Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913786,8550AC39.7% 232.1 / 27.2 126G247G (GGT→GGG) gpmAphosphoglyceromutase 1
Reads supporting (aligned to +/- strand):  ref base A (62/14);  new base C (0/50);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 1.21e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTC  >  NC_000913/786706‑787002
                                                                                                                                                     |                                                                                                                                                   
cGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTAc                                                                                                                                                    >  2:529568/1‑151 (MQ=255)
 gTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTAcc                                                                                                                                                   >  2:1405587/1‑151 (MQ=255)
 gTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTAcc                                                                                                                                                   >  1:1333812/1‑151 (MQ=255)
   cAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCt                                                                                                                                                 <  1:1906503/151‑1 (MQ=255)
     gagTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTgg                                                                                                                                               <  1:1580253/151‑1 (MQ=255)
      agTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGt                                                                                                                                              >  1:262578/1‑151 (MQ=255)
       gTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGtt                                                                                                                                             <  2:406001/151‑1 (MQ=255)
       gTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGtt                                                                                                                                             >  2:1062999/1‑151 (MQ=255)
            gTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCa                                                                                                                                         >  1:508299/1‑150 (MQ=255)
            gTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCa                                                                                                                                         >  1:1512518/1‑150 (MQ=255)
              aTCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAAc                                                                                                                                       <  1:1059447/150‑1 (MQ=255)
                ccGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAAccg                                                                                                                                     <  2:1458897/150‑1 (MQ=255)
                 cGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAAccgcc                                                                                                                                   >  1:1443008/1‑151 (MQ=255)
                  gATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAAccgccg                                                                                                                                  >  2:464179/1‑151 (MQ=255)
                    tGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgct                                                                                                                                >  1:1221113/1‑151 (MQ=255)
                    tGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgct                                                                                                                                >  1:1754200/1‑151 (MQ=255)
                           ccacAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAAAAAAAAAGCCGACTCACTTGCAGTCGGTTTTCTCATTTAAAACGAAGGACGTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTc                                                                                                                          <  2:1313665/150‑1 (MQ=255)
                            cacaGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGc                                                                                                                        >  1:801869/1‑151 (MQ=255)
                              caGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTg                                                                                                                      >  1:2024435/1‑151 (MQ=255)
                              caGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTg                                                                                                                      >  1:702238/1‑151 (MQ=255)
                               aGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGc                                                                                                                     >  1:1154729/1‑151 (MQ=255)
                                 gCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCCTTCGCTGCg                                                                                                                    >  2:1784770/1‑150 (MQ=255)
                                  ccTGATAAGTCGCGCAGCGTCGCATCGGGCAATGTGCTCCATTGTTAGCAACAAAAAACCCGACTCTCTTGCAGTCGGTTTTCTCATTTAAAACGAATGACGTTTACTTCGCTTTACCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGAt                                                                                                                  <  1:608918/151‑1 (MQ=255)
                                      aTAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCg                                                                                                              >  2:1914299/1‑151 (MQ=255)
                                      aTAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAAAAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTAAAACAAATGACTTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAtct                                                                                                                <  2:944953/149‑1 (MQ=255)
                                      aTAAGCCGCGCGGCGTCGCATCGGGCAATGTGCTCCATTGTTAGCAAAAAAAAACCCGCCTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATGACGTTTACTTCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGAtctc                                                                                                               <  2:1880755/150‑1 (MQ=255)
                                        aaGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTc                                                                                                            >  1:2088918/1‑151 (MQ=255)
                                        aaGCCGCGCAGCGTCGCATCAGGCATTGTCCTCCATTGTTAGCAAAAAAAAAGCCGACTCACTTGCGGTCGGCTTTCTCATTTTAAACGAATGGCGTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGt                                                                                                             <  1:836059/150‑1 (MQ=255)
                                         aGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTc                                                                                                            >  2:240276/1‑150 (MQ=255)
                                           tCGCGCAGCGTCGCATCAGGCAATGGCCCCCATTGTTAGCAAAAAAAAAGCCGACTCGCTTGCAGTCGGCTTTCTCATTTTAAACGAAGGACTTTTACTTCGTTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAg                                                                                                          <  1:602170/150‑1 (MQ=255)
                                               gcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt                                                                                                      >  1:477348/1‑150 (MQ=255)
                                                cAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAc                                                                                                    >  2:270076/1‑151 (MQ=255)
                                                      cGCATCAGGCAATGTGCTCCGTTGTAAACAAAAAAAAAGCCGCCTCACTTGCAGCCGGCTTTCTCATTTTAAACGAAGGACTTTTACTTCGTTTTCCCCGGTTTTGCAGCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAg                                                                                                <  1:991091/149‑1 (MQ=255)
                                                      cGCAGCGGGCAATGTGCCCCATTGTTAGCAAAAAAAAAGCCGACTCACTTGCAGTCGTTTTTCTCATTTAAAACGAATGACTTTTCCTTCGCTTTCCCCGGGTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGat                                                                                              <  1:1003109/151‑1 (MQ=255)
                                                        cATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGata                                                                                             >  1:1480235/1‑150 (MQ=255)
                                                        cATCAGGCAATGTGCTCCATTGTTACCAACAAAAAAGCAGACTCGCTTGCAGTCGGTTTTCTCTTTTAAAACGAATGACTTTTACTTCGCTTTCCCTTGGTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAc                                                                                                    <  2:624916/143‑1 (MQ=255)
                                                         aTCAGCCATTGTGCCCCATTGTAAACAAAAAAAACCCCCACTCACTTGCTGTCGGTTTTCTCATTTTAAGCGAATGACGTTTCCTTCCCTTTCCCCTTGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGataa                                                                                            <  1:1652592/150‑1 (MQ=255)
                                                           cAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGataat                                                                                           >  2:191825/1‑149 (MQ=255)
                                                            aGGAAATGTGCTCTTTTTTTAGAAAAAAAAAGGCCGACTCACTTGCGGTCGGTTTTCCAATTTAAAAGAAATGACTTTTACTGCTCTTTCCCCTGGTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGc                                                                                        <  2:1739139/151‑1 (MQ=255)
                                                             ggCAATGTGCTCCATTGTTAGCAAAAAAAAAGCCGACTCCCTTGCAGTCGGTTTTCTCATTTTAAACGAATGAAGTTTACTTCGCTTTCCCTTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGataata                                                                                          <  2:1049494/148‑1 (MQ=255)
                                                             gaaaaTGTCCTCCTTTTTTACCAAAAAAAAAGCCGCCTCTCTTGCAGTCGTCTTTCTCATTTAAAACTAATGACGTTTCCTTCTTTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGataat                                                                                           <  1:1639810/144‑1 (MQ=255)
                                                                   gtgCTCCTTTTTTAGAAAAAAAAAAGCCGACTCACTTGCAGTCGTTTTTCTCATTTAAAACGAATGCCGTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGttt                                                                                    <  1:731249/148‑1 (MQ=255)
                                                                    tgCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGc                                                                                >  1:1935520/1‑151 (MQ=255)
                                                                      ccccATTTTTAGAAACAAAAAAGCCCGCTCCCTGGCGGTCGGCTTTCACATTTTAAACAAAGGACGTTTCCTTCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTc                                                                                   <  2:1675438/144‑1 (MQ=255)
                                                                       tCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGt                                                                             >  1:2005841/1‑151 (MQ=255)
                                                                         cATTGTTAACAACAAAAAACCCGCAGCAGTTGCAGTCGTTTTTCTCATTTTAAAGGAATTCAGTTTCCTTATTTTTACCTGGTTTTTCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCTTTTCAGCGGttt                                                                           <  1:1584669/151‑1 (MQ=255)
                                                                               tAGAAAAAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAAGGAATGACTTTTATTTCTCTTTCCCCTGGTTGGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAt                                                                      <  2:1588959/150‑1 (MQ=255)
                                                                                aGCAAAAAAAAAGCCGACTCCCTTGCAGTCGGTTTTCTCATTTTAAACGAATGACGTTTCCTTCGCTTTACCCTGGTTGGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAtt                                                                     <  2:786057/150‑1 (MQ=255)
                                                                                aGCAAAAAAAAAGCCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAt                                                                      <  2:591885/149‑1 (MQ=255)
                                                                                aGCAAAAAAAAACCCGACTCATTTGCAGTCGGTTTTCTCATTTTAAACGAATGCCGTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAtt                                                                     <  2:411369/150‑1 (MQ=255)
                                                                                 gCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtct                                                                   >  1:1596330/1‑151 (MQ=255)
                                                                                  caacaaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtctc                                                                  >  1:914777/1‑151 (MQ=255)
                                                                                   aacaaAAAAGCCGACTCCCTTGCAGTCGGCTTTCTCATTTTAAACGAATGACTTTTCCTTCGCTTTACCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcg                                                                 <  2:1723473/151‑1 (MQ=255)
                                                                                   aacaaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcg                                                                 >  2:1500224/1‑151 (MQ=255)
                                                                                   aaaaaaaacGCCGCCTCACTTGCAGTCGTTTTTCTCATTTAAAACAAATGACTTTTACTTCGTTTTCCCCTGTTTTACAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtct                                                                   <  1:1742954/146‑1 (MQ=255)
                                                                                   aaaaaaaaaCCCGACTCACTTGCAGTCGGTTTTCTCATTTAAAACGAATGACGTTTCCTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtctc                                                                  <  1:58578/147‑1 (MQ=255)
                                                                                     caaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgtc                                                               >  2:758843/1‑151 (MQ=255)
                                                                                              cGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCAt                                                       >  1:1349051/1‑150 (MQ=255)
                                                                                                 cTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATacac                                                   >  2:1393992/1‑151 (MQ=255)
                                                                                                   ctcTTGCAGGCGGTTTTCTCATTTAAAGCGAGTGCCGTTTCCTTCGCTTTCCCCGTTTTTACAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATacac                                                   <  1:521818/147‑1 (MQ=255)
                                                                                                   cACTTGCGTTCGTTTTTCTCTTTTTAAACAAATACCTTTTCCTTCTTTTTCCCCTGTTTTGCACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATac                                                     <  1:589811/147‑1 (MQ=255)
                                                                                                    aCTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACAcc                                                  >  2:1971682/1‑149 (MQ=255)
                                                                                                    aCTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACAcc                                                  >  1:290701/1‑149 (MQ=255)
                                                                                                      ttGCGGTCGGCTTTCTCATTTAAACGGAATGCCGTTTCCTTCTTTTTCCCCTGGTTGACAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCa                                                 <  1:803833/148‑1 (MQ=255)
                                                                                                         cAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGc                                            >  1:1922436/1‑150 (MQ=255)
                                                                                                          aGTCGTCTTTTTCATTTAAATCGAATATCTTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCa                                           <  1:1612885/150‑1 (MQ=255)
                                                                                                           gTCGGTTTTCTCATTTAAAACGAATGCCTTTTCCTTCGCTTTCCCCTGGTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCAc                                          <  1:870825/150‑1 (MQ=255)
                                                                                                           gTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAACTTCTCGTCGAACTCATACACCAGCGGCACg                                         >  2:1143482/1‑151 (MQ=255)
                                                                                                            tCGGCTTCCTCATTTGAAATGAATGACTTTTTCTTCCCTTTACCCTGATTGGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGc                                               <  2:405849/144‑1 (MQ=255)
                                                                                                              gtttttCTCATTTTAAACGAATGCCTTTTACTCCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCgg                                             <  1:1054820/141‑1 (MQ=255)
                                                                                                              gtttttCTCATTTTAAACGAATGCCGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCAc                                          <  1:378072/144‑1 (MQ=255)
                                                                                                                cTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGt                                    >  1:1932417/1‑151 (MQ=255)
                                                                                                                 tttCTCATTTAAAACAATTGTCTTTTTCTTCTTTTCCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGt                                    <  1:1188224/150‑1 (MQ=255)
                                                                                                                  ttCTTATTTTAAGCGAATGACTTTTCCTCTGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTc                                   <  2:1144954/150‑1 (MQ=255)
                                                                                                                   tctcATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCgg                                 >  1:1387771/1‑151 (MQ=255)
                                                                                                                        tttaAAACGAATGACTTTTCCTTCTTTTTCCCCTGGTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGata                             <  1:1142325/150‑1 (MQ=255)
                                                                                                                        tttaAAACGAATGACGTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGata                             <  1:483729/150‑1 (MQ=255)
                                                                                                                         tttAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATAtt                           >  2:1648175/1‑151 (MQ=255)
                                                                                                                          taaaaCGAATGACTTTTATTTCGCTTTACCCGGTTTGGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATAtt                           <  1:1448381/148‑1 (MQ=255)
                                                                                                                           tAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTa                          >  1:735812/1‑150 (MQ=255)
                                                                                                                           aaaaCGAATCGCGTTTACTTCGCTTCCCCCTGTTTCCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATAtt                           <  1:1425583/148‑1 (MQ=255)
                                                                                                                            aaaCGAATGACGTTTCCTCCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTaa                         <  2:834980/150‑1 (MQ=255)
                                                                                                                            aaaCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCACACACCAGCGGCACGCCAGTCGGGATATTa                          >  2:1737760/1‑149 (MQ=255)
                                                                                                                            aaaCAAATGACTTTTACTTCGCTTTCCCCTGTTTTACACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATAtt                           <  1:1660985/148‑1 (MQ=255)
                                                                                                                             accgaagggcttttCCTCCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGc                       <  1:1645468/140‑1 (MQ=255)
                                                                                                                              aCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCt                      >  2:30191/1‑151 (MQ=255)
                                                                                                                              aCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCt                      >  1:1755558/1‑151 (MQ=255)
                                                                                                                              aCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCt                      >  2:1034442/1‑151 (MQ=255)
                                                                                                                                 aaTGACTTTTCCTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCt                      <  2:914799/148‑1 (MQ=255)
                                                                                                                                 aaTGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa                    >  1:1180275/1‑150 (MQ=255)
                                                                                                                                   tGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa                    >  2:1879182/1‑148 (MQ=255)
                                                                                                                                     aCGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAAt               >  2:1988470/1‑151 (MQ=255)
                                                                                                                                        tttCCTTCGTTTTCCCCGGGTTTGAAGCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAAtct             <  2:2024535/150‑1 (MQ=255)
                                                                                                                                            cTTCGTTTTCCCCTGGTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCtctt          <  1:2051137/149‑1 (MQ=255)
                                                                                                                                            cTTCGCTTTCCCCGGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATctct           <  2:549573/148‑1 (MQ=255)
                                                                                                                                               cgttttCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCttcttc      <  2:766444/147‑1 (MQ=255)
                                                                                                                                                gCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGc    >  2:1406355/1‑151 (MQ=255)
                                                                                                                                                gCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGc    >  1:329138/1‑151 (MQ=255)
                                                                                                                                                  tttACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTc  >  2:1068074/1‑151 (MQ=255)
                                                                                                                                                   ttACCCTGTTTGGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCttcttc      <  2:959652/146‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTC  >  NC_000913/786706‑787002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑