)
        tcttcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCCGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTAt                                                                                                                                          >  1:790586/1‑150 (MQ=255)
        tcttcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTAt                                                                                                                                          <  1:857785/150‑1 (MQ=255)
           tcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATc                                                                                                                                         <  2:797851/148‑1 (MQ=255)
                 ttACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGc                                                                                                                                 >  1:982787/1‑150 (MQ=255)
                 ttACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGGTATCGCCGGTg                                                                                                                                  >  2:758296/1‑149 (MQ=255)
                  tACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGc                                                                                                                                 >  1:268592/1‑149 (MQ=255)
                   aCCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGc                                                                                                                                 >  2:334285/1‑148 (MQ=255)
                    ccAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCat                                                                                                                             >  2:1064286/1‑151 (MQ=255)
                     cAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCata                                                                                                                            <  1:72072/151‑1 (MQ=255)
                     cAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCat                                                                                                                             >  2:608553/1‑150 (MQ=255)
                     cAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCat                                                                                                                             >  2:441314/1‑150 (MQ=255)
                        aTCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATAtt                                                                                                                          >  1:237031/1‑150 (MQ=255)
                         tCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTg                                                                                                                         >  2:366018/1‑150 (MQ=255)
                         tCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTg                                                                                                                         >  2:203909/1‑150 (MQ=255)
                             ccATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGtt                                                                                                                    <  1:854564/151‑1 (MQ=255)
                                 tttGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATTTAGAAAGAGGCGCCGGCTTGCGGTTGCAGCGGTTTCCGGCAGTTAAGCACGCTATCGTTCCGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGaa                                                                                                                       >  2:8522/1‑144 (MQ=255)
                                        tGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGata                                                                                                          <  1:1095179/150‑1 (MQ=255)
                                        tGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGata                                                                                                          <  2:74894/150‑1 (MQ=255)
                                           ccGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATAtttt                                                                                                      >  2:397912/1‑151 (MQ=255)
                                            cGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATAttttt                                                                                                     <  2:261714/151‑1 (MQ=255)
                                            cGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATAttttt                                                                                                     <  1:615004/151‑1 (MQ=255)
                                             ggTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTa                                                                                                    <  1:1055844/151‑1 (MQ=255)
                                              gTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTAc                                                                                                   <  1:965468/151‑1 (MQ=255)
                                                 aGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCg                                                                                                <  1:214836/151‑1 (MQ=255)
                                                  gCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGc                                                                                               <  1:411811/151‑1 (MQ=255)
                                                    aCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGc                                                                                               >  1:48984/1‑149 (MQ=255)
                                                         gTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTc                                                                                        <  1:709295/151‑1 (MQ=255)
                                                            cAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTcgcg                                                                                     >  1:1205780/1‑151 (MQ=255)
                                                              gATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCgcgc                                                                                    <  1:997363/150‑1 (MQ=255)
                                                                   tCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCGGGCTGATATTTTTACCCGCAGCTTTCGCGCTa                                                                                  <  2:1111236/147‑1 (MQ=255)
                                                                   tCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCt                                                                                   <  1:640654/146‑1 (MQ=255)
                                                                   tCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTAt                                                                                 <  1:788603/148‑1 (MQ=255)
                                                                    cACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAg                                                                             <  1:1009855/151‑1 (MQ=255)
                                                                    cACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCg                                                                               >  1:273378/1‑149 (MQ=255)
                                                                    cACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAg                                                                             <  2:1024664/151‑1 (MQ=255)
                                                                      cGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCGGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGc                                                                           >  2:1070285/1‑151 (MQ=255)
                                                                       ggCTGATGTAGAAAGAGTCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCATGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCg                                                                          <  2:570066/151‑1 (MQ=255)
                                                                       ggCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGc                                                                           <  1:156817/150‑1 (MQ=255)
                                                                              gTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTATTTAAGCACTCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGCTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGcc                                                                   >  2:350817/1‑151 (MQ=255)
                                                                                  aaaGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATaa                                                               <  1:765199/151‑1 (MQ=255)
                                                                                  aaaGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATa                                                                >  2:1097165/1‑150 (MQ=255)
                                                                                   aaGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATaaa                                                              <  1:473102/151‑1 (MQ=255)
                                                                                          gTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGaa                                                        >  1:26404/1‑150 (MQ=255)
                                                                                            cGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTGCGCGCTATCGAGGCGAACGGCCATAAAGTCGAGCa                                                      <  2:881773/150‑1 (MQ=255)
                                                                                            cGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTGCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAg                                                     <  2:530289/151‑1 (MQ=255)
                                                                                              gCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGc                                                    >  1:245660/1‑150 (MQ=255)
                                                                                              gCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCa                                                   <  1:855143/151‑1 (MQ=255)
                                                                                                ttGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCAtt                                                 >  1:259214/1‑151 (MQ=255)
                                                                                                  gCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTc                                               <  1:493793/151‑1 (MQ=255)
                                                                                                   cGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcg                                              <  2:619211/151‑1 (MQ=255)
                                                                                                   cGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcg                                              <  2:467714/151‑1 (MQ=255)
                                                                                                    ggTTGCAGCGTTTTCCGGTAGTTAAGCGCGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcg                                              <  2:871553/150‑1 (MQ=255)
                                                                                                    ggTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcg                                              >  2:761680/1‑150 (MQ=255)
                                                                                                     gTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcgac                                            <  2:733277/151‑1 (MQ=255)
                                                                                                       tGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcgacc                                           <  1:300675/150‑1 (MQ=255)
                                                                                                        gCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcgaccg                                          <  2:1202985/150‑1 (MQ=255)
                                                                                                         cAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTcgaccgac                                        >  1:533544/1‑151 (MQ=255)
                                                                                                          aGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACa                                       <  1:600547/151‑1 (MQ=255)
                                                                                                           gCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACAt                                      >  2:88074/1‑151 (MQ=255)
                                                                                                             gTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATcc                                    >  1:1176092/1‑151 (MQ=255)
                                                                                                              ttttCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATcc                                    <  2:52074/150‑1 (MQ=255)
                                                                                                                  ccGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGATGAGGTTATCGCCGGTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCcgcg                                <  2:1179261/150‑1 (MQ=255)
                                                                                                                    ggTAGTTAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAAt                             >  2:1077602/1‑151 (MQ=255)
                                                                                                                         ttAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGgtgt                        >  1:220339/1‑151 (MQ=255)
                                                                                                                          tAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTc                       >  1:270612/1‑151 (MQ=255)
                                                                                                                          tAAGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTc                       >  1:1198533/1‑151 (MQ=255)
                                                                                                                           aaGCACGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTcc                      >  2:300683/1‑151 (MQ=255)
                                                                                                                               aCGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACCGCATTTCGACCGACATCCCGCGAATGGTGTCCgggg                  >  2:878436/1‑151 (MQ=255)
                                                                                                                               aCGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCgggg                  >  1:828549/1‑151 (MQ=255)
                                                                                                                                cGCTATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCgggg                  >  1:212709/1‑150 (MQ=255)
                                                                                                                                   tATCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAAcc              >  2:36976/1‑151 (MQ=255)
                                                                                                                                    aTCGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCg             >  1:476989/1‑151 (MQ=255)
                                                                                                                                      cGTTCAGCGTCAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCggt           <  2:570875/151‑1 (MQ=255)
                                                                                                                                                cAGGGTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTg  <  2:926823/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTG  >  NC_000913/4304699‑4304992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: