Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,687	C→A	V262F (GTT→TTT)	sapB ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,687	0	C	A	100.0%	157.5 / NA	42	V262F (GTT→TTT)	sapB	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/20);  total (22/20)

GTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACAT  >  NC_000913/1354541‑1354830                                                                                                                                                   |                                                                                                                                                gTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACAtt                                                                                                                                             <  1:27020/151‑1 (MQ=255) gTTAGCCATGGCACCCAAAATAACAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCGGCTGGCGGATGGCGTTAATTAACCAGCGCACTAAACCCGGCCAGCTAAAAAACAtt                                                                                                                                             <  1:648735/151‑1 (MQ=255)   tAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGGTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACAttt                                                                                                                                            >  2:76650/1‑150 (MQ=255)      ccATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGt                                                                                                                                        <  1:657922/151‑1 (MQ=255)         tGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGat                                                                                                                                     >  1:294103/1‑151 (MQ=255)                    atatCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCg                                                                                                                          >  1:464984/1‑151 (MQ=255)                     tatCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCg                                                                                                                          >  2:621090/1‑150 (MQ=255)                      atCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGt                                                                                                                         <  2:499343/150‑1 (MQ=255)                      atCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTc                                                                                                                        <  1:697175/151‑1 (MQ=255)                            aaTCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtg                                                                                                                  >  1:706437/1‑151 (MQ=255)                               cACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtggtg                                                                                                               >  1:5877/1‑151 (MQ=255)                               cACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtggtg                                                                                                               >  1:743060/1‑151 (MQ=255)                               cACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtggtg                                                                                                               <  1:182320/151‑1 (MQ=255)                                aCGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtggtgg                                                                                                              >  1:25839/1‑151 (MQ=255)                                aCGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtggtgg                                                                                                              >  2:24884/1‑151 (MQ=255)                                 cGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGa                                                                                                             <  2:11086/151‑1 (MQ=255)                                                ccAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGccc                                                                                                >  2:491118/1‑149 (MQ=255)                                                       gCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGa                                                                                       <  2:416439/151‑1 (MQ=255)                                                        ccACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGaa                                                                                      <  2:226926/151‑1 (MQ=255)                                                             cacCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGGCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAAcc                                                                                 <  2:31261/151‑1 (MQ=255)                                                                   cACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACcggcgg                                                                            <  1:650472/150‑1 (MQ=255)                                                                         ggcggAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGGAAACTGTAAGACCAGGCGAGGAATAACCGGCGGTAAcgcg                                                                     >  2:745937/1‑151 (MQ=255)                                                                                                 ctgGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCaa                                              >  2:160037/1‑150 (MQ=255)                                                                                                    gCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAAt                                           >  2:488697/1‑150 (MQ=255)                                                                                                    gCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATa                                          <  2:223792/151‑1 (MQ=255)                                                                                                       gATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTa                                       <  1:348618/151‑1 (MQ=255)                                                                                                        aTGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTa                                       >  1:245734/1‑150 (MQ=255)                                                                                                           gCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAgc                                   <  1:162250/151‑1 (MQ=255)                                                                                                            cGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAgcg                                  <  1:549772/151‑1 (MQ=255)                                                                                                             gTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAgcgc                                 >  1:127271/1‑151 (MQ=255)                                                                                                                 attaaCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACa                             <  2:519927/151‑1 (MQ=255)                                                                                                                              cTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgc                >  1:780168/1‑151 (MQ=255)                                                                                                                               tAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGt                   <  2:18573/147‑1 (MQ=255)                                                                                                                                 aaCCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgc             >  2:445235/1‑151 (MQ=255)                                                                                                                                 aaCCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgc             >  2:472308/1‑151 (MQ=255)                                                                                                                                  aCCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgcc            >  2:324891/1‑151 (MQ=255)                                                                                                                                  aCCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgcc            <  2:421206/151‑1 (MQ=255)                                                                                                                                   cccGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgccg           <  2:743057/151‑1 (MQ=255)                                                                                                                                      ggCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCtt        >  1:499439/1‑151 (MQ=255)                                                                                                                                          aGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTa     <  2:28206/150‑1 (MQ=255)                                                                                                                                           gCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTAc    >  2:219881/1‑150 (MQ=255)                                                                                                                                             tAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACAt  >  2:561286/1‑150 (MQ=255)                                                                                                                                              aaaaaaCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACAt  >  1:603142/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                GTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACAT  >  NC_000913/1354541‑1354830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑