Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,578,290	0	A	C	42.6%	114.9 / 14.3	68	V146G (GTG→GGG)	gntR	d‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (32/7);  new base C (0/29);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 7.02e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGC  >  NC_000913/3578141‑3578436                                                                                                                                                       |                                                                                                                                                    tgatAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTccac                                                                                                                                                    >  1:614846/1‑151 (MQ=255)        aCGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑gtg                                                                                                                                             <  2:219631/150‑3 (MQ=255)        aCGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑ggg                                                                                                                                             >  2:108442/1‑150 (MQ=255)        aCGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑ggg                                                                                                                                             >  2:886633/1‑150 (MQ=255)        aCGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGa                                                                                                                                            <  1:99661/151‑1 (MQ=255)             cgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATAc                                                                                                                                        <  2:1045143/150‑1 (MQ=255)              gtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATAccc                                                                                                                                      >  1:1133565/1‑151 (MQ=255)                cgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCg                                                                                                                                     <  1:1027704/150‑1 (MQ=255)                cgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGc                                                                                                                                    <  1:419186/151‑1 (MQ=255)                   gaCGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGGGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATCCCCGCCa                                                                                                                                  <  1:605898/150‑1 (MQ=255)                      gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTc                                                                                                                              >  2:375587/1‑151 (MQ=255)                      gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTCCCCCCAC‑GGGAATACCCGCCACtt                                                                                                                               <  1:239497/150‑1 (MQ=255)                           ccGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAatc                                                                                                                          >  2:1237718/1‑150 (MQ=255)                                 tAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCttt                                                                                                                    >  1:845789/1‑150 (MQ=255)                                     cAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAgg                                                                                                                >  1:100560/1‑150 (MQ=255)                                     aattgtgGCGATCCCCGCGAGAAAAAATGGCAGTGGTCATCGGGGGTGCTCCTTAAAATTTAAAAAAC‑CCGACGGCAATATCAGGGCAGGGCGCTTTGTTGTCCATCAGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGt                                                                                                               <  2:790383/148‑1 (MQ=255)                                         ggggCGATGCCCGCGAGAAATAATGGCAGTGGTCATCTGGCGTGCTGCTTAAAAGTTAAAAAAC‑CCGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCATTCCCCCCAG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTg                                                                                                              <  2:965035/146‑1 (MQ=255)                                         ggggCGATCCCCGCGAGAAATAGGGGGAGTGTTCTTCTGGCGTGCTGCTAAAAATTTATAAAAC‑CCGGCGGCGATACAAGGGCAGGGCGACTTGCTGCCCACCATTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAgg                                                                                                                <  2:1096245/144‑1 (MQ=255)                                           ggCGTTCCCAGCGAGAAATTGTGGACGTGGTCAGCGGTCGTGCTGATTAAAATTTTTCAAAT‑CCGAGGGCGATACAATGGCAGGGCGCCTTGCTGTCAATCGTTTCCCCCAC‑GGAATTCCCCGCCACTTCAATCATCTTTAAGGTGcgcg                                                                                                          <  2:1113855/150‑1 (MQ=255)                                            gCGATCCCCGCGAACAATAAGGGCGGTGGTCTTCTGGCGTGCTGCTTAAATTTAATCAAAC‑CCGCCGGCAATATCAAGGCATGGCGTTTTGCTGTCCATCAGTCCCCCCCC‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGcgcg                                                                                                          <  2:794970/149‑1 (MQ=255)                                                tGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtg                                                                                                     >  2:60470/1‑150 (MQ=255)                                                 gCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCgcggc                                                                                                        >  1:209465/1‑146 (MQ=255)                                                  cccGCGAAAAATAGGGGCGGGGGTCATCTGGCGTCCTGCTTAAAAGTTAAAAAAA‑CCGACGGCAATAACAAGGCATGGCAACTTGCTCTACATCGGTCCCCCCAG‑GGTAATCCCCGCCACTTCAATCATCTTTAAGGTGcgcg                                                                                                          <  1:350865/143‑1 (MQ=255)                                                   ccGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTggg                                                                                                 >  1:196641/1‑151 (MQ=255)                                                                 ggCGGTGGTCATGTGGCGTCCTGCTCAAAAGTTCACAACC‑CCCCCGGAGATACGCGGGGAGGGCGTCTTGCTGCCCACCATTCCCCCCAG‑GGGAATAGCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa                                                                                     <  1:36835/149‑1 (MQ=255)                                                                 ggCGGTGGTCACCGGGCGTGCTCCTAAAAATTTAAAAAAC‑CCGACGGCATAATCAGGGCTGGGCCTCTTGTTGCCCATCTGTCCCCCCGG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa                                                                                     <  1:1245820/149‑1 (MQ=255)                                                                 gcgagcatgcATCTGGCGTTCT‑CTTCAAAGTTAAAAAAACCCGCGGGCGATATAAAGCCAGGGGCGTTTTCTACCCGCCGGTCCCCCCCC‑GGAAAGACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAgg                                                                                   <  2:978123/142‑1 (MQ=255)                                                                       ggTCGTCGGGCGGGCTGATTAAAATTAAAAAAAA‑CCGGGGAATATATAAAGGCATGCCAGTTTGCTGTCCATCAGTCCCCCGAA‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAg                                                                              <  1:677084/150‑1 (MQ=255)                                                                          cATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCg                                                                          >  1:1088387/1‑151 (MQ=255)                                                                          cATCGGGGGTGCTGCTAAAAAGTTAAAAAAC‑CCGACGGCTATATCAAGGCATGGCGTTTTGCTGCCCATCAGTCCCCCCAG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc                                                                           <  1:432558/150‑1 (MQ=255)                                                                          cATCGGGCGTGCGGCTTAAAATTTATAAAAC‑CCCCGGGCAAAATAAGGGCCGGCGGTCTTGCTGCCCGTAATTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc                                                                           <  1:473568/150‑1 (MQ=255)                                                                           aTCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                         >  1:35123/1‑151 (MQ=255)                                                                           aTCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                         >  2:22827/1‑151 (MQ=255)                                                                            tCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc                                                                        >  2:80966/1‑151 (MQ=255)                                                                               ggCTTGCTCCTTAAAAGTTAACAAAC‑CCGCCGGCAATATAAAGGCAGGGCGCTTTGCTGTCCATCGTTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                         <  1:413973/147‑1 (MQ=255)                                                                                gggtgtggctTCAAAGTAAAAAAAC‑CCGCGGGGGATATAAGGGCAGGCCTTTTTGCTCTCCTCCATTCCCCCCGC‑GGGATTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                         <  1:108439/139‑1 (MQ=255)                                                                                gCGTCCTGCTTAAAATTTAAAAAAC‑CCGCCGCCAATAAAAAGCAGGGGCGACTCGCTTCCCATCATTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGa                                                                      <  2:958727/149‑1 (MQ=255)                                                                                 cGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGatat                                                                   >  2:839322/1‑151 (MQ=255)                                                                                    gctgctTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc                                                                >  1:1022049/1‑151 (MQ=255)                                                                                         ttaaaattaaaaaaaa‑CCGCGGGCAAAATCAGGGCAGGGCTCTTTGCTGCCCATCATTCCCCCCGG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCa                                                               <  2:147414/135‑1 (MQ=255)                                                                                            aaatttaaaaaac‑CCGCCGGCGAAACAAAGGCGTGGCGATTTCTTGTCCATCTGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg                                                              <  2:78379/136‑1 (MQ=255)                                                                                            aaaattacaaaaccccGGCGGCGATAACAAGGGAGGGCGTCTTTCTGCCCGTCAGTCCCCCCAG‑GGAAATCGCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGata                                                                    <  1:1091994/126‑1 (MQ=255)                                                                                             aaGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGCGGATCAGGCCGTCGATATTCCAGGAGAGc                                                        >  2:703419/1‑150 (MQ=255)                                                                                                  aaaaaac‑CCGACGGCAATATAAGGGAATGGCGACTTGCTGCCCATCAGTCCCCCCGC‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt                                                      <  2:631884/144‑1 (MQ=255)                                                                                                  aTCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAt                                                  >  1:507131/1‑151 (MQ=255)                                                                                                    aaaac‑CCGACGGCGATAACAGGGCTGGGCGTTTTGCTGCCCATCAGTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTc                                                <  2:905396/150‑1 (MQ=255)                                                                                                      aaa‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCg                                               >  2:816753/1‑150 (MQ=255)                                                                                                       aa‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCg                                               >  1:862571/1‑149 (MQ=255)                                                                                                        acccGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCAGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAg                                             <  2:837929/149‑1 (MQ=255)                                                                                                            gACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGt                                         >  1:968287/1‑151 (MQ=255)                                                                                                            gACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGAGCGCGGAGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCTGGAGAGCATGGATTCGAGGCg                                          >  1:162389/1‑150 (MQ=255)                                                                                                               ggCGATACCAGGGGATGGCGCTTTGCGCCCCATCAGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGtt                                        <  2:1232005/149‑1 (MQ=255)                                                                                                               ggCGATACCAAGGCAGGGCGTTTTGCTGCCCATCATTCCCCCCGG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAgg                                            <  2:1142330/145‑1 (MQ=255)                                                                                                               ggCGATACAAGGGCGTGGCGCCTTGCTGCCCATCAGTCCCCCCGC‑GGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGtt                                        <  1:1236489/149‑1 (MQ=255)                                                                                                               ggCGATAACAAGGCAGGGCGATTTGCTCCCCATCATTCCCCCCAG‑GGAAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTc                                       <  1:561381/150‑1 (MQ=255)                                                                                                               ggCGAAATCAAGCCAGGCCGCCTTGCTGCCCATCATTTCCCCCAG‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTc                                       <  2:1213857/150‑1 (MQ=255)                                                                                                                  gATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc                                   >  2:488935/1‑151 (MQ=255)                                                                                                                  gATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc                                   >  1:1222990/1‑151 (MQ=255)                                                                                                                        aaGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAtt                             >  1:174393/1‑151 (MQ=255)                                                                                                                        aaGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAtt                             >  2:353968/1‑151 (MQ=255)                                                                                                                          ggcgtggcgtTTTGCTCTCAATCATTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAt                              <  1:1247168/148‑1 (MQ=255)                                                                                                                          ggaagggcgctttGCTGTCCATTATTTCCCCCGC‑GGAAAACCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGtt                                        <  1:650951/127‑1 (MQ=255)                                                                                                                                  gACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTtat                   >  2:86263/1‑151 (MQ=255)                                                                                                                                  gACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTtat                   >  2:961136/1‑151 (MQ=255)                                                                                                                                        cTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCg              >  2:251986/1‑150 (MQ=255)                                                                                                                                           tCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGCTATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGt             >  1:733488/1‑148 (MQ=255)                                                                                                                                           tCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTa            >  1:86514/1‑149 (MQ=255)                                                                                                                                               attggtCCACCACGGGGACTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAg           <  1:209028/142‑1 (MQ=255)                                                                                                                                                   ttCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGc  >  2:443400/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                    TGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGC  >  NC_000913/3578141‑3578436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑