Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA45.8% 135.0 / 95.4 118intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (59/5);  new base A (0/54);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 6.26e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATT  >  NC_000913/29338‑29636
                                                                                                                                                      |                                                                                                                                                      
tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCcaca                                                                                                                                                       <  1:1257646/150‑1 (MQ=255)
tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCcaca                                                                                                                                                       <  2:1049564/150‑1 (MQ=255)
tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCcaca                                                                                                                                                       >  1:1485536/1‑150 (MQ=255)
     tgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAaata                                                                                                                                                  >  1:1919904/1‑150 (MQ=255)
       ttttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAaataat                                                                                                                                                >  1:1815507/1‑150 (MQ=255)
             tGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATaaaa                                                                                                                                          >  1:636226/1‑150 (MQ=255)
               tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                        <  1:1338845/150‑1 (MQ=255)
               tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                       >  1:985068/1‑151 (MQ=255)
                aaaTTTTGGCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                       <  2:2024578/150‑1 (MQ=255)
                 aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                     >  1:750295/1‑151 (MQ=255)
                  aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                    >  1:411928/1‑151 (MQ=255)
                  aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                    <  2:388165/151‑1 (MQ=255)
                     ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                 >  1:1589372/1‑151 (MQ=255)
                          cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCACCATTa                                                                                                                             >  2:496445/1‑150 (MQ=255)
                               ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                        >  2:324678/1‑150 (MQ=255)
                               ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                        >  1:8957/1‑150 (MQ=255)
                                  ccACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                    >  2:253289/1‑151 (MQ=255)
                                  ccACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                    >  2:344523/1‑151 (MQ=255)
                                   cACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                   >  2:715394/1‑151 (MQ=255)
                                   cACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                   >  1:380624/1‑151 (MQ=255)
                                     cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                  >  2:877263/1‑150 (MQ=255)
                                      ttttttCTGCTCTTTTTTATTTCATACATTCTTCTGCCTGCGCAAGCTTTTCCAAGAAAAGGTA‑AAATGATCTTTTTTTCGTTAAATCCTTGAAAAAC‑ATGCAGGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                  <  2:1139723/149‑1 (MQ=255)
                                      ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                  >  1:193429/1‑149 (MQ=255)
                                       tttttCTGCTCTTTTTTTTTTCATGCATTTTTCTTGCTGCGCAAGCTTTTTCAAAAACGGTTT‑AGATGTTTTTTTTGTCGTTTAATGCTTGAAAAAC‑ATGAATGAGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                <  2:168943/150‑1 (MQ=255)
                                        ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                              >  1:208513/1‑151 (MQ=255)
                                        ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                              >  1:480044/1‑151 (MQ=255)
                                         tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                             >  2:1007398/1‑151 (MQ=255)
                                                   tttttATTTCATGCATTTTTTTTGCTGCGCAAGCTTTTCCAAAAAAAGTTT‑AGATGTTTTTTTTGTCGTTTAATCCTTAAAAAAC‑ATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                       <  2:191187/147‑1 (MQ=255)
                                                   tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                   >  1:1269754/1‑151 (MQ=255)
                                                    ttttttttCATGAATTCTTCTTGCGGCGCAGGCGTTTTCAAGAACTGGTT‑AAATTATCTTTTTGCCGCTAAATCCCTAAAAACC‑ATGCATGCCCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                   <  2:713762/150‑1 (MQ=255)
                                                     ttttttTCATGAAATCTTCTTGCTGCGCAAGTTTTTTCAAAAACAGGTT‑AGATAATTTTTTTGTCGTTAAATGCCTAAAAAAC‑ATGCTGGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                                  <  1:243548/150‑1 (MQ=255)
                                                          ttaaTGCATTCTTTTTGCTGCGCAAGCTTTTCCCAGAACAGGTT‑AAATGTTTTTTTTGTCGCTTAAGGCTTGAAAAAC‑ATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                             <  2:851878/147‑1 (MQ=255)
                                                          ttCATGCATTTTTCTGGCTCCCAAGGTTTTTTCCAAAAAGGGTT‑AAATGTTCTTTTTGTCTTTAAATGCCTGAAAAAC‑ATGCAGGACCCAAAAAATATTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                              <  2:461245/149‑1 (MQ=255)
                                                             ataaatttttcttGCTGCGCAACCGTTTTCCAAATTGGATG‑AAAGGATTTTTTTCTCGTATAACGCCAAAAAAAC‑ATGCAGGCGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                          <  2:1471767/142‑1 (MQ=255)
                                                             aTGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgc                                                                                         >  1:165376/1‑151 (MQ=255)
                                                                     ttcttGCTGCGCAAGCTTTTTCAAAAACAGTTT‑AGATGTTTTTTTTGTCGTTAAATGCTTAAAAAAC‑ATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                    <  2:1487392/148‑1 (MQ=255)
                                                                       cttGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                 >  1:503822/1‑149 (MQ=255)
                                                                        ttGCTGCGAAGGCTTTTCCAAGAACAGGTT‑AGATTATTTTTTTTTCGTTAATTGCCTGAAAAAC‑AGGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                       <  2:1740006/142‑1 (MQ=255)
                                                                         tgctgcG‑AAGGCTTTTCAAAAAAGGGTTAAGATGGTTTTTTTGTCATTTAATGCTTATAAAAC‑ATGCATGGGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                               <  2:1142852/149‑1 (MQ=255)
                                                                              cgcATGCGTTTTCCAAAACAGTTT‑AGATGATTTTTTTGTCGTTTAATGCCTGAAAAAC‑ATGCAGGACCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                          <  2:710364/149‑1 (MQ=255)
                                                                                cAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                      >  1:1612518/1‑151 (MQ=255)
                                                                                 aatcttttcCCAGAAAAGTTT‑AAATTTTTTTTTTTTCTCTTAGTCCCTAAAAAAC‑ATTAATGAGCAAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                         <  1:1394923/142‑1 (MQ=255)
                                                                                 aaGCTTTTCCAAAAAAAGGTT‑AGATGTTTTTTTTTTCTTTTAATGCTTATAAAAC‑ATGCATGAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                        <  2:592058/148‑1 (MQ=255)
                                                                                  agacttttCCCAAAAAGGTTAAGATGATTTTTTTGTCGATTATTCCCGGAAAAAC‑TGCCAGGAGCCACAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                      <  2:1271730/146‑1 (MQ=255)
                                                                                     ttttcCCAAAACGGGTT‑AGATTTTTTTTTTGTCCCTTAATCCCTAAAAAAC‑ATGCATGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                   <  1:1475430/148‑1 (MQ=255)
                                                                                      ttttCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAACAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                   >  1:1940193/1‑148 (MQ=255)
                                                                                      ttttCAAAAACGGGTT‑AGATGTTCTTTTTGTCTATAAATCCCTAAAAAAC‑ATGCAGGACCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                  <  2:1403897/149‑1 (MQ=255)
                                                                                      tttccaaaaaCAGGTT‑AGATGTTTTTTTTGTCGTTTAATCCCTGAAAAAC‑ATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                    <  2:497555/139‑1 (MQ=255)
                                                                                      tttccaaaaaCAGGTT‑AGATGTTCTTTTTGTCGTTAAATGCCTAAAAAAC‑ATGCATGGCCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                  <  2:100957/141‑1 (MQ=255)
                                                                                          ccAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                               >  1:868731/1‑148 (MQ=255)
                                                                                          ccAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                               >  2:1360568/1‑148 (MQ=255)
                                                                                            aGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                          >  2:212062/1‑151 (MQ=255)
                                                                                              aaCAGTTT‑GAATGATTTTTTTGTCTTTAAAGGCTTGAAAAAC‑ATGCGTGGGCAAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                         <  2:1890197/150‑1 (MQ=255)
                                                                                              aaCAGTTT‑AGATTATTTTTTTGCCGCTTAATGCCTAAAAAAC‑ATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                         <  2:1269791/150‑1 (MQ=255)
                                                                                                cAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                       >  2:2024129/1‑150 (MQ=255)
                                                                                                    tt‑AGTTTATTTTTTTTTCGCTTAATGCCTGAAAAAT‑AGGCTTGCACCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                    <  2:1470009/149‑1 (MQ=255)
                                                                                                    tt‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                   >  1:222965/1‑150 (MQ=255)
                                                                                                     t‑AGATGATCCTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                 >  1:537882/1‑151 (MQ=255)
                                                                                                       aGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                  <  2:2026120/149‑1 (MQ=255)
                                                                                                         atgatCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                               >  1:578465/1‑150 (MQ=255)
                                                                                                          tgatCTTTTTGTCGTTTAATGCCTGAAAAAC‑AGGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                  <  2:1120438/146‑1 (MQ=255)
                                                                                                           gatCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                            >  2:1278020/1‑151 (MQ=255)
                                                                                                                 tttGTCTTTTAATCCCTGAAAAAC‑ATGCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                       <  1:153384/150‑1 (MQ=255)
                                                                                                                 tttGTCGTTTAAT‑CCTTTAAAACAATCCATGACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                       <  2:1671872/150‑1 (MQ=255)
                                                                                                                    gTCGTTAAATCCTTGAAAAAC‑ATGCAGGAACCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                    <  2:411202/150‑1 (MQ=255)
                                                                                                                       gtttAATGCTTGAAAAAC‑AGGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                <  1:692410/149‑1 (MQ=255)
                                                                                                                         ttAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                              >  2:548913/1‑151 (MQ=255)
                                                                                                                           aatgcgtaaaaaaC‑ATGCAGGCCCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                               <  2:1117245/139‑1 (MQ=255)
                                                                                                                           aaTGCCTGAAAAAC‑ATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                            <  2:189961/151‑1 (MQ=255)
                                                                                                                           aaTGCCTAAAAAAC‑AGTCAGGACCCAAAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                               <  1:1291292/148‑1 (MQ=255)
                                                                                                                            aTGTCTGAAAAAC‑GTGCATGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                              <  1:1099212/148‑1 (MQ=255)
                                                                                                                            aTGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                            >  2:747607/1‑150 (MQ=255)
                                                                                                                            aTGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                            >  1:1127186/1‑150 (MQ=255)
                                                                                                                             tGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                          >  2:805399/1‑151 (MQ=255)
                                                                                                                               cctaaaaaaC‑ATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                        <  2:1736033/146‑1 (MQ=255)
                                                                                                                                 taaaaaaC‑AGGCATGACCCAAAAAAAAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                       <  2:480055/147‑1 (MQ=255)
                                                                                                                                   aaaaaC‑ATGCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                    <  2:136690/150‑1 (MQ=255)
                                                                                                                                   aaaaaC‑ATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                       <  2:2021871/147‑1 (MQ=255)
                                                                                                                                    aaaaC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                    >  1:11277/1‑150 (MQ=255)
                                                                                                                                    aaaaC‑ATGAATGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                     <  2:1905424/149‑1 (MQ=255)
                                                                                                                                    aaaaC‑ATGAATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                      <  2:52610/148‑1 (MQ=255)
                                                                                                                                     aaaC‑ATGAATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                  <  2:1485604/151‑1 (MQ=255)
                                                                                                                                       aatgggaataaGCCAAAAAAAAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                 <  2:382912/141‑1 (MQ=255)
                                                                                                                                         caggcatgACCAAAAAAAAAAAAAAAAAAACACCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa               <  1:1551672/148‑1 (MQ=255)
                                                                                                                                          gtgagggtgCCAAAAAAAAATGAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag              <  2:636242/143‑1 (MQ=255)
                                                                                                                                           tgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt             >  2:1299437/1‑151 (MQ=255)
                                                                                                                                           tgcatgACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa               <  1:76517/149‑1 (MQ=255)
                                                                                                                                           ggcgtgAGCCAAAAAAAAAAAAAAAAAACACCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt             <  1:1759336/147‑1 (MQ=255)
                                                                                                                                            gcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg            >  1:1543358/1‑151 (MQ=255)
                                                                                                                                            gcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg            >  1:608753/1‑151 (MQ=255)
                                                                                                                                             catgACCAAAAAAAAAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                 <  1:1307055/145‑1 (MQ=255)
                                                                                                                                              atgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg            >  2:900557/1‑149 (MQ=255)
                                                                                                                                              atgAGACACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt             >  2:1206740/1‑148 (MQ=255)
                                                                                                                                               agAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa               <  1:28462/144‑1 (MQ=255)
                                                                                                                                                gAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg        >  2:233964/1‑151 (MQ=255)
                                                                                                                                                  cccAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTG‑CCTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg        <  2:1395038/147‑1 (MQ=255)
                                                                                                                                                   ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat     >  1:1837240/1‑151 (MQ=255)
                                                                                                                                                    cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat     >  1:101424/1‑150 (MQ=255)
                                                                                                                                                     acaAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg        <  2:681227/146‑1 (MQ=255)
                                                                                                                                                      caAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACTTCATCATTGTGAATTAATATGCAAATAAAGCGAGTGAATAtt  >  1:1060977/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAAC‑ATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATT  >  NC_000913/29338‑29636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑