Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,678,0390TG49.1% 71.0 / 10.6 54N108T (AAC→ACC) yphEputative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (6/21);  new base G (26/0);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 1.14e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC  >  NC_000913/2677892‑2678189
                                                                                                                                                    |                                                                                                                                                      
aGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGtttt                                                                                                                                                     <  2:502983/151‑1 (MQ=255)
    gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCg                                                                                                                                                  <  1:664288/150‑1 (MQ=255)
       tttGCGCCGGTCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAGCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCa                                                                                                                                               <  2:540472/150‑1 (MQ=255)
                ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGGCAGAGGGTTTTCGCCCCTGTCAGccc                                                                                                                                     >  1:964307/1‑151 (MQ=255)
                 gCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCt                                                                                                                                    <  1:425477/151‑1 (MQ=255)
                     aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGGCCCAGGCCGAGGATTTTCCCCACCGGCAGCCCTTcc                                                                                                                                 >  2:752641/1‑150 (MQ=255)
                          tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAg                                                                                                                            <  1:1059110/150‑1 (MQ=255)
                           gAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTCCGCCACTCTCAGCCCCTcccc                                                                                                                               >  1:1232036/1‑144 (MQ=255)
                            aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACACAGGGGGAGGGTTTTCCCCCCCGTCAGCCCCTccacc                                                                                                                              >  1:755617/1‑146 (MQ=255)
                            aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGAc                                                                                                                          <  1:247793/150‑1 (MQ=255)
                             aaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTACTCAATCATGCCGTTGCGGCGGCGGCACAGCCCCAGGGCGAGGGTTTTCGCCTCCGTCAGGCCTTcccc                                                                                                                               >  2:1076365/1‑142 (MQ=255)
                              aCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACAGAAGCAGAAGGTTTTCGCCCCTGTCAGCCCCTCCCCCACACtt                                                                                                                        >  1:1172254/1‑150 (MQ=255)
                               cAAGTTGTTCAGGCCTAACGTCGACGCCCAGCGCCTGTAAGCAACGTGGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACtt                                                                                                                        <  1:353128/149‑1 (MQ=255)
                                aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAAc                                                                                                                     <  1:1112186/151‑1 (MQ=255)
                                 aGTTGTTCGGGTCTACCGTCAACGCCCAGGGCCTGTAAGCACCGTTGGGCATCCTGCGCGATTTGCAGGTATGCAATCATGCCGGTGCGGCGGGGCCACTGACCGAGGAAGAGGTGTTCCGCCGCTGTCAGCCCTTCCACCAGGCtt                                                                                                                        <  2:188564/147‑1 (MQ=255)
                                        cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCACTGTTAGCCCCTCCACCAGACTTAAATCCTGAt                                                                                                              >  1:445354/1‑150 (MQ=255)
                                         aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCGGAGGGTTTTCTCCCCCGTCATCCCCTCCACCCCACTTAACCCCTCATa                                                                                                             >  2:1240584/1‑150 (MQ=255)
                                               aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCCCTGTCACCCCCTCCACCCAAACTAACTCCCCATaaaac                                                                                                         >  2:208484/1‑146 (MQ=255)
                                                aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGTGGCCATGACCGCGGGCGGGGGTTTTCGTCACTCGCCGGCCTTCCCCCACACTTAATTCCCTATAAAAcgc                                                                                                       >  2:981158/1‑149 (MQ=255)
                                                aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCCGCCGCGGCAGAGGGTGTTCCCCCCTGTCACCCCCTTCCCCACACATTACCCCTAATaaaaccc                                                                                                       >  2:1178297/1‑145 (MQ=255)
                                                        gCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGGAGAGGGTTTCCTCCACCGTCAACCCCTCCCCCCGACTTAACTCCTGATAAAACACGCGCCacccc                                                                                             >  2:643572/1‑151 (MQ=255)
                                                          ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGGGGGTTTTCTCCACTGTCAACCCTTCCACCAGACTTAACTCCCGATAAAACCCGCGCAccccc                                                                                             >  2:447664/1‑149 (MQ=255)
                                                          ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg                                                                                           <  2:587700/151‑1 (MQ=255)
                                                          ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg                                                                                           <  2:215285/151‑1 (MQ=255)
                                                          ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg                                                                                           <  1:1139409/151‑1 (MQ=255)
                                                               gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGGAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCCGACTTAACTCCTGATAAAACCCGCGCAccccccgct                                                                                         >  1:720382/1‑144 (MQ=255)
                                                               gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTTCGCCACTGTCAACCCTTCCCCCCGACCTTACTCCTGATAAAACCCGCGCAccccc                                                                                             >  1:1250563/1‑144 (MQ=255)
                                                                ccTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCCAGGGAGAGGGTTTTCGCCCCCGTCAGCCCTTTCCCCCGCCTTAAATCCCTAGAAAACGCCCGCCCCCCCCGTTCCGc                                                                                     >  2:1199409/1‑151 (MQ=255)
                                                                 cTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCa                                                                                       <  2:362486/148‑1 (MQ=255)
                                                                   gTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCGCGGCGGGGGGGTTTCGCCCCTGCTAGCCCCTTCACCCGACGTTACCCCCGATAAAACACCCGCAccccc                                                                                             >  2:708982/1‑140 (MQ=255)
                                                                    tAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCGCCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCCGACTTAACTCCTTGTAAAACGCCCGCCCCCCCCGTTc                                                                                        >  2:1051918/1‑144 (MQ=255)
                                                                     acgcacctttGGGCATCCGGCGCCATTTACAGGTATCCAACCATGCCGTAGCGGCGGCGCCACTGCCAGAGGCAGGGGTTTTCCGCCCCTGTCAGCCCTTCCACCAAACTTAACTCCTGATAAACCGCGCGCCCCCCCATTTCAGCGGCa                                                                                 <  2:428341/142‑1 (MQ=255)
                                                                                    tCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCACACCTAACTCCCGATAAACCCCGCGCCCCCCCCCGTCAGCGGCACGGCGAGTCAGCg                                                                    >  1:1002811/1‑148 (MQ=255)
                                                                                       tGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCTACCGAGGGCGAGGGTTTTCGCCACCGCTAGCCCTTCCACCAGACTTAACTCCTGTTAAAACGCTCGCACCCCCCGGTCAGCCGCACGGCGGGGCAGCGTa                                                                  >  1:1034121/1‑147 (MQ=255)
                                                                                           ccATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGAACGAGGCCGAGGGTTTCCGCCCCTGTCAGCCCCTCCCCCAGACCTAAATCCTTATAAAACTCGCGCACCCCCCATTCAACGGGACGGCGAGGCAGCGTAGcct                                                              >  2:470769/1‑145 (MQ=255)
                                                                                             aTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGGAGAGGGTTTTCGCCCCCGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCCCTTCAGCGGCACGGCGAGTCAGCGGAGCTTCGTccc                                                        >  1:130751/1‑149 (MQ=255)
                                                                                                tGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCGGAGGGTTTTTCCCACCGTCAGCCCCTCCCCCAGACTTTACTCCTGATAAAACGCGCGCCCCCCCAGTTCAGCGGCACCGCGAGGCAGCGCAGGCTCGTCAcc                                                       >  2:1134076/1‑149 (MQ=255)
                                                                                                   aGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGACCGAGGCAGAAGGTTTCCTCCCCTGTCGGGCCTTTCACCAAACTTTAAACCTTAAAAACCCCGAGCACCCCCAGGTCAACGGCACGGGGGGTCAGCGTAGCATCGTCAcc                                                       >  1:505948/1‑146 (MQ=255)
                                                                                                     gTAATCAATCATGCCGTTGGGGCGGGGCCTCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGt                                                <  2:1058476/151‑1 (MQ=255)
                                                                                                       aatcaatcATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTc                                               <  1:133856/150‑1 (MQ=255)
                                                                                                        atcaatcaTGCCGTTGCGGCGGGGGCCCTGACCGAGGCAGAGGGTTTTCCCCACTGTCAGCCCCTCCCCCAGACTTTAATCCCGATAAAACCCGCGCACCCCCCGTTCAGCGGCACGGCGAGGCCGCGTAGCTTCGTCACCTTCCAATccc                                             >  1:1114145/1‑149 (MQ=255)
                                                                                                              caTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGtctc                                        <  1:1049408/150‑1 (MQ=255)
                                                                                                              caTGCCGTTGCGGCGGGGCCACTGAACGCGGGAGGGGGTTTTCGCCACTGTCAGCCCCTTCACCAGACTTAAATCCTGATAAAACCCGCGCCCCCCCCGATCAGCGGCACAGCGGGGCAGCGGAGCATCCTTACCTTCCCGTcgcg                                            >  2:619868/1‑146 (MQ=255)
                                                                                                               aTGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCCGTCCGCCCCTCCACCCGACTTTAATCCTGATAAAAAGCGCCGACCCCCCCTTCCGCGGCCCGGGGAGGCAGCGGAGGTTCGTCACCCTcc                                                   >  2:637109/1‑138 (MQ=255)
                                                                                                                        cggcggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATcc                                >  1:1009495/1‑148 (MQ=255)
                                                                                                                           cggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat                          >  1:447375/1‑151 (MQ=255)
                                                                                                                            ggggCCCCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCCCCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc                         <  1:915942/151‑1 (MQ=255)
                                                                                                                            ggggCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc                         >  1:1227832/1‑151 (MQ=255)
                                                                                                                                 ccACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCAcc                      >  1:113268/1‑150 (MQ=255)
                                                                                                                                           aGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGc           <  1:1199492/151‑1 (MQ=255)
                                                                                                                                           aGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGc           >  2:547288/1‑151 (MQ=255)
                                                                                                                                             gCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCg          >  1:449304/1‑150 (MQ=255)
                                                                                                                                             gCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGt         <  1:1015631/151‑1 (MQ=255)
                                                                                                                                                  ggTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt    <  2:947689/151‑1 (MQ=255)
                                                                                                                                                   gTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTg   <  1:981112/151‑1 (MQ=255)
                                                                                                                                                    ttttCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGc  >  2:248352/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC  >  NC_000913/2677892‑2678189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: