Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG42.6% 140.6 / 41.8 101G337G (GGA→GGG) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (6/52);  new base G (43/0);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 2.11e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTG  >  NC_000913/213194‑213488
                                                                                                                                                   |                                                                                                                                                     
tGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAg                                                                                                                                                     >  2:32463/1‑150 (MQ=255)
  ccTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgtgt                                                                                                                                                  <  2:421986/151‑1 (MQ=255)
    tCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAg                                                                                                                                                     >  1:1241319/1‑146 (MQ=255)
     cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAggg                                                                                                                                                   >  1:1261510/1‑145 (MQ=255)
         cTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACCGCtt                                                                                                                                           >  2:1633496/1‑151 (MQ=255)
          tGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGcct                                                                                                                                           >  2:1899893/1‑148 (MQ=255)
             ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAt                                                                                                                                        <  1:763612/150‑1 (MQ=255)
               aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGGGGGGACAGCTTaa                                                                                                                                         >  1:726211/1‑147 (MQ=255)
               aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCCTaaaggg                                                                                                                                     >  2:1379155/1‑147 (MQ=255)
                cGTTTGGGCGCGCTTGAAATCCGACGCTATCAGTCGCAACTGCGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCAGGGGAggggtaaatctaa                                                                                                                                         >  1:1879584/1‑134 (MQ=255)
                 gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGCTTTATTCggg                                                                                                                                   >  1:688946/1‑151 (MQ=255)
                 gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTGCAGCTTAAATCggg                                                                                                                                   >  1:998814/1‑151 (MQ=255)
                 gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCggg                                                                                                                                   <  1:1956543/151‑1 (MQ=255)
                 gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCggg                                                                                                                                   >  2:846753/1‑151 (MQ=255)
                      ggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGTAAAACtttaaatgggggggg                                                                                                                              >  2:838101/1‑138 (MQ=255)
                       gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAAATTAATGCgggggggg                                                                                                                              >  2:1856014/1‑148 (MQ=255)
                        cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTAGGGAGGGGACCACCTAATGCGGGAgg                                                                                                                                >  2:1200606/1‑147 (MQ=255)
                         gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGata                                                                                                                           <  2:716357/151‑1 (MQ=255)
                              tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGACAACATTGTGCCGTGGCAGACGTGGCTTCAAATGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTc                                                                                                                        <  2:1407379/149‑1 (MQ=255)
                              tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCg                                                                                                                       <  1:1744956/150‑1 (MQ=255)
                               gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGc                                                                                                                      <  1:790913/150‑1 (MQ=255)
                                     cGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATTCGGGGGGGGGAATTTGcccccc                                                                                                                 >  2:491150/1‑149 (MQ=255)
                                      gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTTATACGGGGGGGGGTATTTGcccccccc                                                                                                               >  2:2055942/1‑148 (MQ=255)
                                      gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgcg                                                                                                              <  2:1350279/151‑1 (MQ=255)
                                       aCGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGt                                                                                                             <  2:355130/151‑1 (MQ=255)
                                         gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGCTTAATGCGGGGGGGGGTATTTGcccccc                                                                                                                 >  2:1391997/1‑145 (MQ=255)
                                         gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGAAAGCCTAATTCGGGGGGGGGTATTTGcccccccc                                                                                                               >  2:889917/1‑145 (MQ=255)
                                          cTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGTGGTGGGGGGGGGAAAGCTTTATCTGGGAGGGGATTGTTGCCCTcccc                                                                                                               >  1:220641/1‑144 (MQ=255)
                                            aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAg                                                                                                         <  1:1596083/150‑1 (MQ=255)
                                            aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGa                                                                                                        <  1:1130702/151‑1 (MQ=255)
                                             tCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGGGGGGAAAGCGTTATGCGGGAGGGGGAAATCGCCCCCcgcccgc                                                                                                           >  1:17057/1‑143 (MQ=255)
                                              cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACg                                                                                                      <  2:1587470/151‑1 (MQ=255)
                                               aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGCTTTATGCGGGGGGGGGTATTTGGCCTCcccggg                                                                                                            >  2:1097249/1‑142 (MQ=255)
                                                gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAAAGCGGGGGGCGGTATTTGCCCCCcgcgcggcgacg                                                                                                      >  1:2073174/1‑141 (MQ=255)
                                                   gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGTAAAGCCTAAAGCGGGGGGGGAGATTCGCCCTCCCCGGGCAGACGAAGCg                                                                                                 >  2:1859317/1‑151 (MQ=255)
                                                    cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATGGGGGGGGCGATATTCGCCCCCCGCGTGCAGACGGAGAgg                                                                                                >  2:1982481/1‑151 (MQ=255)
                                                    cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAAGGTGAAACGTAAAAAGGGGGGCGATATTTGCCCCCCCCGTGCCGGCGAAGCgg                                                                                                >  1:2003075/1‑151 (MQ=255)
                                                           ggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑g                                                                                         <  2:1764799/150‑1 (MQ=255)
                                                            gtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑gtg                                                                                       <  2:796394/151‑1 (MQ=255)
                                                              ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATGTTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑gtgc                                                                                      <  1:1129720/150‑1 (MQ=255)
                                                               gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑gtgc‑gt                                                                                   <  1:1199273/151‑1 (MQ=255)
                                                                aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑Gtt                                                                                  <  1:344708/151‑1 (MQ=255)
                                                                aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑Gtt                                                                                  <  1:1856474/151‑1 (MQ=255)
                                                                    aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCaa                                                                              <  2:494263/151‑1 (MQ=255)
                                                                     aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGGGTAAAACTTAATTCGGGGGGGGGTATTTGCCCCCCCCCCGGCGACGAAGGGGGCAGG‑GGGC‑Ggtttta                                                                              >  1:1305406/1‑147 (MQ=255)
                                                                     aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCaaa                                                                             <  2:1800722/151‑1 (MQ=255)
                                                                        cGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGGGGTATTCGCCCTCCCCGTGCAGACGAAGCGGGCAGC‑GGGG‑GGTTCaaa                                                                             >  2:642460/1‑148 (MQ=255)
                                                                        cGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAgcg                                                                          <  2:31600/151‑1 (MQ=255)
                                                                         gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTTAAACGGGGGGGGAAATTTTCCCTCCCCCGGCCGACGGAACGGGCAGC‑GGGC‑GGGTCaaa                                                                             >  1:1279023/1‑147 (MQ=255)
                                                                           cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTTATGCGGGGGGGGATATTTGCCCCCCGCGGGGAGAAGAAACGGGCCGCGGGGG‑GTTTCaaacgcc                                                                         >  1:1003313/1‑146 (MQ=255)
                                                                               gggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAAAGCGGGGGGGGGTATTTGCCCCCCCCGGGCAGACGAAGCGGGCAGC‑GGGC‑GGTTTAAAGCGcc                                                                        >  1:259451/1‑146 (MQ=255)
                                                                               gggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGAt                                                                   <  1:234104/151‑1 (MQ=255)
                                                                                  cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAACTTAATTCGGGAGGGGGTATTTGCCCCCCGCGTGCCGACGAAGCGGGCAGC‑GTGC‑GGTTCCAAACGCCAGGGTtg                                                                 >  2:1598981/1‑150 (MQ=255)
                                                                                  cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCGATATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGC‑GGGC‑GGTTTAAAACGCCCGGGtt                                                                  >  2:573388/1‑149 (MQ=255)
                                                                                  cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgc                                                                <  1:350749/151‑1 (MQ=255)
                                                                                   aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgct                                                               <  2:1382813/151‑1 (MQ=255)
                                                                                    aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGGAGGTACAGCTTAAAGCGGGGGGCGGGATTTGCCCCCCCCGGGCAGACGAAACGGGGAGC‑GTGC‑GGTTTAAAACGCCAGGGGtgct                                                               >  1:2038239/1‑150 (MQ=255)
                                                                                    aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgctg                                                              <  2:109360/151‑1 (MQ=255)
                                                                                    aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgctg                                                              <  1:935760/151‑1 (MQ=255)
                                                                                    aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCCTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgctg                                                              <  1:1822134/151‑1 (MQ=255)
                                                                                     aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTGCAGCTTTATTCGGGGGGCGGTATTCTCCCCCCCCGGGCAGAAGAAGAGGGCAGC‑GGGG‑GGTTCAAAACGCCCggggt                                                                  >  1:831158/1‑143 (MQ=255)
                                                                                     aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGGCGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgctgc                                                             <  2:1960278/151‑1 (MQ=255)
                                                                                        gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCata                                                          <  2:335305/151‑1 (MQ=255)
                                                                                              aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCg                                                    <  2:1218846/151‑1 (MQ=255)
                                                                                               ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGGCTCATGCGGGGGGCGATATTCCGCCTCCCCCTGCAGAAGAAGGGGGGCGC‑GTGC‑GGTTCCAACCCCCCGGATTGGCGCCAATTGTCgg                                                   >  1:672665/1‑151 (MQ=255)
                                                                                               ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGGGGCGATATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGC‑GGGCGGTTTCAAA‑CGCCAGGGTTGCTGCATATTGTCgg                                                   >  2:1507164/1‑151 (MQ=255)
                                                                                                 gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                 <  2:237531/151‑1 (MQ=255)
                                                                                                 gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                 <  1:2091407/151‑1 (MQ=255)
                                                                                                  tgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGGGGACCGCTTTATGCGGGGGGGGGGATTTGCCCCCCGCGTGCCGACGAAGCGGGCAGC‑GGGC‑GGTTTTAAACGCCAGGGGTGCTGCCTATTGTTggggg                                                >  1:548272/1‑149 (MQ=255)
                                                                                                    ccGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGCGGGGGGGGGTATTCGCCCCCCCCGTGCAGACGAAGCGGGCAGC‑GGGC‑GGTTTAAAGCGCCAGGATTGCTGCATATTTTCGGGCg                                                >  2:880574/1‑149 (MQ=255)
                                                                                                     cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTaa                                             <  2:858195/151‑1 (MQ=255)
                                                                                                     cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTaa                                             <  1:1612184/151‑1 (MQ=255)
                                                                                                      gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTGAc                                            <  1:213485/151‑1 (MQ=255)
                                                                                                       tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAATGCGGGGGGGGATATTTGCCCTCCGCGTGCAGACGAAGAGGGCAGC‑GGGC‑GGTTCAAAAGGCCAGGATTGCTGCATATTGTCGGGCGGaa                                             >  2:908529/1‑149 (MQ=255)
                                                                                                       tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGACAACCTAATTCGGGGGGGGATATTCGCCCCCCCCGTGGAGAACAAACGGGGCGG‑GGGG‑GTTTTAAAGAGCCCGGGTTGCTGCATAATGTTggg                                                  >  1:547043/1‑144 (MQ=255)
                                                                                                        ggCAGACGTGGCTTCAACCGCTGGAACTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAACCGCCAGGATTGCTGCATATTGTCGGGCGTAAcg                                           <  1:36794/150‑1 (MQ=255)
                                                                                                         gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGATATTCGCCCCCCCCGGGCAGACGAAGCGGGCAGC‑GGGCGGTTTCAAA‑CGCCAGGAATGCTGCATATTGTCGGGGGTAAagg                                          >  1:791239/1‑150 (MQ=255)
                                                                                                             aCGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGAc                                     <  2:16172/151‑1 (MQ=255)
                                                                                                               gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt                                   <  2:972405/151‑1 (MQ=255)
                                                                                                                 ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTAAAGCCTAATGCGGGGGGCGATATTCGCCCTCCGCGGGCAGAAGGAACGGGCAGC‑GGGC‑GGTTCAAAGCGCCAGGATTGCTGCCTATTGTCGGGCGGAACGGGGGGCg                                    >  2:1171839/1‑148 (MQ=255)
                                                                                                                 ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTaa                                 <  2:512315/151‑1 (MQ=255)
                                                                                                                 ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTaa                                 <  1:1367997/151‑1 (MQ=255)
                                                                                                                 ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTa                                  <  2:1863841/150‑1 (MQ=255)
                                                                                                                  gCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCCATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAg                                <  2:275757/151‑1 (MQ=255)
                                                                                                                      cAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaa                            <  2:1682854/151‑1 (MQ=255)
                                                                                                                       aaCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaaa                           <  2:722398/151‑1 (MQ=255)
                                                                                                                        aCCGCTGGAATTACCGGCGGGGCTGGGGAGTGTAAACCTTAATACCGGGGGCGATATTCTCCCCCCCCCCGGAGACGAAACGGGGAGC‑GGGG‑GTTTTAAAAAGCCAGGATTGCTGCATATTTTCGGGCGGAAaggcggggggtaacca                             >  1:1708570/1‑138 (MQ=255)
                                                                                                                        aCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAg                          <  1:2044725/151‑1 (MQ=255)
                                                                                                                                   tACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGc                <  2:785870/150‑1 (MQ=255)
                                                                                                                                    aCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCaa              <  1:325790/151‑1 (MQ=255)
                                                                                                                                    aCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCa               <  2:505598/150‑1 (MQ=255)
                                                                                                                                       ggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAagag           <  2:1314170/151‑1 (MQ=255)
                                                                                                                                        gcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc          >  1:459296/1‑151 (MQ=255)
                                                                                                                                           gggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg       <  1:278942/151‑1 (MQ=255)
                                                                                                                                           gggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGATAAAGAAAATCTGGCAAGAACt         >  1:1564031/1‑149 (MQ=255)
                                                                                                                                              cTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg    >  1:259003/1‑151 (MQ=255)
                                                                                                                                               tGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg    >  2:273667/1‑150 (MQ=255)
                                                                                                                                                gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGt   <  2:949070/150‑1 (MQ=255)
                                                                                                                                                gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg  >  1:173669/1‑151 (MQ=255)
                                                                                                                                                 ggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg    <  2:556344/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTG  >  NC_000913/213194‑213488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑