Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	46.5%	72.5 / 55.8	72	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (30/8);  new base A (0/33);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 5.27e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGG  >  NC_000913/29348‑29646                                                                                                                                                      |                                                                                                                                                       tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                       >  1:189318/1‑150 (MQ=255)  aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                      <  2:902213/150‑1 (MQ=255)  aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                      <  2:767376/150‑1 (MQ=255)    tGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                    >  1:526626/1‑150 (MQ=255)       aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                                  >  2:841877/1‑149 (MQ=255)         aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                              <  2:511314/151‑1 (MQ=255)           tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                             <  1:508135/150‑1 (MQ=255)             tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                           >  1:785955/1‑150 (MQ=255)              gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                          <  2:817317/150‑1 (MQ=255)                ccATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                        >  1:649113/1‑150 (MQ=255)                      gttccttttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCGGCACAAGCGTTTTCCAGAAAGGTTT‑AGATGTTTTTTTTGTCGTTTATTGCCTGAAAAAAATACATGGGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTg                                                                                                                                  <  1:592861/143‑1 (MQ=255)                         ccACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                              >  1:802974/1‑151 (MQ=255)                                tttctcttgtttttttttTAATCCATTCTTCTTCCGGCGCAAGCTTTTACAAGAACGGGTA‑AGATAATTTTTTTGTCGTTTATTGCCTTAAAATCTTGCTTGGGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                        <  2:204879/140‑1 (MQ=255)                                tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                       >  1:796863/1‑151 (MQ=255)                                 ttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGcc                                                                                                                      >  1:787541/1‑151 (MQ=255)                                 ttCCGCTCTTTTTTTTTTCATGCATTTTTTTTGCTCCGCAAGCTTTTGCCAAAAGATTTT‑ATATGTTTTTTTTGCCGCTTAATCCATGAAAAACGGGAAGGAGCCCCAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                       <  1:573598/150‑1 (MQ=255)                                    tGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                   >  1:473096/1‑151 (MQ=255)                                     gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGG‑T‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                                 >  2:351688/1‑151 (MQ=255)                                      cTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                                 >  1:344334/1‑151 (MQ=255)                                          ttttttttTCATGCATTCTTCTTGCTCCGCAACCGTTTCCAAAAAGAGTTA‑GGATAATTTTTTTTTCGTTTAATCCCTTAAAAACATGCATGGCCCAAAAAATAAAAAAAAAAAGCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                               <  2:77265/149‑1 (MQ=255)                                          tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                             >  1:62901/1‑151 (MQ=255)                                             ttttttCATGAATTTTTTTTGCGGCGAAGGCTTTTCCAAAAAAAGTTT‑AGATGTTTTTTTTTTCTTTAATTCCCTAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                           <  2:274858/147‑1 (MQ=255)                                              tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                          >  2:950928/1‑150 (MQ=255)                                              tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                          >  1:741445/1‑150 (MQ=255)                                              tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                          >  1:144210/1‑150 (MQ=255)                                                tttCATGCATTTTTTTGCCTGCGAAGGTGTTTCCCA‑AAAAGGTTGAGATGATTTTTTTGTCCATAATTGCCTAAAAAAAATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                          <  2:64920/148‑1 (MQ=255)                                                tttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                       >  2:945409/1‑151 (MQ=255)                                                   cATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                    >  1:540083/1‑151 (MQ=255)                                                      gCATTCTTCTTCCTGCGCAAGCGTTTTCCAGAACAGGTA‑AGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCACAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                  <  1:68645/150‑1 (MQ=255)                                                        attcttctTGCTGCGCAAGCTTTTCCAAAAACAGTTT‑AGATGATTTTTTTGTCGTTAAATGCTTGAAAAACATGCATGACCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                  <  1:933810/146‑1 (MQ=255)                                                        attcttcgTGCTGCCAAGGCGTTGACCAGAGCGGTTA‑AGATGATTTTTTTGCGCCTAAATCGGTATAAAAAATGCAGGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                                 <  1:429741/147‑1 (MQ=255)                                                        aaTCTTTTTGCTGCGCAAGCGTTTCCCAAAACAGTTT‑AGATGTTTTTTTTGCCGTTAAAGGCTTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                      <  1:395788/144‑1 (MQ=255)                                                           cttcttGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                            >  1:518822/1‑151 (MQ=255)                                                                 gctgcGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                       >  1:502101/1‑150 (MQ=255)                                                                     cgcAAGCTTTTCCAAGAACAGGTT‑AATTAATTTTTTTGTCGATAAATGCTTGAAAAACAGCAAGGACCAAAAAAAAAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                   <  1:116810/150‑1 (MQ=255)                                                                     cgcAAGCGTTTCCAAAAAAGGGTT‑AGATGTTTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                       <  1:824577/146‑1 (MQ=255)                                                                          gtttttcCCAAAACCGGTT‑GGATCTTTTTTTGCTCATTTAATCCCTGAAAACCATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                 <  1:790731/144‑1 (MQ=255)                                                                                accacaccgggTA‑AGATGATCTTTTTGCCGTTTAATGCCTAAAAACAATGCCTGACCCACAAAAAAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                                          <  1:185775/139‑1 (MQ=255)                                                                                  cAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                         >  1:794655/1‑147 (MQ=255)                                                                                   aaaacggttt‑AGATATTTTTTTTGTCGTTTAATCCTTAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                      <  2:783991/141‑1 (MQ=255)                                                                                   aGAACAGTTT‑AAATTATTTTTTTGTCGCTAAATGCGTGAAAAACTGGCATGGGCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                         <  2:1063707/146‑1 (MQ=255)                                                                                     aaCAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                                  >  1:58961/1‑151 (MQ=255)                                                                                          gttaagatcttttttttGTGTTTTAATGCCTAAAAACCATCCGTGAGCCACAAAAAAATATAAAAATACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                                 <  2:510499/136‑1 (MQ=255)                                                                                           tt‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                             >  1:73253/1‑150 (MQ=255)                                                                                              agttgatCTTTTTGTCGTTTAATGCTTAAAAAACATGCAGGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                           <  1:271984/147‑1 (MQ=255)                                                                                                 tgttCTTTTTGTCGTTTAATGCCTAAAAAACATGAAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                        <  1:960909/147‑1 (MQ=255)                                                                                                 tgatTTTTTTGTCGTTTAATCCCTGAAAACCAGGCAGGAACCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                          <  1:747947/148‑1 (MQ=255)                                                                                                    tttttttGTCGTTAAATGCCTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                     <  1:1153559/148‑1 (MQ=255)                                                                                                    tCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                    >  1:235826/1‑151 (MQ=255)                                                                                                      tttttGTCTTTAATTCCCTGAAAAACAGGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                    <  1:956065/149‑1 (MQ=255)                                                                                                      tttttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                                  >  1:947554/1‑151 (MQ=255)                                                                                                      tttttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                                  >  2:430162/1‑151 (MQ=255)                                                                                                             cgtttAATGCTTAAAAACCATGAATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                               <  1:978483/144‑1 (MQ=255)                                                                                                              gCTAAATGCCTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                           <  1:33905/150‑1 (MQ=255)                                                                                                               cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                          >  1:451068/1‑150 (MQ=255)                                                                                                               cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                          >  2:689499/1‑150 (MQ=255)                                                                                                                    tGCCTGAAAAATGTGCACGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                     <  2:82258/150‑1 (MQ=255)                                                                                                                    tGCCTAAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                     <  2:1053447/150‑1 (MQ=255)                                                                                                                           aaaccatgaagggcccAAAAAAAAAAAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                                   <  1:1076703/131‑1 (MQ=255)                                                                                                                            aaaaatccttgAGCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                            <  1:462711/142‑1 (MQ=255)                                                                                                                             acaggctgcgggCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                           <  2:526608/140‑1 (MQ=255)                                                                                                                             aaaattcaggatcaacaAAAAAATAGAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                           <  2:585523/137‑1 (MQ=255)                                                                                                                              aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                           >  1:669627/1‑150 (MQ=255)                                                                                                                               catgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGCATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                          >  1:776991/1‑150 (MQ=255)                                                                                                                                   catgCGCCACAAAATAAAAAAAAAACCGCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                     <  1:1105125/151‑1 (MQ=255)                                                                                                                                    atgAGCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                         <  1:544046/146‑1 (MQ=255)                                                                                                                                       acccAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                     <  2:649087/145‑1 (MQ=255)                                                                                                                                        gCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga                 >  2:928602/1‑150 (MQ=255)                                                                                                                                        caaacaAAAAAAAAAAAAAACACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa                <  1:626707/148‑1 (MQ=255)                                                                                                                                         ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat               >  2:724979/1‑151 (MQ=255)                                                                                                                                           aaaaaaaaATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                    <  1:303364/142‑1 (MQ=255)                                                                                                                                           aaaaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata              <  1:978872/148‑1 (MQ=255)                                                                                                                                                ataaaaAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct        <  1:488646/151‑1 (MQ=255)                                                                                                                                                      taAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAggg  >  2:172547/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                       TAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGG  >  NC_000913/29348‑29646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑