Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913616,5640AT43.8% 43.0 / 13.2 33Y803F (TAT→TTT) entFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (2/16);  new base T (14/0);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 5.09e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAG  >  NC_000913/616416‑616709
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gATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTa                                                                                                                                                   <  2:224948/149‑1 (MQ=255)
   gTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTAtctc                                                                                                                                               <  1:397408/150‑1 (MQ=255)
      aGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGCGGTCTTtctctccc                                                                                                                                             >  1:382516/1‑147 (MQ=255)
                     tttGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGgcg                                                                                                                             <  2:7149/150‑1 (MQ=255)
                         gCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAggg                                                                                                                        <  2:296557/151‑1 (MQ=255)
                         gCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTTTCTTCCCGGCCGTCTACTGgcggcggg                                                                                                                        >  2:329793/1‑146 (MQ=255)
                          cGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTTTTTCTCTGGCATTTAAACGGGGCAggg                                                                                                                        >  1:160051/1‑150 (MQ=255)
                                    gCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGGTCTTTTTTTCACTGGCCTTCAACTGGCGCAGGGGTATCTCgg                                                                                                               >  1:77586/1‑149 (MQ=255)
                                    gCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGAc                                                                                                             <  1:638465/151‑1 (MQ=255)
                                          gTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTTACTGGCCTTCAACTGGCGCCGGGCTCTTTCCGGCGcccc                                                                                                        >  1:489499/1‑150 (MQ=255)
                                          gTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTCTCTCCCTTGCATTTAAATGGGGCGGGGGGATCTCGGGCGccccc                                                                                                       >  2:398103/1‑150 (MQ=255)
                                                  cagcagTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGAcc                                                                                               <  2:565616/151‑1 (MQ=255)
                                                   agcagTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTTTCTCACTGGCCTTTCAATGGCGGCGGGGGCTCTCTGACGCCCCCCTCTGAcc                                                                                               >  1:672742/1‑150 (MQ=255)
                                                         gtgCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGGCTCTTTCTCCCTGGCACTTAACCTGCGGGGGGGGGTTTCGGGCGCCCCGCCCTGACCGcccccc                                                                                        >  2:199799/1‑147 (MQ=255)
                                                              gATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCttt                                                                                   <  1:258401/151‑1 (MQ=255)
                                                               aTTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGGTTTCTTTTTCCCTTGGATTTAAATGGCGCAGGGGGATCTCGGGCCCCCCCCTCTTACCCCCCGcc                                                                                        >  2:236946/1‑145 (MQ=255)
                                                                           gTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCtt                                                                      <  1:671269/151‑1 (MQ=255)
                                                                           gTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCCCTTGCGTTCAACTGGGGCAGGGCTATCTCGGGCGCCCCCCTCTGACCCCCCCCCCCTTTTTTTTCGATcc                                                                        >  1:722971/1‑149 (MQ=255)
                                                                             aTGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCtttt                                                                    <  1:558834/151‑1 (MQ=255)
                                                                             aTGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCtttt                                                                    <  2:296346/151‑1 (MQ=255)
                                                                             aTGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCttt                                                                     <  1:89364/150‑1 (MQ=255)
                                                                             aTGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCCCTGGCCTTTTACTGGGGCCGGGGTATCTCCGGCGCCCCCCTCTTACCCCCCGCCCCTTTTTTTCCCCTCCtttt                                                                    >  1:221457/1‑151 (MQ=255)
                                                                              tGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGGTCTCTTTCTTACTGGCATTCAAATGGCGCAGGGCTATCTCCGGCGCCCCGGTCTGACCGCCAGCCGCTTTTTTTCCGATCCttttt                                                                   >  1:591026/1‑150 (MQ=255)
                                                                                gAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGGTTTCTTTTTCACTTGCATTCAACTGGCGCCGGGGTATCTCGGGCGCCCCCCTCTGACCCCCCGCCGCCTTTTTTCCGATCCtttt                                                                    >  1:82827/1‑148 (MQ=255)
                                                                                 aaTACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCACTGGGAATCCACTGGGGGAGGGGTCATTCAGACGCCCCCGTCTGACCCCCCCCCGCTTTTTTgtcccgcctttttgcc                                                                >  1:253022/1‑136 (MQ=255)
                                                                                                aTTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATgt                                                 <  2:666516/151‑1 (MQ=255)
                                                                                                      gATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATGTCTTTCTCACCCGCATTTAACTTGGGCGGGGCGATCTCCGACGCCCCCCTCTTACCGCCCCCCCCCTTATTGCCGATTCTTTTTCCCCCAGGGAACgg                                                     >  1:598290/1‑141 (MQ=255)
                                                                                                        tGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCACCGGGCTTCAACTTGCGCAGGGGTATTTCGGGCGCCCCCCTCTGACCCCCCCCCCCTTTTTTTTCCCTCCCTTTTCCCCCCGGGAACGGgtgttacctacc                                         >  1:116613/1‑143 (MQ=255)
                                                                                                              gatgCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGgaga                                    <  2:77586/150‑1 (MQ=255)
                                                                                                              gatgCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGAc                                   <  1:398106/151‑1 (MQ=255)
                                                                                                               atgCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACg                                  <  2:791829/151‑1 (MQ=255)
                                                                                                                             gccgGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCgctggctg                    <  1:421998/151‑1 (MQ=255)
                                                                                                                               cgGGTGTGGCGGGTGATCTCTATCTCACTGGCCTTCAACTGGCGCCGGGGGATTTCGGGCGCCCCCCTCTTGCCCCCCGCCCCCTTTTTTTCGGTCCTTTTTCCCCACGGGAAAGGGGGTATCGCACCCGAGACGGTGCCCcccggcgcg                   >  2:687519/1‑147 (MQ=255)
                                                                                                                                         gggTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGcgcg        >  2:440466/1‑151 (MQ=255)
                                                                                                                                                  ctATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAg  <  1:412193/148‑1 (MQ=255)
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GATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGTGGAG  >  NC_000913/616416‑616709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑