Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,505	0	A	C	42.9%	115.6 / 24.6	70	T158P (ACC→CCC)	ycjR	putative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (34/6);  new base C (0/30);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 3.60e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGT  >  NC_000913/1376355‑1376644                                                                                                                                                           |                                                                                                                                            cGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAaca                                                                                                                                             <  1:115054/151‑1 (MQ=255)    ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACAccc                                                                                                                                          >  1:765966/1‑151 (MQ=255)       ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                        <  2:106735/150‑1 (MQ=255)       ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCg                                                                                                                                       <  1:709914/151‑1 (MQ=255)           cgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                    >  1:327550/1‑150 (MQ=255)           cgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                    >  2:852813/1‑150 (MQ=255)            gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGa                                                                                                                                   >  2:156112/1‑150 (MQ=255)            gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                  <  2:487762/151‑1 (MQ=255)               gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGcc                                                                                                                               <  2:856461/151‑1 (MQ=255)                       cgcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGtt                                                                                                                        >  2:512181/1‑150 (MQ=255)                        gcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTAc                                                                                                                      >  1:7874/1‑151 (MQ=255)                           tAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                   >  2:1003947/1‑151 (MQ=255)                              ccTGGCGGGCGCCCGAAAAATGGTGAGTTTTCCCCTGCGCGTACGGGAACAGGCCCCCGCGCTTACCGGACCC‑GTGTTT‑TTT‑CTCGACCCTTTAAC‑CCGCTACAAGGATCATATGAAAAACCCCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                   <  2:549086/148‑1 (MQ=255)                                gggACGGCGCCCGAAAAATGGTGAGTGATCCCCGGCGCGTCCGGAAACAGGTCGCCGCGCGTCCCGGACCC‑GTGGTG‑TAT‑CTCGACCCTTAAAC‑CCGCTACAAGGATAATATGACCAACCCCCTCGCCGATGCCCGCCGTTACAtcgtc                                                                                                                <  1:203427/148‑1 (MQ=255)                                 gggcGGCGCCCACAAAGTGGTGAGTGTTTCCCTGCGGGTTCTGAAACAGGTCGCCGCGCGT‑CCCGAACC‑CGGGTGTTAT‑CTCGACCCTTAAAC‑CCGTTACCAGGATCATATGACCACCCCCCTCGCCGATGCCCGCCGTTACAtcgtcg                                                                                                               <  2:833197/146‑1 (MQ=255)                                 ggcgggcgcccaaaaaaTGGGGAGTATTTCCCGGCGCGTACGGAAACGGGCCGCCCGGCGTCCCGGAACC‑GGGGTG‑TAT‑CTCAACCCTTAAAA‑CCGTTATCAGGATCAAAGGACCACCCCCCCCGCGGTTGCCCGCCGTTACATCGTCGaa                                                                                                             <  2:1029867/138‑1 (MQ=255)                                    cGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                          >  2:165772/1‑151 (MQ=255)                                     ggCGCCCGTAAATTGGGGTTTGATCCCTTGGGATTACTGGAAGAGGTCGCGGGGTGTCCAGGAACT‑GGGGTG‑TAT‑TCCGAACCTTTAAC‑CCGTTATGAGAATCTTATAACCACCCCCCGAGCGAAAGCCCGCCGTTACATCGTCGAAAAc                                                                                                          <  1:313880/150‑1 (MQ=255)                                        gACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATc                                                                                                      >  2:720712/1‑151 (MQ=255)                                         aCCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                     >  1:53225/1‑151 (MQ=255)                                          ccGCAAAATGGTGAGTGATCCCCTGCGCGTACTGAAACAGGTCGCCGCGCGTCCCGGACCC‑GTGGTG‑TAT‑CTCAAACCGTTAAA‑CCGCTATCAGGATAATATGATCAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGa                                                                                                        <  1:673425/147‑1 (MQ=255)                                              aaatgggTGAGTGTTCCCCGGCGGGTAGGGGAACAGTCCCCCGCGGGACCGGGACCC‑GTGTTG‑TCC‑CCCAACCCGTAAAC‑CCGCTATCAGGATAATATGAAAACCACCGCCCCCAATCCCCGCCGTTACATCGTCGAAAACGATCTTa                                                                                                   <  2:466526/143‑1 (MQ=255)                                              aaaaTGGTGAGTTTCCCCCCGCCCTAACGGAAACGGGTCCCCGCGCGGCCCGGACGC‑GGGGTG‑T‑C‑CCCCAACCCTTAAACCCGTAACCAGTTTAATAGGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                  <  2:699665/149‑1 (MQ=255)                                               aaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                >  1:571179/1‑150 (MQ=255)                                               aaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                >  1:445171/1‑150 (MQ=255)                                                   ggTGAGTGTTTCCCTGCGCTTACTGAAACGGGTCGCCGCGCGTCCCGGACCG‑GTGTTT‑TAT‑CTCGACCCTTAAAC‑CCGCTATCAGGATCATATAATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGt                                                                                            <  1:967037/150‑1 (MQ=255)                                                    gtgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                          >  1:151649/1‑151 (MQ=255)                                                    gggggtgtTTCCCGGCGCTTACTGGAACAGGCCCCCGCGCGTCCGGGACGG‑GTGGTG‑TCT‑CCCGACCCGTAAAC‑CCCCTATAAGGATCATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTa                                                                                           <  2:782683/146‑1 (MQ=255)                                                      gagtgaTCCCCGGCGCGTACTGGAACGGGTCGCCGCGCGTCCCGGACCG‑GTGGTT‑TAT‑CTCGAACCTTAAAA‑CCGCTATCAGGATAATATGACCAACACCCTCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                        <  2:480910/151‑1 (MQ=255)                                                        gtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                          >  1:871986/1‑147 (MQ=255)                                                        gtgaTCCCCGGCGCGTACTGAAACGGGTCGCCGCGCGTCCCGGACCC‑GTGGTG‑TAT‑CTCGACCCTTAAAC‑CCGCTATCAGGATCATATGATAAACCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                        <  1:644133/149‑1 (MQ=255)                                                         tgaTTCCCTGCGCGTACTGGAACGGGCCGCCGCGCGTACCGGACCC‑GTGGTG‑TAT‑CTCAACCCGTAAAC‑CCGCTATCGGGATCATATGACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                      <  1:445108/150‑1 (MQ=255)                                                          gaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                     >  2:930796/1‑150 (MQ=255)                                                            ttCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                  >  1:827558/1‑151 (MQ=255)                                                            ttCCCTGCGCGTACGGGAACAGTTCGCCGCGGGTACCGGACCC‑GGGGTG‑TAT‑CCCAACCCGTAAAC‑CCGCTATCAGGATAATATGATCAACCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                    <  1:839091/149‑1 (MQ=255)                                                              cctgccgcgTACTGGAAGTGGCCGGCGGGCTTACCGCACCC‑G‑GGTGTTAT‑CTAAAGCCTTTAAA‑CCCCTTCAAGGATAATATGACAACCCCCCTGGCTGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                <  1:933372/146‑1 (MQ=255)                                                              cccTGCGTGTAGGGGAACAGGCCGCCGCGCGTACCGG‑ACG‑GGGGGT‑TATGCTCAACCCGTAAAA‑CCGTAAACGGGATAATAGAAACAACCCCCTCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                      <  2:505053/145‑1 (MQ=255)                                                                   cgcgTCCGGAAACAGGTCGCCGCGCGTGCGGGAACC‑GGGGTG‑TAT‑CTCGAACCTTAAAC‑CCGTTATAAGGACCATATGACAAACCCCCTCGCGGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAt                                                                            <  1:743406/150‑1 (MQ=255)                                                                      gTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                        >  2:630398/1‑151 (MQ=255)                                                                             aaCGGGTCGCCGCGCGT‑CCGGAACCGGTGGTG‑TAT‑CTCGAACCGTAAAC‑CCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcaca                                                                  <  1:650555/150‑1 (MQ=255)                                                                              aCAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                >  1:1045082/1‑151 (MQ=255)                                                                                 ggTCGCCGCGCGCCCCGGAACC‑GTGTTT‑TAG‑CTCGACCCTTTAAC‑CCGCTAAAAGAAACATATAACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                             <  1:371532/151‑1 (MQ=255)                                                                                       cgcgcgTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATc                                                          >  1:271412/1‑148 (MQ=255)                                                                                       cgcgcgCCCCGGACCC‑GTGGTG‑TCT‑CCCGACCCTTAAAC‑CCGCTATAAGTAAAATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAatat                                                           <  2:989572/147‑1 (MQ=255)                                                                                                cgaaaCC‑GTGGTG‑TAT‑CTCAAACCTTTAAA‑CCGTTACCAGAATAATAGGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATa                                                <  1:255348/146‑1 (MQ=255)                                                                                                    accggtggtt‑TAT‑CTCG‑ACCATTAAACCCCCAATCAGGATCATATGAACAACCCCCCCGCCGAGCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                            <  1:366354/146‑1 (MQ=255)                                                                                                     cc‑GTGGTG‑TAT‑CTCGAACCTTTAAC‑CCGCTATCAGGATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                          <  2:975478/150‑1 (MQ=255)                                                                                                            tg‑tAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                   >  2:338182/1‑151 (MQ=255)                                                                                                             g‑tAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                  >  1:966099/1‑151 (MQ=255)                                                                                                               tAT‑CTCGACCCTTTAAC‑CCGCTATAAGGATAATATAATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                   <  2:1043619/149‑1 (MQ=255)                                                                                                                   ccggAACCTTAAAC‑CCGTTACAAGGATCATATAACAAACCCCCGCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGc                              <  2:982490/148‑1 (MQ=255)                                                                                                                      gAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                           >  2:928244/1‑151 (MQ=255)                                                                                                                       accgtttAAC‑CCGTTATCAGTTTCATATGACAAACCCCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                           <  1:249950/145‑1 (MQ=255)                                                                                                                       accctttAAC‑CCGCTATAAGAATAATATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                            <  1:790940/144‑1 (MQ=255)                                                                                                                        aCCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGCTTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGa                          >  2:793443/1‑150 (MQ=255)                                                                                                                          cGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACa                        >  2:89321/1‑150 (MQ=255)                                                                                                                            ttAAC‑CCGTTATCAGGATCATTTGACAAACCCCCCCGCCAAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACaa                       <  2:511530/149‑1 (MQ=255)                                                                                                                             tAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                    >  2:110956/1‑151 (MQ=255)                                                                                                                             tAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                    >  2:443731/1‑151 (MQ=255)                                                                                                                               aacccGCTATAAGGTTAATATGACCAACCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAcc                     <  2:1057097/146‑1 (MQ=255)                                                                                                                                   cGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAcc               >  2:867009/1‑151 (MQ=255)                                                                                                                                      tATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGc            >  2:47487/1‑151 (MQ=255)                                                                                                                                         cAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg         >  2:417360/1‑151 (MQ=255)                                                                                                                                           ggATAATATGACCAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc          <  2:215751/148‑1 (MQ=255)                                                                                                                                            gATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc      >  2:57514/1‑151 (MQ=255)                                                                                                                                              tCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt    >  1:812587/1‑151 (MQ=255)                                                                                                                                               cATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg   >  2:436535/1‑151 (MQ=255)                                                                                                                                               cATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg   >  2:326968/1‑151 (MQ=255)                                                                                                                                                atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt  >  2:291763/1‑151 (MQ=255)                                                                                                                                                atatGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt    <  2:430166/149‑1 (MQ=255)                                                                                                                                                 tataaccAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg   <  2:1054409/143‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                            CGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTG‑TAT‑CTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGT  >  NC_000913/1376355‑1376644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑