Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 358,399 T→G T98P (ACC→CCC)  cynR ← transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913358,3990TG98.4% 224.2 / NA 62T98P (ACC→CCC) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (32/29);  minor base C (1/0);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTA  >  NC_000913/358252‑358541
                                                                                                                                                   |                                                                                                                                              
aGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGa                                                                                                                                              >  1:647556/1‑150 (MQ=255)
    aaTCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGc                                                                                                                                          <  2:86510/150‑1 (MQ=255)
    aaTCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCg                                                                                                                                         <  2:592166/151‑1 (MQ=255)
     aTCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCg                                                                                                                                         >  1:570246/1‑150 (MQ=255)
          aaCGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGc                                                                                                                                   <  1:1197324/151‑1 (MQ=255)
             gTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGc                                                                                                                                >  1:1129026/1‑151 (MQ=255)
             gTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGc                                                                                                                                <  2:418700/151‑1 (MQ=255)
             gTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGc                                                                                                                                <  1:768600/151‑1 (MQ=255)
              tCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGc                                                                                                                                >  1:760322/1‑150 (MQ=255)
                cAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGAt                                                                                                                             >  1:863810/1‑151 (MQ=255)
                 aaCTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGAt                                                                                                                             >  2:1098951/1‑150 (MQ=255)
                  aCTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATcc                                                                                                                           >  1:961208/1‑151 (MQ=255)
                   cTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAACGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGCGGGGGGGACGGCGATACGCAGCGATcc                                                                                                                           >  2:1043582/1‑150 (MQ=255)
                       tcgCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTcgcg                                                                                                                      >  2:811426/1‑151 (MQ=255)
                        cgcgGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTcgcg                                                                                                                      <  2:941761/150‑1 (MQ=255)
                         gcgGCAAAGTATATCCTCGCTTTTCTCCAACGACATTTCCTGTAGCCGGAGCGTGATGCTGGGATAGCGCGCATAGTTATCCGCCTTTATGAGGCCGATCAAGTAGCTCGTAACGGAGGCGGCGACGGCTCTACACCGCGATCCTCGCGt                                                                                                                     >  1:945167/1‑150 (MQ=255)
                         gcgGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTc                                                                                                                         <  2:49531/146‑1 (MQ=255)
                              aaaGCATATCCTGGATTTTCGCCTGCGACATTTCCTGTCGCTGGGGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATc                                                                                                               <  1:19506/151‑1 (MQ=255)
                                   atatCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCaa                                                                                                          <  2:726288/151‑1 (MQ=255)
                                   atatCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCa                                                                                                           >  1:402871/1‑150 (MQ=255)
                                   atatCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAACCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCa                                                                                                           <  1:377554/150‑1 (MQ=255)
                                    tatCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAc                                                                                                         >  1:96307/1‑151 (MQ=255)
                                     acccTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAc                                                                                                         <  2:66899/148‑1 (MQ=255)
                                       ccTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAcatc                                                                                                      >  2:307481/1‑151 (MQ=255)
                                           gATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGa                                                                                                  <  1:207394/151‑1 (MQ=255)
                                                tctcCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCg                                                                                              <  2:676914/150‑1 (MQ=255)
                                                 ctcCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGcc                                                                                            >  1:708610/1‑151 (MQ=255)
                                                  tcCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGccc                                                                                           >  2:462522/1‑151 (MQ=255)
                                                   ccTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCg                                                                                          <  1:972113/151‑1 (MQ=255)
                                                        gACATTTCCTGTAGCTGGAGCGTGAGGCGGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCACCCGttt                                                                                       <  1:396443/149‑1 (MQ=255)
                                                         aCATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTAcc                                                                                    >  2:838478/1‑151 (MQ=255)
                                                           aTTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCg                                                                                  >  2:663486/1‑151 (MQ=255)
                                                            tttCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGAAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGACAGATCGGCAACATCATGAATCGCCCGTTTACCCGc                                                                                 >  2:61762/1‑151 (MQ=255)
                                                              tCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGcc                                                                                <  2:550144/150‑1 (MQ=255)
                                                               ccTGTAGCTGGAGCGTGATGCTGGGATAGGGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGcc                                                                                <  2:1175711/149‑1 (MQ=255)
                                                                    aGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAACCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGccccc                                                                             <  1:832430/147‑1 (MQ=255)
                                                                              tGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGc                                                               >  2:190157/1‑151 (MQ=255)
                                                                               gaagCTGGGAAGGCGCCCGTAAAAATCCGCCATTAAAGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGTCGATACGCAGCGATCCTCGCGTCAGATCGGCAACCTCATGAATCGCCTGTTTACCCGCACCCAGTTCCTGTAAc                                                                 <  1:152663/145‑1 (MQ=255)
                                                                                aTGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGcc                                                              <  2:62844/150‑1 (MQ=255)
                                                                                  gCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCgg                                                           >  2:252443/1‑151 (MQ=255)
                                                                                  gCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACACGCCCCCAGTTCCTGTAACGCCCgg                                                           >  1:162988/1‑151 (MQ=255)
                                                                                     gggATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGc                                                          <  2:899677/149‑1 (MQ=255)
                                                                                     gggATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTg                                                        <  2:607946/151‑1 (MQ=255)
                                                                                      ggATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTgg                                                       >  1:50894/1‑151 (MQ=255)
                                                                                                cATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTc                                              <  2:127476/150‑1 (MQ=255)
                                                                                                 aTAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGc                                            >  2:23841/1‑151 (MQ=255)
                                                                                                 aTAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGc                                            >  2:74183/1‑151 (MQ=255)
                                                                                                         ccGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTtc                                    >  1:733168/1‑151 (MQ=255)
                                                                                                             cATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCt                                >  1:644167/1‑151 (MQ=255)
                                                                                                               ttAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGc                              >  1:1120213/1‑151 (MQ=255)
                                                                                                                  aGGGGCCGATAAAGTAGCTCGTAAAGGTGGGTGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCAt                            <  2:639756/150‑1 (MQ=255)
                                                                                                                   ggggCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATc                           >  1:485506/1‑150 (MQ=255)
                                                                                                                   ggggCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCa                          <  2:344207/151‑1 (MQ=255)
                                                                                                                    gggCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATc                           >  1:613693/1‑149 (MQ=255)
                                                                                                                     ggCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAg                         >  1:19704/1‑150 (MQ=255)
                                                                                                                       ccGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTga                      <  1:30059/151‑1 (MQ=255)
                                                                                                                       ccGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTga                      <  1:1181139/151‑1 (MQ=255)
                                                                                                                        cGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTg                       >  1:401911/1‑149 (MQ=255)
                                                                                                                         gATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTg                       >  2:841541/1‑148 (MQ=255)
                                                                                                                                   cTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTc          >  2:921608/1‑151 (MQ=255)
                                                                                                                                          aGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTATTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCt   <  1:190153/151‑1 (MQ=255)
                                                                                                                                           ggTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTa  <  2:1241335/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTA  >  NC_000913/358252‑358541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑