Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,2680TA40.0% 94.4 / 36.7 71L24F (TTA→TTTemrKmultidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (28/14);  new base A (0/28);  total (28/43)
Fisher's exact test for biased strand distribution p-value = 1.97e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483118‑2483416
                                                                                                                                                           |                                                                                                                                                    
gTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                                      <  1:459399/151‑1 (MQ=255)
 ttCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                                      >  2:705919/1‑150 (MQ=255)
  tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAAATCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                                      <  2:950998/149‑1 (MQ=255)
   cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAAtaaataaa                                                                                                                                                   <  2:1218003/151‑1 (MQ=255)
     tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAAc                                                                                                                                                 >  1:406025/1‑151 (MQ=255)
       tCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt                                                                                                                                                >  2:1133180/1‑150 (MQ=255)
          ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg                                                                                                                                            >  2:1128755/1‑151 (MQ=255)
           tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGc                                                                                                                                           >  2:94294/1‑151 (MQ=255)
            aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc                                                                                                                                          >  1:1265647/1‑151 (MQ=255)
            aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc                                                                                                                                          <  2:1310554/151‑1 (MQ=255)
            aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGc                                                                                                                                           <  2:552754/150‑1 (MQ=255)
              gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCa                                                                                                                                         >  2:464613/1‑150 (MQ=255)
                  aaCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATaa                                                                                                                                     >  1:1174971/1‑150 (MQ=255)
                    cGACAGTGCCACTCCCGGATATTTGTGCAAAAATTGTATCTGCATTCCCCGTGACATAGGCTTCATCTGTACTAATCATTTCTTCAAATTCCATTGACCAATAGACAAAGGCCCTTGAAACCGCAAAAAATAAAACTACCGCCAATAAAGa                                                                                                                                  <  2:159269/151‑1 (MQ=255)
                      aCAGTGCCACTACCTGAGATTTGTGCAAAAATTGGATCGAAATTCCCGGAGACATAGGCGTCATTTGTACAAATCATGTCTTTTAATACCATTGACCAATGGGAAAAGGCCCCGAAAAGCGCAATAAATAAAACTACCGCCAATAAAGaaa                                                                                                                                <  1:1110859/151‑1 (MQ=255)
                      aCAGTGACACTCCCGGAGACTTGTGCAAAAATTGGATCTGCATTCCCCGTAACAGAGGCGTCATTTGTACTAATCATGTCTTCAAATTCCATGGACAAAGAGGAATAGGCCCGGAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGaa                                                                                                                                 <  1:915088/150‑1 (MQ=255)
                       cAGTGCCCCTCCCTGAGATTTGTGAAAAAATTGAATCTGCTTCCCCCGGGACATAGGCGTCATCTTTACTAATAATGTTTTCTAATCCCATGGCCAAAAAGGAAAAGGCCCCTGAAAACGAAAAAAATAAAACTACCGCCAATAAAg                                                                                                                                   <  2:799727/147‑1 (MQ=255)
                       cAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa                                                                                                                                >  2:88706/1‑150 (MQ=255)
                        aGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa                                                                                                                                >  1:40024/1‑149 (MQ=255)
                          tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGt                                                                                                                             >  2:1096725/1‑150 (MQ=255)
                               cTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc                                                                                                                       >  1:1098370/1‑151 (MQ=255)
                                 cccggagaTTTGTGCAGAAATTGGTTCTGCTTCCCCGGTGACATGGCGGTCATCTTTACTAACCATGTCTTATAATCCCATTAACCAATGGGTAGAGCCACCTAAAAACGAAATAAAAAAACCTCCCGCCAATAAAGAAAAGTATTttctt                                                                                                                     <  2:132532/147‑1 (MQ=255)
                                 aCCTGAGACTTGTGCAGAAATTGGATCTGCATGCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCACTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACTGCCAATAAAGAAAAGTATTttctt                                                                                                                     <  1:876070/151‑1 (MQ=255)
                                  ccTGAGACTTGTGAAGAAATTGGTTCTGCTTTCCCCTTTACAAAGGCGTCATCTGTAATAATCATGTCTTAAAATTCCATTGACCAATAGGAAAAGGCACCTAAAACCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                                     <  2:679259/150‑1 (MQ=255)
                                    tGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTg                                                                                                                  >  1:189415/1‑151 (MQ=255)
                                    tGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTAACATAGGCGTCATTTTTAATAATCATTTCTTCTAATTCCATTGACAAATAGGAATGGGCACCTAAAAACGAAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTttctt                                                                                                                     <  1:702955/148‑1 (MQ=255)
                                     gagcCTTGCGCAGAAATTGTATCTGCATCCCCCGTGACAGAGGCGTCTTCTGTACTACTCATTTCTTCTAATCCCATGGACCAATAGGCATAGCCCCCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTg                                                                                                                  <  1:99084/150‑1 (MQ=255)
                                     gagaCTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGt                                                                                                                 >  2:633646/1‑151 (MQ=255)
                                          ttGTGAAAAAATTGGATCTGCATTCCCCGTAACATAGGCGCCATCTGTACTAATCATTTCTTATAATTCCATTAACCAATAGGCATAGGCACCTAAAACCGAAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                                                <  2:756656/147‑1 (MQ=255)
                                              gCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCTTCTGTACTAATCATTTCTTCTAATTCCATTGACCAATAGGAATAGCCACCTAAAAACGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                          <  1:505587/149‑1 (MQ=255)
                                                aaaaaTTGTATGTACTTTCCCCGGGACATAGGTTTCTCTTGTCTTAATCTTGTCTTAAAATCCCATTACCCAAAAGCAATAGCCCCTAAAAACCCAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                          <  1:486800/145‑1 (MQ=255)
                                                aaaaaTTGTACTTGCATTCCCCGTGACATAGGCGTCATTTGTACTAATCATGTTTTCTAATTCCATTGCCCAATAGACAAAGGCACTTAAAAACCAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt                                                                                                        <  2:1100200/147‑1 (MQ=255)
                                                aGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt                                                                                                      >  1:186844/1‑151 (MQ=255)
                                                aGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt                                                                                                      >  2:575554/1‑151 (MQ=255)
                                                  aaaTGGGATCCGCATTCCCGGGCACAAGGGCGTCATTTGATCAAATCATGTCTTCTAATCCACTTCCCCAATTCAAATGGCAAAATAAAAACCAAATAAAAAAAACCACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt                                                                                                      <  2:738633/149‑1 (MQ=255)
                                                   aaTTGGATCTGCATTCCCCGTGACATAGGCGTCATTTGTACTAATAATTTCTTAAAATTCCATTGCCAAATAGGAAAAGCCACCTAAAAACAAAAAAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                   <  2:418392/151‑1 (MQ=255)
                                                   aaTTGGATCTGCATCCCCCGTAACAAAGGCGTCATTTGTACAAATAATGTCTTCAAATCCAATTAACCAATAGGAAAAGGCACCTAAAACCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                   <  2:1310442/151‑1 (MQ=255)
                                                         aTCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                                              >  2:1315908/1‑150 (MQ=255)
                                                          tCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt                                                                                            >  1:1317081/1‑151 (MQ=255)
                                                               atcccccGTAAAATAGGCGTAATTTTTACTAATCATGTCTAAAAATTCCATTAACAAAAGGGCATAGGCCCTTAAAAACCAAAAAAAAAATTCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTg                                                                                               <  2:988501/140‑1 (MQ=255)
                                                                tcccccGTGACATGGGCGTCATCTTTACTAATCATGTCTTCTAATTCCATTGCCAAATAGGCAAAGGCACCGAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTaa                                                                                         <  1:1164723/146‑1 (MQ=255)
                                                                  ccccGTGACAAAGGCGTCATCTATACTAATCATGTCTTCTAATTCCATTGCCAAATAGGCAAAGGCACCAAAAAACAAAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                    <  1:1112334/151‑1 (MQ=255)
                                                                   cccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                                   >  2:987588/1‑151 (MQ=255)
                                                                        gACATAGGCGTCATCTGCACTATTCATGTTTTCTATTCCCATTGCCCAATAGGCATAGGCCCCGGAAAACAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCAc                                                                               <  1:127362/150‑1 (MQ=255)
                                                                         aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                              >  2:1068429/1‑150 (MQ=255)
                                                                         aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                              >  1:1210090/1‑150 (MQ=255)
                                                                              ggCTTCATCTGTACTAATCATGTTTTTTAATTCCATTGAAAAAGAGATAGAGGCACTTGAAAACGTAAAAAAAAAAATTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtc                                                                        <  2:1245602/151‑1 (MQ=255)
                                                                              ggCATCATCTGTACTAATCTTGTCTTCAAATTCAATTAACAAATAGGCAAAGGCACCTGAAAGCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                         <  2:312765/150‑1 (MQ=255)
                                                                               gCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtct                                                                       >  1:202114/1‑151 (MQ=255)
                                                                                 gtgatcttaacaaaTCATTTCTTCTAACCCATTAAACCAAAGGCAATAGGCACCTAAAACCCAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctc                                                                      <  1:859974/138‑1 (MQ=255)
                                                                                   cATCTGTACTAATCATTTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctctc                                                                    <  2:481369/150‑1 (MQ=255)
                                                                                   cATCTGAACTAATCATGTCTTATATCCCCATGCACCAAAGGCAAAAGTCCCCGAAAACCGAAAAAAAAAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctct                                                                     <  2:129068/149‑1 (MQ=255)
                                                                                           caaattattctttatAATTCCATTACCCAACAGGCATAGACACCTAAAAGCTAAATAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAt                                                                  <  2:40024/130‑1 (MQ=255)
                                                                                             aaTCATGTTTTCTAATCCCATTGACAAATAGGCATGGGCCCCAAAAACCAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAttt                                                                <  1:201257/144‑1 (MQ=255)
                                                                                              atcatcTCTTACAATCCCATGACCGAATGGGCAAGGCACCCTAAAAACGCAAAAAAAAAACCTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                           <  1:143702/148‑1 (MQ=255)
                                                                                                 aTGTCTTCTAATTCCATTGACCAATAGGCAAAGCCACCTAAAAACGAAAAAAATAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                      <  2:442668/150‑1 (MQ=255)
                                                                                                 aTGTCTTAAAATCCCATGGAACAAAAGACATAGGCACCTAAAAACTAAAAAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                       <  2:622011/149‑1 (MQ=255)
                                                                                                      ttctAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                                                 <  1:562156/150‑1 (MQ=255)
                                                                                                       tctAATTCCATTGACAAATAGGCATAGGCCCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAt                                                  <  1:252896/148‑1 (MQ=255)
                                                                                                        ctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                                              >  2:430551/1‑151 (MQ=255)
                                                                                                           aTTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                                            >  1:970101/1‑150 (MQ=255)
                                                                                                               cATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                        >  2:451471/1‑150 (MQ=255)
                                                                                                                aTTGACCAATAGGCATAGGCCCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaa                                           <  1:306161/146‑1 (MQ=255)
                                                                                                                 ttGACAAATAGGCAGAGGCACCTAAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaat                                          <  1:243022/146‑1 (MQ=255)
                                                                                                                       caaatggaaataggcaCTTAAAAACCCAATAAAGAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCGCTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                        <  1:996265/135‑1 (MQ=255)
                                                                                                                        aaTAGGCATAGGCACCTAAAAACGCAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag                               <  1:981751/151‑1 (MQ=255)
                                                                                                                             gcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcat                          >  2:1231917/1‑151 (MQ=255)
                                                                                                                                ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattc                        >  1:363772/1‑151 (MQ=255)
                                                                                                                                 atagcccccaaaaaaCAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcatt                         <  1:893793/139‑1 (MQ=255)
                                                                                                                                  aggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcat                      >  1:670833/1‑151 (MQ=255)
                                                                                                                                        ccaaaaacCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                 <  1:705943/147‑1 (MQ=255)
                                                                                                                                        ccaaaaacCGCAAAAAAAAAACTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt                  <  2:1065597/146‑1 (MQ=255)
                                                                                                                                        ccaaaaaaCGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                 <  1:464628/147‑1 (MQ=255)
                                                                                                                                            aaaaCGAAATAAAAAAAACAACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCatat             <  1:983253/151‑1 (MQ=255)
                                                                                                                                               aCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCa           >  1:1020926/1‑150 (MQ=255)
                                                                                                                                                      ataaataaAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac      >  2:180855/1‑150 (MQ=255)
                                                                                                                                                      ataaaaaaAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAAcaca     <  1:902097/151‑1 (MQ=255)
                                                                                                                                                         aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTg  >  2:512398/1‑151 (MQ=255)
                                                                                                                                                         aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTg  <  2:186838/151‑1 (MQ=255)
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GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483118‑2483416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: