| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,678,043 | 0 | C | T | 41.1% | 59.7 / 25.6 | 56 | E107K (GAA→AAA) | yphE | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (11/22); new base T (23/0); total (34/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC > NC_000913/2677896‑2678189 | gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCg < 1:664288/150‑1 (MQ=255) tttGCGCCGGTCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAGCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCa < 2:540472/150‑1 (MQ=255) ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCCAGGCAGAGGGTTTTCGCCCCTGTCAGccc > 1:964307/1‑151 (MQ=255) gCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCt < 1:425477/151‑1 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGGCCCAGGCCGAGGATTTTCCCCACCGGCAGCCCTTcc > 2:752641/1‑150 (MQ=255) tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAg < 1:1059110/150‑1 (MQ=255) gAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGGTTTCCGCCACTCTCAGCCCCTcccc > 1:1232036/1‑144 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGGCCCTGACACAGGGGGAGGGTTTTCCCCCCCGTCAGCCCCTccacc > 1:755617/1‑146 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGAc < 1:247793/150‑1 (MQ=255) aaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTACTCAATCATGCCGTTGCGGCGG‑CGGCACAGCCCCAGGGCGAGGGTTTTCGCCTCCGTCAGGCCTTcccc > 2:1076365/1‑142 (MQ=255) aCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACCGACAGAAGCAGAAGGTTTTCGCCCCTGTCAGCCCCTCCCCCACACtt > 1:1172254/1‑150 (MQ=255) cAAGTTGTTCAGGCCTAACGTCGACGCCCAGCGCCTGTAAGCAACGTGGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACtt < 1:353128/149‑1 (MQ=255) aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAAc < 1:1112186/151‑1 (MQ=255) aGTTGTTCGGGTCTACCGTCAACGCCCAGGGCCTGTAAGCACCGTTGGGCATCCTGCGCGATTTGCAGGTATGCAATCATGCCGGTGCGGCGG‑GGCCACTGACCGAGGAAGAGGTGTTCCGCCGCTGTCAGCCCTTCCACCAGGCtt < 2:188564/147‑1 (MQ=255) cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGGTTTTCGCCACTGTTAGCCCCTCCACCAGACTTAAATCCTGAt > 1:445354/1‑150 (MQ=255) aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACCGACCGAGGCGGAGGGTTTTCTCCCCCGTCATCCCCTCCACCCCACTTAACCCCTCATa > 2:1240584/1‑150 (MQ=255) aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGGTTTTCGCCCCTGTCACCCCCTCCACCCAAACTAACTCCCCATaaaac > 2:208484/1‑146 (MQ=255) aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGTGGCCA‑TGACCGCGGGCGGGGGTTTTCGTCACTCGCCGGCCTTCCCCCACACTTAATTCCCTATAAAAcgc > 2:981158/1‑149 (MQ=255) aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGGCACCCGCCGCGGCAGAGGGTGTTCCCCCCTGTCACCCCCTTCCCCACACATTACCCCTAATaaaaccc > 2:1178297/1‑145 (MQ=255) gCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGGAGAGGGTTTCCTCCACCGTCAACCCCTCCCCCCGACTTAACTCCTGATAAAACACGCGCCacccc > 2:643572/1‑151 (MQ=255) ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGGGGGTTTTCTCCACTGTCAACCCTTCCACCAGACTTAACTCCCGATAAAACCCGCGCAccccc > 2:447664/1‑149 (MQ=255) ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg < 2:587700/151‑1 (MQ=255) ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg < 1:1139409/151‑1 (MQ=255) ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg < 2:215285/151‑1 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGGAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCCGACTTAACTCCTGATAAAACCCGCGCAccccccgct > 1:720382/1‑144 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACCGACCCAGGCAGAGGGTTTTCGCCACTGTCAACCCTTCCCCCCGACCTTACTCCTGATAAAACCCGCGCAccccc > 1:1250563/1‑144 (MQ=255) ccTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGGCCCTGACCCAGGGAGAGGGTTTTCGCCCCCGTCAGCCCTTTCCCCCGCCTTAAATCCCTAGAAAACGCCCGCCCCCCCCGTTCCGc > 2:1199409/1‑151 (MQ=255) cTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCa < 2:362486/148‑1 (MQ=255) gTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGGCCCTGACCGCGGCGGGGGGGTTTCGCCCCTGCTAGCCCCTTCACCCGACGTTACCCCCGATAAAACACCCGCAccccc > 2:708982/1‑140 (MQ=255) tAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCCCCCGCCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCCGACTTAACTCCTTGTAAAACGCCCGCCCCCCCCGTTc > 2:1051918/1‑144 (MQ=255) acgcacctttGGGCATCCGGCGCCATTTACAGGTATCCAACCATGCCGTAGCGGCGG‑CGCCACTGCCAGAGGCAGGGGTTTTCCGCCCCTGTCAGCCCTTCCACCAAACTTAACTCCTGATAAACCGCGCGCCCCCCCATTTCAGCGGCa < 2:428341/142‑1 (MQ=255) tCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCCCCGACCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCACACCTAACTCCCGATAAACCCCGCGCCCCCCCCCGTCAGCGGCACGGCGAGTCAGCg > 1:1002811/1‑148 (MQ=255) tGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCCCCTACCGAGGGCGAGGGTTTTCGCCACCGCTAGCCCTTCCACCAGACTTAACTCCTGTTAAAACGCTCGCACCCCCCGGTCAGCCGCACGGCGGGGCAGCGTa > 1:1034121/1‑147 (MQ=255) ccATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACCGAACGAGGCCGAGGGTTTCCGCCCCTGTCAGCCCCTCCCCCAGACCTAAATCCTTATAAAACTCGCGCACCCCCCATTCAACGGGACGGCGAGGCAGCGTAGcct > 2:470769/1‑145 (MQ=255) aTTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGGAGAGGGTTTTCGCCCCCGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCCCTTCAGCGGCACGGCGAGTCAGCGGAGCTTCGTccc > 1:130751/1‑149 (MQ=255) tGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCGGAGGGTTTTTCCCACCGTCAGCCCCTCCCCCAGACTTTACTCCTGATAAAACGCGCGCCCCCCCAGTTCAGCGGCACCGCGAGGCAGCGCAGGCTCGTCAcc > 2:1134076/1‑149 (MQ=255) aGGTAATCAATCATGCCGTTGCGGCGG‑GGGCACTGACCGAGGCAGAAGGTTTCCTCCCCTGTCGGGCCTTTCACCAAACTTTAAACCTTAAAAACCCCGAGCACCCCCAGGTCAACGGCACGGGGGGTCAGCGTAGCATCGTCAcc > 1:505948/1‑146 (MQ=255) gTAATCAATCATGCCGTTGGGGCGG‑GGCCTCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGt < 2:1058476/151‑1 (MQ=255) aatcaatcATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTc < 1:133856/150‑1 (MQ=255) atcaatcaTGCCGTTGCGGCGG‑GGGCCCTGACCGAGGCAGAGGGTTTTCCCCACTGTCAGCCCCTCCCCCAGACTTTAATCCCGATAAAACCCGCGCACCCCCCGTTCAGCGGCACGGCGAGGCCGCGTAGCTTCGTCACCTTCCAATccc > 1:1114145/1‑149 (MQ=255) caTGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGtctc < 1:1049408/150‑1 (MQ=255) caTGCCGTTGCGGCGG‑GGCCACTGAACGCGGGAGGGGGTTTTCGCCACTGTCAGCCCCTTCACCAGACTTAAATCCTGATAAAACCCGCGCCCCCCCCGATCAGCGGCACAGCGGGGCAGCGGAGCATCCTTACCTTCCCGTcgcg > 2:619868/1‑146 (MQ=255) aTGCCGTTGCGGCGG‑GGCCACCGACCCAGGCAGAGGGTTTTCGCCCCCGTCCGCCCCTCCACCCGACTTTAATCCTGATAAAAAGCGCCGACCCCCCCTTCCGCGGCCCGGGGAGGCAGCGGAGGTTCGTCACCCTcc > 2:637109/1‑138 (MQ=255) cggcgg‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATcc > 1:1009495/1‑148 (MQ=255) cgg‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat > 1:447375/1‑151 (MQ=255) gg‑ggCCCCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCCCCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc < 1:915942/151‑1 (MQ=255) gg‑ggCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc > 1:1227832/1‑151 (MQ=255) ccACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCAcc > 1:113268/1‑150 (MQ=255) aGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGc < 1:1199492/151‑1 (MQ=255) aGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGc > 2:547288/1‑151 (MQ=255) gCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCg > 1:449304/1‑150 (MQ=255) gCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGt < 1:1015631/151‑1 (MQ=255) gggtttCCGCCACTGTCAGTCCTTCCACCAGACTTAGCTCCTGATAAACCGCGCGCACACCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt < 2:212637/148‑1 (MQ=255) ggTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt < 2:947689/151‑1 (MQ=255) gTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTg < 1:981112/151‑1 (MQ=255) ttttCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGc > 2:248352/1‑151 (MQ=255) | GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGG‑GGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGC > NC_000913/2677896‑2678189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |