Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	809,340	C→G	intergenic (‑83/‑4)	bioA ← / → bioB	7,8‑diaminopelargonic acid synthase, PLP‑dependent/biotin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	809,340	0	C	G	100.0%	153.4 / NA	39	intergenic (‑83/‑4)	bioA/bioB	7,8‑diaminopelargonic acid synthase, PLP‑dependent/biotin synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/19);  total (20/19)

GTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTG  >  NC_000913/809196‑809478                                                                                                                                                 |                                                                                                                                           gTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATg                                                                                                                                      <  2:66892/151‑1 (MQ=255)       gATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCAc                                                                                                                                >  1:482853/1‑151 (MQ=255)       gATGTGTATAGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTc                                                                                                                                  <  1:234835/149‑1 (MQ=255)              aTGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCa                                                                                                                          <  1:237511/150‑1 (MQ=255)                        gATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACAtt                                                                                                               >  2:218203/1‑151 (MQ=255)                           atGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTc                                                                                                            <  2:318111/151‑1 (MQ=255)                               cGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCaa                                                                                                        <  1:257100/151‑1 (MQ=255)                                   ggTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTc                                                                                                     <  1:499552/150‑1 (MQ=255)                                      cAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTcac                                                                                                   >  1:719120/1‑149 (MQ=255)                                       aaaGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTcac                                                                                                   >  1:295151/1‑148 (MQ=255)                                         aGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAat                                                                                              <  1:258185/151‑1 (MQ=255)                                             aaGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAattatt                                                                                          <  1:380122/151‑1 (MQ=255)                                             aaGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAattatt                                                                                          >  1:431676/1‑151 (MQ=255)                                                aTCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGa                                                                                       <  2:371198/151‑1 (MQ=255)                                                  cGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGaa                                                                                      <  2:69944/150‑1 (MQ=255)                                                   gTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGaaa                                                                                     >  2:45686/1‑150 (MQ=255)                                                       gTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCg                                                                                >  2:269460/1‑151 (MQ=255)                                                            cATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGc                                                                            <  1:260749/150‑1 (MQ=255)                                                                       tGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGt                                                                >  1:762360/1‑151 (MQ=255)                                                                        gTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGtt                                                               <  2:249025/151‑1 (MQ=255)                                                                         tAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGttt                                                              >  1:675716/1‑151 (MQ=255)                                                                            aCCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGaa                                                           <  1:727175/151‑1 (MQ=255)                                                                             ccAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGa                                                            >  1:246553/1‑149 (MQ=255)                                                                                 aTTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcgc                                                       >  2:102139/1‑150 (MQ=255)                                                                                  ttGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcgc                                                       >  1:673565/1‑149 (MQ=255)                                                                                      aaaGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAgg                                                 >  1:612450/1‑151 (MQ=255)                                                                                        aGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTg                                               <  1:332771/151‑1 (MQ=255)                                                                                           tttAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCAt                                            >  2:223257/1‑151 (MQ=255)                                                                                             tAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCg                                          >  1:287659/1‑151 (MQ=255)                                                                                                    aCAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCAt                                   >  2:369681/1‑151 (MQ=255)                                                                                                      aaGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCAttt                                 <  2:431673/151‑1 (MQ=255)                                                                                                         tCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCg                               <  1:137233/150‑1 (MQ=255)                                                                                                           tACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATc                            >  2:433709/1‑151 (MQ=255)                                                                                                              acCGAATTAACAACAAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTc                         >  2:315803/1‑151 (MQ=255)                                                                                                                       acaacaAAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGc                <  2:404522/151‑1 (MQ=255)                                                                                                                         cacaaAAAACACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGc                >  2:270591/2‑149 (MQ=255)                                                                                                                             aaaaaCACGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCa          <  1:163192/151‑1 (MQ=255)                                                                                                                                    cGTTTTGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGtt   >  2:254821/1‑151 (MQ=255)                                                                                                                                      ttttGGAGAAGGCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTg  <  2:480459/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                           GTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTG  >  NC_000913/809196‑809478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑