Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,326,450 | 0 | T | G | 38.2% | 102.9 / 13.4 | 56 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
Reads supporting (aligned to +/- strand): ref base T (3/31); new base G (21/0); total (25/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.40e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAAC > NC_000913/2326304‑2326600 | gcgcGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATgg < 2:81872/151‑1 (MQ=255) gcgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAAGCGCAGAGCATTGTGGCGGGGGGGATgg > 2:696778/1‑149 (MQ=255) gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAA‑TGAGtttt > 1:1173416/1‑150 (MQ=255) aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGc < 2:1068483/150‑1 (MQ=255) aGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCt < 2:1035292/151‑1 (MQ=255) gCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCta < 2:23852/151‑1 (MQ=255) cGGGCTGGCAGAAACGGTGTGCGGATTTACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATA‑TGAGGTTAGccccc > 2:718818/1‑148 (MQ=255) gggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCtact < 1:1183531/151‑1 (MQ=255) cTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAA‑TGAGTTTAGCCCCCtactt > 2:853887/1‑149 (MQ=255) cAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAA‑TGAGAGTTGCCCCCtaccttcac > 2:487342/1‑143 (MQ=255) aaCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCa < 1:954962/151‑1 (MQ=255) ggTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCaaaa < 2:233902/151‑1 (MQ=255) gtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa < 2:544257/151‑1 (MQ=255) gtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTGGGAAATA‑TAAGGTTAGCCCCCCACCTACTCGGTGCaaaa > 1:1094633/1‑148 (MQ=255) cGGATTCACGGTCAATAAAGTATGTGGTTCGGGACTTAAAAGTGTGGCGCTTGCCGCCCAGGACATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGCGGGGAATTTAAATA‑TGAGCTTAGCCCCCCCCTTACTCGATTCACAATCACGc > 1:719165/1‑151 (MQ=255) ttCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAA‑TGGGTGTTGCCCCCTACTTTATCGATGCCAAAGCACGctct > 1:572777/1‑150 (MQ=255) tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg < 1:1097491/150‑1 (MQ=255) tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAA‑TGAGTTTAGCCCCCCACTTACTCGAAGCAAAAGCaacgtc > 1:991907/1‑143 (MQ=255) tAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATC‑GTCtt > 2:677747/1‑151 (MQ=255) aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCtt < 2:603112/150‑1 (MQ=255) aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCtt < 2:1229482/150‑1 (MQ=255) aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCtt < 1:663513/150‑1 (MQ=255) aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGga < 2:730082/151‑1 (MQ=255) aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGAc < 2:571940/151‑1 (MQ=255) aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATA‑TGGGTTTAGCCCCCTACCTACTCGATGCAAAAACACGCCCTGGTTATC‑GTCTTGgaga > 2:618398/1‑150 (MQ=255) tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAATA‑TGAGTTTTGCCCCCCACTTACTCCATGGAAAAACACGCTCTGGTTATT‑GTCTTGGGGACg > 1:35406/1‑151 (MQ=255) gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACgg < 1:776048/151‑1 (MQ=255) gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACa < 2:1172057/151‑1 (MQ=255) tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAg < 1:235294/150‑1 (MQ=255) cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAA‑TGAGTTTAACCCCCCACTTAATCGATGCAAAAACAAGCTCTGGTTATT‑GTCTTGGGGGCGGAAAgg > 2:707263/1‑150 (MQ=255) gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGCCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGtt < 2:927724/151‑1 (MQ=255) aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAA‑TGAGTTTAGCCCCCTACTTACTCGATGCCAAAACACGCTCTGGGTATC‑GGCTTTGGGACGGACAGGGTTATTACg > 2:850968/1‑149 (MQ=255) aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAA‑TGAGTTTTGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGTTTTT‑GTCTTGGAGACGGACAGGGTTATTACg > 1:18238/1‑149 (MQ=255) tgtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATA‑TGGGGTTTGCCCCCCCCCTTCTCGCTTCCAAAAGAAGCTCTGGTTATCGGCCTTGGGGA‑GGACAAGGTTTTGACGCaa > 1:477915/1‑149 (MQ=255) cgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGc < 1:620243/151‑1 (MQ=255) ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAt < 1:50992/150‑1 (MQ=255) ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATTGGGTTAAGCCCCC‑ACTTTCTTCGTGGAAAAACACGCTCTGGTTATC‑GGCTTGGGGACGGACAGGGTTATGACGGCATTCTGCGCGATGGGCTGGtgtggg > 2:84028/1‑148 (MQ=255) ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATA‑TGAGGTTTGGCCCCCACCTACTCGATGCAAAAAGAAGCTCTTGGTATC‑GTCTTGGGGACGGAAAGGGTTATGAAGTAAACCTGCGCGATgg > 1:1201096/1‑139 (MQ=255) tCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTGGGAAATA‑TGTGTTTAGCCCCCCACTTACTCGGTTCAAAAGCCCGCTCTGGTTATT‑GTCTTTGGGAGGGACAGGTTTTTTACGTAAACCTCCGCGATGGCCTGGTGTgcggc > 2:555380/1‑149 (MQ=255) caggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCa < 1:1077589/151‑1 (MQ=255) aggTCAGGCGCAGAGCATTGTGGCGGGGGGGGTTGAAAAAA‑TGTGGTTAGCCCCCTACTTATTCGATGCCAAAAGACGCTCTGGTTTAC‑GGTTTTGAGAGGGACAGGCTTATGACGTAATTCCGCGCGGTGGCCTGCTGGGCGCcccc > 1:404232/1‑148 (MQ=255) cAGGCGCAGAGCATTGTGGCGGCGGGGATGGGAAAAA‑TGAGTTTAGCCCCCCACTTAATCGATGCCAAAACACGCTCTGGGTATC‑GTCTTGGAGAAGGACAGGGTTATGACGGAATTCTGCGCGATGGCCTGATGTGCGCCACCCATGGtt > 2:855701/1‑151 (MQ=255) cgcAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCa < 2:30156/151‑1 (MQ=255) gcAGAGCATTGTGGCGGGGGGCATGGAAAATA‑TGAGTTTAGTCCCCTACTTACTCGATGCAAAAGCACCCGCTTGTTATC‑GTCTTGGAGACGGGCAGGATTATGAAGGAATCCTGCGCCATGGCCCGATGGGCGCCACCCCTGGttctga > 2:228872/1‑146 (MQ=255) agCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATgg < 1:852886/151‑1 (MQ=255) agCATTGTGGCGGGGAGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATAAAAAAGCACGCTCAGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTCATCCTGCGCGATGGCGTGATGTGAGCCACCCATGGGTATCATATg < 1:726841/150‑1 (MQ=255) tgtgGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAt < 2:521459/150‑1 (MQ=255) gtgGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGAtt < 1:604982/150‑1 (MQ=255) ggCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAcc < 2:214868/151‑1 (MQ=255) cGGGGGGTATGGAAAATA‑TGAGTTTAGCCCTCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgc < 2:695534/151‑1 (MQ=255) ggggggTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgcc < 2:648376/151‑1 (MQ=255) ggggggTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgcc > 1:1074265/1‑151 (MQ=255) ggggggTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgcc > 2:909403/1‑151 (MQ=255) gggggTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTAccgccg > 2:77945/1‑151 (MQ=255) ggTATGGAAAATA‑TGAGTTTAGCCCCCTAC‑TACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaaaa < 2:1108272/151‑1 (MQ=255) gTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaaaa < 2:840589/151‑1 (MQ=255) tATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAAc > 2:35205/1‑151 (MQ=255) | GCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATA‑TGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC‑GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAAC > NC_000913/2326304‑2326600 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |