Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,228,275	0	T	C	41.6%	93.7 / 11.8	78	pseudogene (335/1287 nt)	mdtQ	pseudogene; putative channel/filament proteins
Reads supporting (aligned to +/- strand):  ref base T (32/13);  new base C (4/28);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 2.54e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCC  >  NC_000913/2228126‑2228419                                                                                                                                                            |                                                                                                                                                   gCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTc                                                                                                                                                   <  1:834818/151‑1 (MQ=255) gCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTACTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCt                                                                                                                                                    <  1:70083/150‑1 (MQ=255)    ctTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTcc                                                                                                                                                  <  2:757845/149‑1 (MQ=255)    ctTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCGCGGT‑TCCCCC‑CCCCCGC‑TTTTA‑CC‑CTACCCCAGCGGGGCGTAAACTcccc                                                                                                                                                >  2:1285560/1‑149 (MQ=255)      ttttCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTTCTCGCGTT‑TCGCCG‑CCCGTGC‑TTTAA‑CC‑CGACCCCGGCGGGCAGGAACCTCCGccc                                                                                                                                              >  1:262925/1‑151 (MQ=255)       ttgcTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTCCCCAGGCGGGCAGTAACCTCCGccc                                                                                                                                              <  1:215116/147‑1 (MQ=255)          cTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCCGG‑TCTGCTCGCGCT‑CCGCCA‑CCCCAGT‑CTTTA‑CC‑GGATACCGGAGGGCAGTAAACTCCGCCCGCtt                                                                                                                                          >  1:430825/1‑151 (MQ=255)            aTTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTGCAGGCGGGCTACGCTGCCAGCCACCAATTGGCGGG‑TGTGACTGCGCG‑TCGCCC‑GCCGCGG‑TTTAA‑CC‑ATACCCCAGGGGGCCGTAATCTcctgccggctc                                                                                                                                         >  2:902660/1‑141 (MQ=255)              ttGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCtt                                                                                                                                       <  1:574305/150‑1 (MQ=255)              ttGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCGCGGT‑CCGCCC‑CCCCCGC‑TTTTA‑AC‑CCACCCCCGCGGGCAGGAACCTCCCCCCGGTTCttt                                                                                                                                      >  2:636540/1‑151 (MQ=255)                   gcAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCTGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCa                                                                                                                                 <  1:557682/151‑1 (MQ=255)                    cAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCC‑CCCCTGC‑TTTAA‑CC‑CTACCCCGGCGGGCAGTAACCTCCCCCCGATTTTTTtccc                                                                                                                                  >  1:605835/1‑149 (MQ=255)                     aaGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑a                                                                                                                              <  2:1255180/151‑1 (MQ=255)                         ccGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCCCCG‑CCCCTGC‑CTTTA‑CC‑GTACCCCGGCGGGCAGTAACCTCCCCCCGATTCTTTTCCCCG‑ata                                                                                                                            >  1:46068/1‑149 (MQ=255)                             gtTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTTA‑CC‑CTACCCAGGCGGGGAGGAACCTCCCCCCGATTCTTTtccc                                                                                                                                  >  1:1186919/1‑140 (MQ=255)                                 aaCGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTTCTCCCGTT‑TCCCCC‑CCCCCGC‑TTTTA‑AC‑CTACCCCCGGGGGGAGGAAACCCCCCCCGATTTTTTTCCCCC‑ATATC‑GAGGTGcc                                                                                                                 >  2:507569/1‑151 (MQ=255)                                    gccgccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCCGCAATTGGCGGG‑GTTTCTCGCCGT‑CCCCCG‑CCCCCGC‑CTTTA‑CC‑CTACCCAGGCGGGGAGTAACCCCCCCCCCCTTCTTTTCCCCG‑AAATC‑CAGGTggc                                                                                                                 >  2:318923/1‑146 (MQ=255)                                     ccgccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGcc                                                                                                             <  2:1262811/151‑1 (MQ=255)                                       gccTGGGTTTGCCACTCCCAGTACAGGCGGGCTCCGCTGCCAGCCAACAAATGGCGGG‑GTTGCTCGCGGT‑TCGCCGCCCCGTCG‑TTTAA‑CC‑‑CACCCCAGCGGGGGGAAACCCTCGCCCGGTTCTTTCCCCCG‑atattcaggtgtcctcccc                                                                                                            >  1:470269/1‑136 (MQ=255)                                       gccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCGCGTT‑TCCCCG‑CCCGTGC‑TTTTA‑AC‑GTAACCAGGCGGGGAGTAAACCCCCCCCGCTTCTTTCCCCAG‑ATATC‑GGGATGACAcccc                                                                                                            >  1:1245999/1‑150 (MQ=255)                                           gggTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCccgccg                                                                                                        <  2:177832/150‑1 (MQ=255)                                              tttGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCGCGGT‑TCGCCG‑CCCGGGC‑TTTTA‑CC‑CGACCCAGGCGGGGAGGAAACTCCCCCCGATTCTTTTCCCCG‑ATATC‑GAGATGCCCGCCCGCCGtt                                                                                                      >  1:593261/1‑149 (MQ=255)                                                      tCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCCCGGT‑TCGCCG‑CCCGTGC‑CTTTA‑CC‑CTACCCCGGCGGGGCGGAACCTCCCCCCCGTTCTTTTCCCCC‑ATATC‑GAGATTCCCACccgcccctaaaaccaaa                                                                                             >  1:53446/1‑138 (MQ=255)                                                       cccagcccagGGGGGCTCCGCTCCCCCCCAGCAGTGGGCGGG‑TTTGCTCGCTTC‑CCCCCC‑CCCGTTT‑TTTAA‑CC‑GTCCCCGGGGGGGAAGTACCCTCCGCCCGATTCTTCCCCCAG‑ATAGG‑AAGAGGCCAGCCCGCCGTTAAGCCaaaa                                                                                             <  2:998723/149‑1 (MQ=255)                                                        ccAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGGT‑CCGCCG‑CCCCGGC‑TTTAA‑CC‑CTACCCAGGCGGGCCGCAACCTCCGCCCGCTTCTTTTCCCCG‑ATATCGGGGAGGCCGCCCCGCCG‑TAAGCCaaaa                                                                                             >  1:603223/1‑148 (MQ=255)                                                        ccAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTTTCGCGGT‑TCCCCG‑CCCGCGCTTTTAC‑CC‑GAACCCCGCGGGGCGGAAACC‑CCCCCCCACTCTTTTCCCCG‑CTATC‑GCGATGCCAGCCCCCCCTTTAGGCcaaa                                                                                             >  2:117098/1‑148 (MQ=255)                                                           gTACAGGCGGGCTACGCTCCCAGCAACCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTCCCCAGGCGGGCAGTAACCCCCGCCCGTTTCTTCCCCCAA‑ATATC‑GAGATGCCGGCCCGCCGTTAAGCCAAAAGTAcc                                                                                        <  1:660192/150‑1 (MQ=255)                                                              cAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑CTTGCTCGCGTT‑TCGCCG‑CCCGTGC‑TTTTA‑CC‑GTACCCAGGCGGGCAATAAACTCCGCCCGATTCTTTTCCCAT‑ATATC‑GGGATCTCAGCCCCCCGTTAAGCCCAAAGTACCGtt                                                                                     >  1:154073/1‑150 (MQ=255)                                                               aGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTgg                                                                                   >  1:57223/1‑151 (MQ=255)                                                                ggCGGGCTCCGCCGCCAGCCAGCAATTGGCGGT‑TTTGCTCGCGTC‑CCGCCG‑CCCGTGC‑TTTAC‑CC‑GTCCCCAGGGGGGCAGTACCCCCCCCCCGATTCTTCCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCg                                                                                       <  2:85014/146‑1 (MQ=255)                                                                ggCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTTCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTgg                                                                                   >  1:187598/1‑150 (MQ=255)                                                                   gggCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAAACTCCGCCCGATTCTTTTCCCAG‑ATATC‑GAGATGCCAGCCCCCCGTTAAGCCCAAAGTAACGTTgg                                                                                   >  2:125766/1‑147 (MQ=255)                                                                    gtttgcgttGCCAGCCATTATTGGGGGGG‑TTGGTGCGCCTC‑CCCCCC‑CCCATGT‑TTTAA‑CC‑CCCCGCGGGCGGGCATTACCCCCCCCCCGATTCTTTCCCGAT‑ATACG‑GGGGTCGATGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTa                                                                               <  1:938867/142‑1 (MQ=255)                                                                    ggCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGgt                                                                                  >  1:829637/1‑147 (MQ=255)                                                                        aCGCGGCCAGCCAGCAATGGGCGGT‑TTTGCCCGGTTT‑CCCCCC‑CCCGTGC‑TTTAA‑CC‑GTACCGGGGCGGGGAGTACCCCCCCCCCGATTCTTTCCCAAG‑AAATG‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTAcc                                                                             <  1:518812/148‑1 (MQ=255)                                                                         cGCTGCCAGCCACCAATTGGCGGG‑TTTGCTCGCTTT‑CCCCCC‑CCCGTGC‑TTTAA‑CC‑GTCCCCAGGCGGGCAGTACCCCCCCCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCAc                                                                           <  2:995898/149‑1 (MQ=255)                                                                            tGCCAGCCAGCAATTGGCGGG‑GTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAACCAAAAGTACCGTTTGTGTACCCCGGGcc                                                                      >  1:739709/1‑151 (MQ=255)                                                                              ccagccagCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAC‑CC‑GTACCCAGGCGGGCAGTAACCCCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCgg                                                                    <  1:645872/151‑1 (MQ=255)                                                                               cagccagcAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCgg                                                                    >  1:513920/1‑150 (MQ=255)                                                                                  ccagcATTGGGCGGT‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGT‑TTTAA‑CC‑GTCCCCAGGCGGGCAGTACCCCCCGCCCGATTCTTCCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGt                                                                <  1:1236009/151‑1 (MQ=255)                                                                                  ccagcAATTGGGGGT‑TTGGCCCGCGTC‑CCGCCC‑CCCGTGC‑TTTAC‑CC‑GTCCCCGGGGGGGCAGTACCCCCCCCCCGTTTCTTCCCCCAA‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCg                                                                 <  2:801918/150‑1 (MQ=255)                                                                                  ccagcAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTTC‑TTTAC‑CC‑GTCCCCAGGCGGGCAGTACCCCCCGCCCGTTTCTTCCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGt                                                                <  2:69964/151‑1 (MQ=255)                                                                                  ccagcAATGGGCGGT‑TTTGCTCGCGTC‑CCGCCG‑CCCGTGT‑TTTAC‑CC‑GTCCCCAGGCGGGCAGTACCCTCCCCCCGATTCTTCCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTc                                                                  <  1:969103/149‑1 (MQ=255)                                                                                  ccagcAATGGGCGGG‑TTTGCTCGCTTC‑CCCCCC‑CCGGTGT‑TTAAC‑CC‑GTCCCCGGGGGGGCAGTACCCCCCGCCCGTTTCTTCCCCCAG‑ATATC‑GAGAGGCCGCCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGt                                                                <  2:279435/151‑1 (MQ=255)                                                                                     agaaTTGGGGGGTTTTGCTC‑CGTTCCCGCCG‑CCCGTGT‑TTTAA‑CC‑GCCCCCAGGCGGCAAGTACCCCCCGCCCGATTCTTTCCCGAA‑ATATC‑GAGATGCCACCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTc                                                                  <  2:87230/145‑1 (MQ=255)                                                                                         ttGGCGGG‑TTTGCTCGCGGT‑CCCCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCCGGAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGGACCTGc                                                          >  2:429514/1‑150 (MQ=255)                                                                                           ggCGGG‑TTCGCTTGCTTC‑CCGCCC‑CCCGTG‑‑TTTAA‑CCTTTACCAATGCGGGTGGTACCCCCCGCCCGATTCTTCCCCCAA‑ATAAA‑GAGCTGCCGCCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCgg                                                        <  1:786649/150‑1 (MQ=255)                                                                                               gg‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCgg                                                    >  2:770441/1‑150 (MQ=255)                                                                                                     gCTCGCGTC‑CCGCCG‑CCCGTTT‑TTAAC‑CC‑ACACCCAGGCGGGCAGAACCCCCCGCCCGTTTCTTTCCCCAG‑ATATC‑GAAACCCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCg                                                <  2:229570/149‑1 (MQ=255)                                                                                                         gcgTC‑CCGCCC‑CCCGTGT‑TTTAC‑CC‑TTACCCAGGCGGGCAGTACCCCCCCCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGt                                               <  1:310034/146‑1 (MQ=255)                                                                                                             tcccgccc‑CCCGTGC‑TTTAC‑CC‑GCCCCGGGGGGGGCAGTACCCCCCGCCCGTTTCTTCCCCAAA‑ATACC‑GAGATGCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGc                                        <  2:880541/148‑1 (MQ=255)                                                                                                             t‑CCCCCC‑CCCGGTC‑TTTAACCC‑GTCCCAGGGCGGGAAGTACCCCCCCCCCGTTTCTTCCCCCAG‑ATACC‑GGGATCCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGtt                                              <  2:1138635/144‑1 (MQ=255)                                                                                                               ccgccg‑CCCGTGT‑TTTAC‑CC‑ATACCCGGGCGGGAAGTACCCCCCGCCCATTTCTTCCCCCAG‑AAACC‑GAGATGCCACCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCag                                           <  2:684336/145‑1 (MQ=255)                                                                                                                 gccg‑ccCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACg                                   >  1:480431/1‑151 (MQ=255)                                                                                                                 gccg‑ccCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACg                                   >  2:1023895/1‑151 (MQ=255)                                                                                                                  ccg‑ccCGTGT‑TTTAC‑CC‑GTCCCCAGGCGGGCAGTACCCCCCGCCCTTTTCTTCCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACgg                                  <  2:394908/151‑1 (MQ=255)                                                                                                                      cccGTTC‑TTTAC‑CC‑CTCCCCGGGCGGGCAGTACCCCCCGCCCTTTTCTTCCCCCAA‑ATATC‑GAGATGCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGcc                                <  2:578737/150‑1 (MQ=255)                                                                                                                       ccGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGcccc                              >  2:300455/1‑151 (MQ=255)                                                                                                                         gTGC‑TTTAA‑CC‑GTCCCCAGGGGGGAAGAACCCCCCCCCCGATTCTTTCCCCAA‑ATATC‑GAGATGCCATCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCa                             <  1:178136/150‑1 (MQ=255)                                                                                                                         gTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCAt                            >  2:328591/1‑151 (MQ=255)                                                                                                                            c‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTa                          >  1:924860/1‑150 (MQ=255)                                                                                                                             tgctttaccgtcccccAGGCGGCAACAACCCCCCCCCCGATTC‑TTCCCCAGAATATC‑GAGATCCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCa                             <  2:474665/136‑1 (MQ=255)                                                                                                                                tAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGcc                      >  1:856403/1‑151 (MQ=255)                                                                                                                                  accc‑GTCCCCAGGGGGGCAGTACCCCCCGCCCGTTTCTTTCCCCAG‑ATATC‑GAGAGGCCACCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGc                       <  2:362245/147‑1 (MQ=255)                                                                                                                                   acccgagcccGGGGGGGCATTACCCCCCCCCATATTCTTCCCCCAG‑ATACC‑GGGATCCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAg                        <  2:809402/141‑1 (MQ=255)                                                                                                                                    cc‑GTCCCCGGGCGGGCAGTACCCCCCGCCCGATTTTTCCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTc                   <  2:203923/151‑1 (MQ=255)                                                                                                                                    cc‑GTCCCCAGGCGGGCAGTACCCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCt                     <  1:987316/149‑1 (MQ=255)                                                                                                                                    cc‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTc                   >  1:23113/1‑151 (MQ=255)                                                                                                                                       gTACCCAGGGGGGGAGTACCCCCCGCCCTTTTCTTTCCCAAG‑ATATC‑GAAATCCCACCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCt                  <  2:660376/150‑1 (MQ=255)                                                                                                                                       gTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTg                 >  2:621822/1‑151 (MQ=255)                                                                                                                                          cccAGGCGGGCACTACCCTCCCCCCGTTTCTTCCCCAAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGc                <  2:1287054/149‑1 (MQ=255)                                                                                                                                             aGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGAc            >  2:97840/1‑150 (MQ=255)                                                                                                                                              ggggggAAGTACCCCCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACa           <  1:1090330/147‑1 (MQ=255)                                                                                                                                              ggCGGGAAGAAACCCCCGCCCGATTCTTCCCCAAG‑ATACG‑GAAAGGCAAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCt                  <  2:1277372/143‑1 (MQ=255)                                                                                                                                               gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAtt         >  2:951811/1‑151 (MQ=255)                                                                                                                                                cGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAttt        >  2:221686/1‑151 (MQ=255)                                                                                                                                                 gggCAGTACCCCCCGCCCTATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAtt         <  1:567146/149‑1 (MQ=255)                                                                                                                                                 gggAAGTACCCTCCCCCCGTTTCTTCCCCCAG‑ATACC‑GAGATCCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAttt        <  2:871198/150‑1 (MQ=255)                                                                                                                                                      gTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGcc  >  2:1221615/1‑151 (MQ=255)                                                                                                                                                            |                                                                                                                                                   GCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCGTT‑CCGCCG‑CCCGTGC‑TTTAA‑CC‑GTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAG‑ATATC‑GAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCC  >  NC_000913/2228126‑2228419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑