Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,416	T→G	F137C (TTT→TGT)	creB →	response regulator in two‑component regulatory system with CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,416	0	T	G	100.0%	205.7 / NA	56	F137C (TTT→TGT)	creB	response regulator in two‑component regulatory system with CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/32);  total (24/32)

CGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGA  >  NC_000913/4636270‑4636560                                                                                                                                                   |                                                                                                                                                 cGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTgtgaa                                                                                                                                              <  1:185760/151‑1 (MQ=255)     cGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTgtgaattg                                                                                                                                           <  2:935318/150‑1 (MQ=255)     cGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTgtgaattg                                                                                                                                           >  2:894233/1‑150 (MQ=255)      gCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTgtgaattgaa                                                                                                                                         <  2:644810/151‑1 (MQ=255)      gCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTgtgaattga                                                                                                                                          >  1:984315/1‑150 (MQ=255)         tGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGGAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATtgaatg                                                                                                                                       >  2:952329/1‑150 (MQ=255)            ttGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAAcc                                                                                                                                   >  2:674545/1‑151 (MQ=255)              gggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCcg                                                                                                                                 <  1:949478/151‑1 (MQ=255)               ggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCcgc                                                                                                                                <  1:800001/151‑1 (MQ=255)                     aaaTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGgcgc                                                                                                                           >  2:544396/1‑150 (MQ=255)                     aaaTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCa                                                                                                                          <  1:901590/151‑1 (MQ=255)                     aaaTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCa                                                                                                                          <  2:1124310/151‑1 (MQ=255)                          ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATc                                                                                                                      >  1:157583/1‑150 (MQ=255)                          ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCa                                                                                                                     <  2:343686/151‑1 (MQ=255)                          ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCa                                                                                                                     <  1:645985/151‑1 (MQ=255)                           gTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAg                                                                                                                    <  2:1158934/151‑1 (MQ=255)                             gCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCt                                                                                                                  <  1:512251/151‑1 (MQ=255)                                gacgacTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGt                                                                                                               <  1:919828/151‑1 (MQ=255)                                 acgacTACGTGGCTAAAGCTTTTGCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGTGTCGGGTGAATAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTAAATGAACCCGCGGCGCAGATCAGCTGGtt                                                                                                              <  2:262101/151‑1 (MQ=255)                                 acgacTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGtt                                                                                                              >  2:393543/1‑151 (MQ=255)                                  cgacTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGttt                                                                                                             <  1:996504/151‑1 (MQ=255)                                        cGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACg                                                                                                       >  1:420151/1‑151 (MQ=255)                                         gTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGc                                                                                                      <  2:837398/151‑1 (MQ=255)                                           ggCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCa                                                                                                    <  1:314796/151‑1 (MQ=255)                                               aacccGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGCCACGCCATTa                                                                                                 <  2:70167/147‑1 (MQ=255)                                                     ttttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTg                                                                                           >  1:472930/1‑150 (MQ=255)                                                        tCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTc                                                                                       >  1:889429/1‑151 (MQ=255)                                                          aCCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCgg                                                                                     >  2:592898/1‑151 (MQ=255)                                                           ccccGCGAAGTGTGCGCCAGGTTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCGCCCGTCATCCGTATTGGACATTGTGAATTGAATGACCCCGCGGCGAAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGt                                                                                    <  1:12651/151‑1 (MQ=255)                                                             ccGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTAt                                                                                  >  1:202472/1‑151 (MQ=255)                                                               gggAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGa                                                                                <  2:330436/149‑1 (MQ=255)                                                               gcgAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGa                                                                                <  2:657188/151‑1 (MQ=255)                                                                cgAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATg                                                                                 >  1:376939/1‑149 (MQ=255)                                                                  aaGTGTGCGCCAGGGTGCGCACCTTACGGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGtt                                                                             <  2:1176859/151‑1 (MQ=255)                                                                      gtgCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGttttt                                                                          >  2:868755/1‑150 (MQ=255)                                                                      gtgCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACCCGGTATGAGTTTTTa                                                                         <  2:235604/151‑1 (MQ=255)                                                                          gcCAGGGTGCGCACCTTACTGCGTGGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAtt                                                                       <  1:786777/149‑1 (MQ=255)                                                                          gcCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAttgt                                                                     >  1:988082/1‑151 (MQ=255)                                                                           ccAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAttgtt                                                                    <  1:555943/151‑1 (MQ=255)                                                                           ccAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAttgtt                                                                    <  2:88930/151‑1 (MQ=255)                                                                               ggTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAg                                                                <  1:794827/151‑1 (MQ=255)                                                                                     cACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGtt                                                           >  1:395544/1‑150 (MQ=255)                                                                                     cACCTTACGGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGAAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTa                                                          <  1:326783/151‑1 (MQ=255)                                                                                           aCTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCa                                                      >  1:387338/1‑149 (MQ=255)                                                                                            cTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGAAATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAg                                                    <  2:1095368/150‑1 (MQ=255)                                                                                             tGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTc                                                  >  1:814044/1‑151 (MQ=255)                                                                                               cGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCAc                                                >  1:799796/1‑151 (MQ=255)                                                                                                  cGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGGATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCgg                                             >  1:261023/1‑151 (MQ=255)                                                                                                    ggTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCgg                                             >  2:6392/1‑149 (MQ=255)                                                                                                            agTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTg                                   >  2:854137/1‑151 (MQ=255)                                                                                                             gTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTGTGAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTgg                                  <  1:651721/151‑1 (MQ=255)                                                                                                                          tctcCCGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAAc                     >  1:1136572/1‑151 (MQ=255)                                                                                                                             cccGTCATCCGTGTTGAACATTGTAAATTAAATGACCCCGCGGGGATGATCAGCGGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGa                  <  2:458840/151‑1 (MQ=255)                                                                                                                             cccGTCATCCGTATTGGACATTGTGAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACt                    <  1:1050365/149‑1 (MQ=255)                                                                                                                             cccGTCACCCGTATTGGACATTGTGAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACt                    <  1:952391/149‑1 (MQ=255)                                                                                                                              ccGTCATCCGTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGAt                 >  2:418791/1‑151 (MQ=255)                                                                                                                                    cccGTATTGGACATTGTAAATTAAATAACCCCGCGGCGCAGATCAGCTGTTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAg           <  1:762024/150‑1 (MQ=255)                                                                                                                                     ccGTTTTGGAAATTGTGATTTAAATGACCCCGCGGCGCAGATCAGCTGTTTTGACACGCCATTAGCGCTGACTCGGTATGATTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGAt             <  2:395527/148‑1 (MQ=255)                                                                                                                                       gTATTGGACATTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGt        >  2:454573/1‑151 (MQ=255)                                                                                                                                          ttGGACATTGTAAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTAt      <  1:238796/150‑1 (MQ=255)                                                                                                                                                aTTGTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGa  >  1:1092722/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGA  >  NC_000913/4636270‑4636560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑