Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913504,6160TA48.6% 32.5 / 29.3 37L27F (TTA→TTT) fsrputative fosmidomycin efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (17/2);  new base A (0/18);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 1.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGAGCTGGAAGGTGAGGGTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACT  >  NC_000913/504481‑504760
                                                                                                                                       |                                                                                                                                                 
ggCGAGCTGGAAGGTGAGGGTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGAt                                                                                                                                    <  1:747129/151‑1 (MQ=255)
                      aTCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACg                                                                                                             >  2:14960/1‑151 (MQ=255)
                         aTGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAg                                                                                                          <  2:72384/151‑1 (MQ=255)
                                 cTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGcgccc                                                                                                  >  1:156842/1‑151 (MQ=255)
                                    cATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGcgcccgcc                                                                                               >  1:650859/1‑151 (MQ=255)
                                         aTGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAgg                                                                                          >  1:64990/1‑151 (MQ=255)
                                           gTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCt                                                                                        >  1:467151/1‑151 (MQ=255)
                                              aaaaaaatTTCTGAGGGAAGAAGCGTATAACCCGCCAGAACCAGCGATTGTATCTTGTCTTTCAGCAGAGGTGAGGGCCTGATACCACCAAAAACACCAAA‑GGTGTTGGGGGCCTTGGTCGTTGACGCAGCCGCCCCCGCCACAGGCTgg                                                                                      <  2:486315/146‑1 (MQ=255)
                                                   aaaTTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgttgt                                                                                >  2:670074/1‑151 (MQ=255)
                                                            ctgaAGCAGCGAATAAATCGCCAGAACAAGGGATTGGATCATGTGGTTCAGCAGATGTGAGAGGCTAATAGCACAAAAAATCCAAAACGTTGTT‑CGGGCTTTGTTCTTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCAt                                                                        <  1:264933/150‑1 (MQ=255)
                                                               aaGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGcc                                                                    >  1:444786/1‑151 (MQ=255)
                                                                      gaaTAAACCACAAGACTCCGAGATTGAATCTTGTGTTACAGAGGATGAAAGAGGGGGAAAGCAAAAAAAATACAAAACGGTGTC‑CGGCCCTTGCCCGTGCGCCCAGGACGACCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGa                                                              <  2:344484/148‑1 (MQ=255)
                                                                        aaaaaTCGCAAGAACCAGCGATTGATTCATGTCGTTCAGCAGAGGGGGGGGGCTGATACCACCAAAAATCCAAACCGATGTT‑GGGGCTTGGGTCGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAg                                                           <  2:26587/149‑1 (MQ=255)
                                                                          aaaTCGCGAAAATCAGCGATGGGATCATGTCGTTCAGCGGGTGGGGGGGGCTGATAGCCCAAAAAATCCCAAACGATGTT‑CGGGCTTTGTTTTTGGACGCAGCCGCGCCCGCCACAGGCTGGGCTTGTTCACTCATTGCCATAGGAAAGTc                                                         <  1:83811/151‑1 (MQ=255)
                                                                          aaaCCGCAAAAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGGGGCTGATAGCACCAAAAAAACCAAACGATGTT‑CGGCCCTTGGTCGTTGACGCAGCCCCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGt                                                          <  1:305506/150‑1 (MQ=255)
                                                                               gCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACttt                                                    >  2:320078/1‑151 (MQ=255)
                                                                                 cAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACttttt                                                  >  2:437479/1‑151 (MQ=255)
                                                                                  aaaaTCAGGGTTGGTATCTTGTCGTTCAGAGGATGGGAGGGGCTGATAGCACAAAAAATCCCAAAGGATGTG‑GGGGCTTGGTTCGTTGCCGCCGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACttttt                                                  <  2:717410/148‑1 (MQ=255)
                                                                                  aGAATCAGCGTTTGGATCATGTCGTTCAGCAGATGGGAGGGGTTGATAGCCCCAAAAATACAAAACGATGTT‑GGGGCTTTGGTCGTTGCCGCGGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACttttt                                                  <  1:766423/150‑1 (MQ=255)
                                                                                  aGAATCAGCGATTGGCTAATGTCTTCCAGAGGATGGGAGGGGGGGATAGCACAAAAAATACCAAACGTTGTT‑CGGCTTTTGTTCTTGGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACttttt                                                  <  2:93304/150‑1 (MQ=255)
                                                                                  aGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTc                                                 >  2:419166/1‑151 (MQ=255)
                                                                                   gAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTc                                                 >  1:609359/1‑150 (MQ=255)
                                                                                    aaTCGGCGATGGGATCATCTCGTTCAGCAGAGGGGAGGGGCTGACACCACCAAAAACACAAAACGAGGGG‑GGGTCTTTGGTCGTTGCCGCGGCCGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCa                                                <  2:126749/150‑1 (MQ=255)
                                                                                    aaTCAGGGTTTGGATAATGTCGTTAAGCAGATGGGAGGGGCTGATAGCACCAAAAATCCAAAACGTTGTT‑CGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCa                                                <  1:562270/150‑1 (MQ=255)
                                                                                               gaataaTGTCGTTCAGCGGATGTGAGAGGCTGATCGCACAAAAAATACAAAACGTTGTT‑GGGGCCTGGGTGTTGGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATg                                     <  1:283291/145‑1 (MQ=255)
                                                                                                  tCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaa                                  >  1:506408/1‑150 (MQ=255)
                                                                                                   cATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaa                                  >  2:517424/1‑149 (MQ=255)
                                                                                                   cATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTaa                                  >  1:158636/1‑149 (MQ=255)
                                                                                                          ttCAGCAGATGTGAGAGGCTGATAGCCCCAAAAATACAAAACGATGTT‑CGGGCTTTGGTCGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAAt                             <  1:437482/147‑1 (MQ=255)
                                                                                                          ttCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATc                         >  1:789394/1‑151 (MQ=255)
                                                                                                             agcagATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCtt                       >  2:644352/1‑150 (MQ=255)
                                                                                                              gaggaggtggggggCTGATAGCACCAAAAATCCCAACTGATTTG‑CGGGCTTTGGTCGTTGCCGCGGCCGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTa                      <  2:448100/138‑1 (MQ=255)
                                                                                                                 gATGTGAGAGGCTGATAGCACCAAAAATCCAAAACGATGTT‑GGGGCTTTGGTTGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAttt                   <  1:349582/150‑1 (MQ=255)
                                                                                                                      gaggGGCTGATAGCACCAAAAATACCAAACGTTGTG‑GGGGCTTTGGTCGTGGCCGCCGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGa               <  2:643904/149‑1 (MQ=255)
                                                                                                                               aTAGCCCCAAAAATCCAAAACGTTGTG‑GGGGCTTGGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGa    <  1:612580/151‑1 (MQ=255)
                                                                                                                               aTACCACCAAAAATACCAAACTTTGTT‑CGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCa      <  2:433716/149‑1 (MQ=255)
                                                                                                                                 aGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACt  >  1:164099/1‑151 (MQ=255)
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GGCGAGCTGGAAGGTGAGGGTTATCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTT‑CGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACT  >  NC_000913/504481‑504760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑