Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,248,4310AC41.8% 84.7 / 17.0 56G34G (GGT→GGGlysPlysine transporter
Reads supporting (aligned to +/- strand):  ref base A (24/8);  new base C (0/23);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 4.74e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGAT  >  NC_000913/2248287‑2248581
                                                                                                                                                |                                                                                                                                                       
aaaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGt                                                                                                                                                    >  1:921367/1‑150 (MQ=255)
    gAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCg                                                                                                                                               >  1:1034616/1‑150 (MQ=255)
        cGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATgg                                                                                                                                           >  1:226976/1‑150 (MQ=255)
          gAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAAc                                                                                                                                        <  2:580491/151‑1 (MQ=255)
          gAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAAc                                                                                                                                        <  2:716254/151‑1 (MQ=255)
           aaaCCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAAcc                                                                                                                                       <  1:410741/151‑1 (MQ=255)
             aCCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGc                                                                                                                                     <  2:660900/151‑1 (MQ=255)
                ggCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCa                                                                                                                                   >  1:935662/1‑150 (MQ=255)
                ggCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCa                                                                                                                                   >  1:629919/1‑150 (MQ=255)
                             ccAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGt                                                                                                                     >  1:1038296/1‑151 (MQ=255)
                               atttCCCCGAGCCTGGTCATCAGAAAGAAACCCACCGGCCCAACAAGAATATACGAGAGAAATCCCCCCCCGGCCCCTCCCTGAAAAATCTTTCCGCCAGAGCAAAAAAAAAGCCCTGTCCCGAGGAAACCGCCAATGGCAATCATCGTCa                                                                                                                   <  1:891001/149‑1 (MQ=255)
                                 ttCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAgg                                                                                                                 >  2:871081/1‑151 (MQ=255)
                                   cACCGAGACTGGTCATAAGAAAGTAACCCATCAGGCCAATCAGCATATACGAGAGAAATCCCCCCCCCGGACCTGCCTGAAAAATCGTGGCGCCAGAGGAAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTg                                                                                                               <  1:826969/151‑1 (MQ=255)
                                         gaCTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACgcgc                                                                                                         >  1:144046/1‑151 (MQ=255)
                                             gttcatcaGGAAAAAACCCACCAGGCCAACCAGCATATACGAGAAAAATCCCCCGCCCGGACCTGCCTGAAAAATCGTTGCGCCAGAGGAAAAAAAAAGCCCTGTCCCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGAcgc                                                                                                           <  2:951759/143‑1 (MQ=255)
                                             gttcataggaaaGAAAACCATCAGGCAAACAAAAATAGCCGAGAGCAATCCCCCGCCGGCCCCTCCCTGAAAAATCGTGGCGCCAGAGACAAAAAAAAGCCCTGCCCCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCttt                                                                                                     <  2:514941/141‑1 (MQ=255)
                                                catcaGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAAt                                                                                                  >  1:668515/1‑151 (MQ=255)
                                                 atcaGGAAGAAACCCACCGGGCAAAACAACATAAAGGAGACAAACCCCCCGCCCGGCCCTCCGTGAAAAACCGTGGCGCAAGAGAAAAAAAAAAGCCCTTTCCGGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTaa                                                                                                   <  2:40861/149‑1 (MQ=255)
                                                    agaaaGAAAACCACCAGCCCAATCAGAATATACGAGAGCATTCCCCCGCCCGGCCCTGCCTGAAAAATCGTTGCGCAAGAGGCAAAAAAAAGCCCTGTACCTATGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAAtt                                                                                                 <  2:391990/145‑1 (MQ=255)
                                                       aaataaccctacggaccAATCAAAATATACAAGACAAATGCCCCACCCGGCCCTGCCTAAAAATTTGTTGCGCAAGATGCAAAAAAAGGCCCGTTCCGGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGc                                                                                           <  2:284285/136‑1 (MQ=255)
                                                              ccATCGGGCAAAACACCAAATAGGAGAGCAACGCCCCCCCCGGCCCTGCTGGAGAAATCGTTGCGCCAGAGGAAAAAAAAAGCCCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGcc                                                                                    <  2:935652/151‑1 (MQ=255)
                                                                         accAGAATATCCGAGACAAACGCCCCCCCCGGCCCGGCATAAAAAATCGTTGCCCCAGAGACAAAAAAAAGCCCTGTCCCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCt                                                                          <  2:835239/148‑1 (MQ=255)
                                                                         aTCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCtg                                                                         >  2:558990/1‑151 (MQ=255)
                                                                         aTCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCtg                                                                         >  2:221485/1‑151 (MQ=255)
                                                                                tataCGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGCGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGtttt                                                                  >  2:1002868/1‑151 (MQ=255)
                                                                                   acaagacCAATCCCCCCCCCGGCCCTGCCTGAAAAACCGTGGCGCCAGGGCAAAAAAAAAGCCCTGTCCGGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGtttt                                                                  <  2:871017/145‑1 (MQ=255)
                                                                                   aCGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGt                                                               >  2:340201/1‑151 (MQ=255)
                                                                                        aactgccccccGCCGGGCCCAGCCGGAGAAGGGGTGGCGCCGGAGCAAAAAAAAAGACCTGTCCTATTGGACCCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGt                                                               <  2:904605/139‑1 (MQ=255)
                                                                                           aacgCCCCGCCCGGCCCTGCCGGAGAATTCGTTGCGCAAGGGCCAAAAAAAAGCCCTGTCCCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGaa                                                        <  1:528128/147‑1 (MQ=255)
                                                                                           aaccccccGCCCGGCCCTGCCGGAAAAATCGTTGCGCCAGAGGAAAAAAAAAGCCCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGaa                                                        <  1:454186/146‑1 (MQ=255)
                                                                                           aaTGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGaa                                                        >  1:40769/1‑150 (MQ=255)
                                                                                           aaTCCCCCGCCCGGACCTGCTTGAAAAATCGTGGCGCCAGAGGAAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGa                                                         <  1:626982/149‑1 (MQ=255)
                                                                                               ccccccccGGCCCTGCCAGAAAAATCGTTGCGCCAGAGACAAAAAAAAGCCCTGTCCCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCa                                                    <  1:180473/150‑1 (MQ=255)
                                                                                               ccccGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCAt                                                   >  1:772918/1‑151 (MQ=255)
                                                                                                 ccgccggAACCTGCATGAAAAATCGTGGCGCAAGAGGAAAAAAAAGGCCTTGTCCCGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCa                                                    <  2:806273/148‑1 (MQ=255)
                                                                                                       ggACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAAt                                           >  1:369532/1‑151 (MQ=255)
                                                                                                        gACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATg                                          >  1:843955/1‑151 (MQ=255)
                                                                                                        gACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATg                                          >  2:145991/1‑151 (MQ=255)
                                                                                                          cctgcctgAAAAACCTTTGCGCCAGAGAAAAAAAAAACACCTGTACCGATGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATg                                          <  1:86872/149‑1 (MQ=255)
                                                                                                          ccgccctaaaaaaTCTTGGCGCCAGAGCAAAAAAAAAGCCCTGCACCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATg                                          <  2:9351/139‑1 (MQ=255)
                                                                                                             gcctgAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATc                                     >  1:882286/1‑151 (MQ=255)
                                                                                                              cctgAAAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATc                                     <  1:643909/150‑1 (MQ=255)
                                                                                                                  aaaaaTCGTTGCGCAAGAGGAAAAAAAAAGCCCGTTACGGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATcc                                    <  2:993172/145‑1 (MQ=255)
                                                                                                                   gaAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCtt                               >  1:663262/1‑151 (MQ=255)
                                                                                                                    aactcgtcccgcgAGAGAAAAAAAAAAGCCCTGTCCGGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTa                              <  1:870262/142‑1 (MQ=255)
                                                                                                                    aaacggTTGCGCCAGAGCAAAAAAAAAGCCCGGTACGGATGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCtt                               <  2:959810/145‑1 (MQ=255)
                                                                                                                    aaaTCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCtt                               >  1:548667/1‑150 (MQ=255)
                                                                                                                    aaaTCGTGGCCCCAGAGGCAAAAAAAAGCCCTGTCCCGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCtt                               <  2:946506/150‑1 (MQ=255)
                                                                                                                     aaTCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGAGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTa                              >  2:676818/1‑150 (MQ=255)
                                                                                                                         tgtGCGCGAGGGCCAAAAAAATGACCTGCACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTaa                             <  2:194536/145‑1 (MQ=255)
                                                                                                                             cgcAAGAGGAAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTaaaaa                          <  1:245874/146‑1 (MQ=255)
                                                                                                                                   agcaaaaaaaaaCCCCTGTCCAGAGGAACCCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGa                <  2:1034602/143‑1 (MQ=255)
                                                                                                                                       aaaaaaaaGCCCTCTCCGGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCTCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTa           <  2:416810/148‑1 (MQ=255)
                                                                                                                                       aaaaaaaaGACCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGAtt              <  1:424262/145‑1 (MQ=255)
                                                                                                                                           aaaaGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGc         >  1:926419/1‑149 (MQ=255)
                                                                                                                                             aaGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGAc     >  2:39469/1‑151 (MQ=255)
                                                                                                                                                aCCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGAt  >  2:105214/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                       
AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGAT  >  NC_000913/2248287‑2248581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: