Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,516	0	C	A	41.5%	66.0 / 27.7	54	Q295K (CAA→AAA)	thiL	thiamine monophosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (27/4);  new base A (0/22);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 3.23e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGC  >  NC_000913/436366‑436662                                                                                                                                                             |                                                                                                                                                     cTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGc                                                                                                                                                      >  1:418175/1‑151 (MQ=255)    tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGAAACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑aa                                                                                                                                                   >  2:220474/1‑150 (MQ=255)       cGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑Ga                                                                                                                                              <  2:28845/151‑1 (MQ=255)         ccATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GAcc                                                                                                                                            <  2:53046/151‑1 (MQ=255)             gTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GAccgcc                                                                                                                                         <  1:813288/150‑1 (MQ=255)                    cGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCg                                                                                                                                  >  2:740228/1‑150 (MQ=255)                     ggAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGa                                                                                                                                 >  1:654476/1‑150 (MQ=255)                     ggAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑GACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGa                                                                                                                                 >  2:692103/1‑150 (MQ=255)                           ggggcggcggggggCGCTCTCGGGGGGAGAAGATTGCGGTTTGTTTTTCCCTGTCCCGA‑AACTGACCCGGGGCGCGTT‑GGGGGTGCCTCCCGCCCCCTG‑GGGGG‑TCCT‑T‑TTTACCTTTTGGGGAA‑AAAT‑GACCGCCGATATCGAAGGGCtt                                                                                                                          <  2:1180320/140‑1 (MQ=255)                            gcgctgcgctGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCttt                                                                                                                         >  1:402729/1‑151 (MQ=255)                                  cgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGtttt                                                                                                                    >  1:979128/1‑150 (MQ=255)                                  cgcggGGCGCCCTCGGGGGGTGAAGATTCGGGTTTGTTTTTCCCTGTGCCGG‑AACTGACCCGGGGCGCGCT‑GGATGTGGCTCCCGGACCCTG‑GGGCG‑TCCT‑T‑TTTTCTTGTACCGGAA‑AATT‑GACCGCCGATATCGAAGGGCTTTGtttt                                                                                                                    <  2:1106924/150‑1 (MQ=255)                                   ggtgGGCCTTCTCGGGCGGTGAAGTTTCCGATTTGTGTTTCCCTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCCCTCGGCCACCT‑GGGGG‑TCCC‑T‑TTTCCTTTTATGGGGA‑AAAA‑GACCGCCGATATCGAAGGGCTTTGTTTTAt                                                                                                                  <  1:817194/149‑1 (MQ=255)                                      gggcctctgcggggggTGAAGATTAGGGTTTTTGTTTCCCTGGGCCGG‑AACTGAACGGTGGCGCGTT‑GGTTGTGCCCCGCGCAC‑CCG‑GGGGG‑TTCCTT‑TTTCCCTTTAGCGGAA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTAtt                                                                                                                 <  2:1078737/135‑1 (MQ=255)                                      gggCGCTCTGTGGCGGGAAAGTTTACGTGTTTTTTTTCTGTGTGCGGG‑AACTGACCGGGGGCGCGCT‑GGGTGTGGCTCTCGGACCCTG‑GGGCG‑TCCT‑G‑TTTCCCTGTGGCGGGA‑AAAT‑GACCGCCGATATCGAAGGGCttt                                                                                                                         <  2:254210/141‑1 (MQ=255)                                         cgcTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTg                                                                                                             >  1:1168517/1‑150 (MQ=255)                                               ctGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc                                                                                                        >  1:804978/1‑149 (MQ=255)                                                 ggggttGAAGATTCGGATTTGTTTTGTATGGTCCCGG‑AACTGCTCCGGGGCGCGCG‑GGTTGTCTCCTTCGG‑CGACG‑GGGCT‑TTCC‑GTTTTCCCTTTGTCGGAA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGaa                                                                                                     <  2:784792/145‑1 (MQ=255)                                                 ggCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGACCCGGGGCGCGTT‑GGTTGTGGCTCTCGGACACTG‑GGGCG‑TACC‑G‑TTTCCTTTTATGGGGA‑AAAT‑GCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc                                                                                                        <  1:1032022/147‑1 (MQ=255)                                                 ggCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGaa                                                                                                     >  2:203604/1‑150 (MQ=255)                                                    ggtgaagtttcgggtttGTGTTTCACTGTGCGGG‑AACTGACCGGGGGCGCGTG‑GGATGGGTCTCTCGGACCCCG‑GGGCG‑TCCT‑T‑TTTCCTTGTAGCGGGA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTg                                                                                                 <  2:815475/136‑1 (MQ=255)                                                    ggTAAAAATTTCGATTTGTTTTTCACTGTGCGAG‑AACTGACCTGCGGCCCGTG‑GGGTGGGGCTCGCGGCCACGT‑GGGCG‑TACT‑T‑TTTACCTGTACCGGGA‑AAGT‑CACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCt                                                                                                  <  2:349134/150‑1 (MQ=255)                                                         aGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTAc                                                                                             >  2:791576/1‑150 (MQ=255)                                                             taggAGTTTTTTTTCCCTGGGCCGG‑AACTGACCGGGGGCGCGTT‑GGTTGTGGCTCTGGGACCCCG‑GGGCG‑TCCC‑T‑TTTCCTTGTATCGGGA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTa                                                                                         <  1:1050362/147‑1 (MQ=255)                                                             tacgttttGTGTTTCCCTGTGCCGG‑AACGGACCGGGGGCGCGTT‑GGTGGTGCCTCCGGGACACCT‑GGGCT‑GCCC‑T‑TTTCCCTTTGCGGGAA‑AAAG‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTa                                                                                         <  2:435075/144‑1 (MQ=255)                                                             tACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGCCACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                          >  2:2904/1‑149 (MQ=255)                                                               cGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGAc                                                                                      >  2:140274/1‑151 (MQ=255)                                                                 ggttgttTTTCCTTGTGCCGA‑AACGGACCCGTGGCGCGGT‑GGGTGGGGCTC‑CGGGAACCTGGGGCG‑TCCC‑T‑TTTTCCTGTACCGGGA‑AAAG‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAg                                                                                        <  1:634064/146‑1 (MQ=255)                                                                    tgtgTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGa                                                                                  >  1:415923/1‑150 (MQ=255)                                                                     tttttttcccgggCCGGAAACTGACCCGGGGCGCGTT‑GGTTGTGCCTCTCGGCCCCCG‑GGGCG‑TCCC‑T‑TTTCCTTGTTTCGGGA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGa                                                                                  <  1:1086262/138‑1 (MQ=255)                                                                           ctcTGTGCGGG‑AAC‑GAACCGGGGGGCGGTGGGGTGTGCCTC‑CGGGCCCCT‑GGGGGTTCCC‑T‑TTTACCTGTATGGGAA‑AAAG‑GCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGata                                                                           <  2:189651/148‑1 (MQ=255)                                                                           cACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGatat                                                                          >  2:788386/1‑151 (MQ=255)                                                                                tgCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGAcc                                                                      >  1:380946/1‑150 (MQ=255)                                                                                     gaaaCTGACCCTTGGCGGGTT‑GGTTGTGTCTCCGGAACCCCT‑GGGCG‑T‑CC‑C‑TTTTCCTTTTCCGGGG‑AAATGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtt                                                                   <  1:396090/147‑1 (MQ=255)                                                                                       accggcccggTGGCGCGTT‑GGATGTGGCTCCCGGCCACCT‑GGGCG‑TACC‑T‑TTTCCCTGTATGGGGA‑AAAT‑GCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtttt                                                                 <  2:262218/140‑1 (MQ=255)                                                                                       aaCTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGc                                                               >  1:468079/1‑151 (MQ=255)                                                                                          tGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc                                                            >  2:200081/1‑151 (MQ=255)                                                                                           gAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                           >  2:261005/1‑151 (MQ=255)                                                                                               cgtgcggcgcT‑GGAGGTGCTCCTCGTACCCTG‑GGGCG‑TACT‑T‑TTTCCTTTTGTCGGAA‑AAAT‑AACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                           <  2:1222531/141‑1 (MQ=255)                                                                                                  ggCGGGTT‑GGGTGTGCCTCTCGTCCACCT‑GGGTT‑TCCC‑G‑TTTCCTTGTATCGGGA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc                                                            <  1:703850/143‑1 (MQ=255)                                                                                                      cgcT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGTCGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg                                                >  2:322051/1‑151 (MQ=255)                                                                                                       ggt‑GGATGTGTCTCTCGGCCCCTG‑GGGCG‑TCCT‑T‑TTTCCTTGTAGCGGGA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg                                                <  2:243903/148‑1 (MQ=255)                                                                                                       gcT‑GGATGTGGCTATCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg                                                >  2:651959/1‑150 (MQ=255)                                                                                                         t‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcg                                             >  1:1188573/1‑151 (MQ=255)                                                                                                            gttggtgcctccGGACCCCTGGGGCG‑TCCC‑T‑TTTACCTTTA‑CGGGCAAAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTc                                              <  2:95360/138‑1 (MQ=255)                                                                                                                tgGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGaaa                                        >  2:937685/1‑150 (MQ=255)                                                                                                                 ggCTCTGGGCCCCCG‑GGGCG‑TCCC‑T‑TTTCCTTTTAGGGGGA‑AAAT‑GCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGaaaa                                       <  1:284147/150‑1 (MQ=255)                                                                                                                     ctcGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGc                                  >  1:851242/1‑151 (MQ=255)                                                                                                                              cgggggCG‑TCCC‑T‑TTTCCTTGTAGGGGAA‑AAAG‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGa                           <  1:1132430/147‑1 (MQ=255)                                                                                                                                              tttCCCTGTATCGGAA‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCg                <  1:920961/148‑1 (MQ=255)                                                                                                                                               ttACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGc            >  1:433743/1‑151 (MQ=255)                                                                                                                                                   cTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCt        >  1:1273077/1‑151 (MQ=255)                                                                                                                                                      tATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACt     >  1:637848/1‑151 (MQ=255)                                                                                                                                                         cGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGc  >  2:1178118/1‑151 (MQ=255)                                                                                                                                                          gggA‑AAAT‑GCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACtt    <  1:140276/148‑1 (MQ=255)                                                                                                                                                             |                                                                                                                                                     CTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGG‑AACTGAACCGTGGCGCGCT‑GGATGTGGCTCTCGGACACCT‑GGGCG‑TACC‑G‑TTTACCTGTATCGGGC‑AAAT‑GACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGC  >  NC_000913/436366‑436662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑