| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 54.2% | 6.9 / 36.7 | 24 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (10/1); new base A (0/13); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.61e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-03 | |||||||||||
AGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTG > NC_000913/357751‑358023 | aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGt > 2:283903/1‑151 (MQ=255) cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTcc > 1:591759/1‑151 (MQ=255) ttccgcccGCAGCCTTGTGTCGGGGGCTCCCTTTATTTTTTCCCCCAAACACCCGCGATTTTTCCCACCCCCTTTTCCAAAATTC‑CCTCCGGGGGGCCTGTCTCCCATTTTTTTTC‑CCGCCCCAAAAAAA‑CCGCCGTTCTCTCCagtag < 1:686651/143‑1 (MQ=255) ccGCAGTCCTGTGTCAGGGTCTCCCGTGTTTCATTCCCGCCAACACCCGCGCTTTTACCCACCGCCATGTCAAAAAATC‑CCTTCGGGGGGGTTGTCTGCCAGTTTTTTTC‑CCGCCGAAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgg < 1:484705/149‑1 (MQ=255) ccGCAGCCTTGGGTCGGCGCTTCCGGTAATTCATTCCCCCCAACACCCGCCCTTTTACCCACCCCCTTGTAAAAAAATC‑CCTGGGCGGGGGCTTTCGCCCGGTTTTTTTC‑CCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCg < 2:180300/148‑1 (MQ=255) ccTGTGTCGGCGGCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCATTTACCCACCGCCAGGTGAAAAAATG‑CTTTCGCGGCGGCTGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAgag‑a < 1:561047/151‑1 (MQ=255) ccTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggg > 1:766202/1‑144 (MQ=255) tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCgggggg > 2:252880/1‑142 (MQ=255) gcgcctcccggatttCATTCCCCCCAACACCCCCGCTTTTACCCACCCCATTGAGAAAAAATG‑CCTCCGGGGCGGCTGCCGGCCAGCTATTTTC‑CCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAAt < 2:82688/132‑1 (MQ=255) cGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAG‑aaaaaaaatt > 1:795047/1‑143 (MQ=255) cggtgtTTCTTTCCCGCCAACACCCCCGCTTTTACCCACCCCCTTGTGAAAAAATC‑CCTCCGGGGCGGTGTTCTGCAAGTTTTTTTC‑CCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTaa < 2:537498/144‑1 (MQ=255) ttattcattcCCGCAAACACCCGCGCTTTTACCCAACGCCATGTGAAAAAATC‑CCTTCGCGGCGGCTGTCTGCCAGCTTTTTTC‑CCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGcc < 1:461445/144‑1 (MQ=255) gATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCGCGAG‑AAAAAAC‑ttttaaagccctct > 1:167716/1‑140 (MQ=25) aacaccCGCGTTTTTACCCAACCCCTTGTAAAAAAATC‑CCTCCGCGGGGGTTGTCTCCCAGTTTTTTTC‑CCGCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATc < 1:401865/151‑1 (MQ=255) aacaccCGCGCTTTTACCCACCGCCTGGTG‑AAAAATGCCCTCCGCGGCGCTTGTCGCCCGGTTTTTTTC‑CCCCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATc < 2:699383/151‑1 (MQ=255) tttACCCACCCCCATGTAAAAAAAGC‑CCTCCGCGGGGGCTGTCTCCAAGTTTTTTTC‑CCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:183113/149‑1 (MQ=255) ttATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAG‑AAAAAAA‑TTTTAACCCCTCATGTTTTTTGGGGAACGCTGCTggg > 1:612308/1‑146 (MQ=25) gaaaaaaaGC‑CCTCCGGGGGGGCTGTC‑GCCAATTTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTgg < 2:723017/139‑1 (MQ=255) aaaaTG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAAAC‑TTTAACCCCGCCTTGTT‑TGTGGGGAATGCTGGTGGGTACAAATTGGGAAggggaagggg > 2:780439/1‑144 (MQ=255) tG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAAG‑AAAAAAA‑TTTTAACCCCGCACGTTTTGTGGGGATAGCTGCTGGGGACAAAGTGGGgaaggaagggggggg > 1:314234/1‑143 (MQ=255) ggcTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGAG‑AAAAAACATTTAATCCC‑CCCTGTTTGGTGGGAATCCCTGCTGGGGAACACGTGGGGAGGGGAGGgcggcgggacaagccc > 2:404177/1‑138 (MQ=255) ccattttttttc‑CCCCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa < 1:317665/144‑1 (MQ=255) ttttttc‑CCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg < 2:372799/148‑1 (MQ=255) tttttt‑CCCCCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt < 2:53353/147‑1 (MQ=255) | AGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTG > NC_000913/357751‑358023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |