| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,875 | 0 | A | T | 41.7% | 28.0 / 11.4 | 24 | N272K (AAT→AAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base A (12/2); new base T (0/10); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCC > NC_000913/357729‑358007 | aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTAtttt < 2:721118/151‑1 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑ccgc > 2:705651/1‑151 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑ccgccg > 2:66617/1‑151 (MQ=255) aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGt > 2:283903/1‑151 (MQ=255) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTcc > 1:591759/1‑151 (MQ=255) ttccgcccGCAGCCTTGTGTCGGGGGCTCCCTTTATTTTTTCCCCCAAACACCCGCGATTTTTCCCACCCCCTTTTCCAAAATTC‑CCTCCGGGGGGCCTGTCTCCCATTTTTTTTC‑CCGCCCCAAAAAAACCGCCGTTCTCTCCagtag < 1:686651/143‑1 (MQ=255) ccGCAGTCCTGTGTCAGGGTCTCCCGTGTTTCATTCCCGCCAACACCCGCGCTTTTACCCACCGCCATGTCAAAAAATC‑CCTTCGGGGGGGTTGTCTGCCAGTTTTTTTC‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg < 1:484705/149‑1 (MQ=255) ccGCAGCCTTGGGTCGGCGCTTCCGGTAATTCATTCCCCCCAACACCCGCCCTTTTACCCACCCCCTTGTAAAAAAATC‑CCTGGGCGGGGGCTTTCGCCCGGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCg < 2:180300/148‑1 (MQ=255) ccTGTGTCGGCGGCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCATTTACCCACCGCCAGGTGAAAAAATG‑CTTTCGCGGCGGCTGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAgag‑a < 1:561047/151‑1 (MQ=255) ccTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:766202/1‑144 (MQ=255) tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggg > 2:252880/1‑142 (MQ=255) gcgcctcccggatttCATTCCCCCCAACACCCCCGCTTTTACCCACCCCATTGAGAAAAAATG‑CCTCCGGGGCGGCTGCCGGCCAGCTATTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAAt < 2:82688/132‑1 (MQ=255) cGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAG‑aaaaaaaatt > 1:795047/1‑143 (MQ=255) cggtgtTTCTTTCCCGCCAACACCCCCGCTTTTACCCACCCCCTTGTGAAAAAATC‑CCTCCGGGGCGGTGTTCTGCAAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTaa < 2:537498/144‑1 (MQ=255) ttattcattcCCGCAAACACCCGCGCTTTTACCCAACGCCATGTGAAAAAATC‑CCTTCGCGGCGGCTGTCTGCCAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGcc < 1:461445/144‑1 (MQ=255) gATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGCGAG‑AAAAAAC‑ttttaaagccctct > 1:167716/1‑140 (MQ=25) aacaccCGCGTTTTTACCCAACCCCTTGTAAAAAAATC‑CCTCCGCGGGGGTTGTCTCCCAGTTTTTTTC‑CCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATc < 1:401865/151‑1 (MQ=255) aacaccCGCGCTTTTACCCACCGCCTGGTG‑AAAAATGCCCTCCGCGGCGCTTGTCGCCCGGTTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATc < 2:699383/151‑1 (MQ=255) tttACCCACCCCCATGTAAAAAAAGC‑CCTCCGCGGGGGCTGTCTCCAAGTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:183113/149‑1 (MQ=255) ttATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAG‑AAAAAAA‑TTTTAACCCCTCATGTTTTTTGGGGAACGCTGCTggg > 1:612308/1‑146 (MQ=25) gaaaaaaaGC‑CCTCCGGGGGGGCTGTC‑GCCAATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTgg < 2:723017/139‑1 (MQ=255) aaaaTG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAAAC‑TTTAACCCCGCCTTGTT‑TGTGGGGAATGCTGGTGGGTACAAATTGGGAAggggaagggg > 2:780439/1‑144 (MQ=255) tG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAAG‑AAAAAAA‑TTTTAACCCCGCACGTTTTGTGGGGATAGCTGCTGGGGACAAAGTGGGgaaggaagggggggg > 1:314234/1‑143 (MQ=255) ggcTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGAG‑AAAAAACATTTAATCCC‑CCCTGTTTGGTGGGAATCCCTGCTGGGGAACACGTGGGGAGGGGAGGgcggcgggacaagccc > 2:404177/1‑138 (MQ=255) | AACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCC > NC_000913/357729‑358007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |