Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,246	A→C	D311A (GAT→GCT)	nadR →	trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,246	0	A	C	100.0%	245.1 / NA	66	D311A (GAT→GCT)	nadR	trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/28);  total (38/28)

ATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTC  >  NC_000913/4628102‑4628391                                                                                                                                                 |                                                                                                                                                  atGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTg                                                                                                                                              <  2:106720/150‑1 (MQ=255)   ggCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTca                                                                                                                                           <  1:929264/151‑1 (MQ=255)   ggCCGCGATTATGTCTTTTCACACCTCGGCGGCGGTGAGATCGCATTGCAGTATTCTGACTCCGATAAAATCGCGCTGGGCCCCGCTCCATACATTGATTTTGCGGTGAAATATGCCCATAAAGTGGCATTTATCGATACCGCCTTTGTc                                                                                                                                            >  2:371007/1‑150 (MQ=255)    gCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTcac                                                                                                                                          <  2:240654/151‑1 (MQ=255)      cgcgATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACACTCAATACATTGAATTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTcacc                                                                                                                                         >  1:571633/1‑150 (MQ=255)        cgATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGAATACGATAAAATCGCGCTGGGCCACGCTCCATACCTTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGACACCGCTTTTGTcaccac                                                                                                                                       >  2:1050610/1‑150 (MQ=255)          aTTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCa                                                                                                                                    <  2:11449/151‑1 (MQ=255)           ttATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACCATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTc                                                                                                                                     >  2:442759/1‑149 (MQ=255)             aTGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCCCGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGTCATTTATCGATACCGCTTTTGTCACCACTCAgg                                                                                                                                  <  1:569028/150‑1 (MQ=255)                tCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGGGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGt                                                                                                                               >  1:791237/1‑150 (MQ=255)                   tttCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGtt                                                                                                                              <  2:451029/148‑1 (MQ=255)                     tCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGGGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTg                                                                                                                           >  2:663117/1‑149 (MQ=255)                       acacCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATAAATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCaa                                                                                                                        >  2:178633/1‑150 (MQ=255)                         acCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCaaaaa                                                                                                                     <  2:21000/151‑1 (MQ=255)                             cggcggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTAc                                                                                                                 <  2:218791/151‑1 (MQ=255)                              ggcggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACg                                                                                                                >  2:163684/1‑151 (MQ=255)                                 ggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGa                                                                                                               >  2:949566/1‑149 (MQ=255)                                       gagaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCggg                                                                                                       <  2:427283/151‑1 (MQ=255)                                               aTTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCg                                                                                               >  2:414197/1‑151 (MQ=255)                                                  gCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGtt                                                                                             <  1:1747/150‑1 (MQ=255)                                                    aGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGt                                                                                          >  1:422910/1‑151 (MQ=255)                                                    aGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGt                                                                                          <  1:482898/151‑1 (MQ=255)                                                     gTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTg                                                                                         >  1:980955/1‑151 (MQ=255)                                                     gTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTg                                                                                         >  2:760958/1‑151 (MQ=255)                                                       aTTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTATGCGGTGAAATATGCCAATAAAGTGGAATTTATCGATACCGCTTTTGGCACCACTCAGGCTTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGc                                                                                        <  2:448918/150‑1 (MQ=255)                                                        ttCTGACTACGATAAAATCGCGCTGGGCCACGCTCCATACATTGATTGTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCACTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGGAGGGCGGGAACATCCGTTCGTGc                                                                                        >  1:819330/1‑149 (MQ=255)                                                          cTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgc                                                                                    >  2:673088/1‑151 (MQ=255)                                                            gACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgcgc                                                                                  <  1:580956/151‑1 (MQ=255)                                                                  gATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgattg                                                                            <  1:734634/151‑1 (MQ=255)                                                                   aTAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgattg                                                                            >  2:471249/1‑150 (MQ=255)                                                                     aaaaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATtgatg                                                                         <  2:197602/151‑1 (MQ=255)                                                                      aaaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATttatga                                                                        <  2:44876/151‑1 (MQ=255)                                                                        aTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAAt                                                                      >  1:338254/1‑151 (MQ=255)                                                                                gggCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATGCCGtt                                                                <  2:964598/149‑1 (MQ=255)                                                                                gggCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTc                                                              <  1:421304/151‑1 (MQ=255)                                                                                    cccGCTCAATACATTGTTTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTATGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATc                                                          >  2:719888/3‑151 (MQ=255)                                                                                     aCGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCt                                                         >  1:33143/1‑151 (MQ=255)                                                                                      cGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATGCCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCt                                                         <  2:1172601/150‑1 (MQ=255)                                                                                      cGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTg                                                        <  2:47329/151‑1 (MQ=255)                                                                                        cTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGt                                                      >  1:1059932/1‑151 (MQ=255)                                                                                         tCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTg                                                     >  2:999232/1‑151 (MQ=255)                                                                                          cAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTg                                                     >  1:242459/1‑150 (MQ=255)                                                                                           aaTACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTg                                                     <  1:1028673/149‑1 (MQ=255)                                                                                           aaTACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGAt                                                   >  1:398628/1‑151 (MQ=255)                                                                                           aaTACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGAt                                                   >  1:570465/1‑151 (MQ=255)                                                                                              aCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATcc                                                 <  2:117260/150‑1 (MQ=255)                                                                                                  tGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCctgct                                             <  1:1182405/150‑1 (MQ=255)                                                                                                    aTTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                           >  2:1138630/1‑150 (MQ=255)                                                                                                        tGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaa                                       >  2:860669/1‑150 (MQ=255)                                                                                                         gCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGgag                                         >  1:319616/1‑147 (MQ=255)                                                                                                          cGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaaca                                     >  1:537975/1‑150 (MQ=255)                                                                                                            gTGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGaacaac                                   >  1:714492/1‑150 (MQ=255)                                                                                                             tGAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAacac                                 >  2:2877/1‑151 (MQ=255)                                                                                                              gAAATATGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACg                                >  1:1139267/1‑151 (MQ=255)                                                                                                                  tatGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgt                            >  1:1179670/1‑151 (MQ=255)                                                                                                                   atGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgt                            <  1:442780/150‑1 (MQ=255)                                                                                                                    tGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtgg                          >  1:925010/1‑151 (MQ=255)                                                                                                                    tGCCAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtgg                          >  1:200994/1‑151 (MQ=255)                                                                                                                       cAATAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtgggtg                       >  1:137893/1‑151 (MQ=255)                                                                                                                          tAAAGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCg                    >  2:155330/1‑151 (MQ=255)                                                                                                                            aaGTGGCATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGa                  <  2:422890/151‑1 (MQ=255)                                                                                                                                  cATTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt            <  2:53592/151‑1 (MQ=255)                                                                                                                                   aTTTATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTa           <  2:819301/151‑1 (MQ=255)                                                                                                                                     ttATCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACg         >  2:504474/1‑151 (MQ=255)                                                                                                                                       aTCGATACCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGc        >  2:567155/1‑150 (MQ=255)                                                                                                                                             aCCGCTTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGCTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTc  <  1:1120754/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  ATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTC  >  NC_000913/4628102‑4628391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑