Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,578,301	0	A	C	41.8%	98.4 / 20.8	67	G142G (GGT→GGG)	gntR	d‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (30/9);  new base C (0/28);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 2.44e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCG  >  NC_000913/3578153‑3578451                                                                                                                                                       |                                                                                                                                                       cgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATAc                                                                                                                                                       <  2:1045143/150‑1 (MQ=255)  gtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATAccc                                                                                                                                                     >  1:1133565/1‑151 (MQ=255)    cgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCg                                                                                                                                                    <  1:1027704/150‑1 (MQ=255)    cgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGc                                                                                                                                                   <  1:419186/151‑1 (MQ=255)       gaCGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGGGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATCCCCGCCa                                                                                                                                                 <  1:605898/150‑1 (MQ=255)          gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTc                                                                                                                                             >  2:375587/1‑151 (MQ=255)          gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTCCCCCCAC‑GGGAATACCCGCCACtt                                                                                                                                              <  1:239497/150‑1 (MQ=255)               ccGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAatc                                                                                                                                         >  2:1237718/1‑150 (MQ=255)                     tAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCttt                                                                                                                                   >  1:845789/1‑150 (MQ=255)                         cAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAgg                                                                                                                               >  1:100560/1‑150 (MQ=255)                         aattgtgGCGATCCCCGCGAGAAAAAATGGCAGTGGTCATCGGGGGTGCTCCTTAAAATTTAAAAAAC‑CCGACGGCAATATCAGGGCAGGGCGCTTTGTTGTCCATCAGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGt                                                                                                                              <  2:790383/148‑1 (MQ=255)                             ggggCGATGCCCGCGAGAAATAATGGCAGTGGTCATCTGGCGTGCTGCTTAAAAGTTAAAAAAC‑CCGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCATTCCCCCCAG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTg                                                                                                                             <  2:965035/146‑1 (MQ=255)                             ggggCGATCCCCGCGAGAAATAGGGGGAGTGTTCTTCTGGCGTGCTGCTAAAAATTTATAAAAC‑CCGGCGGCGATACAAGGGCAGGGCGACTTGCTGCCCACCATTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAgg                                                                                                                               <  2:1096245/144‑1 (MQ=255)                               ggCGTTCCCAGCGAGAAATTGTGGACGTGGTCAGCGGTCGTGCTGATTAAAATTTTTCAAAT‑CCGAGGGCGATACAATGGCAGGGCGCCTTGCTGTCAATCGTTTCCCCCAC‑GGAATTCCCCGCCACTTCAATCATCTTTAAGGTGcgcg                                                                                                                         <  2:1113855/150‑1 (MQ=255)                                gCGATCCCCGCGAACAATAAGGGCGGTGGTCTTCTGGCGTGCTGCTTAAATTTAATCAAAC‑CCGCCGGCAATATCAAGGCATGGCGTTTTGCTGTCCATCAGTCCCCCCCC‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGcgcg                                                                                                                         <  2:794970/149‑1 (MQ=255)                                    tGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtg                                                                                                                    >  2:60470/1‑150 (MQ=255)                                     gCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCgcggc                                                                                                                       >  1:209465/1‑146 (MQ=255)                                      cccGCGAAAAATAGGGGCGGGGGTCATCTGGCGTCCTGCTTAAAAGTTAAAAAAA‑CCGACGGCAATAACAAGGCATGGCAACTTGCTCTACATCGGTCCCCCCAG‑GGTAATCCCCGCCACTTCAATCATCTTTAAGGTGcgcg                                                                                                                         <  1:350865/143‑1 (MQ=255)                                       ccGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTggg                                                                                                                >  1:196641/1‑151 (MQ=255)                                                     ggCGGTGGTCATGTGGCGTCCTGCTCAAAAGTTCACAACC‑CCCCCGGAGATACGCGGGGAGGGCGTCTTGCTGCCCACCATTCCCCCCAG‑GGGAATAGCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa                                                                                                    <  1:36835/149‑1 (MQ=255)                                                     ggCGGTGGTCACCGGGCGTGCTCCTAAAAATTTAAAAAAC‑CCGACGGCATAATCAGGGCTGGGCCTCTTGTTGCCCATCTGTCCCCCCGG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa                                                                                                    <  1:1245820/149‑1 (MQ=255)                                                     gcgagcatgcATCTGGCGTTCT‑CTTCAAAGTTAAAAAAACCCGCGGGCGATATAAAGCCAGGGGCGTTTTCTACCCGCCGGTCCCCCCCC‑GGAAAGACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAgg                                                                                                  <  2:978123/142‑1 (MQ=255)                                                           ggTCGTCGGGCGGGCTGATTAAAATTAAAAAAAA‑CCGGGGAATATATAAAGGCATGCCAGTTTGCTGTCCATCAGTCCCCCGAA‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAg                                                                                             <  1:677084/150‑1 (MQ=255)                                                              cATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCg                                                                                         >  1:1088387/1‑151 (MQ=255)                                                              cATCGGGGGTGCTGCTAAAAAGTTAAAAAAC‑CCGACGGCTATATCAAGGCATGGCGTTTTGCTGCCCATCAGTCCCCCCAG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc                                                                                          <  1:432558/150‑1 (MQ=255)                                                              cATCGGGCGTGCGGCTTAAAATTTATAAAAC‑CCCCGGGCAAAATAAGGGCCGGCGGTCTTGCTGCCCGTAATTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc                                                                                          <  1:473568/150‑1 (MQ=255)                                                               aTCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                                        >  1:35123/1‑151 (MQ=255)                                                               aTCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                                        >  2:22827/1‑151 (MQ=255)                                                                tCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc                                                                                       >  2:80966/1‑151 (MQ=255)                                                                   ggCTTGCTCCTTAAAAGTTAACAAAC‑CCGCCGGCAATATAAAGGCAGGGCGCTTTGCTGTCCATCGTTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                                        <  1:413973/147‑1 (MQ=255)                                                                    gggtgtggctTCAAAGTAAAAAAAC‑CCGCGGGGGATATAAGGGCAGGCCTTTTTGCTCTCCTCCATTCCCCCCGC‑GGGATTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt                                                                                        <  1:108439/139‑1 (MQ=255)                                                                    gCGTCCTGCTTAAAATTTAAAAAAC‑CCGCCGCCAATAAAAAGCAGGGGCGACTCGCTTCCCATCATTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGa                                                                                     <  2:958727/149‑1 (MQ=255)                                                                     cGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGatat                                                                                  >  2:839322/1‑151 (MQ=255)                                                                        gctgctTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc                                                                               >  1:1022049/1‑151 (MQ=255)                                                                             ttaaaattaaaaaaaa‑CCGCGGGCAAAATCAGGGCAGGGCTCTTTGCTGCCCATCATTCCCCCCGG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCa                                                                              <  2:147414/135‑1 (MQ=255)                                                                                aaatttaaaaaac‑CCGCCGGCGAAACAAAGGCGTGGCGATTTCTTGTCCATCTGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg                                                                             <  2:78379/136‑1 (MQ=255)                                                                                aaaattacaaaaccccGGCGGCGATAACAAGGGAGGGCGTCTTTCTGCCCGTCAGTCCCCCCAG‑GGAAATCGCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGata                                                                                   <  1:1091994/126‑1 (MQ=255)                                                                                 aaGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGCGGATCAGGCCGTCGATATTCCAGGAGAGc                                                                       >  2:703419/1‑150 (MQ=255)                                                                                      aaaaaac‑CCGACGGCAATATAAGGGAATGGCGACTTGCTGCCCATCAGTCCCCCCGC‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt                                                                     <  2:631884/144‑1 (MQ=255)                                                                                      aTCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAt                                                                 >  1:507131/1‑151 (MQ=255)                                                                                        aaaac‑CCGACGGCGATAACAGGGCTGGGCGTTTTGCTGCCCATCAGTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTc                                                               <  2:905396/150‑1 (MQ=255)                                                                                          aaa‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCg                                                              >  2:816753/1‑150 (MQ=255)                                                                                           aa‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCg                                                              >  1:862571/1‑149 (MQ=255)                                                                                            acccGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCAGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAg                                                            <  2:837929/149‑1 (MQ=255)                                                                                                gACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGt                                                        >  1:968287/1‑151 (MQ=255)                                                                                                gACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGAGCGCGGAGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCTGGAGAGCATGGATTCGAGGCg                                                         >  1:162389/1‑150 (MQ=255)                                                                                                   ggCGATACCAGGGGATGGCGCTTTGCGCCCCATCAGTCCCCCCGG‑GGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGtt                                                       <  2:1232005/149‑1 (MQ=255)                                                                                                   ggCGATACCAAGGCAGGGCGTTTTGCTGCCCATCATTCCCCCCGG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAgg                                                           <  2:1142330/145‑1 (MQ=255)                                                                                                   ggCGATACAAGGGCGTGGCGCCTTGCTGCCCATCAGTCCCCCCGC‑GGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGtt                                                       <  1:1236489/149‑1 (MQ=255)                                                                                                   ggCGATAACAAGGCAGGGCGATTTGCTCCCCATCATTCCCCCCAG‑GGAAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTc                                                      <  1:561381/150‑1 (MQ=255)                                                                                                   ggCGAAATCAAGCCAGGCCGCCTTGCTGCCCATCATTTCCCCCAG‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTc                                                      <  2:1213857/150‑1 (MQ=255)                                                                                                      gATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc                                                  >  1:1222990/1‑151 (MQ=255)                                                                                                      gATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc                                                  >  2:488935/1‑151 (MQ=255)                                                                                                            aaGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAtt                                            >  1:174393/1‑151 (MQ=255)                                                                                                            aaGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAtt                                            >  2:353968/1‑151 (MQ=255)                                                                                                              ggcgtggcgtTTTGCTCTCAATCATTCCCCCCAG‑GGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAt                                             <  1:1247168/148‑1 (MQ=255)                                                                                                              ggaagggcgctttGCTGTCCATTATTTCCCCCGC‑GGAAAACCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGtt                                                       <  1:650951/127‑1 (MQ=255)                                                                                                                      gACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTtat                                  >  2:86263/1‑151 (MQ=255)                                                                                                                      gACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTtat                                  >  2:961136/1‑151 (MQ=255)                                                                                                                            cTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCg                             >  2:251986/1‑150 (MQ=255)                                                                                                                               tCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGCTATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGt                            >  1:733488/1‑148 (MQ=255)                                                                                                                               tCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTa                           >  1:86514/1‑149 (MQ=255)                                                                                                                                   attggtCCACCACGGGGACTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAg                          <  1:209028/142‑1 (MQ=255)                                                                                                                                       ttCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGc                 >  2:443400/1‑151 (MQ=255)                                                                                                                                        ccccccac‑GGAATTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGc                 <  1:696233/146‑1 (MQ=255)                                                                                                                                            ccag‑GGGAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCAt               <  2:22582/148‑1 (MQ=255)                                                                                                                                                 ggaaaTACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCt          <  2:378686/146‑1 (MQ=255)                                                                                                                                                       aCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAAcc   >  2:1053003/1‑150 (MQ=255)                                                                                                                                                       aCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCg  >  1:1239038/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                       CGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCAC‑GGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCG  >  NC_000913/3578153‑3578451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑