Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,104,077 (T)8→7 coding (433/1119 nt) wbbK ← lipopolysaccharide biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,104,0700T.98.8% 389.2 / ‑2.7 81coding (440/1119 nt)wbbKlipopolysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (49/31);  total (49/32)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACA  >  NC_000913/2103921‑2104216
                                                                                                                                                     |                                                                                                                                                  
aaGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAt                                                                                                                                                    <  1:75367/150‑1 (MQ=255)
    gTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAtttttt                                                                                                                                               <  1:189761/151‑1 (MQ=255)
       aTTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAttttttta                                                                                                                                             >  1:522851/1‑149 (MQ=255)
         tCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAtttttttaat                                                                                                                                           <  1:446215/150‑4 (MQ=255)
            tAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATg                                                                                                                                         <  2:876340/149‑1 (MQ=255)
            tAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGttt                                                                                                                                      >  2:384035/1‑151 (MQ=255)
            tAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGt                                                                                                                                        <  1:757930/149‑1 (MQ=255)
             aaaTTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGttt                                                                                                                                      >  2:291467/1‑150 (MQ=255)
               aTTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGtttatt                                                                                                                                   <  1:761761/151‑1 (MQ=255)
                    gaATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTtata                                                                                                                              <  1:427324/151‑1 (MQ=255)
                      atcatcatcAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTtatata                                                                                                                            <  1:1037849/151‑1 (MQ=255)
                       tcatcatcaGTAAGTTGGCTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATc                                                                                                                          >  2:1044650/1‑151 (MQ=255)
                         atcatcaGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATc                                                                                                                          >  1:956632/1‑150 (MQ=255)
                         atcatcaGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCa                                                                                                                         <  2:370061/151‑1 (MQ=255)
                           catcaGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAg                                                                                                                        <  2:1075602/150‑1 (MQ=255)
                           catcaGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGc                                                                                                                       <  1:27608/151‑1 (MQ=255)
                           catcaGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGc                                                                                                                       >  2:405385/1‑151 (MQ=255)
                                gTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGta                                                                                                                  <  1:56233/151‑1 (MQ=255)
                                gTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGt                                                                                                                   >  2:543382/1‑150 (MQ=255)
                                      tGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCAt                                                                                                            >  1:198092/1‑151 (MQ=255)
                                            ttATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTaa                                                                                                       >  1:406337/1‑150 (MQ=255)
                                            ttATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTaa                                                                                                       <  2:599990/150‑1 (MQ=255)
                                              aTCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAAt                                                                                                     <  2:761564/150‑1 (MQ=255)
                                                cAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATaa                                                                                                   <  1:53004/150‑1 (MQ=255)
                                                 aGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATa                                                                                                    >  1:331984/1‑148 (MQ=255)
                                                 aGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATa                                                                                                    >  1:922279/1‑148 (MQ=255)
                                                     aatttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGaaaa                                                                                              <  1:106932/150‑1 (MQ=255)
                                                     aatttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGaaaa                                                                                              <  2:76006/150‑1 (MQ=255)
                                                       tttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGc                                                                                           >  1:104400/1‑151 (MQ=255)
                                                       tttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGc                                                                                           >  1:514608/1‑151 (MQ=255)
                                                          aatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTa                                                                                         <  2:618576/150‑1 (MQ=255)
                                                          aatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAg                                                                                        <  2:223475/151‑1 (MQ=255)
                                                           atttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAgg                                                                                       >  2:261235/1‑151 (MQ=255)
                                                             ttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCt                                                                                     >  2:283589/1‑151 (MQ=255)
                                                              tCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCt                                                                                     <  1:145224/150‑1 (MQ=255)
                                                               cTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTcc                                                                                   >  2:154756/1‑151 (MQ=255)
                                                                 ggCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCAt                                                                                 <  1:483357/151‑1 (MQ=255)
                                                                     gACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATa                                                                                >  2:967848/1‑148 (MQ=255)
                                                                        tGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAAtt                                                                          >  1:109403/1‑151 (MQ=255)
                                                                         gACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAAtt                                                                          <  2:1094929/150‑1 (MQ=255)
                                                                          aCAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGAATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTc                                                                        >  1:1096487/1‑151 (MQ=255)
                                                                           cAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTc                                                                        >  2:146961/1‑150 (MQ=255)
                                                                            aaTGTTGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCAc                                                                      >  1:548612/1‑151 (MQ=255)
                                                                            aaTGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCAc                                                                      <  1:371687/151‑1 (MQ=255)
                                                                            aaTGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCAc                                                                      >  2:1155025/1‑151 (MQ=255)
                                                                            aaTGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCAc                                                                      >  2:612374/1‑151 (MQ=255)
                                                                               gatgTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACgg                                                                    <  2:121844/150‑1 (MQ=255)
                                                                                 tgTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAAt                                                                 >  2:883909/1‑151 (MQ=255)
                                                                                  gTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATa                                                                <  1:284961/151‑1 (MQ=255)
                                                                                  gTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATa                                                                <  2:715575/151‑1 (MQ=255)
                                                                                     atttatAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATaaa                                                              >  1:783311/1‑150 (MQ=255)
                                                                                     atttatAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATaaa                                                              <  1:106552/150‑1 (MQ=255)
                                                                                        tataGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTc                                                          >  1:629922/1‑151 (MQ=255)
                                                                                             aaTATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCttt                                                      >  2:711073/1‑150 (MQ=255)
                                                                                             aaTATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCttt                                                      >  1:440925/1‑150 (MQ=255)
                                                                                               tatTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTtat                                                    >  2:744656/1‑150 (MQ=255)
                                                                                                  ttCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATaaa                                                 >  1:1171201/1‑150 (MQ=255)
                                                                                                     ttGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAgg                                             >  1:570693/1‑151 (MQ=255)
                                                                                                      tGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGt                                            >  2:767777/1‑151 (MQ=255)
                                                                                                       gATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTg                                           >  1:245505/1‑151 (MQ=255)
                                                                                                       gATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTg                                           >  2:385296/1‑151 (MQ=255)
                                                                                                          aaaTTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCa                                         <  1:692892/150‑1 (MQ=255)
                                                                                                           aaTTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAgg                                       <  1:384048/151‑1 (MQ=255)
                                                                                                           aaTTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAgg                                       <  2:85275/151‑1 (MQ=255)
                                                                                                            aTTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAg                                        >  1:812579/1‑149 (MQ=255)
                                                                                                              ttttCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGtt                                    >  2:623067/1‑151 (MQ=255)
                                                                                                                   tttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGa                                >  2:518958/1‑150 (MQ=255)
                                                                                                                    ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGa                                <  1:65696/149‑1 (MQ=255)
                                                                                                                    ttcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGAc                               >  1:170665/1‑150 (MQ=255)
                                                                                                                     tcATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACa                              >  2:1392/1‑150 (MQ=255)
                                                                                                                       aTCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATa                           <  1:711930/151‑1 (MQ=255)
                                                                                                                          cAGAATTGTTGAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATaca                         >  1:777604/1‑150 (MQ=255)
                                                                                                                               ttgttgAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATc                   >  2:536947/1‑151 (MQ=255)
                                                                                                                                 gttgAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCtt                 >  2:400172/1‑151 (MQ=255)
                                                                                                                                  ttgAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCttt                >  2:747511/1‑151 (MQ=255)
                                                                                                                                   tgAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCtttt               >  1:45809/1‑151 (MQ=255)
                                                                                                                                    gAACAAACACTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCtttt               <  1:667847/150‑1 (MQ=255)
                                                                                                                                          acacTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAg          >  2:718885/1‑149 (MQ=255)
                                                                                                                                          acacTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGt         >  2:985666/1‑150 (MQ=255)
                                                                                                                                          acacTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTg        <  1:893427/151‑1 (MQ=255)
                                                                                                                                          acacTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTg        >  2:320168/1‑151 (MQ=255)
                                                                                                                                          acacTGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTg        >  1:225509/1‑151 (MQ=255)
                                                                                                                                              tGCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTgacg     >  1:391917/1‑150 (MQ=255)
                                                                                                                                               gCAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTgacgac   >  1:610737/1‑151 (MQ=255)
                                                                                                                                                cAGTATTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACa  <  2:1036566/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACA  >  NC_000913/2103921‑2104216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: