Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG40.7% 125.8 / 31.8 83V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (4/44);  new base G (33/0);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 1.23e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAC  >  NC_000913/599429‑599721
                                                                                                                                                   |                                                                                                                                                     
cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGggggggggggg                                                                                                                                                    >  1:371781/1‑150 (MQ=255)
      acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGgggggggggggg                                                                                                                                                   >  2:79629/1‑144 (MQ=255)
        gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGCGGggggtggtgc                                                                                                                                               >  1:118207/1‑144 (MQ=255)
              aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGACCTGCGGTcc                                                                                                                                      >  2:225861/1‑150 (MQ=255)
              aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGTGATcc                                                                                                                                      >  1:1233390/1‑150 (MQ=255)
                 cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCa                                                                                                                               <  2:1331539/151‑1 (MQ=255)
                   caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaa                                                                                                                             <  1:948865/151‑1 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTACTGCGGGACGGCaa                                                                                                                              >  1:1264730/1‑149 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCcaa                                                                                                                             >  2:239925/1‑150 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCcaa                                                                                                                             >  1:1233960/1‑150 (MQ=255)
                               aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGGTTCTGCGAGCCGGGCAAAAACCCCga                                                                                                                    >  1:1069204/1‑148 (MQ=255)
                                gCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGaa                                                                                                                 <  1:93301/150‑1 (MQ=255)
                                 ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGt                                                                                                               <  1:1212081/151‑1 (MQ=255)
                                 ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGt                                                                                                               <  2:696426/151‑1 (MQ=255)
                                   aGTGAAAATGGCGTGCCCGTTTATCTGCGCTATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGa                                                                                                             <  1:730171/151‑1 (MQ=255)
                                   aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGa                                                                                                             <  2:310842/151‑1 (MQ=255)
                                    gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGCGGTCCGGCAAAAACGCCCGGGAAGTg                                                                                                              >  1:940502/1‑149 (MQ=255)
                                      gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATc                                                                                                           <  1:708603/150‑1 (MQ=255)
                                       aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgc                                                                                                         <  1:878500/151‑1 (MQ=255)
                                        aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGAGGGGGTCCCGCGATCCAGGAACAAAGTCCGGGAAGCGAt                                                                                                            >  1:142632/1‑147 (MQ=255)
                                        aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgcc                                                                                                        <  2:318696/151‑1 (MQ=255)
                                        aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgcc                                                                                                        <  1:227926/151‑1 (MQ=255)
                                            ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                     <  2:418614/150‑1 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGATCCTTCGCTACCGCGAAAACGCCCGAGAAGGGTTGGccgccg                                                                                                    >  1:320068/1‑148 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa                                                                                                 <  1:1157921/151‑1 (MQ=255)
                                                  cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGGAGGGATCGCCGCCGTGAAgg                                                                                              >  1:805848/1‑151 (MQ=255)
                                                  cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCCGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAgg                                                                                              >  1:253348/1‑151 (MQ=255)
                                                       ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaa                                                                                          <  1:480642/150‑1 (MQ=255)
                                                       ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaa                                                                                          <  1:728638/150‑1 (MQ=255)
                                                       ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaa                                                                                          <  2:105479/150‑1 (MQ=255)
                                                        tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCTCGGTCCGGCAAAAAAGCCCCAGAAGTGAGcggccccccgcaggac                                                                                            >  2:196453/1‑133 (MQ=255)
                                                         aTCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAAc                                                                                        <  2:201666/150‑1 (MQ=255)
                                                          tCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAAAGCCCCAGAAGGGATCGCCGCCGGGGAGGACaaa                                                                                         >  2:861163/1‑148 (MQ=255)
                                                          tCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGCGGGGGGGGTGATCCTCGGTAACGGCGAAAAACCCCGAAAAGGGATAGCCCCCGCGGAGGCCAAACTg                                                                                      >  1:102003/1‑151 (MQ=255)
                                                           cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGCGGGGGGGGGGGCCCTCCGACCCAGCAAAAACGCCCGCGGAGTGGTGGCCGCCGGGAAAGGCaaaactg                                                                                     >  2:1262743/1‑147 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTgg                                                                                     <  1:1310481/149‑1 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa                                                                                   <  1:1203721/151‑1 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa                                                                                   <  2:857653/151‑1 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGGGTGACGGCAAAAAACCCCCAGGAGTGGTTGCCCCCCTGAAggggaa                                                                                          >  2:1150473/1‑140 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAGCCCGCCAAAAAGCCCGAGAAGGGAACGCCCCCGCGCAGGACAAAATgg                                                                                     >  1:1622/1‑149 (MQ=255)
                                                              cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGTAGGGTGGTTATCCTCCAAGCCGGCCAAAACATCCGAGTGATGATTGCCGGCGGGAAGGACAAAccgata                                                                                   >  1:435664/1‑145 (MQ=255)
                                                               gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAc                                                                                 <  1:1093024/151‑1 (MQ=255)
                                                                 gATGTTGCGAAGGTCCGGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc                                                                               <  1:578094/151‑1 (MQ=255)
                                                                  aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCt                                                                              <  2:732590/151‑1 (MQ=255)
                                                                   tGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTCCGGTCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGGAGGACAAAATGGAAACGCt                                                                              >  1:825104/1‑150 (MQ=255)
                                                                      tGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGGTCCTGCGGTCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGGCAAACTGGAAACACTGaa                                                                           >  1:1139324/1‑150 (MQ=255)
                                                                      tGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaa                                                                           <  2:1241565/150‑1 (MQ=255)
                                                                         gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAGCCTGCGGGCCCGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGGAGGACAAAACGGAAACGCTGaaa                                                                          >  1:572399/1‑148 (MQ=255)
                                                                         gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGAATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAag                                                                        <  1:267849/150‑1 (MQ=255)
                                                                                 aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATGCTTAGAGCTGCGCAAAAAGCCCAGGCAGGGCTGGGCGGCGTGAAGGGCAAACAGGAAAcagcgagaaggatga                                                                   >  2:659272/1‑133 (MQ=255)
                                                                                   aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGCGATCCCGCAAAAAAACCCCGGGAAGGGGGGCCGCAGCGGAAGACAAACTGGAAAACCTGgaaatgtggcggccgg                                                             >  2:961655/1‑139 (MQ=255)
                                                                                     tGGCCCGGAGATGCGCCGGGGCATTGCCGAACTGAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGGTCCGGCAAAAAACCCCGAGAAGGGGTCGCCGCCGGGAAGGGAAAACAGGGAACGCTGAAAAGGAGGCTGCCgg                                                             >  2:747274/1‑149 (MQ=255)
                                                                                      ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCCGGGGGGGGGTCCCGCGGGCCCGGCAAAACGCCCCCGGAGTGATCGCCGCCCTGCAGGGCAAACTGGGAACGGTGgaaaagaacgtggccg                                                             >  2:355256/1‑136 (MQ=255)
                                                                                       gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAgg                                                         <  2:235317/151‑1 (MQ=255)
                                                                                        cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGATCCCGCAAAAAAGCCCGGGAAGGGAACGCCGCCGGGGAGGACAAAACGGAAAAGCTGAAAAGGAGGCTGCCGGAAgg                                                         >  2:1275726/1‑150 (MQ=255)
                                                                                         ccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGATCCGGCCAAAACGCCCGGGAAGGGATCGCCGCCGGGGAGGACAAACTGGAAACGCTGAAAAGGAGTCAGCCGGGAgg                                                         >  2:1185914/1‑149 (MQ=255)
                                                                                           ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGAGGGGGTCCTGCCCTCCCGGAAAAACGCCCGAGAAGTGGTTGCCGCCGTGAAGGACAAAATGGAAACGCTGAAAAAGAGTCTGGCGGGAGGCg                                                       >  1:533818/1‑149 (MQ=255)
                                                                                            gagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                    <  1:879961/151‑1 (MQ=255)
                                                                                              gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGga                                                   <  1:403296/150‑1 (MQ=255)
                                                                                                tGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGGAAAAAAGCCCCAGAAGTGATCGCCCCCGTGAAGGACAAACCGGAAAAGCTGAAAAGAAGTATGCCGGAAGGCgggggg                                                  >  1:228752/1‑147 (MQ=255)
                                                                                                 gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGGCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATa                                               <  2:1248427/151‑1 (MQ=255)
                                                                                                  cgcCGGGGCATGGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATa                                               <  2:871808/150‑1 (MQ=255)
                                                                                                      ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATTGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                           <  2:111896/150‑1 (MQ=255)
                                                                                                       gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                         <  1:29583/151‑1 (MQ=255)
                                                                                                       gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGTTCCTGGAAAAACGCCCGTGAAGAGATAGCCCCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGGTTGCCGGGAAGCGGGGGGAAAGtt                                            >  1:741385/1‑148 (MQ=255)
                                                                                                           aTTGCCGAACTAAACGGGGAAGGCGAAGTGGCGGGCGGGGGGGAGGGCCTCGCATTCGGGCAAAAACGCCCAGGAGGGAACGCCGGCCGGGAGGGCCAACAGGGAAAACGGAAAAAGAGGCAGGCGGGAGGCGGGGAGAAAGGTacaaa                                       >  2:40173/1‑148 (MQ=255)
                                                                                                            ttGCCGAACTAAGCGGCGAAGGCGATGTGGCGGGCGGGTTGGTGATCCTGCGATCCGGCAAAATCGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACAt                                     <  2:792635/150‑1 (MQ=255)
                                                                                                              gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGAGAGCCTGCGCTCCCGCAAAAAAGCCCCAGGAGGGATCGCCGCCGGGTATGGCAAAACGGAAACGCCGAAAAGGAGACTGCCGGAAAGCGGGGGGGAAGTTACAACATAc                                   >  1:883307/1‑150 (MQ=255)
                                                                                                                  aaCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCg                              <  2:580185/151‑1 (MQ=255)
                                                                                                                   aCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGc                             <  1:225867/151‑1 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagc                          <  1:251558/151‑1 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGca                            <  2:563139/149‑1 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAAAGCCCGAGAAGAGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGGGTGGAGATAGTTAAAACATACGATCGcag                           >  1:589362/1‑150 (MQ=255)
                                                                                                                      aaaCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGTCCGGCAAAAACGCCCGAGGAGTGGTCGCCGCCGTGAAGGACAAAATGGAAACGCTGAAAAGTAGTCTGCCGGAAGGGGGGGGGATAGTTACAACATAAGATCGc                             >  1:781821/1‑148 (MQ=255)
                                                                                                                        aCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcca                        <  1:1184968/151‑1 (MQ=255)
                                                                                                                          ggcgaaggcgaaGTGGCGGGCGGGGTGGTGATCCTGCGAGCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagccag                       <  2:537266/150‑1 (MQ=255)
                                                                                                                             gaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTc                    <  1:60753/150‑1 (MQ=255)
                                                                                                                             gaaggcgaagTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGGGGGGATAGTTACAACATACGAACGcagccagccc                    >  1:281049/1‑148 (MQ=255)
                                                                                                                             gaaggcgaagTGGCGGGCGGGGGGGGGAGCCCTCCGCCCCGCAAAAAAACCCGCGGAGTGGTCGCCGGCGGGAAGGACAAACAGGGAACGCCGGAAAGGAGTATGCCGGGAAGCGGGGAGATAAGTAAAAAATAcagaatcagcccactc                    >  2:1246065/1‑135 (MQ=255)
                                                                                                                                    aagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcg            <  2:349279/151‑1 (MQ=255)
                                                                                                                                    aagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcg            <  1:676106/151‑1 (MQ=255)
                                                                                                                                         gcgggcggGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGcc        >  1:1326007/1‑150 (MQ=255)
                                                                                                                                           gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc     >  2:382029/1‑151 (MQ=255)
                                                                                                                                           gggcgggGTGGCGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc     >  1:1301882/1‑151 (MQ=255)
                                                                                                                                            ggcgggGTGTTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg    <  1:1186672/151‑1 (MQ=255)
                                                                                                                                              cgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc  <  1:634339/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAC  >  NC_000913/599429‑599721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: