Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT39.3% 144.9 / 63.9 112S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (16/52);  new base T (43/1);  total (59/53)
Fisher's exact test for biased strand distribution p-value = 2.00e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  NC_000913/4604196‑4604493
                                                                                                                                                    |                                                                                                                                                      
cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                                      >  1:1541389/1‑151 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                      <  2:1960275/149‑1 (MQ=255)
     aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttt                                                                                                                                                 >  1:1672964/1‑149 (MQ=255)
         aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                                                                              >  2:2085634/1‑150 (MQ=255)
         aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                             <  2:435990/151‑1 (MQ=255)
             gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                         <  2:2086448/151‑1 (MQ=255)
              cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATg                                                                                                                                           >  2:1042615/1‑148 (MQ=255)
              cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                        <  2:957965/151‑1 (MQ=255)
              cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                        <  1:1719557/151‑1 (MQ=255)
               gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCATgag                                                                                                                                         >  2:1125015/1‑149 (MQ=255)
                 cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTgtcc                                                                                                                                             >  1:472511/1‑140 (MQ=255)
                 cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                      <  2:278626/150‑1 (MQ=255)
                    gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                                  <  1:1284695/151‑1 (MQ=255)
                     agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                 <  1:1938989/151‑1 (MQ=255)
                      gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGGGAgcg                                                                                                                                >  2:264338/1‑151 (MQ=255)
                       tagttTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgct                                                                                                                                <  1:1133211/150‑2 (MQ=255)
                        agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTTTTTTTTTGCCATGAGGAGTggggg                                                                                                                                >  2:2059664/1‑145 (MQ=255)
                        agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTAATTTTTTCTTTTTTTGCCCTGAGAAGTg                                                                                                                                    >  2:304855/1‑145 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                              <  2:256320/150‑1 (MQ=255)
                           ctCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCaggg                                                                                                                           >  1:1794546/2‑149 (MQ=255)
                             cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                                                                         <  2:1505727/151‑1 (MQ=255)
                             cGGTATTGAGAGGTTATTCAGAAAGTTGGCGGGTAACCCTTATAATGTCCATACCTCTACCATTCATGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCCGTCATTTTTTCTTTTTCTGCCATGAGAAGAGAGCGCagagag                                                                                                                         <  1:1105981/151‑1 (MQ=255)
                              ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGGGAAGGGAGCGCCGAgggg                                                                                                                        >  2:684840/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                       <  1:541098/151‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑tt                                                                                                              <  2:215017/151‑1 (MQ=255)
                                             ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCAGGGAGGGGTTATC‑TTTcc                                                                                                           >  1:506975/1‑148 (MQ=255)
                                               cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCctgact                                                                                                      <  1:1008038/151‑1 (MQ=255)
                                               cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCctgact                                                                                                      <  1:2081418/151‑1 (MQ=255)
                                                 gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAATTTTTTTTTTTTTTCCCGGCGGGGGGAGCGAAGAGCGGGATTAtt‑tt                                                                                                              >  2:502503/1‑141 (MQ=255)
                                                   aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAAc                                                                                                  <  1:424925/151‑1 (MQ=255)
                                                    aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACCGTTTTTTTTTTTTTCTCCTGCGGAGGGAGCGCAGGGAGGGGTTATC‑TTTcccgcatgaa                                                                                                   >  2:537257/1‑141 (MQ=255)
                                                    aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGACGCCATGTCGCGGATCTCGTTTGCGGCGGTCCGTTTTTCTTTTTCTTCCTTGATCAGGGTACCCCGGGAGGGAATATC‑CTGccctacc                                                                                                      >  2:208591/1‑141 (MQ=255)
                                                     gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTTCGAAGTGAGGGGCGAGAGGGATTTTC‑TTTCctgactggaat                                                                                                 >  2:1446887/1‑146 (MQ=255)
                                                        tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGc                                                                                              <  2:673299/150‑1 (MQ=255)
                                                         gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGGGCGCAGAGAGGGGTTATC‑TTGTCTGGATGAACTgggg                                                                                             >  2:1572339/1‑148 (MQ=255)
                                                          cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGAt                                                                                           <  2:391824/151‑1 (MQ=255)
                                                           gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGGAGTGAGCGCAGAGGGGGATTATT‑TTGTCTGACTGAACTGGTGAtt                                                                                          >  2:1451314/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGttt                                                                                    <  1:1991914/151‑1 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATC‑TTGCCTGACTGAAATGGCGATTAAGGTTcc                                                                                  >  2:276915/1‑151 (MQ=255)
                                                                     tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTcc                                                                                  <  2:74230/149‑1 (MQ=255)
                                                                      ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCcgcg                                                                               <  1:1673340/151‑1 (MQ=255)
                                                                       taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGGGAAGTGGGCGCAGAGAGGGGTTATT‑TTGCCTGGCTGAACTGGCGATTAAGttttc                                                                                  >  2:57336/1‑145 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGGGGAGTGGGGGCAGAGAGGGGGTATC‑TTTTCTGTCTTAACTGGGGGTTAAGGTTTCGCGGAccc                                                                          >  1:434133/1‑151 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGGCACGAGACGTGAGGGGGGAGAGGGATTATC‑TTGCCTCACCTTAATGGCGAATTAGGTTGcgcggttcc                                                                          >  2:950471/1‑146 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTCCATGAGAAGTGAGTGCAGGGAGGGATTTTT‑TTTCCTGACTGAACTGGCGGTTAAGTTTCCGCGTAccc                                                                          >  2:334369/1‑151 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGAGGGGTTTTC‑TTTCCTGGCTGAAACGGCGATTAAGTTTTCGCGTAccc                                                                          >  1:1625107/1‑151 (MQ=255)
                                                                            tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGATTATC‑TTTCCTGACTGAACTGGCGATTTAGTTTCCGCGTAccc                                                                          >  2:2090641/1‑150 (MQ=255)
                                                                              cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCCGAGAGGGATTATC‑TTGCCTGACTGGAATGGCGATTAAGTTTCCGCGTACCCgg                                                                        >  2:1635966/1‑150 (MQ=255)
                                                                              cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGGGGGGTTATT‑TTTCCTGACTGAACTGGCGATTTAGTTTTCGCGTAcc                                                                           >  1:138630/1‑147 (MQ=255)
                                                                               aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                                      <  1:918204/151‑1 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                   <  1:44051/151‑1 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                   <  1:834266/151‑1 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                   <  1:274271/151‑1 (MQ=255)
                                                                                   cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTTTC‑TTTCCTTACTGAACTGGCGATTAAATTTTCGCGTACCCCGCGgttt                                                                  >  1:253011/1‑150 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCAGAGAGGGGTTATC‑TTTTCTGACTGAAATGGCGATTAAGTTTCCGCGGACCCCGCGgtt                                                                   >  1:1277176/1‑148 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa                                                                  <  1:1449528/149‑1 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa                                                                  <  2:1672535/149‑1 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                 <  2:102835/150‑1 (MQ=255)
                                                                                         cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                            <  1:612378/151‑1 (MQ=255)
                                                                                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGGGCGCAGAGAGGGGTTATC‑TTGCCTGACTGGACTGGCGGTTAAATTTTCGCGTACCCGGCGGTTAGTTAtt                                                            >  2:1618760/1‑148 (MQ=255)
                                                                                             cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCCTGAGAAGGGAGGGCAGAGAGGGATTTTC‑TTGACTGACTTAAAAGGCGAATAAGTTTCCCCCTCCCCGGCGGTTAGTTTTTGCgg                                                        >  2:1315090/1‑151 (MQ=255)
                                                                                              aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGGGAGGGGAGAGAGGGGGTATT‑TTTCCTGACTTAACTGGCGGTTAAGTTTTCGCGTACCCCGCGTTTTGTTAtt                                                            >  1:1861885/1‑146 (MQ=255)
                                                                                               ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTTCCCGGCGTTTAGTTATTGGGgat                                                      <  1:1449664/151‑1 (MQ=255)
                                                                                               ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                      <  2:803532/151‑1 (MQ=255)
                                                                                                gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                     <  2:997568/151‑1 (MQ=255)
                                                                                                gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                     <  1:720582/151‑1 (MQ=255)
                                                                                                  gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTC‑TTTCCTGACTGAACTGGGGGTTTAGTTTCCGCGTACCCGGGGTTTTGTTTTTTCGggtg                                                     >  1:540470/1‑149 (MQ=255)
                                                                                                  gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATC‑TTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTTTTGCgggt                                                      >  2:979056/1‑146 (MQ=255)
                                                                                                         cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGTAGAGAGCGCAGAGAGGGGGTTTC‑TTGGCAGATTAAACAGGCGGTTAAGGTTGACCCCATCCGGGGGTTTGTTATTGAGGGTGATGgtaat                                             >  2:1407201/1‑148 (MQ=255)
                                                                                                         cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                            <  2:1349937/151‑1 (MQ=255)
                                                                                                         cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGGGGGGGGGTTTT‑TTTTCTGACTGAAATGGCGGGTTAGGTTTCGCGGTCCCCGCGGTTTGTTTTTTGGGGTGGTGAtttt                                             >  1:2075402/1‑150 (MQ=255)
                                                                                                          aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGGGGGGTTATT‑TTGCCTGACTGAAATTGCGATTAAGTTTTCGCGTACCCGGCGGTTTGTTATTTTGGGTGATGATAtt                                             >  1:374038/1‑149 (MQ=255)
                                                                                                            gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCCGAGAGGGATTATC‑TTTCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCCGCGGTTTGTTTTTGCGGGTGATGATATTTaa                                          >  2:1676859/1‑150 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                        <  2:1920344/151‑1 (MQ=255)
                                                                                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTACCGCGAGAAGTGTGAGCAAAGCGGGGTTATCTTTGCCTG‑CTGAATAAGCGATTAAAATTTCGCCGAACCCGCGGGTTGTTAATAATGCTGATGATATTGa                                           >  2:1398275/1‑147 (MQ=255)
                                                                                                               gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTc                                      <  2:2042166/151‑1 (MQ=255)
                                                                                                                ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                     <  1:1116302/151‑1 (MQ=255)
                                                                                                                    gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                  <  1:1166646/150‑1 (MQ=255)
                                                                                                                      cgcgGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGGGGGCGCAGAGAGGGGTTATC‑TTGTCTGACTGAAATGGCGATTAAGTTTTCGCGTACCCGGCGGTTTGTTTTTTCGGGTGATGATATTTAAGCTCGGCTGAt                               >  2:900380/1‑151 (MQ=255)
                                                                                                                       gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                               <  1:655134/150‑1 (MQ=255)
                                                                                                                        cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTTTC‑TTGCCTGACTGAACTTGCGGTTAAGTTTCCGCGTACCCGGCGGTTTGTTATTTCGGGTGATGATATTGAAGCTCCGCTGGtt                              >  2:84185/1‑150 (MQ=255)
                                                                                                                        cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                              <  2:1723761/150‑1 (MQ=255)
                                                                                                                         ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGAGAGCGCCGAGAGGGGTTATC‑TTTCCCGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTTCGGGTGATGATATTTAAACTCGGCTGAtttg                            >  2:248594/1‑149 (MQ=255)
                                                                                                                         ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATT‑TTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTTCGGGTGATGATATTGAAGCCCGGCTGGtt                              >  2:1897173/1‑149 (MQ=255)
                                                                                                                         ggATCCGGTTTGCGGCGGTCTTTTTTTTTTTTTCGGCCATGAGAAGTGAGCGCAGAGGGGAATTATT‑TTCCCTGACGGAACGGGCGATTAATTTCCCGCGTCCCCGGCTTTTAGTTATGGCGGAGAATAATTTTGAAGCTCGGGTGAtt                              <  1:372604/149‑1 (MQ=255)
                                                                                                                               cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                           <  1:996334/146‑1 (MQ=255)
                                                                                                                               cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                       <  2:1899758/150‑1 (MQ=255)
                                                                                                                                   tGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc                   <  1:304848/150‑1 (MQ=255)
                                                                                                                                    gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc                 <  1:500864/151‑1 (MQ=255)
                                                                                                                                       gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt              >  1:1011721/1‑151 (MQ=255)
                                                                                                                                        cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc             >  2:1063786/1‑151 (MQ=255)
                                                                                                                                           tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          >  2:1428240/1‑151 (MQ=255)
                                                                                                                                            cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  2:2053509/1‑151 (MQ=255)
                                                                                                                                            cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         <  2:1903925/151‑1 (MQ=255)
                                                                                                                                            cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  1:515520/1‑151 (MQ=255)
                                                                                                                                            cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         <  1:1966797/151‑1 (MQ=255)
                                                                                                                                            cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  1:1379545/1‑151 (MQ=255)
                                                                                                                                                  ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   >  1:905699/1‑151 (MQ=255)
                                                                                                                                                   tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg  >  2:1260684/1‑151 (MQ=255)
                                                                                                                                                   tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg  >  1:1970874/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  NC_000913/4604196‑4604493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑