Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,313	T→A	V286E (GTG→GAG)	mdtK →	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,313	0	T	A	100.0%	341.6 / NA	91	V286E (GTG→GAG)	mdtK	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (55/36);  total (55/36)

ACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGG  >  NC_000913/1744167‑1744456                                                                                                                                                   |                                                                                                                                                aCTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCtt                                                                                                                                             <  1:786040/151‑1 (MQ=255)        cAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATg                                                                                                                                       <  2:875470/150‑1 (MQ=255)         aaCTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTc                                                                                                                                     <  2:725375/151‑1 (MQ=255)            tCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCt                                                                                                                                  >  1:1210530/1‑151 (MQ=255)             cGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCt                                                                                                                                  >  2:1190884/1‑150 (MQ=255)             cGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTg                                                                                                                                 <  1:472256/151‑1 (MQ=255)                tttGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTggcg                                                                                                                              >  1:992890/1‑151 (MQ=255)                 ttGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTggcg                                                                                                                              <  2:317157/150‑1 (MQ=255)                 ttGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGAAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTggcgg                                                                                                                             >  2:1060966/1‑151 (MQ=255)                   gCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTggcggc                                                                                                                            >  2:1233738/1‑150 (MQ=255)                          gcgcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTa                                                                                                                     <  1:668876/150‑1 (MQ=255)                          gcgcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCCGCAGCGGTa                                                                                                                     >  1:443349/1‑150 (MQ=255)                            gcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                                                                                                                  <  1:792027/151‑1 (MQ=255)                              tGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTa                                                                                                                 >  1:567356/1‑150 (MQ=255)                                gCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATcc                                                                                                              <  1:973192/151‑1 (MQ=255)                                gCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATcc                                                                                                              >  2:113977/1‑151 (MQ=255)                                  aCTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCcgc                                                                                                            <  2:573402/151‑1 (MQ=255)                                  aCTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCcgc                                                                                                            <  2:1093103/151‑1 (MQ=255)                                     gTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTa                                                                                                         <  2:96925/151‑1 (MQ=255)                                       tCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAgg                                                                                                       >  1:1143787/1‑151 (MQ=255)                                       tCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAg                                                                                                        <  1:151949/150‑1 (MQ=255)                                        cTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGt                                                                                                      >  2:1253704/1‑151 (MQ=255)                                         tttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGtt                                                                                                     >  1:1277162/1‑151 (MQ=255)                                         tttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGtt                                                                                                     >  1:1156873/1‑151 (MQ=255)                                         tttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGAATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCAGCGTAGGt                                                                                                      >  1:866688/1‑150 (MQ=255)                                          ttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAgg                                                                                                       <  1:628007/148‑1 (MQ=255)                                          ttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGtt                                                                                                     >  1:775668/1‑150 (MQ=255)                                           tGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTa                                                                                                    <  1:105530/150‑1 (MQ=255)                                            gAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc                                                                                                  <  2:832739/151‑1 (MQ=255)                                            gAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc                                                                                                  >  2:1193387/1‑151 (MQ=255)                                             aaGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCg                                                                                                 >  1:879194/1‑151 (MQ=255)                                              aGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGt                                                                                                >  1:151645/1‑151 (MQ=255)                                                   cacTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTggg                                                                                           >  2:695718/1‑151 (MQ=255)                                                   cacTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTggg                                                                                           >  2:94135/1‑151 (MQ=255)                                                       gTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTc                                                                                         >  1:639067/1‑149 (MQ=255)                                                             cgtcgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGGCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTc                                                                                  >  1:1112567/1‑150 (MQ=255)                                                              gtcgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCa                                                                                 >  1:604556/1‑150 (MQ=255)                                                                cgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACg                                                                              >  2:1173803/1‑151 (MQ=255)                                                                 gtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGCACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGc                                                                             >  2:1014085/1‑151 (MQ=255)                                                                 gtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGc                                                                             <  1:331030/151‑1 (MQ=255)                                                                  tGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCt                                                                            >  1:184365/1‑151 (MQ=255)                                                                       ctGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGa                                                                         >  2:834133/1‑149 (MQ=255)                                                                          ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgcgc                                                                    >  1:407916/1‑151 (MQ=255)                                                                            aGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCa                                                                   >  2:1143730/1‑150 (MQ=255)                                                                             gtgtCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCaa                                                                  <  1:503413/150‑1 (MQ=255)                                                                              agtCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAAc                                                                >  2:1030556/2‑151 (MQ=255)                                                                                  tcCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCt                                                            >  2:271174/1‑151 (MQ=255)                                                                                   ccGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCg                                                              <  1:203561/148‑1 (MQ=255)                                                                                       tCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGcgcg                                                       >  2:544903/1‑151 (MQ=255)                                                                                       tCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGcgcg                                                       >  2:782796/1‑151 (MQ=255)                                                                                          gTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGAc                                                    >  2:291281/1‑151 (MQ=255)                                                                                          gTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGAc                                                    <  1:50192/151‑1 (MQ=255)                                                                                            aTTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCg                                                  >  1:882403/1‑151 (MQ=255)                                                                                                 tgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCtt                                             >  1:1088854/1‑151 (MQ=255)                                                                                                 tgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCtt                                             >  1:439854/1‑151 (MQ=255)                                                                                                 tgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCt                                              <  1:1126193/150‑1 (MQ=255)                                                                                                   aTGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAt                                           <  1:114218/151‑1 (MQ=255)                                                                                                   aTGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGATATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCACACCGCTGCGCGGACCGGGCTTAt                                           >  2:1079755/1‑151 (MQ=255)                                                                                                     gTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtg                                          <  1:92405/150‑1 (MQ=255)                                                                                                     gTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtg                                          <  1:245543/150‑1 (MQ=255)                                                                                                       cGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtg                                       >  2:446607/1‑151 (MQ=255)                                                                                                        gCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtgg                                      >  1:715997/1‑151 (MQ=255)                                                                                                         cAGGACACCAGATTGCCCTGAACTTTAGTTCTCTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtgg                                      <  1:834162/150‑1 (MQ=255)                                                                                                         cAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtggg                                     >  1:1088937/1‑151 (MQ=255)                                                                                                          aGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAACCCGCTGCTCGGACCGGGCTTATggtgggt                                    <  1:1309348/151‑1 (MQ=255)                                                                                                          aGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtggg                                     >  2:990162/1‑150 (MQ=255)                                                                                                           ggACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtgggtg                                   <  2:117423/151‑1 (MQ=255)                                                                                                             acacCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTc                                 >  1:275372/1‑151 (MQ=255)                                                                                                              cacCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTc                                 >  1:1165608/1‑150 (MQ=255)                                                                                                                 cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGt                              >  2:1268831/1‑150 (MQ=255)                                                                                                                 cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTa                             <  2:409138/151‑1 (MQ=255)                                                                                                                  aGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGt                              >  1:1214560/1‑149 (MQ=255)                                                                                                                   gATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATg                           >  1:1176986/1‑151 (MQ=255)                                                                                                                    aTTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATgg                          <  1:1050781/151‑1 (MQ=255)                                                                                                                    aTTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATgg                          <  2:848261/151‑1 (MQ=255)                                                                                                                      tGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGc                         >  1:492831/1‑150 (MQ=255)                                                                                                                       gCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCa                        <  1:838837/150‑1 (MQ=255)                                                                                                                        cccTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACGATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAAc                      <  1:1079814/151‑1 (MQ=255)                                                                                                                            gAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTg                  <  1:606466/151‑1 (MQ=255)                                                                                                                            gAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTg                  >  2:132684/1‑151 (MQ=255)                                                                                                                            gAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTg                  <  2:604535/151‑1 (MQ=255)                                                                                                                              aCTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGAc                >  2:123629/1‑151 (MQ=255)                                                                                                                                tttAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACgg              >  1:911529/1‑151 (MQ=255)                                                                                                                                 ttcgTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACg               >  2:752866/4‑149 (MQ=255)                                                                                                                                   aGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCa           <  1:210957/151‑1 (MQ=255)                                                                                                                                   aGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCa           <  1:443443/151‑1 (MQ=255)                                                                                                                                      tCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAttt        >  1:574852/1‑151 (MQ=255)                                                                                                                                        aCTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCGGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGAGCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAtttt       >  2:283473/1‑150 (MQ=255)                                                                                                                                        aCTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTc      >  1:54803/1‑151 (MQ=255)                                                                                                                                           aaTGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACg   <  1:4476/151‑1 (MQ=255)                                                                                                                                             tGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTTTGGTGGGTGTCTGTATGGCAACCCTGACGGCCAtttttatgg  >  2:425033/1‑145 (MQ=255)                                                                                                                                                   |                                                                                                                                                ACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGG  >  NC_000913/1744167‑1744456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑