Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,640,9810CT43.4% 51.5 / 36.3 54F40F (TTC→TTTdtpBdipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (3/27);  new base T (23/0);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 4.17e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGT  >  NC_000913/3640832‑3641130
                                                                                                                                                       |                                                                                                                                                          
tttCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc                                                                                                                                                            <  1:979360/150‑1 (MQ=255)
 ttCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttct                                                                                                                                                           <  2:963930/150‑1 (MQ=255)
       aTGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTaa                                                                                                                                                  <  2:455792/151‑1 (MQ=255)
            aTTTGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTaa                                                                                                                                                  >  2:123155/1‑146 (MQ=255)
            aTTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTCTTTCTTTAACaa                                                                                                                                              >  2:938614/1‑149 (MQ=255)
               ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTGTTCTGTCAAACattt                                                                                                                                            >  1:593043/1‑147 (MQ=255)
               ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCtt                                                                                                                                          <  2:575775/150‑1 (MQ=255)
                 aaGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCTTTAAACAACTTgg                                                                                                                                        >  2:352012/1‑150 (MQ=255)
                    gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTCCttta                                                                                                                                                  >  2:356191/1‑136 (MQ=255)
                     ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAGCTTGGGtttt                                                                                                                                   >  2:534260/1‑149 (MQ=255)
                      taataaATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTtaaaaacctggggttt                                                                                                                                    >  2:456517/1‑137 (MQ=255)
                       aataaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTCTTTaaaagcgtgggttttc                                                                                                                                    >  2:49252/1‑137 (MQ=255)
                        ataaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGc                                                                                                                                <  1:640983/151‑1 (MQ=255)
                              atGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTAAAAGCTTGGGTTCTCGCAagag                                                                                                                          >  2:249684/1‑150 (MQ=255)
                                    acaacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGCTTTGGGTCTCCCCAAAGCAGGCtt                                                                                                                   >  2:1040258/1‑151 (MQ=255)
                                     caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCttt                                                                                                                  <  1:630400/151‑1 (MQ=255)
                                     caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCttt                                                                                                                  <  1:994127/151‑1 (MQ=255)
                                       acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTTTTTAAAAGCCTGGGTTTTCGCTACAGCAgggctt                                                                                                                  >  1:589850/1‑145 (MQ=255)
                                        caacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGt                                                                                                               <  2:1204777/151‑1 (MQ=255)
                                         aacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTc                                                                                                              <  1:291300/151‑1 (MQ=255)
                                             cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACtt                                                                                                          <  2:452567/151‑1 (MQ=255)
                                              ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGCTTTGGTTTTTGCCAAAGCAGGGCTTTTTTACttt                                                                                                         >  2:132552/1‑151 (MQ=255)
                                                  ggggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTgg                                                                                                      <  1:702314/150‑1 (MQ=255)
                                                     gATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCt                                                                                                  <  1:570485/151‑1 (MQ=255)
                                                      aTGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCtt                                                                                                 <  2:770500/151‑1 (MQ=255)
                                                      aTGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGCTTTTTTCGTTTAAAAACCTTGGGTTTCGCCAGAGCAGGCCTTTTTTTCtttttggg                                                                                                    >  2:613103/1‑144 (MQ=255)
                                                        gctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTAACTTTTGGTGCttt                                                                                                <  2:757645/150‑1 (MQ=255)
                                                             agcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAACGTGGAGTCTCTCAAGAGCAGGGTGTTTTTACTTTTTGGGCtttt                                                                                               >  2:351004/1‑146 (MQ=255)
                                                             agcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTtgctgc                                                                                          <  2:729612/151‑1 (MQ=255)
                                                                 aCCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTg                                                                                      <  1:697971/151‑1 (MQ=255)
                                                                 aCCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTg                                                                                      <  1:452813/151‑1 (MQ=255)
                                                                     cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCt                                                                                  <  1:127085/151‑1 (MQ=255)
                                                                       cccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTCTTTAAAAACCTGGGGTTTCGCCAAAGCGGGCGTTTTTTTATTTTTGTGCCTTTGTTTCGCTgggcgtt                                                                                >  1:512240/1‑146 (MQ=255)
                                                                        ccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATg                                                                              <  2:937115/151‑1 (MQ=255)
                                                                              ttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAACTTTGGTTTTCCCCAGAGGAAGGCTTTTTTACTTTTTGTGGTTTTTCTTCGCCGGGCttttggc                                                                            >  2:905591/1‑142 (MQ=255)
                                                                               tCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAACTTTGATTTTTGCAAAAGCAGGCCTTTTTTACTTTTTGTGCTTTTTCTTTGCTGGGCTATGGcc                                                                           >  1:497879/1‑147 (MQ=255)
                                                                               tCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCa                                                                        <  1:148778/150‑1 (MQ=255)
                                                                                cATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGCTTTTTTTGTTTAACAGGTTGGGTTTTTGCTAGAGCAGGGTTTTGTCCCTTTTTGGGCGTTTTCTGCGCCGGGCTATGGCCTCCtt                                                                      >  2:243554/1‑151 (MQ=255)
                                                                                  tGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAGATTTGGTTTTTGGAAAAGGAAGGCTTTTGTCCTTTTTGGGGTTTTTTTTGCGCGGGCTTATGGCTCAttt                                                                     >  2:696839/1‑150 (MQ=255)
                                                                                       ttttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAGCTTTGTTTTTCGCGAGAGCAGGGGTTTTTCCTTTTTTTTGGTTTTTGTGCGCCGGGCTTTTGGCTCCttt                                                                     >  1:425719/1‑145 (MQ=255)
                                                                                         ttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTCTTTGTTTAAAAACTTTGGTTCTCCCAAAAGCAGGGCTTTTTCACTTTTTGTGCTTTTTTTTCGCTGGGCTATTGGCCCCTTTTcctt                                                                 >  1:1110252/1‑146 (MQ=255)
                                                                                           ttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTCTTTGTTTAAAAACTTGGGTTTTCGCCAGAGCAGGCCTTTTTCACTTTTTGTGCTTTTTCTGTGCTCGGCTTTGGCCTCCTTTCCTTTtgcgg                                                           >  2:61214/1‑151 (MQ=255)
                                                                                            tGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAAAAGCTTTGGTTCTCGCAAGAGCAGGGTTTTTTCACTTTTTGTGCTTTTTCTTCGCTGGTCTATTGCCTCATTTTCATTggcgg                                                           >  2:854017/1‑150 (MQ=255)
                                                                                              tCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCt                                                         <  1:561531/150‑1 (MQ=255)
                                                                                               cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAACTTGGGTTTTCGCAAAAGCAAGCTTTTTTTACTTTTTGTGGTTTTTTTTCGCTGGGCTATGGGCCCAttttcctttgggg                                                           >  2:1017369/1‑137 (MQ=255)
                                                                                               cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGCCTGGGTTTTCGCCAGAACAGGCTTTTTTCCCTTTTTGTGCTTTTTCTGCGCTGGGCTTTGGCCTCCttttcctttg                                                              >  2:429455/1‑137 (MQ=255)
                                                                                               cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTAt                                                       <  2:725023/151‑1 (MQ=255)
                                                                                                gAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCTTTTAACAGCTTGGGTTTTCGCCAGAGCAGGCTTTTTTTACCTTTTGTGGTTTTGCTGGGGTGGTGTATTGGCCTCtttttttttgcgg                                                            >  2:492145/1‑137 (MQ=255)
                                                                                                    tATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTCTTAAAAAACTTGGGTTTTCGCCAGAGCAGGGGTTTTTCCCTTTTTGTGCTTTTTCTGCGCTGGTGTTTTGCCTCATTTTCAtttgggggctt                                                       >  2:214309/1‑137 (MQ=255)
                                                                                                     aTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCgg                                                  <  2:1226667/150‑1 (MQ=255)
                                                                                                         gAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGacc                                             <  2:172876/151‑1 (MQ=255)
                                                                                                                   gCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTggg                                   <  2:262639/151‑1 (MQ=255)
                                                                                                                         acGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGAcc                               <  1:143856/149‑1 (MQ=255)
                                                                                                                          cGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCaa                             <  1:104003/150‑1 (MQ=255)
                                                                                                                                   gggCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCAtt                   <  2:845583/151‑1 (MQ=255)
                                                                                                                                            ggCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTgg           <  2:379325/150‑1 (MQ=255)
                                                                                                                                                     ttcttcGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACtt    >  1:807882/1‑150 (MQ=255)
                                                                                                                                                      tcttcGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTtg   <  1:475868/150‑1 (MQ=255)
                                                                                                                                                       cttcGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTtgt  >  1:996073/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                          
TTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGT  >  NC_000913/3640832‑3641130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: