Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	501,564	0	A	C	51.1%	56.5 / 84.6	47	*559E (TAA→GAA)	ybaL	inner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base A (20/3);  new base C (0/24);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.81e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-09

TACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCA  >  NC_000913/501415‑501712                                                                                                                                                       |                                                                                                                                                     tACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTa                                                                                                                                                      <  2:766969/150‑1 (MQ=255)    tGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcccc                                                                                                                                                  >  2:9179/1‑151 (MQ=255)             tAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCaccacc                                                                                                                                         >  2:567723/1‑151 (MQ=255)                    tcgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccg                                                                                                                                  >  1:695720/1‑151 (MQ=255)                     cgtcgtTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccg                                                                                                                                  <  2:706637/150‑1 (MQ=255)                     cgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccg                                                                                                                                  <  2:492408/150‑1 (MQ=255)                          gTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTg                                                                                                                            >  1:363941/1‑151 (MQ=255)                              gTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGt                                                                                                                        >  2:692624/1‑151 (MQ=255)                                 ggCCAGAAAGGGCGTTCACGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTAATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGt                                                                                                                        <  1:784839/148‑1 (MQ=37)                                     aGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                                                                                                 >  1:336927/1‑151 (MQ=255)                                        tAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGt                                                                                                              >  1:175514/1‑151 (MQ=255)                                               tccACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCCCCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAg                                                                                                         <  1:692935/147‑1 (MQ=39)                                                 cccgccgcATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TTTTATCATCCCTACATATTTTTCATATTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                                                                                       <  1:616784/147‑1 (MQ=38)                                                   cgccgcATTTGGCTTTTGGCTCTCGATGCCTGAGGCGCCGCTGGCGCG‑TTTTATCATGCCTACATATTTTTCATATTTTCCACCCGGCAACCACCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt                                                                                                   <  1:390653/151‑1 (MQ=255)                                                             ggCTTTTGGCTCTCGATCCCTGATGCGCCGCGGGCGCG‑TCTTATCATGCCTACATATTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg                                                                                              <  1:378340/146‑1 (MQ=255)                                                             ggCTTTTGCCTCTCGATGCCGGATGCGCCGCGGGCGCT‑TTTTATCTTGCCAACATTTTTTTCATTTTTTACACCCGGCAACCCCCGTTTCCCCCCTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt                                                                                          <  1:208619/150‑1 (MQ=255)                                                              gcttttGGCTCTCGATCCCTGATGCGCCGCGGGCGCT‑TCTTATCATGCCTACATTTTTTTCATTTTTTCCACCGGGCACCCCCCTTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc                                                                                             <  2:28182/143‑1 (MQ=255)                                                              gCATTGGGCTCTCGTTGCTGGTGGCGACGCGGGCGCT‑TTTTATCATGCCTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt                                                                                          <  2:378040/149‑1 (MQ=255)                                                                 tttGGCTCTCGATGCTTGATGCGCCGCGGGCGCT‑TTTTATCATGCCTACATATTTTTCATTTTTTCCACCCGGCACCCACCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt                                                                                        <  2:400132/148‑1 (MQ=255)                                                                      ctttcGTTGCTTGATGGGGCGCTGCCGTG‑TTTTCTCTTCCCTCAATTTTTTTCATTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTc                                                                                 <  2:455183/147‑1 (MQ=255)                                                                        ctcGATGCTTGTTGCGACGC‑GGGGCGTTTTTTTCATCCCTCATTTTTTTTCATTTTTAACACCCGGCCCCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCg                                                                                <  2:451534/149‑1 (MQ=255)                                                                         tcGATGCTTGAGGCGCCGCTGGCGCG‑TTTTATCATCCCTACTTTTTTTTCATTTTTTCCATCCGCCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTc                                                                                 <  2:225383/147‑1 (MQ=255)                                                                               ccTGAGGCGACGCTGGCGCT‑TTTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTc                                                                                 <  1:629137/141‑1 (MQ=255)                                                                                   aTGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCt                                                                   >  2:510262/1‑151 (MQ=255)                                                                                         cgcgGGGGCG‑TCTTTTCTTGCCTATTTTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAt                                                                <  2:283554/148‑1 (MQ=255)                                                                                         cgcgGGCGCT‑TCTTATCATGCCTACATTTTTTCCATATTTTACACCCGGCACCCCCCGTTTACCCCCTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt                                                               <  2:363134/149‑1 (MQ=255)                                                                                          gCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGc                                                            >  1:609323/1‑151 (MQ=255)                                                                                               cgcg‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcaccac                                                       >  1:171691/1‑151 (MQ=255)                                                                                                 cg‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGt                                                     >  2:714597/1‑151 (MQ=255)                                                                                                      ttATCATGCCTACATATTTTTCATATTTTACCTCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                                 >  1:470079/1‑151 (MQ=255)                                                                                                      ttATCATGCCTACATATTTTTAATATTTTACACCCGGCACCCCCCTTTTCCCCCGCCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                                    <  1:9179/148‑1 (MQ=255)                                                                                                        aTCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                               >  2:371879/1‑151 (MQ=255)                                                                                                           aTGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGat                                             >  1:107984/1‑150 (MQ=255)                                                                                                                 actttttttaaattttttACATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc                                      <  1:184805/137‑1 (MQ=255)                                                                                                                    tatTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCAc                                    >  1:275438/1‑150 (MQ=255)                                                                                                                      tttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                                 >  1:637623/1‑151 (MQ=255)                                                                                                                      tttttCATATTTTACACCCGCCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACtt                                  <  2:231683/150‑1 (MQ=255)                                                                                                                        tttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCAt                               >  1:141603/1‑151 (MQ=255)                                                                                                                            attttttACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCAt                               <  1:631549/144‑1 (MQ=255)                                                                                                                             tatTTTCCATCCGGCACCCCCCTTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATc                              <  1:84365/147‑1 (MQ=255)                                                                                                                                tttACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATa                        >  1:53108/1‑150 (MQ=255)                                                                                                                                   aCATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATgg                    >  1:699264/1‑151 (MQ=255)                                                                                                                                    cacccgcaaccccccGTTTCCCCCGTCCCCCCCCCGCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg                     <  1:652873/136‑1 (MQ=255)                                                                                                                                    cacccGGCAACCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATgg                    <  1:496464/147‑1 (MQ=255)                                                                                                                                    cacccGCCACCCCCCTTTTCCCCCGTCCCCACCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAAt                      <  1:618497/145‑1 (MQ=255)                                                                                                                                            aaCCACCTTTTCCCCCTCCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGc            <  1:741490/150‑1 (MQ=255)                                                                                                                                                    tttCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtc     <  2:380257/149‑1 (MQ=255)                                                                                                                                                      tACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCa  >  2:8291/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     TACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCG‑TCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCA  >  NC_000913/501415‑501712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑