Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,350	0	C	T	38.2%	70.6 / 26.0	55	S64F (TCT→TTT)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (8/26);  new base T (21/0);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 5.18e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATT  >  NC_000913/4604201‑4604500                                                                                                                                                      |                                                                                                                                                       aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                        <  2:355257/150‑1 (MQ=255)       aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg                                                                                                                                                 <  1:329561/151‑1 (MQ=255)              aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                           <  1:562164/150‑1 (MQ=255)                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGa                                                                                                                                         <  1:419926/150‑1 (MQ=255)                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                                        <  2:768083/151‑1 (MQ=255)                 agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGGGAAggg                                                                                                                                          >  2:822204/1‑146 (MQ=255)                    agtaTCGGTCTAGAGAGCTTATTCAGAAAGTTTGCGAGTAACTCTTATATGCTCCATCCCTATTCCAGTCAGGAGTTATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAGTTTTTCTTTTTCTGCCATGAGCAGTTGgcgc                                                                                                                                     <  1:1168080/150‑1 (MQ=255)                    agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAgcg                                                                                                                                      >  2:154204/1‑149 (MQ=255)                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGGGAAGTGAGCGCGGAGAggg                                                                                                                             >  2:327605/1‑149 (MQ=255)                              ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGggggggggt                                                                                                                          >  1:907772/1‑148 (MQ=255)                               tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGCAGAGGGGGGtt                                                                                                                          >  1:1171617/1‑150 (MQ=255)                               tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAAGGAGGGCAGGGAGGGGtt                                                                                                                          >  1:21519/1‑150 (MQ=255)                                gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                         <  2:782913/150‑1 (MQ=255)                                gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                        <  2:393146/151‑1 (MQ=255)                                 agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGTGAGCGCAGAGAGGGGTTAt                                                                                                                        >  2:829181/1‑150 (MQ=255)                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                                     <  1:91296/151‑1 (MQ=255)                                          tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctga                                                                                                               <  1:929830/150‑1 (MQ=255)                                          tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctga                                                                                                               <  1:748629/150‑1 (MQ=255)                                           cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGCCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCATTGGAAGGGAGCGGCGGGAGGGGTTTTTTTGCctggat                                                                                                             >  1:937981/1‑148 (MQ=255)                                            aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTCTTTTTTTTCCCTATGAAGTGAGCGCAGGGGGGGAGTAttttttctggat                                                                                                             >  1:1151742/1‑142 (MQ=255)                                            aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGGGAAATGAGCGCGGAGGGGGGTTTTATTTCctga                                                                                                               >  2:801292/1‑148 (MQ=255)                                             gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactga                                                                                                           <  2:738737/151‑1 (MQ=255)                                               aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGGGCGCCGAGAGGGGGTATATTTCCTGACTGCAc                                                                                                         >  2:613829/1‑151 (MQ=255)                                                aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGTGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTGGCTGaa                                                                                                          >  2:463952/1‑149 (MQ=255)                                                 gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGAAATTTTTTCTTTTTCTGCCATGAGTAGTGAGGGCAGGGAGGGGTTATCTTGGCTTACTGTAc                                                                                                         >  2:531501/1‑149 (MQ=255)                                                   ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAACTgg                                                                                                      >  2:118494/1‑150 (MQ=255)                                                     gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCg                                                                                                    <  1:395164/150‑1 (MQ=255)                                                            aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                                                                                            <  1:373088/151‑1 (MQ=255)                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGGGGGGGGTTTTTTTTCCTGACTGAACTGGGGATTAAGttt                                                                                           >  1:181404/1‑149 (MQ=255)                                                                         ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTTCCTGTGAAGGGAGCGCGGGGAGGGGGTATCTTGCCTGGCTTAACTGGCGCTTAAGTTTCCGCGtgaccg                                                                                >  1:581999/1‑145 (MQ=255)                                                                         ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                                                 <  1:246088/149‑1 (MQ=255)                                                                         ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                                                                               <  1:826787/151‑1 (MQ=255)                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                          <  2:956051/151‑1 (MQ=255)                                                                                tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGGGGAGCGAGCGCAGAGAGGGGTTTTCTTTGCTGTCTTGACTGGCGATTTAGATTCCGCGTACCCCGCGttt                                                                          >  1:110080/1‑149 (MQ=255)                                                                                 ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                        <  1:159154/150‑1 (MQ=255)                                                                                   aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCGGAGAGGGGTTATTTTGCCTGACTGAACTGGGGATTTAGTTTCCGCGTAACCGGCGTTTTGt                                                                       >  1:357343/1‑149 (MQ=255)                                                                                     cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAAGGAGGGCCGGGGGGGGTTTTTTTTGCTTACTGAAATGGGGGTTAAGTTTTCGCGGACCCCGCGGTTTGTtttt                                                                   >  1:669957/1‑151 (MQ=255)                                                                                         cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAGCGCAGGGAGGGGTTATTTTGCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCGGCGGTTAGTTATTGCgg                                                               >  1:233979/1‑151 (MQ=255)                                                                                              gTCATTTCAGGATGCCATGTCGCGGATCTCGATTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGTCTGACTGAACTGGCGATTAAGTTTCCGCGTCCCCGGCGTTTAGTTATTGCGgatgat                                                          <  1:212072/151‑1 (MQ=255)                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCCCGGACCCCGCGGTTTGTTTTTGCggg                                                              >  2:961187/1‑144 (MQ=255)                                                                                                 aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                                       <  2:1252822/151‑1 (MQ=255)                                                                                                   ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGata                                                      <  2:576860/150‑1 (MQ=255)                                                                                                     cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                                    <  2:581254/150‑1 (MQ=255)                                                                                                          tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGGAGGGAGCGGAGGGAGGGGTTTTCTTGTCTTACTGAACTGGCGGTTAAGGTTTCGCGGTCCCCGCCTTTTGTTATTGCGGGTTTTGAGATTGaa                                                 >  2:837373/1‑148 (MQ=255)                                                                                                           gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTc                                             <  2:1247142/151‑1 (MQ=255)                                                                                                            ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                            <  2:827495/151‑1 (MQ=255)                                                                                                              aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTTTCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTAGTTTTTGCGGGTGATGATATTGAAGCTCgg                                           >  2:749321/1‑150 (MQ=255)                                                                                                                  cgcgGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCGGGGGGGGGTTATTTTTTCTTGCTGAACTTGCGGTTAAGTTTTCGCGGACCCCGCGGTTAGTTTTTTCGGGTGGTGATATTTAAGCTCGGCTggt                                      >  1:1207985/1‑149 (MQ=255)                                                                                                                       aTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCCTGAGAAGTGAGGGCAGAGGGGGATTTTCTTTCCTGAATGAAATGGCGGTTAAGTTTCCGCGTACCCGGCGGTTAGTTATTGCGGATGATGATTTTTGAGCTCCGCTGATTgg                                   >  2:862045/1‑149 (MQ=255)                                                                                                                        tctcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGGGGAGGGAGGGGCGAGAGGGGTTTTATTTTCTGACTGAAATGGCGGTTAAGTTTTCGCGGACCCGGGGTTTTGGTATTTCGGGTGATGGTATTGTAGCTCGGGCggttggtt                                 >  2:558118/1‑150 (MQ=255)                                                                                                                               tGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc                          <  2:89186/150‑1 (MQ=255)                                                                                                                                    cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc                    >  2:833797/1‑151 (MQ=255)                                                                                                                                    cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc                    <  2:78018/151‑1 (MQ=255)                                                                                                                                      gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc                  >  2:1034941/1‑151 (MQ=255)                                                                                                                                      gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc                  <  2:455443/151‑1 (MQ=255)                                                                                                                                         aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg                >  1:767475/1‑150 (MQ=255)                                                                                                                                           tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt              <  1:840041/150‑1 (MQ=255)                                                                                                                                           tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg             >  2:996467/1‑151 (MQ=255)                                                                                                                                             tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg            >  1:689398/1‑150 (MQ=255)                                                                                                                                                  ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGgtg      >  2:827768/1‑151 (MQ=255)                                                                                                                                                   tttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGgtg      <  1:184141/150‑1 (MQ=255)                                                                                                                                                      ctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTAtt  >  1:262647/1‑151 (MQ=255)                                                                                                                                                      ctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATT‑AAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTAt   <  1:154200/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       AGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATT  >  NC_000913/4604201‑4604500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑