Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,036,906 | 0 | T | A | 47.4% | 64.9 / 33.6 | 57 | L53* (TTA→TAA) | hyaF | hydrogenase‑1 protein nickel incorporation factor |
Reads supporting (aligned to +/- strand): ref base T (26/4); new base A (1/26); total (27/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.40e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGC > NC_000913/1036756‑1037053 | aaaCTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGtt < 1:12507/151‑1 (MQ=255) aaCTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGtt > 2:112731/1‑150 (MQ=255) tgctgGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTacaac > 2:650230/1‑150 (MQ=255) gctgGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTacaaca > 2:1039698/1‑150 (MQ=255) ggCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACt < 1:513380/151‑1 (MQ=255) gCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GCGTATTGCGGCCCTGGAGCAA‑TGTGATCACACCCCGCAAGGGATTTCGCTGTAAAACGGGTTacaac > 2:106645/1‑143 (MQ=255) cAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGcc > 1:436313/1‑151 (MQ=255) aaCCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGcc > 1:281031/1‑150 (MQ=255) ccGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCTACTAAGCGAACGCCAAc < 2:638776/151‑1 (MQ=255) gAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAAccg > 1:1036813/1‑151 (MQ=255) gATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAAccgccg > 2:198825/1‑150 (MQ=255) gATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGt > 2:831501/1‑151 (MQ=255) cAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTg > 2:207600/1‑151 (MQ=255) cATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTg > 1:528196/1‑151 (MQ=255) ataactccccggCCGCTCCCCTGTCAGGTAAATGATGACCC‑GAGTTTGGGGGCCGTGGAGAAG‑TGTCCCCACAGCCCGGGGGTGATGGCGTTGTTAAACGGGTTACAACATAAACTAAGCGAACCCCAACCGCCGTTGGGCGAGGTGCTg < 1:184931/139‑1 (MQ=255) gAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGc > 1:566477/1‑150 (MQ=255) aacccccggCCGATCCCGTGTCGGGTAAATGATAAACC‑GAGTAGGGCGCCCGGGGAGAAA‑TGTCCCCCCAGCCCGGGGGTGATGGCGTTGTAAAACGAGTAACAACAAAAACAAAGCGAACGCAAACCGCCGTTGGGCGAGGTGCTgg < 2:714993/140‑1 (MQ=255) aacccccTGCCGTCCCCCTGTCGGGGAAATGATGAACC‑GAGTAGGGGGCCCGTGGAGAAA‑GGTCCTCACACCCCGCAGGTGATTGCCTTGTAAAACGGTTAAAAACATAAACAAAGCAACCGCAAACCGCCGTTGGGCGAGGTGCTGGc < 1:959939/143‑1 (MQ=255) aaTCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGc > 2:641101/1‑149 (MQ=255) ccccTGCCGATCCCCTGTCAGGTAAATGATAACCC‑GAGTATGGC‑GCCCCGGAGGAATTGTGCTCACAGCCCGCGGGTGTTTGCGCTGTAAAACGATTAACAACACCAACAAAGCAAACCCCAACCGCCGTTGGGCGAGGTGCTGGCAGt < 1:958066/146‑1 (MQ=255) cACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCg > 2:922808/1‑150 (MQ=255) cTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGAGCTGGCAGTCGATc > 2:1005134/1‑151 (MQ=255) ccaaTCCCCTGTCAGGTAAATGATGACCC‑GAGTAGGGCGGCCCGGGAGAAA‑TGTCCTCACAGCCCGCAGGTGATGGCGCTGTAAAACGAGTTACAACATCAACAAAGCGAACGCAAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTg < 1:1058377/147‑1 (MQ=255) ccGATCCCCTGTCGGGTGAATGATGAACG‑GAGTAGGGCGGCCGTGGAGAAA‑TGTGCTCACAGCCCGCAGGTGTTTGCGTTTTAAAACGAGTTAAAACATCAACAAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATc < 1:552953/148‑1 (MQ=255) gATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGtt > 2:519334/1‑150 (MQ=255) gATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGtt > 1:301188/1‑150 (MQ=255) ccccTGTCGGGTAAATGATGAACC‑GAGTAGGGCGCCCCTGGAGAAA‑TGTCCTCACCCCCCGGGGGTGTTTGCGTTGTAAAAGGGGTTACAACAACAAGAAAGCAACCGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGAt < 2:352507/140‑1 (MQ=255) cACCTGCAAGGTAAATGA‑GAACCGGAGTAGGGGGGCCCGGGAAAAA‑TGTGCCCACACCCCGCGGGTGATGGCGTTTTAAAACGATTAACAACATCAACAAAGCAAACCCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTaaa < 1:810771/150‑1 (MQ=255) cccTGCCAGGTAAAGGAGAACCC‑GAGTTGGGCGCCCTTGGACCAA‑TGTCCCCACCGCCCGCGGGTTATGGCGTTGTAAACGGAGTTACAACAACAACAAGGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTaa < 2:73131/147‑1 (MQ=255) aCCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAAtc > 1:734257/1‑151 (MQ=255) ccggtgaaggaaaTGGTGACCG‑GAGTAGGGGGGCCCGGGAGCAA‑GGGCCTCGCGCCCGGCAGGTGATTGCTTTTTTAAGCGAGTTAAAACAACAAGAAGGGGAGCGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGtt < 2:381012/134‑1 (MQ=255) ccTGTCAGGAAAATGATGACCC‑GAGTATGGGGCCCCTGGGGCAA‑TGCGCTCCCGCCCCGCGGGTGATTGCGCTTTAAAACGATTTACAACATCAACTAGGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGtt < 1:298976/145‑1 (MQ=255) gtcgggaaattaatgaCCG‑GAGTAGGGCGCCCCTGGAGAAT‑TGCCCCCACGGCCCGCAGGTTTTGGCGCTGTAAAACGAGTAACAACACCAACAAGGCGACCCCAAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAAtctc < 1:438643/137‑1 (MQ=255) tCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAAc > 1:683723/1‑151 (MQ=255) ggTGAATGATGAACG‑GAGTGGGGCGGCCCGGGAGCAA‑TGTCCCCACGCCCCGCAGGTGATGGCGTTGTAAAACGAGTTACAACACCAACTAAGCAACCCCCACCCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGcc < 2:300530/151‑1 (MQ=255) ggTGAATGATGAACC‑GAGTAGGGCGCCCCTGGAGAAA‑TGTGCTCCCGGCCCGCAGGTGTTTGCGCTGTAAAACGATTTACAACATCAACAAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGc < 1:590532/150‑1 (MQ=255) ggTGAATGATAACCC‑AAGTAGGGCGGCCCGGGAGAAT‑TGTGCCCACCCCCCGGGGGTGGTTGCCCGTTAAAAGGATTTACAACAACAACTAGGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCaa < 1:792932/147‑1 (MQ=255) tgaACG‑GAGTGGGGCGCCCTGGGAAAAT‑TGTGCTCACAGCCGGAGGGTTATGGCGTTTTAAAACGTTTTAAAACATCAACTAAGCGAACCCCATCCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGAt < 1:582898/148‑1 (MQ=255) cc‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACt > 2:911485/1‑151 (MQ=255) cggAGTAGGGCGGCCCGGGAGAAA‑TGTCCCCACGGCCCGCGGGTGTTTGCGTTGTAAAACGAGTTACAACATAAACAAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATc < 1:615982/143‑1 (MQ=255) aTGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATc > 2:628276/1‑150 (MQ=255) ggcggcCCGGGAGCAA‑TGTGCCCACACCCCGCAGGTGATGGCGTTGTAAAACGAGTAAAAACAACAACAAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCa < 1:11071/149‑1 (MQ=255) ggcgccCCGGGAGAAA‑TGTCCCCCCACCCCGGAGGTGTTTGCGTTTTAAAAGGAGTTACACCATCAACAACGCGACCCCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAAt < 2:880628/151‑1 (MQ=255) cTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTtc > 2:825852/1‑151 (MQ=255) ggAGAAA‑TGTGCCCACAGCCCGCAGGTGTTGGCGTTGTAAAACGAGTAACAACATAAACAAAGCGACCGCCAGCCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTtc < 1:104287/149‑1 (MQ=255) gtgCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCggggaagg > 2:830309/1‑151 (MQ=255) gtccccacaCCCCGGAGTTGTTTCCGTTTTAAACCGATTTACAACACCAACAAACCGACCGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCggggaa < 1:1054378/144‑1 (MQ=255) tgCTCCCACCCCGCGGGTGATTGCGTTGTAAAACGAGTTACAACATCAACTAAGCAAACCCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCg < 2:748486/143‑1 (MQ=255) cccacaGCCCGCGGGTGATTGCGTTTTAAAACGAGTTACAACATCAACAAAGCAAACCCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCggggaagggg < 1:367903/148‑1 (MQ=255) tCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCggggaagggga > 2:676132/1‑150 (MQ=255) cccGGAGGTATTTGCGCTATAAACGGTGTTACAACACCAAAAAGGCCAACGCCACCCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCggggaaggggaa < 1:900418/145‑1 (MQ=255) ccggggTGATCGCGCTTTAAAACGAGTAACACCATCAACAACGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAg < 2:1078664/146‑1 (MQ=255) ggTGATGGCGTTGTAAAACGATTAACAACATAAAATAAGCGACCCCAAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTg < 2:142330/148‑1 (MQ=255) gTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTgcgc > 2:22685/1‑150 (MQ=255) aTTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTc > 1:639898/1‑151 (MQ=255) gcTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTcagc > 1:710908/1‑149 (MQ=255) tGTAAACCGAGTAAAAACACCAACAAAGCGACCGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTcagcag < 1:667936/149‑1 (MQ=255) tGTAAAACGAGTTACAACATCAACTAAGCGACCGCAAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTca < 1:604308/145‑1 (MQ=255) gTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGc > 2:267330/1‑150 (MQ=255) | AAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGATCACCTGTCAGGTGAATGATGAACC‑GAGTATGGCGGCCCTGGAGCAA‑TGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAAACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGC > NC_000913/1036756‑1037053 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |