Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6860GT41.5% 48.5 / 25.4 66intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (27/11);  new base T (11/16);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 2.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCC  >  NC_000913/2151543‑2151834
                                                                                                                                                  |                                                                                                                                                       
ataCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCtt                                                                                                                                                  <  2:574132/151‑1 (MQ=255)
  aCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTc                                                                                                                                                 <  2:95304/150‑1 (MQ=255)
  aCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCa                                                                                                                                                <  2:1200166/151‑1 (MQ=255)
                       ggcggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGCGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTTGATTTTTTTTTTTCAACCCCCAGAGaaa                                                                                                                                 >  2:839539/1‑144 (MQ=255)
                          ggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATTAGAAactcac                                                                                                                            >  1:270050/1‑146 (MQ=255)
                            ttATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTGATTTTTTTCTTTCACCGCCACGAGaaaaatacctttccc                                                                                                                     >  2:434228/1‑139 (MQ=255)
                               taCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTTATTGTTGTCTTCAACCGCCATTGGAAaattactcatccc                                                                                                                     >  1:75704/1‑143 (MQ=255)
                                    ttGATCCTTTATTGGTCACCTTCCAAAAAAAAGAAGCCTACGAGCTAAGAGAAAAATTAGTTCTCTAATCAGCGTTTATATTTTTTTTTTAAACTGGCGGTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCcaca                                                                                                                  <  2:931316/146‑1 (MQ=255)
                                        tCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTCAAACGCCCTGAGAAACTCACTATGCCCACAGCGCCAGTg                                                                                                         >  2:89214/1‑151 (MQ=255)
                                           tttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGcc                                                                                                       >  1:184373/1‑150 (MQ=255)
                                            ttATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTATTTCGCTAATCAGCCTTTATATTTATTTTTAAAACGGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa                                                                                                      <  1:260414/150‑1 (MQ=255)
                                               ttGGTCACCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCAAATCAGCCGTTATATTTATTGTTTAAACGGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACg                                                                                                   <  1:84004/150‑1 (MQ=255)
                                               tggtcccccTACCAAAAAGAAAAGCCCGACAAGAAAAAAAAAAAATTTTTCCGCTAACCAGCCTTATTATTTTTTTTTAAACCGGGGGTTTAATTTTTTGTCTAAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCaa                                                                                                     <  2:649519/141‑1 (MQ=255)
                                                tgttCACCTACCCAAAACAAGAGCCCGCGGAATTAAGACAAAAATTATTTCCTTAATCACTGGTTTTATTTTTTGTTAAAACGGTCTTTAGATTTGTTGTTTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGt                                                                                                  <  1:266427/147‑1 (MQ=255)
                                                    cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGATGTTTTCTTGTTCTCAACCGCCATTAGAAAATTACCCTCTCCCCCACCCCAGTGCCACCGttt                                                                                                >  2:171431/1‑148 (MQ=255)
                                                       cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTGTGTCTTTCACCACCATGAGAAACTCACTCTGCCCACACCGCCAGAGCCAACGCTTTat                                                                                             >  1:251977/1‑148 (MQ=255)
                                                        tATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTCAACCGCCACGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTttcat                                                                                          >  2:240348/1‑150 (MQ=255)
                                                         aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGGGCCAACGTTTTataat                                                                                          >  1:622999/1‑149 (MQ=255)
                                                           caaaaaaaAGAAGCCGACGAGCAAACAGAAAAATTATTTCGCTAATCAGCCTTTATTTTTTTTGTTAAAACGGGCGGTTGAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatca                                                                                           <  1:706012/144‑1 (MQ=255)
                                                            cAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCCCCATGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCCACGTTTTatcatcatc                                                                                      >  1:1216872/1‑150 (MQ=255)
                                                               aagaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTTCCCACAGCGCCCGTGCCAACGGTTTATCATCATCCGt                                                                                   >  1:930693/1‑150 (MQ=255)
                                                                agaagaggCCGACGAGCAAAGAGAAAAATTAGTTCGCTAATCAGCCTTTATATTTTTTTTTTAACCGGGGGGTTGAATTTTTGTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc                                                                                 <  1:391967/151‑1 (MQ=255)
                                                                    gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGATTGGTGGGTTTAACCGCCCGGGGAAAATTACTCAGCCCCAAGCGCCCGGGCCAACATTTTttcgttgtccgcac                                                                                 >  2:637302/1‑136 (MQ=255)
                                                                       gCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAAACCACTCTGCCCCCAGCGCCCAGGCCAACGGTTTATCATCCTCCGttccccc                                                                             >  1:1255408/1‑142 (MQ=255)
                                                                        cagaggaGCAAAAAGAAAATTTAGTTCGCAAACCAGCCTTTTTATTTTTTTTTAAAACGGGCGTTTGAATTTTTGCTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCt                                                                             <  2:54800/141‑1 (MQ=255)
                                                                              aGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccgcc                                                                 >  1:1088066/1‑151 (MQ=255)
                                                                               gcaaggtgCAAAATTGGTTCGCTAATCACCTGTTATATTTATTGTTAAACCGGGCGGTTAAATTTTTGTTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccgcc                                                                 <  1:362632/143‑1 (MQ=255)
                                                                               gCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAATGCCAACGTTTTATCATCATCCGTACGCCCGTTTAccgcc                                                                 >  1:296834/1‑150 (MQ=255)
                                                                                        aaaaTTAGTGCGCAAATCAGCCTTTATATTTTTTTTTAAAACGGGCGTTTGATTTGTTGTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTgg                                                          <  1:527793/148‑1 (MQ=255)
                                                                                         aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGATTGTTTTCTTTCACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATTCGTACGCTCGTTTACCGCCggg                                                              >  1:718605/1‑143 (MQ=255)
                                                                                            ttAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGTATTGTTGTCTTCAACCGCCATTAGAAAATCCCCCTGCCCACAGCGCCAGGCCCAACGGTTTATCATCATCCGTACGCTCGTTTAACGCCCGGCTGGACaaaa                                                    >  1:1281124/1‑150 (MQ=255)
                                                                                                tccgttAATCAGCCTTTATTTTTTTTTTTAAAACGGGCGTTTGATTTTTTGTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACt                                                 <  1:988947/144‑1 (MQ=255)
                                                                                                 tCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTAAACGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTTTCACCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGc                                               >  1:596445/1‑150 (MQ=255)
                                                                                                  cGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAAACGCCCTGAGAAAATCACTCTGCCCCCAGCGCCAGTGGCAACGTTTTTTCATCATCCGTACGCCCGTTTTCCCCCGGGCTGGGAAAAAAATGCtt                                             >  2:673295/1‑151 (MQ=255)
                                                                                                   gcaaaccAGCTGTTATTTTTATTTTTAAACGGGGCGTTTAATTTTTTTTCTAACACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaaa                                                   <  1:35352/138‑1 (MQ=255)
                                                                                                     tAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTTAACCCCCATGAGAAACTCACTCTGCCCCCCGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAATGCttt                                            >  2:1256851/1‑149 (MQ=255)
                                                                                                        tCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTCAACCCCCCCGAGAAACTCAATTTGCCCACAGCGCCAATGCCCAAGATTTATCATCCTCCCTGCGCTCGTTTAACGCCCGGGTGGACAAAAAATGCTTTAACa                                        >  1:710695/1‑150 (MQ=255)
                                                                                                        tCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCCACCGCCCTGAGAAACTCACTCTGCCCCCAGCGCCAGTGGCCACGCTTTATCATCATTCGTACGCTCGTTTACCCCCGGGCTGGGCAAAAACAGCttt                                            >  2:306892/1‑146 (MQ=255)
                                                                                                        tCAGCCGTTATATTTATTGTTTAAACTGGCGGCTGCATTTTTGTCTTCAACCCCCATGCGGAACTTACTCTGCCCACCACGCCCGTGCCAAAGTTTTTTCATCCTCCGTAAGCCCCGTTTCCCACCGGCTGGCCAAAAAcaacatttaca                                        >  1:1028264/1‑140 (MQ=255)
                                                                                                         cAGCCTTTTTTTTTTTTTTTAAACCGGGGGGTTGAATTGTTTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACaa                                       <  2:275753/150‑1 (MQ=255)
                                                                                                          aGCCGTTATATTTATTGTTTAAACTGGCGGTTTAAGTGTTTTCTTTTACCCCCATGAGAAACCCACTCCTTCCACAACCCCAGTGCCAACGTTTTCTCAACCTCCGTTAGCTCCATTACCCCCCGGCTGGACAAAAACAGCTTTAACaaat                                     >  1:1268904/1‑149 (MQ=255)
                                                                                                          aGCCGTTATATTTATTGTTTAAACTGGCGGTTGGAATGTTTTTTTCCACCACCCTGAGAAAAATACACTTCCCCCAGCGCCAGTGCCCACGTTTTATAATTATAAGGAAGCCCGGTTTCCCCCCGGGCGGGAAAAAAATTCTTTaa                                          >  2:688760/1‑146 (MQ=255)
                                                                                                            cctttatttttatttTTAAAACGGGGGTTTAATTTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAAtt                                     <  1:241730/146‑1 (MQ=255)
                                                                                                               ttATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAAATCACTCTGCCCACAACGCCAGTGCCCACGTTTTATTATCATCCGTACGCCCGTTTACCGCCCGGGTGGGCAAAAACTGCTTTAAAAAtttaag                                 >  1:142244/1‑146 (MQ=255)
                                                                                                                tataTTTATTGTTTAAACTGGCGGTTGGAATTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTTTCATCATCCCTACGCCCGTTTACCCCCGGGCCGGACAAAAACTGCTTTTACAAATCAgg                                 >  2:648129/1‑149 (MQ=255)
                                                                                                                 atatTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCCCGCCGCGAGGGGCAACGGTTTTTTATCATCCCTTAGCTCGTTTTCCGCCGGGGGGGAAAAAAACTGCGTTTACAAtt                                     >  1:78268/1‑144 (MQ=255)
                                                                                                                  tttttttttttagaaCTGGGGTTTTAATTTTTGTCTTCATCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaa                              <  2:1115876/137‑1 (MQ=255)
                                                                                                                  tattttttGTTTAACCGGGGGTTTAATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta                               <  1:827322/149‑1 (MQ=255)
                                                                                                                    ttttttttttAAACCGGGGGTTTAATTTTTTTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaat                             <  2:16815/150‑1 (MQ=255)
                                                                                                                     ttattGTTTAAACTGGCGGTTGCATTTTTTTCTTCCACCCCCATCCGAAACCTACTCTGCCCACAGCGCCCGTCGCCACCTTTTTTTATTACACCTACGCGCCTTTTTCGCCCGGCTGGGCCCAAAATCCTTTTACAATCTAGGgaataa                           >  2:870073/1‑150 (MQ=255)
                                                                                                                        ttGTTAAAGCGGGGGTTTGATTTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt                         <  1:251478/149‑1 (MQ=255)
                                                                                                                          gTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCg                       >  1:147352/1‑149 (MQ=255)
                                                                                                                           tttAAACTGGCGGTTGAATTGTTTTCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTTTCCTCATTCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGGAATAAATCGAg                     >  2:813704/1‑150 (MQ=255)
                                                                                                                            ttaaaccgggCGGTTGATTTTTTTTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                     <  1:337078/142‑1 (MQ=255)
                                                                                                                               aaCTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                >  1:733546/1‑151 (MQ=255)
                                                                                                                               aaCTGGCGGTTGAATTGTTATCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                >  2:745516/1‑151 (MQ=255)
                                                                                                                               aaCGGGGGGTGGAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                <  1:645295/151‑1 (MQ=255)
                                                                                                                                 cTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTcc              >  1:89615/1‑151 (MQ=255)
                                                                                                                                    gggggtttGAATTGTTGTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCt             <  2:796013/145‑1 (MQ=255)
                                                                                                                                     gggggTTGAATTTTTTGTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                <  1:104269/145‑1 (MQ=255)
                                                                                                                                          ttGATTTTTTGTTTAAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt        <  1:273396/150‑1 (MQ=255)
                                                                                                                                          ttGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt        >  1:1027534/1‑150 (MQ=255)
                                                                                                                                             aaTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct     <  1:339067/150‑1 (MQ=255)
                                                                                                                                             aaTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctg    >  2:890327/1‑151 (MQ=255)
                                                                                                                                             aaTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct     >  2:225047/1‑150 (MQ=255)
                                                                                                                                              aTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc   >  2:1096927/1‑151 (MQ=255)
                                                                                                                                                ttgttgTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgcc  >  2:1143628/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                       
ATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCC  >  NC_000913/2151543‑2151834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: