Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	41.2%	123.7 / 34.7	80	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/42);  new base G (33/0);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.57e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354139‑354436                                                                                                                                                      |                                                                                                                                                     cTGGCGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                                      <  2:54184/150‑1 (MQ=255)         tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATg                                                                                                                                              >  1:885568/1‑150 (MQ=255)          ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc                                                                                                                                             <  1:99525/150‑1 (MQ=255)           cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGGAAGAt                                                                                                                                               >  2:888947/1‑147 (MQ=255)             tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGATGGGGGGGAAGAGGGGCt                                                                                                                                          >  1:936298/1‑150 (MQ=255)              gACGCTGACGGTTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGa                                                                                                                                        <  2:785899/151‑1 (MQ=255)               acacTGACGGATATCACGTTATTATCGGGCCCACGGACGATGTAATTAACGTTGCCGGGGATCGGCTGGGTACGCGAGAGATTGAAGAGAGTATGTCCAGCTCTCCGGGCGTTGCCGAAGTGGCTGTGGTTGGGGTGAAAGATGAGCTGag                                                                                                                                       <  2:85560/148‑2 (MQ=255)               aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  1:819366/151‑1 (MQ=255)                      cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                                   >  1:29545/1‑148 (MQ=255)                         ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGGTGGGGGGAAGGTGCGCCGTGAGGGCAggt                                                                                                                              >  2:81399/1‑150 (MQ=255)                            tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAgggggcg                                                                                                                          >  1:259671/1‑151 (MQ=255)                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                          <  1:905755/150‑1 (MQ=255)                              aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGGGCTGAAAGGGGAgggggggg                                                                                                                         >  1:359494/1‑150 (MQ=255)                               cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                        <  1:453637/150‑1 (MQ=255)                               cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCGGGAAggggcgggggggggg                                                                                                                       >  2:250240/1‑139 (MQ=255)                                tttATTCTCGGGCGCAATGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCCGGAAggggggggggccgggg                                                                                                                      >  1:1156181/1‑138 (MQ=255)                                 ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtgg                                                                                                                      <  2:677259/150‑1 (MQ=255)                                 ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:937660/149‑1 (MQ=255)                                 ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:879786/149‑1 (MQ=255)                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                                 <  2:122814/148‑1 (MQ=255)                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                               <  2:218534/150‑1 (MQ=255)                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                              <  2:786090/151‑1 (MQ=255)                                           gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                           <  2:23051/151‑1 (MQ=255)                                            cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGTGGCGGGGGCGGTTGTCAtt                                                                                                           >  1:887027/1‑150 (MQ=255)                                             gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCAtt                                                                                                           >  1:567710/1‑149 (MQ=255)                                                cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGTGCCGTAAAGGCAGGGGGGGGGGGGgtgtgtgttt                                                                                                           >  1:827797/1‑142 (MQ=255)                                                 tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCCGGAAGGGGGGGGGGGGGGGGGGGTTGTGATTTCgga                                                                                                      >  2:467258/1‑148 (MQ=255)                                                  gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                      <  1:309712/149‑1 (MQ=255)                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGaa                                                                                                      >  1:708872/1‑148 (MQ=255)                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGaa                                                                                                      >  1:840654/1‑148 (MQ=255)                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAGGGGGGGGGGGGGGTTTTCCTTTCGaa                                                                                                      >  1:245190/1‑148 (MQ=255)                                                    cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGGTGAAAGGGGAGGGGGGGGGGGGGGTTTTCATTCCGaaa                                                                                                     >  1:319153/1‑148 (MQ=255)                                                    cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGAAAAATGCGCTGAAAGGGGAGGGGGCGGGGGGGCTTGTCCTTTCGaaa                                                                                                     >  1:617835/1‑148 (MQ=255)                                                       tctAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGGGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGTGTGAAAGATGCGCTGAAAGGGCCGGTGGCGTTGGCGTTTGTCATTccta                                                                                                       <  2:294466/141‑3 (MQ=255)                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                               <  1:150145/150‑1 (MQ=255)                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                              <  1:413786/151‑1 (MQ=255)                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGGAAAGGGAGGGGGCGGGGGGGGTTTTTATTTCGAAAGAGAGCg                                                                                              >  1:490034/1‑149 (MQ=255)                                                            ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGTGGAAGAAGCGCGGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTTCGTAAAAGAGCggc                                                                                            >  2:1116582/1‑147 (MQ=255)                                                                    gCCGGGGATGGGCTGGCGACGTGCGAGATTGCAGAGGGTATCTCCAGCGGTTCGGCGGTTGCCTAAGTGGCGGTGGTTGGGGTGAAAGAGGCGCTGAAAGAGCAGGGTGCGGCTGGGTGTGTCACTCCGAATGACGGCGACa                                                                                           <  2:274169/142‑1 (MQ=255)                                                                    gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  2:1192981/151‑1 (MQ=255)                                                                    gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  1:1151499/151‑1 (MQ=255)                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                                 <  1:663032/151‑1 (MQ=255)                                                                      cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                <  1:259233/151‑1 (MQ=255)                                                                       gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGAAAGGGCCGGGGGCGGGGGGGGTTGTTCTTTCGAAAGAGAACGACAGTCTgg                                                                                     >  1:657105/1‑145 (MQ=255)                                                                          cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGGAAAGGGAGGGGGGGGGGGGGGGTGTCATTTCGGAAGAGAGCGACAGACTGGaaaaacggg                                                                            >  2:888381/1‑144 (MQ=255)                                                                             cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                         <  2:1232425/151‑1 (MQ=255)                                                                                cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGGGACAGTCTGGAAGATCGTGATGTgg                                                                      >  1:288826/1‑151 (MQ=255)                                                                                  ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGGGGTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGGCAGGGTGGGAGATCGGGATGGGgcg                                                                    >  2:90024/1‑151 (MQ=255)                                                                                      aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                                <  1:1041520/151‑1 (MQ=255)                                                                                        gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGGGGGGCACTc                                                               >  1:394107/1‑150 (MQ=255)                                                                                        gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCCGAAAGGGGAGGGGGCGGGGGCGGTTGTTATTTCGAAAGAGAGCGACAGTCTGGAAAATCGTGGTGTGGCGCACTc                                                               >  1:568746/1‑150 (MQ=255)                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                        <  1:743148/151‑1 (MQ=255)                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCGGAAAAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCACTCGcca                                                           >  1:911325/1‑146 (MQ=255)                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACCCGc                                                             >  1:535139/1‑145 (MQ=255)                                                                                                 tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCaa                                                           >  1:16451/1‑145 (MQ=255)                                                                                                     gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                     <  1:359459/147‑1 (MQ=255)                                                                                                     gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTGGGGGTGAAAAATGCGCTGAAAGGGGAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGGTAGACTGGGAGATCGGGGTGTGGGGCCATCGCAAGAGaa                                                      >  2:359447/1‑146 (MQ=255)                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                               <  1:888992/151‑1 (MQ=255)                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                               <  1:97013/151‑1 (MQ=255)                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                               <  1:81401/151‑1 (MQ=255)                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                               <  1:775041/151‑1 (MQ=255)                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCTGGAAGGGGAGGTGGCGGGGGCGTTTGTCATTCCGGAAAAGAGCGGCCGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAgg                                                    >  1:522605/1‑146 (MQ=255)                                                                                                            tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGCGGTTTTCATTCCGAAAGAGAGCGGCAGGCTGGAAGATCGTGGTGTGGCGCACCCGCAAGAGAAGGCGATTATgg                                           >  2:362232/1‑150 (MQ=255)                                                                                                                  gCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAGATGCGCTGAAAGGGGGGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAAAGGGGTGTGGCGCACCCGCACGAGAAGGCGATTATGGGGGtgg                                     >  2:1015736/1‑150 (MQ=255)                                                                                                                     aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGCGGGGGGGGGGGCGGTTGTCGTTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCtggggg                                  >  1:1128771/1‑150 (MQ=255)                                                                                                                        cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAg                              <  1:630963/151‑1 (MQ=255)                                                                                                                        cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAg                              <  1:1032419/151‑1 (MQ=255)                                                                                                                             gTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGCGGGGGGGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCCAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                            >  1:1259344/1‑148 (MQ=255)                                                                                                                              ttGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAGGTGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGGCTGGCTGGGAGATAGTGGTGTGGCGCACTCGCAAGAGAAGGCGGATACGGCGCtggt                                    >  2:459160/1‑139 (MQ=255)                                                                                                                                gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                       <  2:287144/150‑1 (MQ=255)                                                                                                                                gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                      <  2:853181/151‑1 (MQ=255)                                                                                                                                 ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                     <  2:985598/151‑1 (MQ=255)                                                                                                                                 ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                     <  2:129178/151‑1 (MQ=255)                                                                                                                                       tGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCCGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGCTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGAGGACAGCCAGATTGGCAACtt               >  2:461755/1‑151 (MQ=255)                                                                                                                                        ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt              <  2:1184117/151‑1 (MQ=255)                                                                                                                                         gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg             <  1:57271/151‑1 (MQ=255)                                                                                                                                           ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc           <  1:989654/151‑1 (MQ=255)                                                                                                                                                 tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg     >  1:1078185/1‑151 (MQ=255)                                                                                                                                                   gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc   <  2:708838/151‑1 (MQ=255)                                                                                                                                                   gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc   <  2:883078/151‑1 (MQ=255)                                                                                                                                                    ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  <  2:673206/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354139‑354436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑