Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 700,980 G→C Y205* (TAC→TAG nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913700,9800GC98.8% 327.1 / ‑3.4 83Y205* (TAC→TAGnagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (39/43);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGTCAACAAGC  >  NC_000913/700836‑701127
                                                                                                                                                |                                                                                                                                                   
cAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGc                                                                                                                                               <  2:329170/151‑1 (MQ=255)
cAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGc                                                                                                                                               >  2:156493/1‑151 (MQ=255)
 aGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCt                                                                                                                                              <  1:139337/151‑1 (MQ=255)
   cGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTcc                                                                                                                                            >  1:554360/1‑151 (MQ=255)
   cGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTcc                                                                                                                                            <  1:801213/151‑1 (MQ=255)
    ggTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCcg                                                                                                                                           <  1:257801/151‑1 (MQ=255)
      ttCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCcgcc                                                                                                                                         >  1:340343/1‑151 (MQ=255)
        cGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCcgcc                                                                                                                                         >  1:1316071/1‑149 (MQ=255)
          aCCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCcgccagcg                                                                                                                                     <  2:238078/151‑1 (MQ=255)
             tGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCcgccagcgcc                                                                                                                                   >  2:1000002/1‑150 (MQ=255)
               aaTATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCcgccagcgcc                                                                                                                                   >  2:1182234/1‑148 (MQ=255)
                  atGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTa                                                                                                                             <  2:163683/151‑1 (MQ=255)
                     gCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACgg                                                                                                                          <  1:80671/151‑1 (MQ=255)
                      ccAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACCAGTGCTCCGCCAGCGCCAGACTACGGa                                                                                                                         <  1:453869/151‑1 (MQ=255)
                       cAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGat                                                                                                                        >  1:1319878/1‑151 (MQ=255)
                       cAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGat                                                                                                                        >  1:167248/1‑151 (MQ=255)
                        aaTTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGata                                                                                                                       <  2:783070/151‑1 (MQ=255)
                              aCCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGt                                                                                                                   >  2:489550/1‑149 (MQ=255)
                                cGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGAcc                                                                                                               <  2:948519/151‑1 (MQ=255)
                                   cgttgccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCaa                                                                                                             <  2:235770/150‑1 (MQ=255)
                                    gttgccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACg                                                                                                           >  1:805116/1‑151 (MQ=255)
                                    gttgccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACg                                                                                                           >  1:467900/1‑151 (MQ=255)
                                    gttgccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACg                                                                                                           >  1:43024/1‑151 (MQ=255)
                                      tgccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGa                                                                                                          >  2:1075887/1‑150 (MQ=255)
                                      tgccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGa                                                                                                          >  2:995511/1‑150 (MQ=255)
                                       gccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGaaa                                                                                                        <  2:947252/151‑1 (MQ=255)
                                        ccgttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGaaa                                                                                                        <  1:433012/150‑1 (MQ=255)
                                           ttgcGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAgg                                                                                                    >  1:807661/1‑151 (MQ=255)
                                                gCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACt                                                                                               >  1:433508/1‑151 (MQ=255)
                                                 ccAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACtt                                                                                              <  1:143096/151‑1 (MQ=255)
                                                  cAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACttt                                                                                             >  2:1042823/1‑151 (MQ=255)
                                                  cAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACttt                                                                                             <  2:676875/151‑1 (MQ=255)
                                                      aaaaaTGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTaaa                                                                                          >  1:2773/1‑150 (MQ=255)
                                                       aaacTGCGCCCGTTAGAGATGGTCCCGACCCCCGTTCCGCGATGGAAGCGAACCAGAATGGAGTCTTCGCAATCCTGCATTGCACCGAACTAGTGCTCCGCCAGCAGCAGAATACGGATATCGTGACCAACGAAACAGGTCACTTTaaa                                                                                          <  2:141929/149‑1 (MQ=255)
                                                         aaTGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGt                                                                                      >  2:754437/1‑151 (MQ=255)
                                                           tGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGttc                                                                                    >  2:908792/1‑151 (MQ=255)
                                                                ccGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTcc                                                                                >  1:377633/1‑150 (MQ=255)
                                                                ccGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCa                                                                               <  2:355847/151‑1 (MQ=255)
                                                                 cGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTcc                                                                                >  1:270174/1‑149 (MQ=255)
                                                                    tAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCagag                                                                            <  1:1170338/150‑1 (MQ=255)
                                                                    tAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGc                                                                           >  2:289214/1‑151 (MQ=255)
                                                                           aaTCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTAcc                                                                    >  1:435432/1‑151 (MQ=255)
                                                                            aTCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTAc                                                                     >  2:1147670/1‑149 (MQ=255)
                                                                                cGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGccc                                                               >  2:591107/1‑151 (MQ=255)
                                                                                cGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGccc                                                               <  1:527316/151‑1 (MQ=255)
                                                                                cGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGccc                                                               >  2:355983/1‑151 (MQ=255)
                                                                                 ggCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGcccc                                                              <  1:1042877/151‑1 (MQ=255)
                                                                                    cccGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAg                                                           <  1:995563/151‑1 (MQ=255)
                                                                                     ccGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAg                                                           >  2:1144939/1‑150 (MQ=255)
                                                                                         ttCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGtttt                                                       <  2:497699/150‑1 (MQ=255)
                                                                                            cgcgATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTAc                                                   >  1:397473/1‑151 (MQ=255)
                                                                                              cgATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTAcc                                                  <  1:256562/150‑1 (MQ=255)
                                                                                              cgATGGACACGCACCAAAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCGTCTACCAGCCCCCAGTTTTCTACCt                                                 <  1:1094211/151‑1 (MQ=255)
                                                                                               gATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTg                                                <  1:1212781/151‑1 (MQ=255)
                                                                                                aTGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTg                                                >  2:99296/1‑150 (MQ=255)
                                                                                                aTGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGa                                               <  2:1325510/151‑1 (MQ=255)
                                                                                                  ggACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGaa                                              <  1:1195296/150‑1 (MQ=255)
                                                                                                  ggACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGaa                                              <  2:330375/150‑1 (MQ=255)
                                                                                                     cacGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAatat                                           <  1:162937/150‑1 (MQ=255)
                                                                                                     cacGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAatat                                           >  1:115836/1‑150 (MQ=255)
                                                                                                        gCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCgg                                       >  2:1084696/1‑151 (MQ=255)
                                                                                                          aCCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCCGCCCCCAGTTTTCTACCTGAATATGCGGc                                      >  1:739850/1‑150 (MQ=255)
                                                                                                             aGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGt                                  >  2:1209805/1‑151 (MQ=255)
                                                                                                             aGAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGt                                  <  1:988870/151‑1 (MQ=255)
                                                                                                              gAATGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTa                                 <  2:649413/151‑1 (MQ=255)
                                                                                                               aaTGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTaa                                <  2:345530/151‑1 (MQ=255)
                                                                                                                aTGGAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTa                                 <  1:497902/149‑1 (MQ=255)
                                                                                                                  ggAGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGa                             <  2:1204478/151‑1 (MQ=255)
                                                                                                                    aGTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAAt                           <  2:449693/151‑1 (MQ=255)
                                                                                                                     gTCTTCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAAtt                          <  1:801563/151‑1 (MQ=255)
                                                                                                                        ttCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAAtttt                        >  1:380512/1‑150 (MQ=255)
                                                                                                                         tCGCAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTg                       <  1:1035220/150‑1 (MQ=255)
                                                                                                                            cAATCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGc                   <  1:1075946/151‑1 (MQ=255)
                                                                                                                            cAAACCTGCCTTGCACCGAACTAGTGCACCGCCAGAGCCAGACTACGGATAGCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTATACCTGAATATACGGCATGTAATGAAtgt                         <  2:71284/145‑3 (MQ=255)
                                                                                                                             aaTCCTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCt                  >  1:1296531/1‑151 (MQ=255)
                                                                                                                                ccTGACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTc               >  1:762908/1‑151 (MQ=255)
                                                                                                                                   gACTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCgg            <  2:476846/151‑1 (MQ=255)
                                                                                                                                    aCTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCggg           <  2:358497/151‑1 (MQ=255)
                                                                                                                                     cTTGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGt          >  1:302942/1‑151 (MQ=255)
                                                                                                                                       tGCACCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGTc         <  2:702598/150‑1 (MQ=255)
                                                                                                                                          aCCGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGTcaac      >  1:958920/1‑150 (MQ=255)
                                                                                                                                           ccGAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGTcaacaa    <  1:289222/151‑1 (MQ=255)
                                                                                                                                             gAACTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGTCAACAAGc  >  1:911075/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCCGTTAGAGATAATCCCGGCCCCGGTTCCGCGATGGACACGCACCAGAATGGAGTCTTCGCAATCCTGACTTGCACCGAAGTAGTGCTCCGCCAGCGCCAGACTACGGATATCGTGACCAACGAAACAGGTCACTTTAAAACGTTCTTCCAGAGCTTCTACCAGCCCCCAGTTTTCTACCTGAATATGCGGCATGTAATGAATTTTGCCGCTGTCCGGGTCAACAAGC  >  NC_000913/700836‑701127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: