Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3500CT39.3% 157.9 / 52.8 112S64F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (15/53);  new base T (44/0);  total (59/53)
Fisher's exact test for biased strand distribution p-value = 1.37e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGT  >  NC_000913/4604205‑4604495
                                                                                                                                                  |                                                                                                                                                  
aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                                                                                >  2:2085634/1‑150 (MQ=255)
aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                               <  2:435990/151‑1 (MQ=255)
    gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                           <  2:2086448/151‑1 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATg                                                                                                                                             >  2:1042615/1‑148 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                          <  2:957965/151‑1 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                          <  1:1719557/151‑1 (MQ=255)
        cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                        <  2:278626/150‑1 (MQ=255)
           gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                                    <  1:1284695/151‑1 (MQ=255)
            agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                   <  1:1938989/151‑1 (MQ=255)
             gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGGGAgcg                                                                                                                                  >  2:264338/1‑151 (MQ=255)
              tagttTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgct                                                                                                                                  <  1:1133211/150‑2 (MQ=255)
               agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTTTTTTTTTGCCATGAGGAGTggggg                                                                                                                                  >  2:2059664/1‑145 (MQ=255)
               agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTAATTTTTTCTTTTTTTGCCCTGAGAAGTg                                                                                                                                      >  2:304855/1‑145 (MQ=255)
                  ctCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCaggg                                                                                                                             >  1:1794546/2‑149 (MQ=255)
                    cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                                                                           <  2:1505727/151‑1 (MQ=255)
                    cGGTATTGAGAGGTTATTCAGAAAGTTGGCGGGTAACCCTTATAATGTCCATACCTCTACCATTCATGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCCGTCATTTTTTCTTTTTCTGCCATGAGAAGAGAGCGCagagag                                                                                                                           <  1:1105981/151‑1 (MQ=255)
                     ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGGGAAGGGAGCGCCGAgggg                                                                                                                          >  2:684840/1‑151 (MQ=255)
                            agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTTGGAAGGGGGCGGCGAGAggggatat                                                                                                                    >  2:1900209/1‑145 (MQ=255)
                              agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAAAGAGAGCGGAGAggg                                                                                                                         >  2:2065961/1‑143 (MQ=255)
                              agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                                <  2:215017/151‑1 (MQ=255)
                                    ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCAGGGAGGGGTTATCTTTcc                                                                                                             >  1:506975/1‑148 (MQ=255)
                                      cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                        <  1:1008038/151‑1 (MQ=255)
                                      cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                        <  1:2081418/151‑1 (MQ=255)
                                       aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAATTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGtttttttttctggct                                                                                                         >  1:1508865/1‑137 (MQ=255)
                                        gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTCTTCCCTGAGGAGAGAGAGCCGAGAGGGATTTTCTTTCctgtctg                                                                                                       >  2:1650694/1‑150 (MQ=255)
                                        gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAATTTTTTTTTTTTTTCCCGGCGGGGGGAGCGAAGAGCGGGATTAtttt                                                                                                                >  2:502503/1‑141 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                                    <  1:424925/151‑1 (MQ=255)
                                           aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACCGTTTTTTTTTTTTTCTCCTGCGGAGGGAGCGCAGGGAGGGGTTATCTTTcccgcatgaa                                                                                                     >  2:537257/1‑141 (MQ=255)
                                            gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTTCGAAGTGAGGGGCGAGAGGGATTTTCTTTCctgactggaat                                                                                                   >  2:1446887/1‑146 (MQ=255)
                                               tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                                <  2:673299/150‑1 (MQ=255)
                                                gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGGGCGCAGAGAGGGGTTATCTTGTCTGGATGAACTgggg                                                                                               >  2:1572339/1‑148 (MQ=255)
                                                  gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGGAGTGAGCGCAGAGGGGGATTATTTTGTCTGACTGAACTGGTGAtt                                                                                            >  2:1451314/1‑151 (MQ=255)
                                                        cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                                      <  1:1991914/151‑1 (MQ=255)
                                                         ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                                      >  2:1209842/1‑150 (MQ=255)
                                                          cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAAATGGCGATTAAGGTTcc                                                                                    >  2:276915/1‑151 (MQ=255)
                                                            tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTcc                                                                                    <  2:74230/149‑1 (MQ=255)
                                                             ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgcg                                                                                 <  1:1673340/151‑1 (MQ=255)
                                                              taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGGGAAGTGGGCGCAGAGAGGGGTTATTTTGCCTGGCTGAACTGGCGATTAAGttttc                                                                                    >  2:57336/1‑145 (MQ=255)
                                                                  cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGGGGAGTGGGGGCAGAGAGGGGGTATCTTTTCTGTCTTAACTGGGGGTTAAGGTTTCGCGGAccc                                                                            >  1:434133/1‑151 (MQ=255)
                                                                  cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGGCACGAGACGTGAGGGGGGAGAGGGATTATCTTGCCTCACCTTAATGGCGAATTAGGTTGcgcggttcc                                                                            >  2:950471/1‑146 (MQ=255)
                                                                  cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTCCATGAGAAGTGAGTGCAGGGAGGGATTTTTTTTCCTGACTGAACTGGCGGTTAAGTTTCCGCGTAccc                                                                            >  2:334369/1‑151 (MQ=255)
                                                                  cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGAGGGGTTTTCTTTCCTGGCTGAAACGGCGATTAAGTTTTCGCGTAccc                                                                            >  1:1625107/1‑151 (MQ=255)
                                                                   tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTTAGTTTCCGCGTAccc                                                                            >  2:2090641/1‑150 (MQ=255)
                                                                     cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCCGAGAGGGATTATCTTGCCTGACTGGAATGGCGATTAAGTTTCCGCGTACCCgg                                                                          >  2:1635966/1‑150 (MQ=255)
                                                                     cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGGGGGGTTATTTTTCCTGACTGAACTGGCGATTTAGTTTTCGCGTAcc                                                                             >  1:138630/1‑147 (MQ=255)
                                                                     cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGc                                                                         <  2:1438279/151‑1 (MQ=255)
                                                                         ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                     <  1:834266/151‑1 (MQ=255)
                                                                         ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                     <  1:44051/151‑1 (MQ=255)
                                                                         ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                     <  1:274271/151‑1 (MQ=255)
                                                                          cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTCCTTACTGAACTGGCGATTAAATTTTCGCGTACCCCGCGgttt                                                                    >  1:253011/1‑150 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCAGAGAGGGGTTATCTTTTCTGACTGAAATGGCGATTAAGTTTCCGCGGACCCCGCGgtt                                                                     >  1:1277176/1‑148 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa                                                                    <  1:553918/149‑1 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa                                                                    <  1:1449528/149‑1 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa                                                                    <  2:1672535/149‑1 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                   <  2:102835/150‑1 (MQ=255)
                                                                                cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCATTTTTTTTTTTTCGCTCTGCGGAGTGTGGGCGGGGAGGGTTTAATTTTCCTTACTGATATGGCGATTTATTTTTCGCGGTCCAGTCGCTTTGTTAtt                                                              >  2:307317/1‑151 (MQ=255)
                                                                                cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                              <  1:612378/151‑1 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGGGCGCAGAGAGGGGTTATCTTGCCTGACTGGACTGGCGGTTAAATTTTCGCGTACCCGGCGGTTAGTTAtt                                                              >  2:1618760/1‑148 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCCTGAGAAGGGAGGGCAGAGAGGGATTTTCTTGACTGACTTAAAAGGCGAATAAGTTTCCCCCTCCCCGGCGGTTAGTTTTTGCgg                                                          >  2:1315090/1‑151 (MQ=255)
                                                                                     aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGGGAGGGGAGAGAGGGGGTATTTTTCCTGACTTAACTGGCGGTTAAGTTTTCGCGTACCCCGCGTTTTGTTAtt                                                              >  1:1861885/1‑146 (MQ=255)
                                                                                      ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                        <  2:803532/151‑1 (MQ=255)
                                                                                       gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                       <  2:997568/151‑1 (MQ=255)
                                                                                       gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                       <  1:720582/151‑1 (MQ=255)
                                                                                         gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTCTTTCCTGACTGAACTGGGGGTTTAGTTTCCGCGTACCCGGGGTTTTGTTTTTTCGggtg                                                       >  1:540470/1‑149 (MQ=255)
                                                                                         gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTTTTGCgggt                                                        >  2:979056/1‑146 (MQ=255)
                                                                                          tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTTCCTGCGAAGGGAGGGCGGGGAGGGGGTTTCTTGCCTGGCTGAACTGGCGGTTAAGTTTTCGCGGACCCGGCGGTTTGTTATTTCggggggt                                                     >  1:500003/1‑145 (MQ=255)
                                                                                                cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                              <  2:1349937/151‑1 (MQ=255)
                                                                                                cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGGGGGGGGGTTTTTTTTCTGACTGAAATGGCGGGTTAGGTTTCGCGGTCCCCGCGGTTTGTTTTTTGGGGTGGTGAtttt                                               >  1:2075402/1‑150 (MQ=255)
                                                                                                 aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCTTGAGAAGTGAGGGCAGAGAGGGGTTATATTTTCCGACCGAACTGGCGGTTAAGATTCTCCGGACCCGGCGCTTTGTTTTTTCGGGTGATGAtttt                                               >  2:230891/1‑149 (MQ=255)
                                                                                                 aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGGGGGGTTATTTTGCCTGACTGAAATTGCGATTAAGTTTTCGCGTACCCGGCGGTTTGTTATTTTGGGTGATGATAtt                                               >  1:374038/1‑149 (MQ=255)
                                                                                                     tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTACCGCGAGAAGTGTGAGCAAAGCGGGGTTATCTTTGCCTGCTGAATAAGCGATTAAAATTTCGCCGAACCCGCGGGTTGTTAATAATGCTGATGATATTGa                                             >  2:1398275/1‑147 (MQ=255)
                                                                                                      gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTc                                        <  2:2042166/151‑1 (MQ=255)
                                                                                                       ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                       <  1:1116302/151‑1 (MQ=255)
                                                                                                           gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                    <  1:1166646/150‑1 (MQ=255)
                                                                                                             cgcgGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGGGGGCGCAGAGAGGGGTTATCTTGTCTGACTGAAATGGCGATTAAGTTTTCGCGTACCCGGCGGTTTGTTTTTTCGGGTGATGATATTTAAGCTCGGCTGAt                                 >  2:900380/1‑151 (MQ=255)
                                                                                                              gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                                 <  1:655134/150‑1 (MQ=255)
                                                                                                               cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTTTCTTGCCTGACTGAACTTGCGGTTAAGTTTCCGCGTACCCGGCGGTTTGTTATTTCGGGTGATGATATTGAAGCTCCGCTGGtt                                >  2:84185/1‑150 (MQ=255)
                                                                                                               cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                                <  2:1723761/150‑1 (MQ=255)
                                                                                                                ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGAGAGCGCCGAGAGGGGTTATCTTTCCCGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTTCGGGTGATGATATTTAAACTCGGCTGAtttg                              >  2:248594/1‑149 (MQ=255)
                                                                                                                ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTTCGGGTGATGATATTGAAGCCCGGCTGGtt                                >  2:1897173/1‑149 (MQ=255)
                                                                                                                ggATCCGGTTTGCGGCGGTCTTTTTTTTTTTTTCGGCCATGAGAAGTGAGCGCAGAGGGGAATTATTTTCCCTGACGGAACGGGCGATTAATTTCCCGCGTCCCCGGCTTTTAGTTATGGCGGAGAATAATTTTGAAGCTCGGGTGAtt                                <  1:372604/149‑1 (MQ=255)
                                                                                                                      cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                             <  1:996334/146‑1 (MQ=255)
                                                                                                                      cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                         <  2:1899758/150‑1 (MQ=255)
                                                                                                                          tGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc                     <  1:304848/150‑1 (MQ=255)
                                                                                                                           gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc                   <  1:500864/151‑1 (MQ=255)
                                                                                                                              gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt                >  1:1011721/1‑151 (MQ=255)
                                                                                                                               cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc               >  2:1063786/1‑151 (MQ=255)
                                                                                                                                  tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc            >  1:928593/1‑151 (MQ=255)
                                                                                                                                  tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc            >  2:1428240/1‑151 (MQ=255)
                                                                                                                                    cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc            <  2:537451/150‑1 (MQ=255)
                                                                                                                                    cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg           >  1:515520/1‑151 (MQ=255)
                                                                                                                                    cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg           <  1:1966797/151‑1 (MQ=255)
                                                                                                                                    cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg           >  1:1379545/1‑151 (MQ=255)
                                                                                                                                    cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg           >  2:2053509/1‑151 (MQ=255)
                                                                                                                                    cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg           <  2:1903925/151‑1 (MQ=255)
                                                                                                                                          ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc     >  1:905699/1‑151 (MQ=255)
                                                                                                                                           tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg    >  1:1970874/1‑151 (MQ=255)
                                                                                                                                           tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg    >  2:1260684/1‑151 (MQ=255)
                                                                                                                                             tttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGgt  >  2:1604859/1‑151 (MQ=255)
                                                                                                                                              ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACgg   <  1:1591151/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGT  >  NC_000913/4604205‑4604495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: