Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,377,173 A→C F83C (TTC→TGC)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,1730AC99.1% 373.6 / NA 111F83C (TTC→TGC) sspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (47/63);  total (48/63)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 112 total aligned reads displayed.

CAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAA  >  NC_000913/3377025‑3377315
                                                                                                                                                    |                                                                                                                                              
 aGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAAc                                                                                                                                             <  2:739228/151‑1 (MQ=255)
  gTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACg                                                                                                                                            <  1:237406/151‑1 (MQ=255)
  gTTGCTTACGGGCGGTATCTGCTTCAGAAGTTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTGCAGACCGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAAc                                                                                                                                             <  1:1561369/150‑1 (MQ=255)
       ttACGAGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATc                                                                                                                                      >  1:992238/1‑151 (MQ=255)
         aCGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATcc                                                                                                                                     <  2:946824/151‑1 (MQ=255)
         aCGTCCGGCAGCTGCTTCAGATGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCGTGTACATACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGTGGGCAACGCTCATcc                                                                                                                                     <  1:1622986/151‑1 (MQ=255)
          cGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCa                                                                                                                                    <  2:832188/151‑1 (MQ=255)
          cGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCa                                                                                                                                    <  1:722563/151‑1 (MQ=255)
           gTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATcc                                                                                                                                     >  2:1061439/1‑149 (MQ=255)
           gTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAg                                                                                                                                   <  1:588525/151‑1 (MQ=255)
             gCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGat                                                                                                                                 <  2:254623/151‑1 (MQ=255)
               ggCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGatat                                                                                                                               <  2:1206919/151‑1 (MQ=255)
                gCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAAATAtt                                                                                                                              >  1:1916876/1‑151 (MQ=255)
                   tCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTcc                                                                                                                            >  2:1489085/1‑150 (MQ=255)
                      gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCa                                                                                                                           <  2:880347/148‑1 (MQ=255)
                      gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATaa                                                                                                                        <  1:410432/151‑1 (MQ=255)
                      gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGAAGCGGGCAACggccctcccg                                                                                                                                   >  1:2070696/1‑131 (MQ=255)
                          cAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAatgat                                                                                                                    <  1:1945574/151‑1 (MQ=255)
                           aGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAatga                                                                                                                     >  1:782034/1‑149 (MQ=255)
                             aaGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCg                                                                                                                 <  2:439830/151‑1 (MQ=255)
                               gCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCgag                                                                                                               <  1:1555693/151‑1 (MQ=255)
                                cTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCg                                                                                                                 >  1:824732/1‑148 (MQ=255)
                                   aaCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAGATATTCCATAATTATGCGAGAt                                                                                                             >  1:563702/1‑149 (MQ=255)
                                    aCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCTGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTcc                                                                                                          <  2:1940831/151‑1 (MQ=255)
                                     ccGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTccc                                                                                                         >  1:1178371/1‑151 (MQ=255)
                                      cGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCca                                                                                                        <  1:118763/151‑1 (MQ=255)
                                      cGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCca                                                                                                        <  2:1684953/151‑1 (MQ=255)
                                      cGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCca                                                                                                        <  1:554841/151‑1 (MQ=255)
                                          gatgatGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCCTCCAGATATTCCATAATGATGCGAGATTTCCACAgg                                                                                                    >  1:1269094/1‑151 (MQ=255)
                                             gatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTc                                                                                                 <  1:932239/151‑1 (MQ=255)
                                             gatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTc                                                                                                 <  2:1496704/151‑1 (MQ=255)
                                              atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAggg                                                                                                   >  1:1946828/1‑148 (MQ=255)
                                              atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTc                                                                                                 >  2:1449394/1‑150 (MQ=255)
                                              atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCa                                                                                                <  1:1282876/151‑1 (MQ=255)
                                              atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCa                                                                                                <  1:1439254/151‑1 (MQ=255)
                                                ggTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGc                                                                                              <  2:321656/151‑1 (MQ=255)
                                                 gtgtTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCt                                                                                             <  2:573066/151‑1 (MQ=255)
                                                   gtTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTc                                                                                            <  2:1413404/150‑1 (MQ=255)
                                                     tcatcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAAAGATGCGAGATTCCCACAGGGTCAGCTCACg                                                                                         >  2:1657037/1‑151 (MQ=255)
                                                     tcatcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAAAGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCAc                                                                                          >  2:78346/1‑150 (MQ=255)
                                                       atcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGAt                                                                                       <  2:1756506/151‑1 (MQ=255)
                                                          aGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATcc                                                                                     >  2:1100385/1‑150 (MQ=255)
                                                          aGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGAATCCCACAGGGTCAGCTCACGATcc                                                                                     >  2:1274948/1‑150 (MQ=255)
                                                           gCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccac                                                                                   >  1:1739337/1‑151 (MQ=255)
                                                             gtgtACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccacca                                                                                 <  1:1101545/151‑1 (MQ=255)
                                                                 aCCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAggg                                                                              <  1:155031/150‑1 (MQ=255)
                                                                  ccAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGt                                                                             >  2:117640/1‑150 (MQ=255)
                                                                        tttttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACCCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACCATCCACCAGGGTCGGAACg                                                                      >  2:1016233/1‑151 (MQ=255)
                                                                          tttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGc                                                                     <  1:1201539/150‑1 (MQ=255)
                                                                           ttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGct                                                                    <  2:1516690/150‑1 (MQ=255)
                                                                           ttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTAAGCTCACGATCCACCAGGGTCGGAACGct                                                                    >  2:1181534/1‑150 (MQ=255)
                                                                            tCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGctct                                                                  >  2:608724/1‑151 (MQ=255)
                                                                            tCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGctc                                                                   <  1:1282455/150‑1 (MQ=255)
                                                                             cgatgcgatgCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCCTAATGATGCGAGATTTCCACAAGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTg                                                                 >  1:222189/1‑151 (MQ=255)
                                                                              gatgcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGa                                                                <  2:1447798/151‑1 (MQ=255)
                                                                                 gcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTc                                                             <  1:902861/151‑1 (MQ=255)
                                                                                 gcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTc                                                             <  1:393299/151‑1 (MQ=255)
                                                                                 gcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAGATATTCCATAATGATGCGAGAATCCCACAGGGGCAGCTCACGCTCCACCAGGGTCGGAACGCTCTGGtt                                                              >  1:834283/1‑150 (MQ=255)
                                                                                       cATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGtt                                                        >  2:1559904/1‑150 (MQ=255)
                                                                                        aTGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCCCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGACGGAACGCTCTGATTCGGGtt                                                        >  1:492517/1‑149 (MQ=255)
                                                                                          gTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAg                                                     <  1:1489851/150‑1 (MQ=255)
                                                                                           tACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAgg                                                    >  2:539178/1‑150 (MQ=255)
                                                                                            aCAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTc                                                  <  2:629587/151‑1 (MQ=255)
                                                                                            aCAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTc                                                  <  1:901274/151‑1 (MQ=255)
                                                                                            aCAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTc                                                  >  2:2002231/1‑151 (MQ=255)
                                                                                            aCAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTc                                                  >  1:1879456/1‑151 (MQ=255)
                                                                                            aCAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACCCTCATTCAGATATTTCCTAATGATGCGAGATTCCCCCAGGGCCCGCTCACGGTCCACCCGGGTCGGAACGCTCTGATTTGGGTTTAgg                                                    >  1:1389154/1‑149 (MQ=255)
                                                                                               gACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAAt                                               <  2:1768544/151‑1 (MQ=255)
                                                                                                aCGGCTTTTACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGACGCGGGAAACCCTCATCCAGATATTCCAAAATAATGCGAGATTCCCACAGGGGCAGCTCACGATCCACCCGGGTCGGAACGCCCTGATTCGGGTTGAGGCCAATc                                              >  1:1449615/1‑151 (MQ=255)
                                                                                                 cGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCa                                             <  2:125176/151‑1 (MQ=255)
                                                                                                      ttCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                        >  2:1313429/1‑151 (MQ=255)
                                                                                                       tCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                       >  2:658018/1‑151 (MQ=255)
                                                                                                         aCCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                     <  1:670608/151‑1 (MQ=255)
                                                                                                          ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                    <  1:757329/151‑1 (MQ=255)
                                                                                                          ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                    <  2:249451/151‑1 (MQ=255)
                                                                                                             cgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCg                                 <  1:860612/151‑1 (MQ=255)
                                                                                                             cgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCg                                 >  2:1597685/1‑151 (MQ=255)
                                                                                                              gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                >  2:551609/1‑151 (MQ=255)
                                                                                                                  tACCGGGTAAACAGGCATCAGTGGCGGTTGAGGGCAAGGCTCATCCAGATATTCCATAAAGATGCGAGACTACCACAGGGCCAGCTCAAGATCCACCAGGGTTGGAACGCTCTGATTAGGGCTGCGGTAAATCAGATCCTGAGGCGGAt                              >  2:364500/1‑149 (MQ=255)
                                                                                                                   aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                             <  2:67413/149‑1 (MQ=255)
                                                                                                                    ccGGGTAAACAGGCAGCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                             <  2:1449663/148‑1 (MQ=255)
                                                                                                                     cGGGTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGt                           >  2:255806/1‑149 (MQ=255)
                                                                                                                      gggTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCCGAGGCGGATTGTcc                         >  1:548792/1‑150 (MQ=255)
                                                                                                                        gTAAACAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCttt                      <  1:725495/151‑1 (MQ=255)
                                                                                                                          aaaCAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCtttt                     <  2:649878/150‑1 (MQ=255)
                                                                                                                            aCAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCTCCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                  <  1:1904878/151‑1 (MQ=255)
                                                                                                                            aCAGGCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                  <  1:1998958/151‑1 (MQ=255)
                                                                                                                               ggCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgt               <  2:1916971/151‑1 (MQ=255)
                                                                                                                                gCATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCATgt               >  1:1337380/1‑150 (MQ=255)
                                                                                                                                 cATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtgt             <  1:941070/151‑1 (MQ=255)
                                                                                                                                 cATCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtgt             <  2:1253544/151‑1 (MQ=255)
                                                                                                                                  aTCAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt            >  2:859819/1‑151 (MQ=255)
                                                                                                                                    cAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCg          >  2:1781212/1‑151 (MQ=255)
                                                                                                                                    cAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCg          <  2:1607827/151‑1 (MQ=255)
                                                                                                                                    cAGTGGCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCg          <  2:1371644/151‑1 (MQ=255)
                                                                                                                                        ggCGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtct      >  1:2020187/1‑151 (MQ=255)
                                                                                                                                          cGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg    >  1:1540639/1‑151 (MQ=255)
                                                                                                                                          cGGATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg    <  2:1381320/151‑1 (MQ=255)
                                                                                                                                           ggATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGAAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg    >  2:862642/1‑150 (MQ=255)
                                                                                                                                            gATGCGGGCAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGaa  <  2:750756/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAA  >  NC_000913/3377025‑3377315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: