Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,1590GA40.4% 128.7 / 75.6 110intergenic (+139/‑75)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (58/7);  new base A (0/44);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 1.76e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

ATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAA  >  NC_000913/5010‑5308
                                                                                                                                                           |                                                                                                                                                     
atcatcAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGgaga                                                                                                                                                      <  1:1766172/151‑1 (MQ=255)
 tcatcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGgaga                                                                                                                                                      >  1:1441796/1‑150 (MQ=255)
  catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAAt                                                                                                                                                    <  1:727296/151‑1 (MQ=255)
    tcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATa                                                                                                                                                   >  2:20426/1‑150 (MQ=255)
     caGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGGGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATaa                                                                                                                                                  <  2:985032/150‑1 (MQ=255)
       gTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATaacaa                                                                                                                                               <  2:1106928/151‑1 (MQ=255)
       gTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATaaca                                                                                                                                                >  2:1220756/1‑150 (MQ=255)
         aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAAcc                                                                                                                                             >  1:1142744/1‑151 (MQ=255)
               tATTCATTATCTAAATCAGGCGGGGTTTGCTTTTATGCAGCCCGGTTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGAAAAAA‑GGAGAAA‑TT‑CTCAAAAAATGCGGTAACTT‑AGAGATTAGGATG‑GCGGAAAATAACAAccgccg                                                                                                                                         <  1:1403424/149‑1 (MQ=255)
                 ttCATTATCTAAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGTTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGAAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTtc                                                                                                                                      <  2:410421/150‑1 (MQ=255)
                    aTTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCAt                                                                                                                                  >  2:1994775/1‑151 (MQ=255)
                     ttATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATc                                                                                                                                 >  2:1843971/1‑151 (MQ=255)
                       aTCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCg                                                                                                                                >  1:1967665/1‑150 (MQ=255)
                       aTCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCg                                                                                                                                >  2:1672240/1‑150 (MQ=255)
                         ctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAg                                                                                                                              >  1:1175081/1‑150 (MQ=255)
                           cAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTa                                                                                                                            >  2:449504/1‑150 (MQ=255)
                             aTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAAtc                                                                                                                         >  1:417273/1‑151 (MQ=255)
                              tcgggCGGGGTTTGTTTTTATGCAGCCCGGTTTTTTTAAAAAAAAATTATGGAAAAAAATG‑ACGGGAAAAAA‑GAAAAAA‑TT‑CTAAAAAAATGCGTTAACTT‑AGAGATTAGGATG‑GCGGAAAAAAAAAACCGCCGTTCTCATCGAGTaa                                                                                                                           <  2:1733548/145‑1 (MQ=255)
                                aGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTcc                                                                                                                      >  1:70920/1‑151 (MQ=255)
                                 ggcgggttttttttttAGGCACCCGGGTTTTTTTAAAAAAAAATTAGGAAAAAAAAGG‑AAAGGAAAAAA‑GAAAAAA‑TT‑CAAAAAAAAGGCGATAACTT‑AGATATGAGGATG‑GAGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCg                                                                                                                     <  1:409221/139‑1 (MQ=255)
                                 ggCGGGGTTTGTTTTAATGCGGCCGGGTTTTTTTAGAAAAAAATTAGGAAAAAAAATG‑AAAGGAAAAAA‑GGAAAAA‑TT‑CTAAAAAATGGCGGTAATTT‑AGAGATTAGAATT‑GGGGAAAATAACAACCGCCGTTCTCATCGAGTAATCTcc                                                                                                                      <  2:1942745/150‑1 (MQ=255)
                                   ccGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCgg                                                                                                                    >  2:1767301/1‑150 (MQ=255)
                                    gcgttttGATTTAATCCGGCCCTGTTTTTTTAAAAAAAAATAAGGAAAAAAAAGG‑CAAGAGAAAAA‑GGAAAAT‑TT‑CTAAAAAATGGCGGTAGTTA‑AGAATTAAGGATT‑GGGGAGAATACCAGCCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                                  <  1:308080/147‑1 (MQ=255)
                                     gggTTTTTTTTTATGAACCCGGGTTTTTTTATAAAAAAATTATGGAAAAAAATG‑ACGGGAAAAAA‑GGAGAAA‑TT‑CTAAAAAAAGGCGTTAATTA‑AGAGATTAGGATG‑GCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                                  <  2:629683/150‑1 (MQ=255)
                                     gggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                                  >  2:30514/1‑150 (MQ=255)
                                      ggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGatat                                                                                                                >  2:1380491/1‑151 (MQ=255)
                                        tttGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc                                                                                                               >  2:693842/1‑150 (MQ=255)
                                        tttGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCg                                                                                                              >  2:1455645/1‑151 (MQ=255)
                                             ttttATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAccc                                                                                                          >  2:100611/1‑150 (MQ=255)
                                                tATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATa                                                                                                       >  2:1195947/1‑150 (MQ=255)
                                                  tGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACg                                                                                                    >  1:1525375/1‑151 (MQ=255)
                                                    cAGCCGGGTTTTTTTATAAAAAAATTAGGGAAAAAAATG‑AAAGGAAAAAA‑GGAAAAA‑TT‑CTAAATAAAGGCGTTAATTT‑AGAGATAGGGATG‑GGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACggg                                                                                                  <  2:1036064/151‑1 (MQ=255)
                                                       cccGGTTTTTTTTAAAAAAAATTAAGGAAAAAAAAG‑AAAAGGAAAAAGGGAGAAA‑TT‑CTAAATAAA‑GGGGTAAATT‑GAAAATTTAGATTGGGGGAAAAAAAAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCa                                                                                                <  2:1608046/151‑1 (MQ=255)
                                                        cccggttttttAAAAAAAAAATAAGGAAAAAAAAGAAGAGAAAAAAA‑GGAAAAT‑TC‑CTAAAAAATTGCGGTAACTA‑AAAAATTAGAATG‑GCGAAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc                                                                                                     <  1:339219/140‑1 (MQ=255)
                                                        ccGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAAt                                                                                              >  1:1877424/1‑151 (MQ=255)
                                                        ccGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAAt                                                                                              >  2:2081119/1‑151 (MQ=255)
                                                         ggttttttttAAAAAAAAATTAGGGAAAAAAAGG‑AAAGGAAAAAA‑GGAAAAT‑TC‑CTAAATAAATGCGTTAATTA‑AGATATAAGTTTT‑GGGGAAAATAACAGCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATg                                                                                             <  2:234460/147‑1 (MQ=255)
                                                           gCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                                           >  2:1236330/1‑151 (MQ=255)
                                                               ttttAAAAAAAATTTAGGGAAAAAAATG‑ACAGGAAAAAA‑GAAAAAT‑TT‑TTAAAAAAGTGAGAAATTTT‑AGAGATAGGGTTT‑GGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa                                                                                        <  2:194427/150‑1 (MQ=255)
                                                                 ttaaaaaaaaaTAATGAAAAAAAAGG‑AAAGAAAAAAA‑GGAAAAA‑TT‑CTAAAAAAATGCGTTAACTT‑AAAGATAAGAATG‑GCGAAAAAAAACATCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAg                                                                                      <  1:53116/142‑1 (MQ=255)
                                                                 ttATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                     >  1:8533/1‑151 (MQ=255)
                                                                  tATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                     >  2:777931/1‑150 (MQ=255)
                                                                     gaagaaATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                  >  1:330431/1‑150 (MQ=255)
                                                                      aaaaattggtGGAAAAAAAAG‑GAAGGAAAAAA‑GGAAAAA‑TT‑AAAAATAAGTGCGGAAACTA‑AGAGTTTTGATTG‑GAGGAAAATACAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTa                                                                                 <  2:960551/141‑1 (MQ=255)
                                                                      aaaaaaTTAAGAAAAAAAATG‑ACAAAAAAAAA‑GAAAAAA‑TC‑CTAAAAAAATGCGTTAACTA‑GGAAATTAGGTGT‑GCGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                     <  1:1820429/143‑1 (MQ=255)
                                                                       agaaATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAc                                                                               >  1:1542071/1‑151 (MQ=255)
                                                                        gaaATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc                                                                              >  1:1687116/1‑151 (MQ=255)
                                                                            ttATGGAG‑AAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                          >  1:1142450/1‑150 (MQ=255)
                                                                               tGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATACATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGCTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGa                                                                         >  1:1826573/1‑149 (MQ=255)
                                                                               tGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaa                                                                        >  1:1800487/1‑150 (MQ=255)
                                                                               agggaaaaaaaG‑GCAGGAAAAAA‑GGAAAAATTT‑CCAAAAAAATGTGTTAACTA‑GAATATGGGTATG‑GGGGAAAAAAATAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCt                                                                             <  2:658273/141‑1 (MQ=255)
                                                                                 gaaaaaaaaG‑AAAAGGAAAAA‑AGAAAAA‑TTCCTAAATAAAGGGGGTAACTA‑AGAAATAAGGATG‑GCGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                          <  1:1440261/144‑1 (MQ=255)
                                                                                  agaAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaaa                                                                      >  2:586080/1‑149 (MQ=255)
                                                                                  aaaaaaaTG‑AAAGGAAAAAA‑GAAAAAA‑TT‑CT‑AAAAAATGGGGTTACTTAAGATATAAGGTTG‑GGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                          <  1:1817703/143‑1 (MQ=255)
                                                                                  aaaaaaaGG‑ACAGGAAAAAA‑GGAAAAT‑TC‑CTCAAGACATTCGTTGATGG‑GCAGAAGAGGATG‑GCGAATAAACACGGCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAGCCtgt                                                                           <  1:1675765/142‑1 (MQ=255)
                                                                                  aaaaaaaGG‑ACAGGAAAAAA‑GGAAAAA‑TT‑CTAAAAAAATGCGTTAATTA‑AGAGTTTAGGATG‑GGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaaaa                                                                     <  2:1514475/148‑1 (MQ=255)
                                                                                  aaaaaaaGG‑AAAGGAAAAAG‑GAAAAAA‑TT‑CTAAATAAATGGGGTAATTA‑AGAGATAAGGATC‑GCGAAAAAAACCACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGa                                                                         <  1:1831193/144‑1 (MQ=255)
                                                                                   aaaaaaTG‑ATGGGAAAAAA‑GGAAAAT‑TT‑CCAAATAGATGCGGTACCTA‑AGAGATGGGGATG‑GGAAAAAATAAGAGCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaa                                                                        <  2:398326/145‑1 (MQ=255)
                                                                                     aaaaTG‑ACAGAAAAAAA‑GGAAAAA‑TT‑CAAAAAAAATGCGGTAACTA‑AGAGTTTGGGATG‑GCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATg                                                                 <  1:1773281/151‑1 (MQ=255)
                                                                                      aaaTG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGc                                                                >  2:2018407/1‑151 (MQ=255)
                                                                                       aaTG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGc                                                                >  1:1680764/1‑150 (MQ=255)
                                                                                        aaagagaGGAAAAAA‑GAAAAAT‑TT‑CAAAATAAAGGGTGTAATTA‑AGAGATTAGGTTG‑GGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCa                                                               <  1:1853270/145‑1 (MQ=255)
                                                                                         aacaaaaggAAAAA‑GGAGAAATTT‑CCAAATAAATGCGATACTTG‑AGATATGAGGTAT‑GGGGAAAAAACCACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCa                                                               <  1:272700/144‑1 (MQ=255)
                                                                                              aGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTAt                                                         >  1:1274961/1‑151 (MQ=255)
                                                                                               ggaaaaaa‑GGAAAAA‑TT‑CAAAAAAAATGGGTTAATTT‑AGAGATTAGGATG‑GGGAAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTa                                                          <  2:1582703/146‑1 (MQ=255)
                                                                                               ggaaaaaa‑GAAAAAT‑TT‑CTAAAAAAAGGCGGAAATTT‑AGAGATTGGGTTT‑GCGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATc                                                        <  2:559676/148‑1 (MQ=255)
                                                                                               gaaaaaaa‑GAAAAAA‑TT‑CCAAAAAAAGGCGGTAACTA‑AGAGATAGGGATG‑GGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTa                                                          <  1:1762472/146‑1 (MQ=255)
                                                                                                  aaaaa‑GGAAAAA‑TC‑CTAAAAAAGTGCGGTATTTT‑AGAGATAGGAATG‑GGGGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTAt                                                         <  2:1066380/147‑1 (MQ=255)
                                                                                                  aaaaa‑GAAAAAA‑TT‑CACAAAAAATGCGGTAATTT‑AGAGATTAGGATG‑GGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTa                                                     <  1:489200/151‑1 (MQ=255)
                                                                                                    aaa‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                                   >  1:1315888/1‑151 (MQ=255)
                                                                                                    aaa‑GGAAAAA‑TT‑CTAAAAAAAGGCGTTAATTT‑AGAGTTAAGGATG‑GGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                                   <  2:310361/151‑1 (MQ=255)
                                                                                                        ggAAAAA‑TT‑CTAAAAAAATGCGTTAATTA‑AGAGATTAGGATT‑GGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGc                                                <  2:841835/151‑1 (MQ=255)
                                                                                                         gagaAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGc                                                >  1:939865/1‑150 (MQ=255)
                                                                                                          agaAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCAc                                              >  1:1437923/1‑151 (MQ=255)
                                                                                                              a‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTc                                          >  1:1912217/1‑151 (MQ=255)
                                                                                                                tt‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTcc                                         >  1:1162480/1‑151 (MQ=255)
                                                                                                                tt‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTcc                                         >  2:1254293/1‑151 (MQ=255)
                                                                                                                   ctcAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                                       >  2:2068/1‑151 (MQ=255)
                                                                                                                    tcAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTg                                      >  2:1059982/1‑151 (MQ=255)
                                                                                                                      aaaaattGCGGTAACTA‑AGAGATCAGGATT‑CGGGAGAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTccc                                        <  2:829153/141‑1 (MQ=255)
                                                                                                                      aaaaaaGGCGGTAATTT‑AGAGATTAGGATT‑GCGGAAAATAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                                       <  2:223805/145‑1 (MQ=255)
                                                                                                                      aaTAAAGGCGTTAACTT‑AGAGATTAGGATG‑GCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTc                                          <  2:117112/145‑1 (MQ=255)
                                                                                                                       aaaatgggggTAACTA‑AGAATTTAGGATC‑GGGAAGAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGtt                                   <  2:1592682/143‑1 (MQ=255)
                                                                                                                        aaaaGGCGGTAACTT‑AGAGATTAGGATG‑GGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTc                                  <  1:1223112/150‑1 (MQ=255)
                                                                                                                              cGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg                            >  1:1514195/1‑151 (MQ=255)
                                                                                                                                gTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCt                          >  2:333575/1‑151 (MQ=255)
                                                                                                                                  aaCTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTcc                        >  1:1673705/1‑151 (MQ=255)
                                                                                                                                   aattaaaaaaTTAGGATG‑GCGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCt                          <  1:1635760/140‑1 (MQ=255)
                                                                                                                                     tt‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCa                      >  1:1976760/1‑150 (MQ=255)
                                                                                                                                     tt‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt                     >  1:589901/1‑151 (MQ=255)
                                                                                                                                             ttAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagc               >  2:1244019/1‑150 (MQ=255)
                                                                                                                                             ttAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagc               >  2:1853351/1‑150 (MQ=255)
                                                                                                                                               aGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac             >  1:1721154/1‑150 (MQ=255)
                                                                                                                                                ggATT‑GGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagca              <  2:505351/148‑1 (MQ=255)
                                                                                                                                                ggATG‑GCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac             <  2:593896/149‑1 (MQ=255)
                                                                                                                                                 ggttgggggAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTccc                       <  2:1407433/132‑1 (MQ=255)
                                                                                                                                                 gATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAgg          >  1:1100461/1‑151 (MQ=255)
                                                                                                                                                    t‑GCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCt        <  1:61361/150‑1 (MQ=255)
                                                                                                                                                        ggAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg    >  1:1958977/1‑151 (MQ=255)
                                                                                                                                                        ggAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCg     <  2:1820286/150‑1 (MQ=255)
                                                                                                                                                           |                                                                                                                                                     
ATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATG‑ACAGGGAAAAA‑GGAGAAA‑TT‑CTCAATAAATGCGGTAACTT‑AGAGATTAGGATT‑GCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAA  >  NC_000913/5010‑5308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑