Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC45.2% 60.0 / 10.9 42T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (16/7);  new base C (0/19);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 2.01e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATG  >  NC_000913/1376361‑1376643
                                                                                                                                                  |                                                                                                                                          
ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                       <  1:429036/150‑1 (MQ=255)
   ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                   <  1:449764/151‑1 (MQ=255)
   ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                   <  2:312381/151‑1 (MQ=255)
        gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCTTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGCATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGc                                                                                                                               <  2:556733/150‑1 (MQ=255)
                          ggccGGCGACCGAAAAATGGTGAGTGTTTCCCTGCGCGTACGGGAACA‑GGCCGCGGCGCGCCCCGGACCGGTGTTTTATCTCGAACTGTTAAC‑CCGTTATCAGAATCATATGACAACCCCCCCCGCCGATGCCCGCCGTTACAtcgtcg                                                                                                              <  2:486294/146‑1 (MQ=255)
                          ggACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGa                                                                                                             >  1:709256/1‑150 (MQ=255)
                              ggCGCCCGAAAATGGGTGTGGGATCCCCGGCGCTTACTGGAACG‑GGTCGCGGCGCGTACGGGACCCGTGTTTTA‑CCCCAACCCTTAAACCCGCTACCAGAACAATATAAACACCCCCCCCGCCGATCCCCGCCGTTACATCGTCGaaaa                                                                                                          <  2:207807/149‑1 (MQ=255)
                              ggCGACCGAAAAAGGGTGAGTGATTCCCTGCGCGTACTGAAACA‑GGTCGCCGCGCGTACCGAAACCGTGGTGTATCTCGACCCGTTAAA‑CCGCTATCAGGATAATATGACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                        <  1:451776/151‑1 (MQ=255)
                              ggCGACCGAAAAAGGGTGAGTGATTCCCTGCGCGTACGGGAACG‑GGTCGCCGCGCGTCCCGGACCCGGGGTGTCTCTCGAACCGTAAAC‑CCGCTATCAGGATAATATGATCAACCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                        <  2:681548/151‑1 (MQ=255)
                                       aaagTGGTGAGTAATTCCCTGGGGGTATGGGAACA‑GGCCGCGGCGCTTCCGGGACCGGTGTTTTATCCCGACCCGTTAAC‑CCGTTACCAGGATAATATGACAAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                 <  2:378979/149‑1 (MQ=255)
                                       aaagTGGGGAGTGTTCCCCTGCGCGTACGGGAACA‑GGTCCCCGCGCTTACCGGACCGGGGGTGTTTCCCGACCCGTTAAC‑CCGTTATAAGGATAATATGAACACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTa                                                                                                  <  2:214380/148‑1 (MQ=255)
                                         aaTGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                             >  1:406737/1‑151 (MQ=255)
                                          aTGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                            >  1:314367/1‑151 (MQ=255)
                                                   gtttCCCTGCGCGTACTGAACCG‑GGTCGCCGCGCGTCCCGGAACCGTGGTTTATCTCGACCCTTAAAA‑CCGCTATCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                     <  1:435148/147‑1 (MQ=255)
                                                   gtttCCCTGCGCGTACGGGAACA‑GGCCGCCGCGCGTCCCGGACCCGTGGTGTATCTCGACCCTTTAAC‑CCCCTATCAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                   <  1:602947/149‑1 (MQ=255)
                                                    aTTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                  >  1:118063/1‑151 (MQ=255)
                                                      tCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCg                                                                                >  1:605873/1‑151 (MQ=255)
                                                                ttcggaaaCA‑GGTCGCCGCGCTCACCGAAACGTTGGTGTATCTCGAACTGTTAAC‑CCGCTATCAGGATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                       <  1:388282/144‑1 (MQ=255)
                                                                     gAACAGGGTCGCCGCGTTTACGGGAACGGTGGTGTATCTCGAACCTTTAAA‑CCTCTATAAGAATCATATTCCAACCACCCCCGCGGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATca                                                                   <  1:734442/150‑1 (MQ=255)
                                                                           gttccccgcGCGACCCGGACCCGGGTTGTATC‑CCAACCTTTAAACCCCCTATCAGAATAATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                              <  2:406052/144‑1 (MQ=255)
                                                                           gttCGCCGCGCGTGCCGGACCCTTGGGTTATTCGGAACCTTTAAC‑CCCCTACCCGGTTAGTTAAATCACCCCCCCCGCGGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                <  1:752163/145‑1 (MQ=255)
                                                                           ggTCGCCGCGCGTACGGGACCCGGGGTGTATCTCGACCCGTTAAC‑CCGCTATCAGGATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                              <  1:252531/149‑1 (MQ=255)
                                                                               gccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                        >  2:698221/1‑151 (MQ=255)
                                                                                cggcgcgcACGGAACCCGTGGTTTATC‑CCAACCCTTAAACCCGCTTTAAGAATAATATAACCACCACCCTCGCCGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                        <  1:664530/148‑1 (MQ=255)
                                                                                ccgcGCGTACCGGAACCGTGGTGTATCTCGACCCGTAAAA‑CCGCTATCAGGTTCATATGACCAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAatat                                                          <  1:787338/148‑1 (MQ=255)
                                                                                  gcgcgTCCCGGACCGGTGGTTTATCTCGACCCTTAAAC‑CCGCTATAAGGAAAATATAAACAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAatat                                                          <  2:521085/146‑1 (MQ=255)
                                                                                    gcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                    >  1:700330/1‑150 (MQ=255)
                                                                                     cgTACCGAACCTGTGGTGTATCCCGACCCGTTAAA‑CCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGaagaag                                                  <  1:614542/151‑1 (MQ=255)
                                                                                         ccgaacccGGGGTGTATCTCGACCCGTAAAC‑CCGCTATCAGAATAATATGACAAACCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                   <  1:525268/140‑1 (MQ=255)
                                                                                          cGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                              >  1:413401/1‑150 (MQ=255)
                                                                                           ggaccggtggttTATC‑CCAACCGTTAAACCCGCTAACAGAATCATATAACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                             <  1:148382/144‑1 (MQ=255)
                                                                                             aaCCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                          >  1:653738/1‑151 (MQ=255)
                                                                                                cGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcg                                       >  2:618548/1‑151 (MQ=255)
                                                                                                              accggTTAAC‑CCGCTATAAGGATAATATAATCAACCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                               <  2:548025/141‑1 (MQ=255)
                                                                                                               aCCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                        >  2:588186/1‑151 (MQ=255)
                                                                                                               aCCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                        >  2:10854/1‑151 (MQ=255)
                                                                                                                ccGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                        >  2:54625/1‑150 (MQ=255)
                                                                                                                      aacccccTATCAGGATCATATGAACAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                  <  2:353955/145‑1 (MQ=255)
                                                                                                                                  ggATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg        <  1:525212/149‑1 (MQ=255)
                                                                                                                                     tCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt   >  1:63863/1‑151 (MQ=255)
                                                                                                                                      cATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg  >  2:446788/1‑151 (MQ=255)
                                                                                                                                       atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg  >  1:393584/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
CCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACA‑GGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATG  >  NC_000913/1376361‑1376643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑