Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,621,1180AC40.0% 164.3 / 18.3 96V7G (GTG→GGG) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base A (39/18);  new base C (0/38);  total (39/57)
Fisher's exact test for biased strand distribution p-value = 4.62e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTT  >  NC_000913/1620969‑1621268
                                                                                                                                                       |                                                                                                                                                       
gTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAacac                                                                                                                                                        >  2:1443559/1‑151 (MQ=255)
  aCTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACAccc                                                                                                                                                      >  2:1355574/1‑151 (MQ=255)
      tCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCa                                                                                                                                                    <  1:165075/149‑1 (MQ=255)
        ggTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCtt                                                                                                                                                <  2:1285886/151‑1 (MQ=255)
         gTCGTGCGACAAACAAGATAGCCGGAAAAGCGCCCAGCATAAAGCGCAAACCGGCCAGCATCAGCGTTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTTCCAGTAGCGCCAACACCCCATCttt                                                                                                                                               <  1:2017825/151‑1 (MQ=255)
           cGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCtttt                                                                                                                                              <  2:974337/150‑1 (MQ=255)
           cGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTc                                                                                                                                             <  1:1048066/151‑1 (MQ=255)
                gACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAc                                                                                                                                        >  2:1342606/1‑151 (MQ=255)
                gACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAc                                                                                                                                        <  1:492777/151‑1 (MQ=255)
                 aCAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAc                                                                                                                                        >  2:828326/1‑150 (MQ=255)
                        aGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTAcc                                                                                                                               >  2:1760942/1‑151 (MQ=255)
                        aGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTAcc                                                                                                                               <  1:1190457/151‑1 (MQ=255)
                                ggAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTg                                                                                                                        >  2:1349863/1‑150 (MQ=255)
                                 gAAAAGCGACCAGAATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGCCCACAAAATTTAGCCCCC‑ATACGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTg                                                                                                                        <  1:1353475/149‑1 (MQ=255)
                                  aaaaGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGCCCACTCCCAGTAGCGCCACCCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAAt                                                                                                                     <  2:609884/151‑1 (MQ=255)
                                  aaaaGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAAt                                                                                                                     >  1:1349879/1‑151 (MQ=255)
                                    aaGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGAGGCATGTTATGAATCCCCAC‑TTTGACGACCACAAAATTTAGCCACC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑CTTCTAGCGCCTCTGAAttt                                                                                                                   >  1:481672/1‑151 (MQ=255)
                                      gCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTc                                                                                                                  >  2:1758893/1‑150 (MQ=255)
                                       cGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCAt                                                                                                                >  2:519915/1‑151 (MQ=255)
                                         aCCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCt                                                                                                              >  2:194385/1‑151 (MQ=255)
                                         aCCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCAAAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCAt                                                                                                                <  2:768576/149‑1 (MQ=255)
                                           cAGCATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCCCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCt                                                                                                              <  2:1546607/149‑1 (MQ=255)
                                            agaaaaaaCCGCAAACCGGCCAGCACCAGCGGTGGCATGTTATGAACCCCCAC‑TTTGATGCCCACAAAATTTAGCCCCC‑ATACGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCtttt                                                                                                           <  1:230610/146‑1 (MQ=255)
                                               aaaaaCCGCAACCCGGCCAGCACCGGCGGTGGCATGTTATGAACCCCCCT‑TTTGATGCCCAAAAATTTTACCCCCC‑ATCCGCCCCCTCCCAGTAGCCCCAACCCCCCATTTTTCCGCGAC‑ATTCTACCGCCTCTGAATTTCATCtttt                                                                                                           <  1:382541/146‑1 (MQ=255)
                                               aTAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGt                                                                                                         >  1:1031520/1‑150 (MQ=255)
                                                aaacgcaaaacccGCCCAGCATCACCGGACCCATTTTGCAAACCCCCAC‑TTTAATCACCACAAAATTTAGCCCCC‑ATCCGCCCCCTCCCAGTAGCCCAACCACCCTATCTTTACCCGAA‑ATTCACCCGCCTCTGAATTTCATCTTTTGTaa                                                                                                       <  1:1649228/140‑1 (MQ=255)
                                                     cccAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCCCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAAt                                                                                                  <  2:740074/149‑1 (MQ=255)
                                                     cacAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCAAAAAATTAAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTCCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCaa                                                                                                   <  2:808126/148‑1 (MQ=255)
                                                        aacccGGCCAGCACCAGCGGTGGCATTTTATGAACCCCCTT‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGCCCACTACCAGAAGCCCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCa                                                                                                <  2:1782557/147‑1 (MQ=255)
                                                        aacccGGCCAGAAGCAGCGGTGCCATGTTATGAGCCCCCAC‑TTGCATGCCCAAAAAATTTAGCCCCC‑ATTCGCCCACTCCCATGAGCGCCACCACCCCATCTTTCCGCCAC‑ATCCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATc                                                                                                 <  1:553680/146‑1 (MQ=255)
                                                        aacccGCCCAGCATCAGCGGTGGCATGTTATGAAGCCCCTC‑TTTAAGGACCACAAAATTTAGCCCCC‑ATCCGCCCCCTCCCAGTAGCCCCACCCCCCCATCTTTCCGCGAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATc                                                                                                 <  1:2051510/146‑1 (MQ=255)
                                                          aCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACt                                                                                             >  1:1860794/1‑151 (MQ=255)
                                                            cGGCCAGCATCAGCGGTGGCATGTTAAGAAGCCCCCG‑TTTGATGACCACAAAATTTACCCCCC‑ATACGACCCCTCCCAGAAGCGCCACCCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTa                                                                                           <  1:712755/151‑1 (MQ=255)
                                                             gaccAGAATCGGCGGTGCTATGTTTTGAA‑CCCCACTTTTTAGTTCCACAAAATTAAGCCCCC‑ATCCTTCCACTACCAGTACCAACAACACCCCTTCTTTCCGCAAC‑ATTCCTCCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg                                                                                          <  2:1474723/149‑1 (MQ=255)
                                                              gCCAGCATCGGCGGTGGCATTTAATAAACCCCCCC‑TTAAATGCCCAAAATATTAACCCCCC‑CCCCGCCCCCCACCACTATCCCCAACACCCCATTTTTTCGCGAG‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg                                                                                          <  1:114648/150‑1 (MQ=255)
                                                              gCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGc                                                                                         >  1:1574824/1‑151 (MQ=255)
                                                               ccAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt                                                                                        >  1:862032/1‑151 (MQ=255)
                                                                cAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg                                                                                       >  2:1474076/1‑151 (MQ=255)
                                                                 aGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg                                                                                       >  2:751636/1‑150 (MQ=255)
                                                                   caacAGCGGGGGCATGTTATGAAGCCCCCC‑TTGGATGACCAAAAAATTTACCCCCC‑ATCCGCCCCCTACCAGTAGCCCCAACCCCCCATCTTTTGGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGaa                                                                                     <  2:238322/147‑1 (MQ=255)
                                                                   cATCAGGGGTGGCATTTTATGAAGCCCCAC‑TTGAATGCCCACAAAATTTACCCCCC‑ATACGCCCACTACCAGGAGCGCCACCCCCCCATCTTTCCGCGAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg                                                                                       <  1:1662652/148‑1 (MQ=255)
                                                                     tCAGCGGTGGAATGTTAGGAGGCCCCAC‑TTTGAGTACCAAAAAATTAAGCCCCC‑ACACGACCACTACGAATAGCGCAACCCCCCCATCTTTTGGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGa                                                                                      <  2:989344/147‑1 (MQ=255)
                                                                      cAGCGGTGGCATGTTATGAACCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTGGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAttt                                                                                  >  1:205376/1‑150 (MQ=255)
                                                                       aGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTa                                                                                 >  2:448072/1‑150 (MQ=255)
                                                                       aGCGGTGCCTTGTTATGAAGCCCCCT‑TTTGAGGACCA‑AAAATTTTGCCCCCAATCCGCCCCCTACCAGTAGCGCAAACACCCCATTTTTCCGCGCC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGa                                                                                      <  2:90627/145‑1 (MQ=255)
                                                                        gCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt                                                                               >  2:1986027/1‑151 (MQ=255)
                                                                        gCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCAAAAAATTTAGCCCCC‑ATACGCCCACTCCCAGTAGCCCCACCCCCCCATCTTTTCGCGAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt                                                                               <  2:1039458/151‑1 (MQ=255)
                                                                        gCGGTGGAATTTTATGAACCCCCAT‑TTTGATGCCCAAAAAATTTACCCCCC‑ACACGACCCCTCCCAGTAGCCCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTAc                                                                                <  1:1087320/150‑1 (MQ=255)
                                                                          ggTGGCATTTTATGAACCCCCTC‑TTTGATGCCCAAAAAATTTAGCCCCC‑ATACGCCCCCACCCAGGAGCCCCAACACCCCATCTTTTCGCTAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACttt                                                                             <  1:230957/151‑1 (MQ=255)
                                                                          ggTGGAATTTTAAGAACCCCCAT‑TTTTATGACCAAAAAATTTTCCCCCC‑ATACCACCCCCCCAATTGGCGCCACCACCCCATCTTTCCGCAAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtt                                                                              <  1:1628443/150‑1 (MQ=255)
                                                                           gTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtttt                                                                            >  2:1116751/1‑151 (MQ=255)
                                                                             ggCATGTTAGGAACCCCCCC‑TTTGATGCCCACAAAATTTACCCCCC‑ATACGACCACTCCCAGTGGCCCCAACCCCCCATTTTTTCGCGAT‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtttt                                                                            <  1:1226225/149‑1 (MQ=255)
                                                                                aTGTTTTAAAGCCCCAC‑TTTGATAACAACAAAATTTACCCCCT‑ATCCCCCCCCACCCATTAGCGCAAACCCCCCATCTTTCCGCAAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtttt                                                                            <  1:1674809/146‑1 (MQ=255)
                                                                                 tGTTATGACGCCCCTC‑TTTGATGACCAAAAAATTTAGCCCCC‑ATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCtt                                                                         <  2:1411486/148‑1 (MQ=255)
                                                                                       gaggCCCCGC‑TTTGATGTCCCTAATATTCAGCCCCA‑ATCCGACCCCTCCCTGTAGGGCCACCCCCCCATC‑TTTAGCGGCAATTCTCCCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                                 <  2:585856/147‑1 (MQ=255)
                                                                                        taaccccctatttGATGACCCAAAAATTATGCCCCC‑TTACCCCCCCTACCAATACCGCAAACCCTCAATCTTTCCTCGAA‑TTACTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCtt                                                                         <  1:21202/132‑1 (MQ=255)
                                                                                        aacccccTC‑TTTGATCCCCAAAAAATTTCCCCCCC‑ATACGACCCCTACCAGTAGCCCCACCCCCCCATTTTTTCGCGAT‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                                 <  1:1355531/146‑1 (MQ=255)
                                                                                        aaccccaAC‑TTAGATGCCCAAAAAATGTACCCCCA‑AAACGACCACTACCAGGGGCGCCAACACCCCTTCTTTTCGCGAC‑ATTTTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                                 <  2:524398/146‑1 (MQ=255)
                                                                                           ccccAC‑TTTGATGACCACAAAATTAACCCCCC‑ATACGACCCCTACCAGTAGCGCCACCCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                                 <  2:1987333/146‑1 (MQ=255)
                                                                                                c‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGtt                                                         >  1:1564478/1‑149 (MQ=255)
                                                                                                    tgtggaCCACAAAATTTAGCCCCC‑ATACGCCCACTCCCAGTAGCGCCACCTCCCCTTCTTTTCGCGAC‑ATCCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTc                                                     <  2:827316/146‑1 (MQ=255)
                                                                                                      atgaCCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGcc                                                  >  2:827162/1‑151 (MQ=255)
                                                                                                        gaCCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCgg                                                >  2:1379187/1‑151 (MQ=255)
                                                                                                           cacaAAATTTACCCCCA‑ATACCACCACTACCAGTAGCGCCACCACCCCTTCTTTTCGCATC‑ATTATACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTa                                             <  1:1440698/151‑1 (MQ=255)
                                                                                                           caaaaaaTTTAGCCCCC‑ATACGCCCCCTCCCAGTAGCGCAAACCCCCCATTTTTTCGCAAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGt                                               <  1:1059287/146‑1 (MQ=255)
                                                                                                            acaAAATTTAGCCCCC‑ATCCGACCCCTCCCAGTAGCCCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTac                                            <  1:1830971/151‑1 (MQ=255)
                                                                                                            aaaaaaTTTACCCCCC‑ATACGCCCACTACCAGTAGCGCCACCCCCCCATCTTTCCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGtt                                              <  2:1251534/147‑1 (MQ=255)
                                                                                                               aaaTTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTCGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacga                                          >  1:1312559/1‑150 (MQ=255)
                                                                                                                    tAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAt                                    >  2:1226828/1‑151 (MQ=255)
                                                                                                                      gCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt                                   >  2:1925704/1‑150 (MQ=255)
                                                                                                                       ccccc‑ATCCGCCCCCTCCCATTAGCCCAAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCa                                     <  2:1566085/147‑1 (MQ=255)
                                                                                                                       cccca‑ATACGCCCCCTCCCAGGAGCCCAACCCCCCCATTTTTCCGCGAC‑TTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt                                   <  1:1553494/149‑1 (MQ=255)
                                                                                                                         ccc‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATg                               >  1:164339/1‑151 (MQ=255)
                                                                                                                         ccc‑ATACCACCTCTCCCAGAAGCCCAAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATg                               <  2:2031896/151‑1 (MQ=255)
                                                                                                                           c‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcg                             >  2:1054153/1‑151 (MQ=255)
                                                                                                                               aCGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCa                           >  1:1758125/1‑150 (MQ=255)
                                                                                                                                  aCCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAAt                        >  2:170634/1‑150 (MQ=255)
                                                                                                                                  aCCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAAt                        >  2:912363/1‑150 (MQ=255)
                                                                                                                                   ccccTACCAGTAGCACAACCCCCCCATCTTTTCGCAAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcg                             <  2:525234/141‑1 (MQ=255)
                                                                                                                                   ccACTACCAGTGGCCCCAACCCCCCATTTTTTCGCGAC‑ATTCTCCCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa                       <  2:370214/150‑1 (MQ=255)
                                                                                                                                   ccACTACCAGTAGCGCCAACCCCCCATCTTTCCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa                       <  2:467935/150‑1 (MQ=255)
                                                                                                                                    cACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa                       >  1:2087773/1‑149 (MQ=255)
                                                                                                                                         ccAGTGGCCCCACCCCCCCATTTTTCCGCGAA‑TTTCAACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa                       <  1:128852/144‑1 (MQ=255)
                                                                                                                                         ccAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTc                   <  2:1081203/148‑1 (MQ=255)
                                                                                                                                         ccAGTAGCGCCAACCCCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGc                 <  2:2027236/150‑1 (MQ=255)
                                                                                                                                         ccAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt                >  2:98375/1‑151 (MQ=255)
                                                                                                                                         ccAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt                >  2:1508600/1‑151 (MQ=255)
                                                                                                                                         ccAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt                >  2:2019046/1‑151 (MQ=255)
                                                                                                                                           aGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtat              >  2:941479/1‑151 (MQ=255)
                                                                                                                                           aGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtat              >  1:223734/1‑151 (MQ=255)
                                                                                                                                            gaagtaccAACCCCCCATCTTTGCGCTAT‑TTCCAACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtat              <  1:1032924/144‑1 (MQ=255)
                                                                                                                                                cgcCAACCCCCCATTTTTCCGCTAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACt           <  2:1343400/149‑1 (MQ=255)
                                                                                                                                                cgcCAACCCCCCACCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc         <  1:482272/151‑1 (MQ=255)
                                                                                                                                                cgcCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc         >  1:1733271/1‑151 (MQ=255)
                                                                                                                                                  ccAACCCCCCATTTTTCCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc         <  1:612449/149‑1 (MQ=255)
                                                                                                                                                  ccAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCgg       >  2:2003759/1‑151 (MQ=255)
                                                                                                                                                  ccAACACCCCATCTTCTCGCAAC‑ATACTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACGTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTGAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc         <  1:2022792/149‑1 (MQ=255)
                                                                                                                                                     acacCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAtt    >  1:1792408/1‑151 (MQ=255)
                                                                                                                                                      cacCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAttt   >  1:819128/1‑151 (MQ=255)
                                                                                                                                                       acCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAtttt  >  2:174920/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
GTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCAC‑TTTGATGACCACAAAATTTAGCCCCC‑ATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAC‑ATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTT  >  NC_000913/1620969‑1621268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑