Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	44.4%	30.7 / 33.6	45	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/19);  new base T (20/0);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 7.46e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583                                                                                                                                                   |                                                                                                                                                      gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                                   <  1:746361/151‑1 (MQ=255)     ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACaa                                                                                                                                                >  1:421006/1‑150 (MQ=255)        gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                            <  2:543133/151‑1 (MQ=255)             aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                       <  1:51318/151‑1 (MQ=255)               aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACt                                                                                                                                     <  1:1101925/151‑1 (MQ=255)                ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAATAaaaaaa                                                                                                                                       >  1:1069657/1‑148 (MQ=255)                ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAaaaaaaaaaaa                                                                                                                                      >  1:124783/1‑148 (MQ=255)                   ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAATCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑gg                                                                                                                                  <  2:657003/149‑1 (MQ=255)                     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAaaaaaaaaaaa                                                                                                                                      >  2:615326/1‑143 (MQ=255)                      ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTTATAAAAATACaaaaaatagcggt                                                                                                                               >  2:1065614/1‑142 (MQ=255)                           tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTAATTTTTTCAGAAAAAAAAAAAAAAT‑GGAGGTGcctat                                                                                                                        >  2:647658/1‑148 (MQ=255)                            gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCTTCACAAAAAAAAAAAAT‑gggggtgggct                                                                                                                         >  2:358518/1‑140 (MQ=255)                                tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCGACAaaaaaaaaaaatacaggttcca                                                                                                                           >  1:1168904/1‑132 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCAatgttg                                                                                                                     <  2:921936/148‑1 (MQ=255)                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAA‑GGGGGTGGCATTATGAggg                                                                                                                 >  1:1189684/1‑149 (MQ=255)                                             attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACCAAAAAAAAAAAT‑GGAGGTTCCATGATGAGGGaaaaaaaaaa                                                                                                       >  1:57069/1‑143 (MQ=255)                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                             <  1:62737/150‑1 (MQ=255)                                                          cgTTGATTCACGAACTGTTAATTACATAGTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGTGAAAGTAATAAGCAACAGGCatt                                                                                           <  2:825224/149‑3 (MQ=255)                                                           gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTCTCAACAAATAAAAAAACT‑GGAGTTGCCATGATGAGCGGAAGGAATAAACAAAAgg                                                                                               >  1:725377/1‑144 (MQ=255)                                                             tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                       <  1:549413/150‑1 (MQ=255)                                                               aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCCGACAAAAACAAAAAAT‑GGGGGTGGCATGAGGAGCGGAAGGAATAAAAAACAGGCGGGGAaaaa                                                                                     >  1:1160766/1‑150 (MQ=255)                                                                 tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                   <  1:63459/150‑1 (MQ=255)                                                                  cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                  <  2:806545/150‑1 (MQ=255)                                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                           <  2:1130603/149‑1 (MQ=255)                                                                            tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTGATTTTTTCACAAAAAAAACAAAACG‑GGGGTTGCTCTGCTGTGGGTAAATAAAAAGCAACAGGCAGTGGAAAATGTAACAAAGATTg                                                                       >  1:443470/1‑151 (MQ=255)                                                                               ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAACAAAAAAAAAAAAT‑GGAGGTTGCATTATGAGCGAAAGTAATAAACAAAAAAAAGAGAATAAAATGACAGAGAATGt                                                                      >  2:331520/1‑149 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAA‑GGAGTTGCCATGATGAGCGAAAAAAATAAACAACAAGCAGTGAATAAGTTGAAAGAGATTGTCg                                                                    >  1:760305/1‑150 (MQ=255)                                                                                    tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTAAACAAAAAAAAAAAATGGGGGTTGCC‑TGATGGGGGAGAGGAAAAAAAAACAGGCAGTGAAAAAAATGACAGAGAATGTTGCTaa                                                                >  2:727131/1‑150 (MQ=255)                                                                                     atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAC‑GGGGTTGGCCTGATGAGCGGAAGGAATAAACAAAAGGCAGGGAAAAAATTGACAGAGATTTTCGCTaa                                                                >  1:981439/1‑149 (MQ=255)                                                                                     atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                               <  1:972142/150‑1 (MQ=255)                                                                                       tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAAA‑GGAGGTGTCCTGATGAGCGGAAGTAATAAACAACAAGCAGTGGATAAGGTGACAGAGATTGTCGCTAACttt                                                            >  1:771824/1‑151 (MQ=255)                                                                                       tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGAAGTTTGTGGCGGTGAAAAATTCCTACCAAACAAAAAAAAA‑GCAGGTGTCCTGGCGAAAGGAAAAAATAAGCAAAAAGCAGCGAATAAATTGAAAGAGATTGGCGCTAAAttt                                                            >  2:880421/1‑151 (MQ=255)                                                                                         aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                          <  2:305275/151‑1 (MQ=255)                                                                                            gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGc                                                       <  1:884206/151‑1 (MQ=255)                                                                                              ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                      <  1:73379/150‑1 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAAA‑GGGGGTTCCATGATGAGGGAAAGGAAAAAACAAAAAGCAGGGAATAAAATGACAGAGATTTTCGCTAACTTTaacccc                                                      >  1:1040231/1‑142 (MQ=255)                                                                                                    aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAAATTTTCAAACAAAAACAAAAAAA‑GGAGGTTGCATGATGTGGGGAAGGAATAAACAAAAAAGAATGAATAAGTTTACAAAGATTGGTGGTTACATTTACGCCACGAttt                                               >  2:42984/1‑151 (MQ=255)                                                                                                          aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAAAAAAAAAT‑GGAGGTGCCCTGATGAGGGGAAGTAATAAGCAACAGGCAGAGAATAAATTGAAAGAGATTGTCGCTAAATTTAACGCCCTGATTTTTTccc                                         >  1:153108/1‑151 (MQ=255)                                                                                                                  aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAAT‑GGAGGTGCCATGATGAGGGGAAAGAAAAAACAAAAGGCAGGGAATAAATTGACAGAGATTGTTGCTAAATTTTCCGCCCTGATTTTTACCCCAATGc                                   >  1:857726/1‑149 (MQ=255)                                                                                                                           ggtggtTTGTGGCGGTGATTTTTTCCAGACAAAAAAAAAAGCT‑GGAGTTGCGAGGATGGGAGAAAGAAATAAGCAACAGGATGTGAATAAGTTGACAGAGTTTGTGTATAACTTTCCCGCCATGATTTCTACCCGAATGCCtgatg                             <  1:1199358/146‑1 (MQ=255)                                                                                                                               gTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGGCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                    <  1:945630/151‑1 (MQ=255)                                                                                                                                   gggggggTGAATTTTTCCAGACAAATACAAAAACG‑GGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  2:57068/144‑1 (MQ=255)                                                                                                                                             aTTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:704026/1‑149 (MQ=255)                                                                                                                                                  tCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:140524/1‑150 (MQ=255)                                                                                                                                                  tCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:1230167/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑