Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,377,173 A→C F83C (TTC→TGC)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,1730AC98.7% 259.4 / NA 75F83C (TTC→TGC) sspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (34/40);  minor base G (1/0);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTT  >  NC_000913/3377027‑3377319
                                                                                                                                                  |                                                                                                                                                  
gTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAAc                                                                                                                                                 <  1:1192009/150‑1 (MQ=255)
gTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACg                                                                                                                                                <  2:976884/151‑1 (MQ=255)
   gCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTc                                                                                                                                             <  2:345087/151‑1 (MQ=255)
     ttACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCa                                                                                                                                            >  2:491114/1‑150 (MQ=255)
       aCGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATcc                                                                                                                                         >  2:77236/1‑151 (MQ=255)
           gCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACTGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGat                                                                                                                                     <  1:141658/151‑1 (MQ=255)
             ggCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCCACGCTCATCCAGata                                                                                                                                    >  2:1134112/1‑150 (MQ=255)
             ggCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGatat                                                                                                                                   <  2:45950/151‑1 (MQ=255)
              gCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATAtt                                                                                                                                  <  1:362998/151‑1 (MQ=255)
                    gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCAt                                                                                                                              <  2:459512/149‑1 (MQ=255)
                    gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATaa                                                                                                                            <  1:994268/151‑1 (MQ=255)
                    gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATa                                                                                                                             <  1:131626/150‑1 (MQ=255)
                       tCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAat                                                                                                                           <  2:36463/149‑1 (MQ=255)
                         aGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAatgatg                                                                                                                       <  1:642789/151‑1 (MQ=255)
                             gCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCg                                                                                                                     >  1:123023/1‑149 (MQ=255)
                              cTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCg                                                                                                                     >  2:278118/1‑148 (MQ=255)
                                 aaCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGAtt                                                                                                                >  1:956922/1‑150 (MQ=255)
                                 aaCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTc                                                                                                               <  1:876332/151‑1 (MQ=255)
                                   ccGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTcc                                                                                                              <  2:775923/150‑1 (MQ=255)
                                       tgatgaTGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCCACGCTCCTCCAGATATTCCATCATGATGCggggtttc                                                                                                              >  2:752136/1‑139 (MQ=255)
                                         atgatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAggg                                                                                                       <  2:663049/151‑1 (MQ=255)
                                           gatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGt                                                                                                      <  2:643815/150‑1 (MQ=255)
                                            atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTc                                                                                                     >  2:359722/1‑150 (MQ=255)
                                            atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCa                                                                                                    <  2:422337/151‑1 (MQ=255)
                                              ggTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGc                                                                                                  <  1:90630/151‑1 (MQ=255)
                                                 gtTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAAAGCTCATCCAGATATTCCATAATGATGCGAGATTTCCACAGGGTCACCTc                                                                                                >  1:240290/1‑150 (MQ=255)
                                                  ttCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa                                                                                               >  1:49235/1‑150 (MQ=255)
                                                     atcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGAGGCGGATGCGGGCAACGGTAATCCAGATATTCCCTAATGATGCGAAATTCCCACCGggtcaggtcaag                                                                                             >  1:84995/1‑147 (MQ=255)
                                                          cGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccacc                                                                                      >  2:356585/1‑151 (MQ=255)
                                                          cGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAGATATTCCATAATGATGCGAGAGTCCCACAGGGACAGCTCACGATccacc                                                                                      >  2:371476/1‑151 (MQ=255)
                                                           gtgtACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccacca                                                                                     <  2:387041/151‑1 (MQ=255)
                                                             gtACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAg                                                                                    <  1:31113/150‑1 (MQ=255)
                                                               aCCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGGAACGCTCATCCCGATATTCCATAATGAAGCGAGATTCCCACAGGGTCCGCTCACGATCCACCCggg                                                                                  >  2:278283/1‑150 (MQ=255)
                                                               aCCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAggg                                                                                  <  2:466491/150‑1 (MQ=255)
                                                                 cAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCg                                                                               <  1:688368/151‑1 (MQ=255)
                                                                 cAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCg                                                                               >  1:145067/1‑151 (MQ=255)
                                                                 cAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCg                                                                               <  1:1115872/151‑1 (MQ=255)
                                                                     cTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGaa                                                                            >  2:1202442/1‑150 (MQ=255)
                                                                     cTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGaa                                                                            >  2:220990/1‑150 (MQ=255)
                                                                       ttttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAAc                                                                           <  1:342492/149‑1 (MQ=255)
                                                                           cgatgcgatgCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTg                                                                     <  1:1108330/151‑1 (MQ=255)
                                                                             atgcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCCACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGAt                                                                   >  1:947865/1‑151 (MQ=255)
                                                                               gcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGAtt                                                                  <  2:334029/150‑1 (MQ=255)
                                                                                cgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCg                                                                >  1:351178/1‑151 (MQ=255)
                                                                                 gatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCCCAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCgg                                                               >  2:135680/1‑151 (MQ=255)
                                                                                 gatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGGCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCgg                                                               >  2:1004061/1‑151 (MQ=255)
                                                                                  atgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCggg                                                              >  1:1078379/1‑151 (MQ=255)
                                                                                   tgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGTGAGATTCCCACAGGGGCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCggg                                                              >  2:385765/1‑150 (MQ=255)
                                                                                    gcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGt                                                             <  1:406162/150‑1 (MQ=255)
                                                                                      aTGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGa                                                          <  2:1041184/151‑1 (MQ=255)
                                                                                           cAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAgg                                                        >  2:481637/1‑148 (MQ=255)
                                                                                            aGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCa                                                     >  1:1027261/1‑150 (MQ=255)
                                                                                               cGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATc                                                  >  1:640391/1‑150 (MQ=255)
                                                                                                      cACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTg                                          <  2:551401/151‑1 (MQ=255)
                                                                                                       aCCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTg                                          <  1:460000/150‑1 (MQ=255)
                                                                                                        ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTg                                          >  1:187680/1‑149 (MQ=255)
                                                                                                         cgcgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAgg                                       >  2:172117/1‑151 (MQ=255)
                                                                                                         cgcgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGCAAGCTCTGATTCGGGTTGAGGTCAATCAGATTCTGAg                                        >  2:1465/1‑150 (MQ=255)
                                                                                                                 aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTg                                <  2:1042522/150‑1 (MQ=255)
                                                                                                                 aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTg                                <  1:233767/150‑1 (MQ=255)
                                                                                                                      gTAAACAGGCATCAGTGGCGGATGCGGACAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCttt                          <  1:766075/151‑1 (MQ=255)
                                                                                                                        aaaCAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCtttt                         >  1:862103/1‑150 (MQ=255)
                                                                                                                        aaaCAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTc                        <  2:84756/151‑1 (MQ=255)
                                                                                                                          aCAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                      <  2:332655/151‑1 (MQ=255)
                                                                                                                           cAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAc                     <  2:740891/151‑1 (MQ=255)
                                                                                                                              gCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACg                    >  2:1266407/1‑149 (MQ=255)
                                                                                                                                         ggATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg        >  2:1078660/1‑150 (MQ=255)
                                                                                                                                         ggATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg        >  2:1205228/1‑150 (MQ=255)
                                                                                                                                          gATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGaa      <  2:859824/151‑1 (MQ=255)
                                                                                                                                           aTGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGAGCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtctc         <  2:770997/147‑1 (MQ=255)
                                                                                                                                             gCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACt   <  1:1062870/151‑1 (MQ=255)
                                                                                                                                              cGGGCATCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCGCGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTGAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt  <  2:926362/151‑1 (MQ=255)
                                                                                                                                              cGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt  >  2:1026642/1‑151 (MQ=255)
                                                                                                                                              cGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt  <  1:37267/151‑1 (MQ=255)
                                                                                                                                               gggCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt  >  1:79457/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTT  >  NC_000913/3377027‑3377319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: