BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	44.2%	167.0 / 55.6	113	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base T (8/55); new base G (50/0); total (58/55)
Fisher's exact test for biased strand distribution p-value = 9.73e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354138‑354437

aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                                          <  1:971293/151‑1 (MQ=255)

   ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg                                                                                                                                                         <  2:1849494/149‑1 (MQ=255)

    gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                      <  2:214380/151‑1 (MQ=255)

          ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                                <  2:860778/151‑1 (MQ=255)

            gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCt                                                                                                                                              <  1:247896/151‑1 (MQ=255)

            gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCt                                                                                                                                              <  2:753549/151‑1 (MQ=255)

             tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑g                                                                                                                                            >  1:299552/1‑151 (MQ=255)

              gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑Ga                                                                                                                                           <  1:198860/151‑1 (MQ=255)

              gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGGGCt                                                                                                                                              >  2:878134/1‑149 (MQ=255)

              gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGaagggg                                                                                                                                               >  1:1630932/1‑143 (MQ=255)

              gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCT‑g                                                                                                                                            >  1:555310/1‑150 (MQ=255)

                   tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGGGGAAGAAGCGCC‑GAAAggg                                                                                                                                      >  1:1906369/1‑151 (MQ=255)

                    gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAggg                                                                                                                                      <  1:1586057/150‑1 (MQ=255)

                    gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGc                                                                                                                                     <  2:945116/151‑1 (MQ=255)

                     aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCT‑GAAAggg                                                                                                                                      >  2:1145517/1‑149 (MQ=255)

                      cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCT‑GGAAggg                                                                                                                                      >  1:717182/1‑148 (MQ=255)

                       ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCT‑GAAAGGGGAgg                                                                                                                                  >  2:210728/1‑151 (MQ=255)

                        gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAgg                                                                                                                                  <  2:1022516/150‑1 (MQ=255)

                         ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAgggg                                                                                                                                >  2:1737018/1‑149 (MQ=255)

                          taacACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtg                                                                                                                                <  2:493909/147‑1 (MQ=255)

                           aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggc                                                                                                                              <  1:1560455/151‑1 (MQ=255)

                           aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtgg                                                                                                                               <  1:12598/150‑1 (MQ=255)

                            tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCT‑GGAAgggggggggggg                                                                                                                             >  1:1140617/1‑142 (MQ=255)

                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcgg                                                                                                                            <  2:1238939/151‑1 (MQ=255)

                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcg                                                                                                                             <  2:1700212/150‑1 (MQ=255)

                                tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCT‑GGAAggggcggggggggggg                                                                                                                         >  1:1596664/1‑138 (MQ=255)

                                 ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcggtgg                                                                                                                         <  1:139441/150‑1 (MQ=255)

                                     tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                     <  1:1103301/150‑1 (MQ=255)

                                     tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGtt                                                                                                                     <  1:666884/150‑1 (MQ=255)

                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GGAAGGGGAGGTGGCGGGGGGGttt                                                                                                                    >  2:259646/1‑148 (MQ=255)

                                         gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCa                                                                                                                <  1:689473/151‑1 (MQ=255)

                                          ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑t                                                                                                              <  1:1896056/151‑1 (MQ=255)

                                           gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGGC‑GAAAGGGGAGGGGGGGGGGGGGGTTGtgctt                                                                                                              >  2:370885/1‑147 (MQ=255)

                                             gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTCA‑tt                                                                                                             >  2:21106/1‑149 (MQ=255)

                                                  gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑Gaa                                                                                                       <  1:257835/149‑1 (MQ=255)

                                                  gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑Gaa                                                                                                       <  2:1823995/149‑1 (MQ=255)

                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAaga                                                                                                    <  1:1881169/151‑1 (MQ=255)

                                                      aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAagagag                                                                                                 <  2:1065372/151‑1 (MQ=255)

                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGc                                                                                                <  2:700217/150‑1 (MQ=255)

                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCg                                                                                               <  1:1310639/151‑1 (MQ=255)

                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTTC‑TTCC‑GAAagaga                                                                                                  >  2:2023314/1‑148 (MQ=255)

                                                         tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGGT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTTA‑TTCC‑GAAaaagaggg                                                                                               >  1:904102/1‑148 (MQ=255)

                                                           aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GGAAGGGGAGGGGGCGGGGGGGGTTGTCA‑TTCC‑Ggaa                                                                                                      >  1:1590941/1‑141 (MQ=255)

                                                           aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCT‑GGAAGGGGAGGGGGCGGGGGCGGTTGTCA‑TTCC‑GAAagaggacg                                                                                               >  1:403511/1‑144 (MQ=255)

                                                              aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGt                                                                                          <  2:646175/150‑1 (MQ=255)

                                                              aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCT‑GAAAGGGGAGGTGGGGGGGGCGGTTGTCA‑TTCC‑CAAAAAGAGCGACAGt                                                                                          >  2:641948/1‑150 (MQ=255)

                                                               aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCT‑GAAAGGGGAGGGGGCGGGGGGGGTTGTCA‑TTTC‑GAAAGAGAGCGACAGTCt                                                                                        >  2:1555693/1‑151 (MQ=255)

                                                                   tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGa                                                                                     <  2:57526/150‑1 (MQ=255)

                                                                    gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGaa                                                                                    <  2:1810636/150‑1 (MQ=255)

                                                                        ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCg                                                                               <  1:1356218/151‑1 (MQ=255)

                                                                         gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGt                                                                              <  1:35158/151‑1 (MQ=255)

                                                                          cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTTTCA‑TTCC‑GAAAGAGAGGGACAATCTGGAAGATCg                                                                               >  2:1070743/1‑149 (MQ=255)

                                                                              ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCT‑GGAAGGGGAGGGGGGGGGGGGGGTTTTCA‑TTTC‑GGAAGAGAGGGGAAAGCTGGAAGAACGTg                                                                             >  1:224980/1‑147 (MQ=255)

                                                                                cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGGGCTGGAAAGGGC‑GGGGGCGGGGGGGGTTGTTA‑TTTC‑GAAAGAGAGGGAAAGTCTGGAAGGACGGGGTGTgg                                                                       >  1:1969955/1‑151 (MQ=255)

                                                                                  ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGAGCGTGATGTGgc                                                                      <  1:410498/150‑1 (MQ=255)

                                                                                     gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCT‑GGAAGGGGAGGGGGGGGGGGGGGTTGTCA‑TTCC‑GAAAGAGAGCGGCAGGCTGGGAGATCggggtgggg                                                                       >  1:661229/1‑138 (MQ=255)

                                                                                      aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGTGCT‑GGAAAGGCAGGGGGCGGGGGGGGTTGTCA‑TTCC‑TGAAGAGAGGGGCAGGCTGGAAGATGGTGATGTGGGGCa                                                                   >  2:1499154/1‑149 (MQ=255)

                                                                                         cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATTCGCT‑GGAAGGGCAGGGGGGGGTGGCGTTTGTCA‑TTTC‑GAAAGAGAGGGAAAGTCTGGAAGATCGGGATGTgggg                                                                     >  2:854693/1‑142 (MQ=255)

                                                                                            gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                           <  2:534342/151‑1 (MQ=255)

                                                                                            gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGGT‑GGAAGGGGAGGTGGCGGCGGCGTTTGGCA‑TTCC‑GCAAGAGAGCGCAACTTTGGGAGAACGTGATGGGGCGCACTCGCa                                                             >  2:1157592/1‑149 (MQ=255)

                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                         <  1:1882936/151‑1 (MQ=255)

                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCA‑TTCC‑GGAAGAGAGCGACAGTCTGGAAGAACGTGGTGGGGGGCACTCGCAag                                                           >  2:1105454/1‑149 (MQ=255)

                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCT‑GGAAGGGGCGGGGGGGGTGGGGGTTGTTA‑TTTC‑GGAAGAGAGGGACAGTCTGGGAGAACGTGATGTGgggcccccggaa                                                            >  1:1320813/1‑139 (MQ=255)

                                                                                                ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTGTCA‑TTCC‑GCAAGAGAGCGGCAGTCTGGGAAATCGTGATGGGGGGCACCCGCaa                                                            >  1:1822752/1‑146 (MQ=255)

                                                                                                 tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       <  2:2077807/150‑1 (MQ=255)

                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  2:1701986/150‑1 (MQ=255)

                                                                                                   aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                    <  2:445171/151‑1 (MQ=255)

                                                                                                   aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                    <  1:1145483/151‑1 (MQ=255)

                                                                                                    agagagTATCTCCAGCCATCCGGGCGTTTCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GGAAGGGGAGGGGGGGGGGGCGGTTGTCATTTCC‑GAAA‑AGAGGGAGAAGCTGGGAGAGCGGGGTGGGGGGCACCCGCAAGAGAAGGc                                                    >  1:956287/1‑150 (MQ=255)

                                                                                                    agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGGTGCGCT‑GAAAGGGCAGGTGGGGGTGGCGGTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGGAGATAGTGATGTGGCGCACTCGCAAGAGGAGGCg                                                   >  2:121285/1‑151 (MQ=255)

                                                                                                    agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCA‑TTTC‑CAAAAAGAGGGACAGTCTGGGAGACAGGGGTGGGGGGCACTCGCAAGAGAAgg                                                     >  2:1140966/1‑149 (MQ=255)

                                                                                                     gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                                  <  1:1226946/151‑1 (MQ=255)

                                                                                                     gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGAGAGAAGCGCT‑GTAAGGGCGGGGGGCGGGGGCGGTTTTTA‑TTCCTGAAAGA‑AGGGACAGTCTGGAAGGACGCTGGGGGGGGCACTCGCCAGAAAAgggg                                                   >  1:1270932/1‑148 (MQ=255)

                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                                <  2:525488/151‑1 (MQ=255)

                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                                 <  1:510653/150‑1 (MQ=255)

                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGAAAGATGCGCT‑GGAAGGGACGGGGGGGGGGGGGGTTGTCA‑TTCC‑GAAAGAGAGGGACAAGCTGGAAGAACGCGGTGGGGGGCAATCGCCAGAGAAggggggt                                                >  2:1649306/1‑146 (MQ=255)

                                                                                                        agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTAGGGGGAAAAAAGGGCT‑GAAAGGGCAGGTGGCGGGGGGGTTTGTCA‑ATGC‑TGAAGAGAGCGGCAACTTGGTAGATCGGCATGTGGTGGCCTCCCAAGAGAAGGGGAtt                                                >  2:1075629/1‑150 (MQ=255)

                                                                                                          tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGGGCT‑GGAAGGGGAGGGGGGGGGGGGGGTTGTTC‑TTTC‑GAAAAAGAGGGAAAGTCTGGGAGAACGGGGTGTGGGGCACCCGCCAGAGAAGGCGAtt                                                >  1:1666196/1‑148 (MQ=255)

                                                                                                                cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTTA‑TTTC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGAtttt                                              >  2:575606/1‑142 (MQ=255)

                                                                                                                cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCT‑GAAAGGGCGGGGGGCGGGGGCGCTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGGGCACTCCCAAGGGAAGGGGGTTATGgcg                                          >  2:1979671/1‑148 (MQ=255)

                                                                                                                 aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                      <  2:464601/151‑1 (MQ=255)

                                                                                                                   ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                     <  1:1477386/150‑1 (MQ=255)

                                                                                                                    cATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTTA‑TTTC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACCCGCAAGAGAAGGGGATTATTGCGCtggtgg                                   >  2:1587286/1‑151 (MQ=255)

                                                                                                                    cATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTCA‑TTTC‑GAAAGAGAGCGACAGTCTGGGAGATCGTGATGGGGCGCACTCGCAAGAGAAGGCGGTTATTGCGCtggggg                                   >  2:923066/1‑151 (MQ=255)

                                                                                                                    cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCT‑GGAAGGGGAGGGGGCGGGGGGGGTTGTCA‑TTTC‑GGAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAGGGGGTTATGGCGCtggtgg                                   >  1:785945/1‑151 (MQ=255)

                                                                                                                       ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                                 <  2:366764/150‑1 (MQ=255)

                                                                                                                       ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                                <  2:1596744/151‑1 (MQ=255)

                                                                                                                       ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                                <  2:60227/151‑1 (MQ=255)

                                                                                                                                 ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                      <  2:224921/151‑1 (MQ=255)

                                                                                                                                   gAAGTGGCGGTGGTTGGGGGGGAAGATGCGCT‑GAAAGGGGCGGGGGGGGGGGGGGTTGTCA‑TTTC‑GCAAGAGAGCGACAGTCTGGGAGAAAGTGATGTGGCGCACTCGCAAGAGAAAGCGATTATGGCGCTGGGGGGGAGCCACATTgg                      >  1:2041877/1‑149 (MQ=255)

                                                                                                                                    aaGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                   <  2:911896/151‑1 (MQ=255)

                                                                                                                                      gTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACt                 <  2:2076238/151‑1 (MQ=255)

                                                                                                                                        ggCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt               >  2:1338479/1‑151 (MQ=255)

                                                                                                                                        ggCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt               >  1:705535/1‑151 (MQ=255)

                                                                                                                                         gCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg              >  2:97128/1‑151 (MQ=255)

                                                                                                                                          cGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg             <  2:1009308/151‑1 (MQ=255)

                                                                                                                                            gtggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  2:1880413/1‑151 (MQ=255)

                                                                                                                                             tggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg          >  1:420/1‑151 (MQ=255)

                                                                                                                                                     gTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  2:2031015/1‑151 (MQ=255)

                                                                                                                                                     gTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  <  1:259641/151‑1 (MQ=255)

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCA‑TTCC‑GAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354138‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`